Relative effect of litter quality, forest type and their interaction on leaf decomposition in south-east Brazilian forests

Decomposition was studied in a reciprocal litter transplant experiment to examine the effects of forest type, litter quality and their interaction on leaf decomposition in four tropical forests in south-east Brazil. Litterbags were used to measure decomposition of leaves of one tree species from each forest type: Calophyllum brasiliense from restinga forest; Guapira opposita from Atlantic forest; Esenbeckia leiocarpa from semi-deciduous forest; and Copaifera langsdorffii from cerradao. Decomposition rates in rain forests (Atlantic and restinga) were twice as fast as those in seasonal forests (semi-deciduous and cerradao), suggesting that intensity and distribution of precipitation are important predictors of decomposition rates at regional scales. Decomposition rates varied by species, in the following order: E. leiocarpa > C. langsdorffii > G. opposita > C. brasiliense. However, there was no correlation between decomposition rates and chemical litter quality parameters: C:N, C:P, lignin concentration and lignin:N. The interaction between forest type and litter quality was positive mainly because C. langsdorffii decomposed faster than expected in its native forest. This is a potential indication of a decomposer`s adaptation to specific substrates in a tropical forest. These findings suggest that besides climate, interactions between decomposers and plants might play an essential role in decomposition processes and it must be better understood.

Effects of G a3 dosis associated with organosilicone on sweet oranges in Brazil

GA3 was tested in sweet oranges 'Pera' and 'Hamlin' for delay the picking time without loosening of fruit quality for processing. Hamlin is the firths cv processed in Brazil and Pera is a mid season cv and extending their period of processing is important. Two experiments were made at the Citrus Experimental Station during 1996 season. The treatments are 5 ppm of GA 3 + 0,05% Silwett L-77® (organosilicone), 10 ppm of GA 3 + 0,05% of Silwet L-77®, 20 ppm of GA3 + 0,1% Herbitensil® (Noniphenoloxietilate 40%m/v + isopropilic alcohol 15% m/v) and control, repeated 7 times for Hamlin and 8 times for Pera, with one tree each parcel. The treatments were applied in May 1996, at the stage of greenish yellow colour of the fruits. Evaluations were made each 20 days interval till the final picking, It was analysed fruit quality and retention force for picking and puncture resistance. The results showed no differences for fruit quality of Hamlin from July to mid September and for Pera till September. After some differences occurred. The GA3 treatments were effectives in maintain the fruit retention force for both cvs for 120 days after application. In relation to fruit puncture resistance the treatments with GA3 differed of the control for both cvs, accordingly with the doses and mixtures. The colour index was better maintained with 5ppm of GA3 plus 0,05% of Silwet L-77®. The total fruit production did not differ for both cultivars.

Avaliação de produtos comerciais de fungos entomopatogênicos no controle do psilídeo-de-concha glycaspis brimblecombei (hemiptera: psyllidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sistema filogenética da subfamília aphyocharacinae (Characiformes, Characidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Biomassa inicial do híbrido Eucalyptus urophylla vs. Eucalyptus grandis em diferentes espaçamentos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Variabilidade genética em progênies de Eucalyptus urophylla S. T. Blake para carvão vegetal

Pós-graduação em Ciência Florestal - FCA

Recomposição de reserva legal na região do tapajós: identificação de espécies e sistemas silviculturais

Esta pesquisa teve por objetivo identificar as espécies potenciais e arranjos e/ou sistemas silviculturais, que associados à vegetação espontânea, garantam características viáveis à recomposição de Reserva Legal na região do Tapajós. Foram implantados três sistemas silviculturais: 1) Plantio de árvores em canteiro de 36 plantas por espécie, em blocos ao acaso, 32 espécies que são os tratamentos, cinco repetições, espaçamento 1,5 x 1,5. 2) Plantio em One Tree Plot - OTP, em blocos ao acaso, 29 espécies como tratamentos, 12 repetições, espaçamento 3 x 3. 3) Plantio em sistema silviagrícola, onde utilizou-se o Sistema Taungya, espaçamento 7 x 7. Foi realizada a caracterização da composição florística, diversidade e similaridade da vegetação espontânea; avaliados a sobrevivência e o crescimento das espécies florestais; e produtividade da vegetação espontânea e das espécies florestais plantadas. Em toda a área estudada, foram identificados 1597 indivíduos pertencentes a 137 espécies, 98 gêneros e 40 famílias. O Índice de Shannon - Weaver encontrado foi de 3,75 para Canteiro, 3,72 para Silviagrícola e 3,56 para OTP. A similaridade pelo Índice de Sorensen encontrada foi: 65,52% entre Canteiro e OTP, 63,58% entre Canteiro e Silviagrícola e 50,31% entre OTP e Silviagrícola. Levando em consideração a taxa de sobrevivência e as médias de crescimento em DAP e altura total, a ordem decrescente de importância dos sistemas silviculturais estudados foi a seguinte: OTP 76 > OTP 75 > Canteiro 76 > Canteiro 75. Para efeitos de exploração na Área de Reserva Legal, considerando a produtividade dos indivíduos com DAP > 0,45m, a ordem de importância decrescente dos sistemas silviculturais é a seguinte: OTP > Canteiro > Silviagrícola. Como não houve diferenças estatisticamente significativas entre os Índices de Shannon – Weaver encontrados para a vegetação espontânea dos três sistemas silviculturais analisados, e levando em consideração a produtividade dos indivíduos com DAP > 0,45m, o sistema OTP seria o mais indicado para a recomposição de Reserva Legal especificamente sob o ponto de vista florestal e o sistema Silviagrícola, embora com menor produtividade florestal, poderia ser indicado à pequenos produtores por ser associado a cultivos agrícolas. Os resultados do estudo evidenciam que cerca de 40 anos após o plantio de espécies florestais nos referidos sistemas silviculturais é possível obter alta diversidade na composição florística sob estes plantios, recuperando a função ecológica e produtiva da área.

