O apelo ao legislador : apellentscheidung, na praxis da Corte Constitucional Federal Alemã / Gilmar Ferreira Mendes. --Tribunal Constitucional Alemão e o apelo ao legislador : "appelentscheidung" ; na praxis da Corte Constitucional Federal Alemã. Revista de Direito Público, vol 25 n 99 p 32 a 53 jul/set 1991."

Variação do título : Apelo ao legislador na Corte Constitucional Federal Alemã. Revista Trimestral de Direito Público, n 10 p 33 a 51 1995.

Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis

Cells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role.

ANIBAL, stable isotope-based quantitative proteomics by aniline and benzoic acid labeling of amino and carboxylic groups

Identification and relative quantification of hundreds to thousands of proteins within complex biological samples have become realistic with the emergence of stable isotope labeling in combination with high throughput mass spectrometry. However, all current chemical approaches target a single amino acid functionality (most often lysine or cysteine) despite the fact that addressing two or more amino acid side chains would drastically increase quantifiable information as shown by in silico analysis in this study. Although the combination of existing approaches, e.g. ICAT with isotope-coded protein labeling, is analytically feasible, it implies high costs, and the combined application of two different chemistries (kits) may not be straightforward. Therefore, we describe here the development and validation of a new stable isotope-based quantitative proteomics approach, termed aniline benzoic acid labeling (ANIBAL), using a twin chemistry approach targeting two frequent amino acid functionalities, the carboxylic and amino groups. Two simple and inexpensive reagents, aniline and benzoic acid, in their (12)C and (13)C form with convenient mass peak spacing (6 Da) and without chromatographic discrimination or modification in fragmentation behavior, are used to modify carboxylic and amino groups at the protein level, resulting in an identical peptide bond-linked benzoyl modification for both reactions. The ANIBAL chemistry is simple and straightforward and is the first method that uses a (13)C-reagent for a general stable isotope labeling approach of carboxylic groups. In silico as well as in vitro analyses clearly revealed the increase in available quantifiable information using such a twin approach. ANIBAL was validated by means of model peptides and proteins with regard to the quality of the chemistry as well as the ionization behavior of the derivatized peptides. A milk fraction was used for dynamic range assessment of protein quantification, and a bacterial lysate was used for the evaluation of relative protein quantification in a complex sample in two different biological states

Kalan ylimmät vähittäismyyntihinnat voimassa heinäkuun 12 p:stä 1942 toistaiseksi

32 x 33 cm

Kalan ylimmät vähittäismyyntihinnat voimassa joulukuun 20 p:stä 1942 toistaiseksi

32 x 33 cm

Kalan ylimmät vähittäismyyntihinnat voimassa toukokuun 11 p:stä 1943 toistaiseksi

32 x 33 cm

Kalan ylimmät vähittäismyyntihinnat voimassa heinäkuun 7 p:stä 1943 toistaiseksi

32 x 33 cm

ALBUM AMICORUM de Jean Durand (1583-1592). In-8 de 2 f. lim. et 380 p., mar. r., comp. de mos. de mar. v., riche dorure à petits fers, tr. dor. et ciselée. (Rel. du XVIe siècle.)

Durand (Jean). Album amicorum (1583-1592)

Recueil de lettres de P. de Marca, adressées, pendant son séjour en Catalogne, principalement à Michel Le Tellier. Du 4 janvier 1650 au 30 juillet 1651. Copies.

