Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Silk fibroin has been widely employed in various forms as biomaterials for biomedical applications due to its superb biocompatibility and tunable degradation and mechanical properties. Herein, silk fibroin microparticles of non-mulberry silkworm species (Antheraea assamensis, Antheraea mylitta and Philosamia ricini) were fabricated via a top-down approach using a combination of wet-milling and spray drying techniques. Microparticles of mulberry silkworm (Bombyx mori) were also utilized for comparative studies. The fabricated microparticles were physico-chemically characterized for size, stability, morphology, chemical composition and thermal properties. The silk fibroin microparticles of all species were porous (∼5μm in size) and showed nearly spherical morphology with rough surface as revealed from dynamic light scattering and microscopic studies. Non-mulberry silk microparticles maintained the typical silk-II structure with β-sheet secondary conformation with higher thermal stability. Additionally, non-mulberry silk fibroin microparticles supported enhanced cell adhesion, spreading and viability of mouse fibroblasts than mulberry silk fibroin microparticles (p<0.001) as evidenced from fluorescence microscopy and cytotoxicity studies. Furthermore, in vitro drug release from the microparticles showed a significantly sustained release over 3 weeks. Taken together, this study demonstrates promising attributes of non-mulberry silk fibroin microparticles as a potential drug delivery vehicle/micro carrier for diverse biomedical applications.

Microbiological control of moisturizing mask formulation added of hibiscus flowers, assai palm, black mulberry and papaw glycolic extracts

The microbiological control of moisturizing mask formulation added of hibiscus flowers, assai palm, black mulberry and papaw glycolic extracts, determining the number of viable microorganisms and possible presence of pathogenic. The moisturizing mask formulation was composed of zinc oxide (5. 0%) and moisturizing cream constituted of triceteareth-4 phosphate (and) cetyl alcohol (and) stearyl alcohol (and) sodium cetearyl sulfate (and) oleth-10 (qs 50g). To this formulation was added hibiscus flowers glycolic extract (2. 5%), assai palm glycolic extract (1. 5%), black mulberry glycolic extract (1. 5%) and papaw glycolic extract (2. 0%). The formulation was stored in aseptically clean recipients, away from humidity and light, in fresh and airy places. The results of the microbiological analysis on the counting of aerobic mesophilic microorganisms (bacteria and fungi), of the above mentioned formulation, revealed a bioburden < 10 CFU/mL in all samples. Such data indicate adequate microbiological quality of the tested products, according to official recommendations. Furthermore, it was not detected the presence of pathogenic microorganisms, assuring the harmlessness of the formulation. The results lead us to conclude that the formulation and raw materials analyzed did not present microbial contamination, evidenced for estimating the number of viable microorganisms (<10 UFC/g) and for researching pathogens.

A methodical treatise on the cultivation of the mulberry tree, on the raising of silk worms, and on winding the silk from the cocoons. Abridged from the French of M. de La Brousse, with notes and an appendix.

"Errata," inserted.

Summary of the principal Chinese treaties upon the culture of the mulberry and the rearing of silk worms.

"This S̀ummary' was first translated from the Chinese, by Stanislas Julien ... and printed at the Royal Press, in Paris. The French copy from which this translation was made, was transmitted from Paris to the Secretary of State, and by his recommendation has been translated and published here. Washington, February, 1838."--Publisher's note.

Hints on the cultivation of the mulberry, with some general observations on the production of silk.

Mode of access: Internet.

The silk culture in the United States; embracing complete accounts of the latest and most approved modes of hatching, rearing and feeding the silk-worm, managing a cocoonery, reeling, spinning, and manufacturing the silk, &c. ... With brief historical sketches of the silk business, natural history of the silk-worm, the mulberry, &c. comp. from the most approved and reliable works ... To which is added Blydenburgh's Manual of the silk culture; statistics of silk imports, &c., &c.

"The work ... has been prepared ... with the aid of Mr. I. R. Barbour ... we have ... profited by the article on silk in Dr. Ures ... dictionary of arts and sciences, Mr. Bliss's report on silk ... to the legislature of Ohio, Mr. Colman's report to the Massachusetts legislature, &c.; while we have quoted entire The silk manual of ... Mr. Blydenburgh." Advertisement, p. [3]

A methodical treatise on the cultivation of the mulberry tree, on the raising of silk worms, and on winding the silk from the cocoons.

Mode of access: Internet.

The American silk growers guide; or, The art of raising the mulberry and silk, and the system of successive crops in each season.

Goldsmiths'-Kress no. 30853.9.

A manual containing directions for sowing, transplanting and raising of the mulberry tree : together with proper instructions for propagating the same by cuttings, layers, &c., &c. As also, directions for the culture of silk: to which is added, calculations shewing the produce and probable expense of cultivation of from one to ten acres, as tested by actual results /

Checklist Amer. imprints,

An oration, delivered on the 49th anniversary of American independence, in the Mulberry Street Church before the hibernian, tailors, cordwainers, shipwrights, stone cutters, bakers and coopers' societies.

Mode of access: Internet.

Resistance of three different populations of mulberry silkworm (Bombyx mori L.) to Bacillus thuringiensis

The relative resistance levels of three different populations of mulberry silkworm, Bombyx mori L. to Bacillus thuringiensis (Bt) has been studied. All three populations (two Australian and one Indonesian) were observed for similar characteristics including 3rd instar larval mortality at 24, 48, 72 and 96 h after treatment (HAT), LD50 ratio and probit mortality. Among the Australian two populations, the QuBill (yellow coloured, oval shaped cocoon) population showed higher larval mortality to Bt toxicity compared to the QuBite (white coloured, oval shaped cocoon) population. When all the populations were compared, the Insab (Indonesian population with white Coloured, peanut shaped cocoon) showed lower larval mortality and highest LD50 ratio up to 48 HAT. The Insab population also showed a 24 It longer incubation/latent period prior to the start Of Mortality.

Italian-American Civil Rights League booth at the Fiesta of Maria S.S. della Grazie on Mulberry Street

General note: Title provided by Freda Leinwand.

Italian-American Civil Rights League sign at the Fiesta of Maria S.S. della Grazie on Mulberry Street

Inscriptions: Verso: [stamped] Photograph by Freda Leinwand. [463 West Street, Studio 229G, New York, NY 10014].