Evolutionary computation of forests with Degree- and Role-Constrained Minimum Spanning Trees

Finding the degree-constrained minimum spanning tree (DCMST) of a graph is a widely studied NP-hard problem. One of its most important applications is network design. Here we deal with a new variant of the DCMST problem, which consists of finding not only the degree- but also the role-constrained minimum spanning tree (DRCMST), i.e., we add constraints to restrict the role of the nodes in the tree to root, intermediate or leaf node. Furthermore, we do not limit the number of root nodes to one, thereby, generally, building a forest of DRCMSTs. The modeling of network design problems can benefit from the possibility of generating more than one tree and determining the role of the nodes in the network. We propose a novel permutation-based representation to encode these forests. In this new representation, one permutation simultaneously encodes all the trees to be built. We simulate a wide variety of DRCMST problems which we optimize using eight different evolutionary computation algorithms encoding individuals of the population using the proposed representation. The algorithms we use are: estimation of distribution algorithm, generational genetic algorithm, steady-state genetic algorithm, covariance matrix adaptation evolution strategy, differential evolution, elitist evolution strategy, non-elitist evolution strategy and particle swarm optimization. The best results are for the estimation of distribution algorithms and both types of genetic algorithms, although the genetic algorithms are significantly faster.

Computación evolutiva de bosques de expansión mínimos con restricción de grado y de rol

Encontrar el árbol de expansión mínimo con restricción de grado de un grafo (DCMST por sus siglas en inglés) es un problema NP-complejo ampliamente estudiado. Una de sus aplicaciones más importantes es el dise~no de redes. Aquí nosotros tratamos una nueva variante del problema DCMST, que consiste en encontrar el árbol de expansión mínimo no solo con restricciones de grado, sino también con restricciones de rol (DRCMST), es decir, a~nadimos restricciones para restringir el rol que los nodos tienen en el árbol. Estos roles pueden ser nodo raíz, nodo intermedio o nodo hoja. Por otra parte, no limitamos el número de nodos raíz a uno, por lo que, en general, construiremos bosques de DRCMSTs. El modelado en los problemas de dise~no de redes puede beneficiarse de la posibilidad de generar más de un árbol y determinar el rol de los nodos en la red. Proponemos una nueva representación basada en permutaciones para codificar los bosques de DRCMSTs. En esta nueva representación, una permutación codifica simultáneamente todos los árboles que se construirán. Nosotros simulamos una amplia variedad de problemas DRCMST que optimizamos utilizando ocho algoritmos de computación evolutiva diferentes que codifican los individuos de la población utilizando la representación propuesta. Los algoritmos que utilizamos son: algoritmo de estimación de distribuciones (EDA), algoritmo genético generacional (gGA), algoritmo genético de estado estacionario (ssGA), estrategia evolutiva basada en la matriz de covarianzas (CMAES), evolución diferencial (DE), estrategia evolutiva elitista (ElitistES), estrategia evolutiva no elitista (NonElitistES) y optimización por enjambre de partículas (PSO). Los mejores resultados fueron para el algoritmo de estimación de distribuciones utilizado y ambos tipos de algoritmos genéticos, aunque los algoritmos genéticos fueron significativamente más rápidos.---ABSTRACT---Finding the degree-constrained minimum spanning tree (DCMST) of a graph is a widely studied NP-hard problem. One of its most important applications is network design. Here we deal with a new variant of the DCMST problem, which consists of finding not only the degree- but also the role-constrained minimum spanning tree (DRCMST), i.e., we add constraints to restrict the role of the nodes in the tree to root, intermediate or leaf node. Furthermore, we do not limit the number of root nodes to one, thereby, generally, building a forest of DRCMSTs. The modeling of network design problems can benefit from the possibility of generating more than one tree and determining the role of the nodes in the network. We propose a novel permutation-based representation to encode the forest of DRCMSTs. In this new representation, one permutation simultaneously encodes all the trees to be built. We simulate a wide variety of DRCMST problems which we optimize using eight diferent evolutionary computation algorithms encoding individuals of the population using the proposed representation. The algorithms we use are: estimation of distribution algorithm (EDA), generational genetic algorithm (gGA), steady-state genetic algorithm (ssGA), covariance matrix adaptation evolution strategy (CMAES), diferential evolution (DE), elitist evolution strategy (ElististES), non-elitist evolution strategy (NonElististES) and particle swarm optimization (PSO). The best results are for the estimation of distribution algorithm and both types of genetic algorithms, although the genetic algorithms are significantly faster. iv

