Tecido hepático e corpo adiposo de rã-touro (Lithobates catesbeianus) submetido a diferentes fotoperíodos

The study was conducted to evaluate the liver tissue weight, body fat weight, the fatty somatic index and the liver somatic index of bullfrogs subjected to three photoperiod during the growing phase (initial 30 days) and finishing (60 days). The treatments were 16 h light and 8 h dark, 12 h of light and dark and 8 h light and 16 h dark. The experimental design was completely randomized design with three replications. We used 36 bullfrogs/m2 weighing from 22 to 28 g. The results were submitted to ANOVA and Tukey Test at 5% probability. Differences were found for the weight of body fat, liver tissue weight, liver somatic index and fatty somatic index the bullfrog to the treatments and the study periods and there is greater accumulation of energy to ninety days. The photoperiod did not influence body weight and fat weight of the liver tissue of bullfrog at the end of the fattening period and the difference fatty somatic index and liver somatic index were influenced by body weight of animals.

Densidade de estocagem para produção de Acará-bandeira em viveiros escavados em policultivo com Camarão-da-amazônia

The aim of this study was to evaluate the production of Freshwater Angelfish (Pterophyllum scalare) in different stocking densities in cages polycultured with Amazon River Prawn (Macrobrachium amazonicum). Four treatments were evaluated (1; 2; 3 and 4 fish. 10 L(-1)) using two ponds and 24 experimental units. Water quality parameter reached adequate levels for the species. Production data were submitted to ANOVA. Fish produced in the lowest density showed higher values of final weight and standard length, and lower total biomass in comparison with the others treatments. Feed conversion rate, survival, batch uniformity and body factor were not significant different among treatments. The highest density tested produced more fish and gross yield than the others treatments. The density 20 fish. 10L(-1) is recommended to produce Freshwater Angelfish in cages polycultured with Amazon River Prawn.

Activity of sesame leaf extracts to the leaf-cutting ant Atta sexdens rubropilosa (Hymenoptera : Formicidae)

Crude extracts of new and old sesame leaves (Sesamum indicum, Linaeus) obtained by a sequential process of extraction were tested through contact experiments to investigate their toxicity to Atta sexdens rubropilosa (Forel) workers. Methanol extract of old leaves was toxic to leaf-cutting ants at the lower tested concentration (20mg.ml(-1)). This extract was fractioned and their fractions were tested showing a toxic effect of the methanol fraction. The methanol fraction was fractioned again and the sub fraction responsible for worker's toxicity is composed of some sugars.

Fixação biológica do nitrogênio e crescimento de plantas de leucena (Leucaena leucocephala (Lam.) de wit.) sob salinidade

This study aimed to evaluate the effects of salinity in the growth and nitrogen fixation in leucaena. Plants were cultivated in Leonard pots containing clean and sterilized sand. The treatments were distributed in a completely randomized design, in a 3x3 factorial, with five replicates. The first factor corresponded to no inoculation and inoculation with Bradyrhizobium sp SEMIA 6070 and SEMIA 6153 strains. The second factor corresponded to NaCl concentrations: 0, 25 and 50 mol m(-3). Were analyzed the height and dry matter accumulation, the number of nodules and nitrogen in plants. The inoculation of plants with the strain SEMIA 6070 provided higher growth and greater nitrogen accumulation in plants thar were not subject to salinity. Nodulation was not affected by salinity. Inoculation with SEMIA 6153 strain provided greater tolerance to salinity.

Crescimento e nutrição de plantas de leucena (Leucaena leucocephala (Lam.) R. de Vit) sob salanidade

Excessive salt can to promote water soil retention, reducing your availability to plants. Besides, they can interfere in protoplasm metabolism. The plants ability to survive in salinity conditions is an important factor to geographic distribution and agriculture in salinized regions. The objective of this work was to verify the effects of the NaCl in the growth, dry matter distribution and N, K, Ca, Mg, Na, and Cl content in young plants. The experiment was conducted in 'Leonard' pots, with nutrient solutions, with NaCl ( 0, 25, 50 and 100 mmol L(-1)). Increasing of NaCl concentration reduces growth and total dry matter. There was an increase of N and K content, in shoots, and N and Mg content in roots. There was an reduction in Mg content in shoots, and reduction of Ca content in roots with increasing of NaCl. Leucaena plants were inefficient to exclude Na and Cl, in shoots principally.