Contient : 1 à 8 Huit lettres de P. DE MARCA à M. Le Tellier. Des 4, 12, 17, 19, 28 janvier, 1er février 1650 ; 9 « Relation de ce qui a esté faict en execution du contenu aux instructions de S. M. [LOUIS XIV], du 18 janvier 1650, envoyées au Sr de Marca » ; 10 à 30 Vingt et une lettres de P. DE MARCA ; 10 à 22 à M. Le Tellier. Des 1, 4, 5, 9, 25, 26 février, 2, 6, 9, 15, 23, 30 mars 1650 ; 23 « à Mrde Brienne. Du 2 avril 1650 » ; 24 et 25 à M. Le Tellier. Des 20 et 29 avril 1650 » ; 26 au cardinal Mazarin. Du 29 avril 1650 ; 27 à 30 à M. Le Tellier. Des 3, 4, 18, 25 mai 1650 ; 31 Placet adressé au roi LOUIS XIV par la comtesse DE ÇAVALLA, touchant les droits de cette dame aux revenus de la baronnie de Belpuech ; 32 à 38 Sept lettres de P. DE MARCA ; 32 et 33 à M. Le Tellier. Du 1er juin 1650 ; 34 au « duc de Mercoeur. Le 28 may 1650 » ; 35 à 38 à M. Le Tellier. Des 8 et 12 juin, 6 juillet 1650 ; 39 « Relation de ce qui s'est passé à Barcelone contre les conjurez. Du 12 juillet 1650 » ; 40 et 41 Deux mémoires de P. DE MARCA ; 40 « sur les divisions de Catalogne, et du remede qui s'y peut apporter » ; 41 « touchant les biens confisquez. Du 5 juillet 1650 » ; 42 « Lettre de P. DE MARCA à M. Le Tellier. Du 13 juillet 1650 » ; 43 « Project de la declaration » du roi « LOUIS [XIV], pour la revocation des dons des biens confisquez en Catalogne » ; 44 à 51 Huit lettres de P. DE MARCA ; 44 à 48 à M. Le Tellier. Des 19 et 26 juillet, 2 et 16 août 1650 ; 49 au cardinal Mazarin. Du 16 août 1650 ; 50 et 51 à M. Le Tellier. Des 23 et 30 août 1650 ; 52 « Relation de la prise de Falset. Du 29 aoust 1650 » ; 53 « Memoire du 29 d'aoust 1650 », sur les affaires de Catalogne ; 54 à 59 Six lettres de P. DE MARCA à M. Le Tellier. Des 6, 13, 14 septembre 1650 ; 60 « Estat des affaires de Catalogne. Du 2 octobre 1650 » ; 61 à 74 Quatorze lettres de P. DE MARCA ; 61 et 62 à M. Le Tellier. Des 4 et 11 octobre 1650 ; 63 au cardinal Mazarin. Du 11 octobre 1650 ; 64 à 68 à M. Le Tellier. Des 16 et 18 octobre, 1er, 2 et 8 novembre 1650 ; 69 « à MrOndedei. Du 8 novembre 1650 » ; 70 à 74 à M. Le Tellier. Des 8, 15, 22 novembre 1650 ; 75 « Lettre de Mr DE CANILLAC à Mr de Marca. Du 11 novembre 1650 » ; 76 à 81 Six lettres de P. DE MARCA à M. Le Tellier. Des 24 et 29 novembre, 5, 6, 12, 13 décembre 1650 ; 82 « Memoire de Mr DE MARCA », sur les affaires de Catalogne. Du 17 décembre 1650 ; 83 Lettre de P. DE MARCA à M. Le Tellier. Du 22 décembre 1650 ; 84 « Memoire de Mrs DE ST MEGRIN, DE MARCA, DE MARGARIT et LECLERC, touchant l'estat de la Catalogne. Du 22 decembre 1650 » ; 85 à 97 Treize lettres de P. DE MARCA ; 85 « à MrOndedei. Le 22 decembre 1650 » ; 86 à 97 à M. Le Tellier. Des 26 decembre 1650, 3, 10, 14, 22, 24, 31 janvier, 4, 7 et 14 février 1651 ; 98 « Memoire de [P.] DE MARCA sur la conduitte de Mr l'evesque d'Orange » ; 99 Lettre de P. DE MARCA à M. Le Tellier. Du 21 février 1651 ; 100 « Relation de ce qui s'est passé en l'entreprise que les ennemis ont voulu faire sur Prades en Catalogne » ; 101 à 105 Cinq lettres de P. DE MARCA à M. Le Tellier. Des 28 février, 7, 14 mars 1651 ; 106 « Memoire du 14 mars 1651, sur la conduite de Mrs l'evesque d'Orange, comte d'Ille et regent Fontanella » ; 107 à 134 Vingt-huit lettres de P. DE MARCA à M. Le Tellier. Des 27 mars, 4, 12, 23 avril, 3, 6, 14 à 16, 21, 27, 31 mai, 7, 16 à 18, 21, 30 juin, 1, 10, 24, 30 juillet 1651

Noticias de la vida y escritos del Rmo. P. Mro. Fr. Henrique Flórez de la Orden del gran P. S. Augustín... : con una relacion individual de los viages que hizo a las provincias y ciudades mas principales de España /

Apendices de p. 277 a 350. De p. 277 a 350, texto en latín y en español.

Data on the biogeochemical cycle of methane in the ocean

Geological, mineralogical and microbiological aspects of the methane cycle in water and sediments of different areas in the oceans are under consideration in the monograph. Original and published estimations of formation- and oxidation rates of methane with use of radioisotope and isotopic methods are given. The role of aerobic and anaerobic microbial oxidation of methane in production of organic matter and in formation of authigenic carbonates is considered. Particular attention is paid to processes of methane transformation in areas of its intensive input to the water column from deep-sea hydrothermal sources, mud volcanoes, and cold methane seeps.