Congruence of fatty acid methyl ester profiles and morphological characters of arbuscular mycorrhizal fungi in Gigasporaceae.

Arbuscular mycorrhizal (AM) fungi (Order Glomales, Class Zygomycetes) are a diverse group of soil fungi that form mutualistic associations with the roots of most species of higher plants. Despite intensive study over the past 25 years, the phylogenetic relationships among AM fungi, and thus many details of evolution of the symbiosis, remain unclear. Cladistic analysis was performed on fatty acid methyl ester (FAME) profiles of 15 species in Gigaspora and Scutellospora (family Gigasporaceae) by using a restricted maximum likelihood approach of continuous character data. Results were compared to a parsimony analysis of spore morphological characters of the same species. Only one tree was generated from each character set. Morphological and developmental data suggest that species with the simplest spore types are ancestral whereas those with complicated inner wall structures are derived. Spores of those species having a complex wall structure pass through stages of development identical to the mature stages of simpler spores, suggesting a pattern of classical Haeckelian recapitulation in evolution of spore characters. Analysis of FAME profiles supported this hypothesis when Glomus leptotichum was used as the outgroup. However, when Glomus etunicatum was chosen as the outgroup, the polarity of the entire tree was reversed. Our results suggest that FAME profiles contain useful information and provide independent criteria for generating phylogenetic hypotheses in AM fungi. The maximum likelihood approach to analyzing FAME profiles also may prove useful for many other groups of organisms in which profiles are empirically shown to be stable and heritable.

Explaining the saproxylic beetle diversity of a protected Mediterranean area

Saproxylic beetle diversity is high at the Cabañeros National Park (central Spain), where woodland habitats exhibit remarkable heterogeneity. Our aim was to explain the diversity of saproxylic beetles, focusing on species turnover among mature woodland types. We surveyed five woodland types that represented the heterogeneity of the park’s woodland habitats. Beetles were collected using window traps over a period of 20 months. The Jaccard Similarity Index was used as indirect value of beta diversity among woodlands and to test the relation between species turnover and geographical distance. We also identified the contribution of species turnover to landscape diversity by using a partitioning model. Moreover, the presence of mixed woodlands (more than one tree species) allowed us to attempt to valorise the effect of tree species (coupled with their historical management) on species turnover among woodlands. Finally, we looked for different saproxylic beetle preferences for habitat and tree species using an indicator value method. We found that saproxylic beetle species composition varied significantly among the studied woodlands. The variation in species turnover was independent from the distance among woodlands, which suggested that beetle dispersal abilities could not explain this high turnover. Tree species within woodlands were a key factor that increased diversity turnover in woodlands and, consequently, the diversity of the park. Moreover, we found saproxylic beetle species that had different habitat and tree species preferences. We conclude that woodland heterogeneity (highly affected by woodland composition) seems to be the driving force for saproxylic beetle diversity in this protected area.

Coral bleaching following wintry weather

Extensive coral bleaching Occurred intertidally in early August 2003 in the Capricorn Bunker group (Wistari Reef, Heron and One Tree Islands; Southern Great Barrier Reef). The affected intertidal coral had been exposed to unusually cold (minimum = 13.3degreesC; wet bulb temperature = 9degreesC) and dry winds (44% relative humidity) for 2 d during predawn low tides. Coral bleached in the upper 10 cm of their branches and had less than 0.2 x 10(6) cell cm(-2) as compared with over 2.5 x 10(6), Cell cm(-2) in nonbleached areas. Dark-adapted quantum yields did not differ between symbionts in bleached and nonbleached areas. Exposing symbionts to light, however, led to greater quenching of Photosystem 11 in symbionts in the bleached coral. Bleached areas of the affected colonies had died by September 2003, with areas that were essentially covered by more than 80% living coral decreasing to less than 10% visible living coral cover. By January 2004, coral began to recover, principally from areas of colonies that were not exposed during low tide (i.e., from below dead, upper regions). These data highlight the importance of understanding local weather patterns as well as the effects of longer term trends in global climate.