Ocorrência da Mosca-negra-dos-citros (Aleurocanthus woglumi ashby) (Hemiptera: aleyrodidae) em Pernâmbuco

This paper aimed to verify the presence of black-fly of citrus in the state of Pernambuco in Brazil and wordwide. The material with symptoms were collected in the metropolitan area of Recife/PE and subsequently led to the identification in the Insect Biology Laboratory of the Federal Rural University of Pernambuco - UFRPE on February 4, 2011. The samples collected from the branches of citrus and bilocular, found the presence of eggs, nymphs, "pupae" and adults of Aleurocanthus woglumi, confirming its occurrence in the state of Pernambuco. The presence of blackfly of citrus had been detected in the city in the state of Pernambuco as Timbauba, Bom Jardim, Macaparana, Axes, Orobo, Sao Vincent Farrer and Itambe in citrus plants. Before that had a suspect in the Valley Siriji/PE, now it is in the metropolitan area of Recife, therefore we can confirm that the material collected and identified in the metropolitan area of Pernambuco is A. woglumi.

Não preferência para alimentação e aspectos biológicos de Spodoptera eridania em cultivares de feijão-caupi

The aim of this paper was to evaluate the no-preference for feeding and biological aspects of Spodoptera eridania fed on cowpea cultivars BR17 Gurgueia, BRS Urubuquara, BRS Nova Era, Sempre Verde, BRS Milenio and BR3 Tracuateua. In free-choice test, leaf discs were placed in Petri dishes where one third instar larvae per cultivar was released, whereas in no-choice test one leaf disc was placed per Petri dish where one caterpillar per cultivar was released, evaluating their attractiveness after 1, 3, 5, 10, 15, 30, 60, 120, 360 and 720 minutes, as well as the leaf area consumed. Randomized blocks and complete randomized blocks design were used for free-choice and no-choice tests, respectively, with six treatments and 10 replications. The evaluation of the biological parameters of S. eridania was carried out in Petri dishes where recently hatched caterpillars were transferred in the proportion of one per dish, and the leafs of the cultivars were offered to them during the whole larval period, and we evaluated: periods and viabilities of larvae and pupae, overall viability, weight of larvae and pupae, sex ratio, longevity and overall cycle. Complete randomized design was used with six treatments and 30 replications. In no- preference for feeding free-choice test the cultivars Sempre Verde and BR17 Gurgueia were the most and the least consumed, respectively. Regarding the effects of cowpea cultivars on larval viability we can infer that BRS Urubuquara and Sempre Verde show antibiosis-type resistance to S. eridania.

Técnica de criação e parâmetros biológicos de Stegasta bosquella em amendoim

Studies with Stegasta bosquella in peanut crop are little explored by researchers, mainly by the need of obtaining, in the field, a large quantity of closed leaflets. Therefore, it was sought a way of rearing in a natural diet that facilitates the attainment of the insect. The research was developed at the Laboratorio de Resistencia de Plantas a Insetos (FCAV/Jaboticabal) (T: 25 +/- 2 degrees C, RH 60 +/- 10% and photophase: 12 hours) (Runner IAC 886). The rearing stock of was carried out by collecting caterpillars from the field and keeping them in flat bottom glass tubes until adult emergence and test performance. Caterpillars were individualized in Petri dish plates, lined with moistened filter paper, which contained closed and early opened leaflets, but closed with paper clips (imitating the closed leaflet). A randomized design with two treatments and 36 repetitions was used. After the pupae formation, they were separated into five couples and the adults were kept in transparent plastic cages containing a peanut stem with leaves for oviposition, and fed with a 10% honey solution. The duration and viability of the larval and pupal periods, male and female longevity with and without food, and fertility were evaluated. The two forms of larvae rearing (closed leaflet and leaflet closed with clips) did not influence on any of the studied parameters. Therefore, the rearing of S. bosquella becomes feasible in natural diet, which means there is no need to use the enclosed leaflets for this purpose, and implies the easiness for rearing.