Effects of creatine supplementation on renal function: a randomized, double-blind, placebo-controlled clinical trial

Creatine (CR) supplementation is commonly used by athletes. However, its effects on renal function remain controversial. The aim of this study was to evaluate the effects of creatine supplementation on renal function in healthy sedentary males (18-35 years old) submitted to exercise training. A randomized, double-blind, placebo-controlled trial was performed. Subjects (n = 18) were randomly allocated to receive treatment with either creatine (CR) (similar to 10 g day(-1) over 3 months) or placebo (PL) (dextrose). All subjects undertook moderate intensity aerobic training, in three 40-min sessions per week, during 3 months. Serum creatinine, serum and urinary sodium and potassium were determined at baseline and at the end of the study. Cystatin C was assessed prior to training (PRE), after 4 (POST 4) and 12 weeks (POST 12). Cystatin C levels (mg L-1) (PRE CR: 0.82 +/- 0.09; PL: 0.88 +/- 0.07 vs. POST 12 CR: 0.71 +/- 0.06; PL: 0.75 +/- 0.09, P = 0.0001) were decreased over time, suggesting an increase in glomerular filtration rate. Serum creatinine decreased with training in PL but was unchanged with training in CR. No significant differences were observed within or between groups in other parameters investigated. The decrease in cystatin C indicates that high-dose creatine supplementation over 3 months does not provoke any renal dysfunction in healthy males undergoing aerobic training. In addition, the results suggest that moderate aerobic training per se may improve renal function.

Skeletal Microstructural Abnormalities in Postmenopausal Women With Chronic Obstructive Pulmonary Disease

Chronic obstructive pulmonary disease (COPD) is associated with osteoporosis and fragility fractures. The objectives of this study were to assess static and dynamic indices of cancellous and cortical bone structure in postmenopausal women with COPD. Twenty women with COPD who had not received chronic oral glucocorticoids underwent bone biopsies after double tetracycline labeling. Biopsies were analyzed by histomorphometry and mu CT and compared with age-matched controls. Distribution of the patients according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) was: Type I (15%), Type II (40%), Type III (30%), and Type IV (15%). Mean (+/-SD) cancellous bone volume (15.20 +/- 5.91 versus 21.34 +/- 5.53%, p = .01), trabecular number (1.31 +/- 0.26 versus 1.77 +/- 0.51/mm, p = .003), and trabecular thickness (141 +/- 23 versus 174 +/- 36 mu m, p = .006) were lower in patients than in controls. Connectivity density was lower in COPD (5.56 +/- 2.78 versus 7.94 +/- 3.08 mu m, p = .04), and correlated negatively with smoking (r = -0.67; p = .0005). Trabecular separation (785 +/- 183 versus 614 +/- 136 mu m, p = .01) and cortical porosity (4.11 +/- 1.02 versus 2.32 +/- 0.94 voids/mm(2); p < .0001) were higher in COPD while cortical width (458 +/- 214 versus 762 +/- 240 mu m; p < .0001) was lower. Dynamic parameters showed significantly lower mineral apposition rate in COPD (0.56 +/- 0.16 versus 0.66 +/- 0.12 mu m/day; p = .01). Patients with more severe disease, GOLD III and IV, presented lower bone formation rate than GOLDI and II (0.028 +/- 0.009 versus 0.016 +/- 0.011 mu m(3)/mu m(2)/day;p = 04). This is the first evaluation of bone microstructure and remodeling in COPD. The skeletal abnormalities seen in cancellous and cortical bone provide an explanation for the high prevalence of vertebral fractures in this disease. (C) 2010 American Society for Bone and Mineral Research.

Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)-carbonyl complex.

We have developed a technetium labeling technology based on a new organometallic chemistry, which involves simple mixing of the novel reagent, a 99m Tc(I)-carbonyl compound, with a His-tagged recombinant protein. This method obviates the labeling of unpaired engineered cysteines, which frequently create problems in large-scale expression and storage of disulfide-containing proteins. In this study, we labeled antibody single-chain Fv fragments to high specific activities (90 mCi/mg), and the label was very stable to serum and all other challenges tested. The pharmacokinetic characteristics were indistinguishable from iodinated scFv fragments, and thus scFV fragments labeled by the new method will be suitable for biodistribution studies. This novel labeling method should be applicable not only to diagnostic imaging with 99mTc, but also to radioimmunotherapy approaches with 186/188 Re, and its use can be easily extended to almost any recombinant protein or synthetic peptide.