Habitat Map for Lizard Island reef, Australia derived from a photo-transect survey field data collected in December 2011 and September/October 2012

An object based image analysis approach (OBIA) was used to create a habitat map of the Lizard Reef. Briefly, georeferenced dive and snorkel photo-transect surveys were conducted at different locations surrounding Lizard Island, Australia. For the surveys, a snorkeler or diver swam over the bottom at a depth of 1-2m in the lagoon, One Tree Beach and Research Station areas, and 7m depth in Watson's Bay, while taking photos of the benthos at a set height using a standard digital camera and towing a surface float GPS which was logging its track every five seconds. The camera lens provided a 1.0 m x 1.0 m footprint, at 0.5 m height above the benthos. Horizontal distance between photos was estimated by fin kicks, and corresponded to a surface distance of approximately 2.0 - 4.0 m. Approximation of coordinates of each benthic photo was done based on the photo timestamp and GPS coordinate time stamp, using GPS Photo Link Software (www.geospatialexperts.com). Coordinates of each photo were interpolated by finding the gps coordinates that were logged at a set time before and after the photo was captured. Dominant benthic or substrate cover type was assigned to each photo by placing 24 points random over each image using the Coral Point Count excel program (Kohler and Gill, 2006). Each point was then assigned a dominant cover type using a benthic cover type classification scheme containing nine first-level categories - seagrass high (>=70%), seagrass moderate (40-70%), seagrass low (<= 30%), coral, reef matrix, algae, rubble, rock and sand. Benthic cover composition summaries of each photo were generated automatically in CPCe. The resulting benthic cover data for each photo was linked to GPS coordinates, saved as an ArcMap point shapefile, and projected to Universal Transverse Mercator WGS84 Zone 56 South. The OBIA class assignment followed a hierarchical assignment based on membership rules with levels for "reef", "geomorphic zone" and "benthic community" (above).

Constructal Design of Energy Systems

This dissertation shows the use of Constructal law to find the relation between the morphing of the system configuration and the improvements in the global performance of the complex flow system. It shows that the better features of both flow and heat transfer architecture can be found and predicted by using the constructal law in energy systems. Chapter 2 shows the effect of flow configuration on the heat transfer performance of a spiral shaped pipe embedded in a cylindrical conducting volume. Several configurations were considered. The optimal spacings between the spiral turns and spire planes exist, such that the volumetric heat transfer rate is maximal. The optimized features of the heat transfer architecture are robust. Chapter 3 shows the heat transfer performance of a helically shaped pipe embedded in a cylindrical conducting volume. It shows that the optimized features of the heat transfer architecture are robust with respect to changes in several physical parameters. Chapter 4 reports analytically the formulas for effective permeability in several configurations of fissured systems, using the closed-form description of tree networks designed to provide flow access. The permeability formulas do not vary much from one tree design to the next, suggesting that similar formulas may apply to naturally fissured porous media with unknown precise details, which occur in natural reservoirs. Chapter 5 illustrates a counterflow heat exchanger consists of two plenums with a core. The results show that the overall flow and thermal resistance are lowest when the core is absent. Overall, the constructal design governs the evolution of flow configuration in nature and energy systems.

Defensive role of allelopathic secondary compounds in plants I: testing two independent general hypotheses

This study tests two general and independent hypotheses with the basic assumption that phytoactive secondary compounds produced by plants evolved primarily as plant defences against competitor plant species. The first hypothesis is that the production and main way of release of phytoactive compounds reflect an adaptive response to climatic conditions. Thus, higher phytoactivity by volatile compounds prevails in plants of hot, dry environments, whereas higher phytoactivity by water-soluble compounds is preponderant in plants from wetter environments. The second hypothesis is that synergy between plant phytoactive compounds is widespread, due to the resulting higher energy efficiency and economy of resources. The first hypothesis was tested on germination and early growth of cucumber treated with either water extracts or volatiles from leaves or vegetative shoot tops of four Mediterranean-type shrubs. The second hypothesis was tested on germination of subterranean clover treated with either water extracts of leaves or vegetative shoot tops of one tree and of three Mediterranean-type shrubs or with each of the three fractions obtained from water extracts. Our data do not support either hypotheses. We found no evidence for higher phytoactivity in volatile compounds released by plants that thrive in hot, dry Mediterranean-type environments. We also found no evidence for the predominance of synergy among the constituents of fractions. To the contrary, we found either antagonism or no interaction of effects among allelopathic compounds.