Subdosages of Bacillus thuringiensis on Plutella xylostella) (Lepepidoptera: plutellidae) and Trichogramma pretiosum (Hymenoptera: trichogrammatidae)

The aim of this paper was to evaluate the biological aspects of Plutella xylostella and Trichogramma pretiosum in eggs of the F2 generation of P. xylostella under the influence of Bacillus thuringiensis in laboratory conditions. The experiment was conducted in the Laboratorio de Biologia and Criacao de Insetos of Faculdade de Ciencias Agrarias e Veterinarias de Jaboticabal - UNESP reen collars contaminated with strains and commercial product based on B. thuringiensis in the laboratory. The eggs obtained from the F2 generation of P. xylostella evaluated the biological parameters of T. pretiosum. It was observed that some biological characteristics of P. xylostella showed changes by the treatment with B. thuringiensis. The viability of the larvae and pupae stages, pupae weight were the biological parameters more influenced by treatments, with values significantly reduced when compared to control. However, the larvae length and pupae stages and sex ratio were similar in all treatments, with no significant biological variations. Thus, this bacterium isolated from this behavior may provide greater exposure of larvae to other natural enemies as well as generation of adults less viable, which makes them potential programs in pest control, since the interaction of the methods of control is one of the main ways to enhance the biological control of insect pests. It was observed sublethal effects on P. xylostella biology, and B. thuringiensis negative influence on the parasitism capacity and emergency of T. pretiosum.

Fecundity and pre-imaginal period of Chrysoperla externa (hagen) (neuroptera: chrysopidae) under different populations and laboratory generations

The lacewings appear in many agroecosystems, preying several species of agricultural pests. They have great search capability, high voracity, high reproductive potential and are easily maintained in laboratory conditions. In laboratory rearing, to avoid problems in the mass production are recommended adjustments in the type and quality of prey to be used in order to obtain individuals with desirable characteristics. It is necessary special care with the laboratory populations, avoiding problems from inbreeding. Larvae were reared individually in Petri dishes (9.0 cm diameter) and fed with eggs of Sitotroga cerealella (Olivier, 1789) (Lepidoptera: Gelechiidae), in the amount of 25 mg / larva, while the adults were kept in PVC cylindrical cages (10 cm x 30 cm). Thus, the study analyzed the influence of the size of the population of Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae) on the pre-imaginal period (egg to adult) and reproductive capacity of this specie come from different populations and generations of laboratory. To this end, we used two populations, one of Jaboticabal (F-8 and F-21) and one of Piracicaba (F-6 and F-15), and subpopulations of 1, 5, 10, 15 and 20 couples, analyzing the incubation of eggs and the number of eggs per female in each population, generation and subpopulation. The pre-imaginal period (egg to adult) and the number of eggs per female of C. externa are influenced by the generation and the number of founding individuals, being these parameters favored when laboratory populations are established with the largest number of couples.

Diamondback moth oviposition nonpreference in cauliflower genotypes

The Diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the main plague-insect specie of Brassicaceae plants in Brazil and all over the world. The resistant genotypes use to its control is a promising alternative. This work aimed evaluates the eggs distribution along the plant, the adults' density per plant, and determine the cauliflower genotypes effect in the P. xylostella oviposition. The experiment was carried out at FCAV/UNESP-Jaboticabal Campus Phytossanity Department (Departamento de Fitossanidade). It was evaluated the eggs distribution, the P. xylostella adults density effect using Sharon hybrid, and tests with or without choose choice to determine the P. xylostella nonpreference in the Teresopolis Gigante, Verona, Barcelona, Sharon, Silver Streak, and Piracicaba Precoce genotypes. It is possible conclude that P. xylostella has higher willingness to oviposits in the stem than in the basal leaves. The three couple density of P. xylostella per plant is the best to discriminate cauliflower genotypes regarding the resistance grade to nonpreference choose choice to oviposition. During the P. xylostella oviposition preference tests with choose choice, the genotypes Sharon, Piracicaba Precoce, Barcelona, Verona e Teresopolis Gigante are less desirable to oviposition; while during the no choose choice tests the genotypes did not differ among them.

Resistência de genótipos de feijão-vagem ao ataque de bruquíneos, em condições de laboratório

The aim of this paper was to determine resistance types of snap beans genotypes under infestation of bruchins Zabrotes subfasciatus (Bohemann, 1833) of Acanthoscelides obtectus (Say, 1831) in a no choice and free choice tests. The treatments were arranged in a completely randomized design with four and five replications, respectively. In no choice test, it was evaluated the total number of viable and unviable eggs, dry weight of the consumed food, percentage of emerged insects, weight of insects, longevity of adults and biological cycle of egg to adult. In a free choice test, the number of attracted insects for each genotype and total number of eggs were evaluated. The genotype ARFVI047 presents oviposition non-preference resistance type to Z. subfasciatus. The genotype ARFVI008 presents resistance type of the antibiosis to Z. subfasciatus. The genotypes ARFVI006, ARFVI008 and ARFVI029 present resistance of non-preference for oviposition type to Z. subfasciatus and the genotype HAV 56 black seed presents resistance of non-preference type for feeding of A. obtectus.

Escarificação ácida na superação da dormência de sementes de pau ferro (Caesalpinea ferrea Mart.ex Tu. var. leiostachya Benth.)

Seeds of Caesalpinea ferrea Mart. ex Tu. var. leiostachya Benth. present low and irregular germination due to dormancy caused by the impermeability of the tegument. With the purpose to determine an efficient method to accelerate and uniformize seed germination, they were submitted to different immersion periods (0, 5, 10, 15, 20, 25 and 30 minutes) in sulfuric acid (95-98%), influence emergency and vigor (first count, speed index, medium time and relative frequency of emergency, length and dry matter of the seedlings). The experiment was carried out under greenhouse conditions with an experimental design of completely randomized, with seven treatments and four replications. The pre-conditioning of the seeds with immersion in concentrated sulfuric acid was efficient to overcoming seed dormancy, by increasing the percentage and speed emergency, the first count of emergency, length and dry matter of seedlings and reduction in the medium time for emergency. The efficiency of this chemical treatment with concentrated sulfuric depends on the immersion time, and 10 to 20 minutes were the most adequate to provide larger emergency uniformity percentages and vigor.

Consorciação e plantas daninhas afetando a produtividade e a qualidade de sementes de sorgo

This research aimed to study competition effects of legumes intercropping and weeds on seeds yield and quality of Sorghum bicolor (L.) Moench. The experiment was carried out in a Rhodic Nitisol, under field condition in Botucatu region, São Paulo State, Brazil. Ten treatments were tested: sorghum (cv. A-6304) intercropped with Dolichos lablab L., Mucuna aterrima (Piper et Tracy) Holland, Canavalia ensiformis D. C. or Crotalaria juncea L., that were sown in the same time of sorghum or 20 days after the sorghum sowing, and sorghum weed-free and sorghum without weeding. The experimental design employed was the completely randomized blocks with four replications. There were evaluated sorghum seeds yield and some characteristcs of seed quality: purity, hectolitric weight, 1000 seeds weight, insect infestation, germination and vigor (by first counting of germination test and accelerated ageing test). The competition caused by legumes intercropping and weeds affected the yield and the physical and physiological characteristics of sorghum seeds, but the intensity of prejudicial effects depended on the time and the species of legume intercropping.

Densidade populacional e época de plantio no crescimento e produtividade da couve-flor cv. verona 284

To study plant growth and yield of cauliflower, two field trials were carried out: the first spring-summer (Oct. 07, 2006 to Jan. 28, 2007) and the second autumn-winter (Apr. 04, 2007 to Jul. 09, 2007). The experimental design was randomized complete blocks in 4 x 4 factorial design with three replications. The following factors: line spacing (0.6, 0.8, 1.0 and 1.2 m) and plant spacing (0.4, 0.5, 0.6 and 0.7 m). The used cultivar was cv. Verona 284. The characteristics, number of leaves by plant, diameter of stem, diameter of inflorescence, inflorescence mass and yield were evaluated. With the reduction in spacing, were observed lower numbers of leaves, stem diameter, diameter and mass of the inflorescence, but there was an increase in yield. The maximum yield (23 t ha(-1)) was obtained with 0.6 x 0.4 m, ie, in higher plant population (41,667, plants ha(-1)).