Escala macroscópica de maturaçāo e ciclo sexual das espécies Penaeus indicus H. Milne Edward e Metapenaeus monoceros (Fabricius)

The macroscopic scale used for determination of sexual maturity in shrimps was tested against objective parameters namely the gonad-somatic index and the size of oocytes. The two main species caught in Mozambique, Penaeus indicus and Metapenaeus monoceros, were the object of the work. In order to reduce the subjectivity in the macroscopic exam, a table with the colours representative of each stage is presented. Since this study was conducted over a period of 13 consecutive months and there were observations from previous years, the sexual cycle of the two species is also presented, as well as an estimate of the size at first maturity.

Mortality rates estimates for Metapenaeus monoceros (Fabricius) of Maputo Bay

The species Metapenaeus monoceros in Maputo Bay recruits to the fishery almost all over the year, but the main recruitment occurs over a short period of time, each year, during April-May. Growth curves were constructed by following the progression of modes over a period of time. The von Bertalanffy growth parameters were estimated as L∞=31.9 mm and K(monthly)=0.25, for males, and L∞=48.3 mm and K(monthly)=0.14, for females (Brinca and Sousa, 1984). Using data on growth and length composition of the catches, the following methods were applied to estimate mortality rates of M. monoceros: estimation of natural mortality by using approximative methods; use of c.p.u.e. data; catch curves; cohort analysis; average age and length methods.

A study on growth of Metapenaeus monoceros (Fabricius) of Maputo Bay

Biological data on Metapenaeus monoceros has been regularly collected in Maputo Bay since 1968. The main objective of this report is to study growth as a function of the size, since this is one of the basic parameters for population dynamics. As the possibility of studying shrimp growth by modal progression analysis depends very much on the space-time configuration of sampling, the years 1968 and 1969 were chosen to study growth of the population available to the bottom trawl used by the fishing industry. In those years sampling was very frequent (twice a week) and the samples were collected from a rather small fishing area. Complementary data on the spawning, juvenile phase and recruitment to the fishery was used to establish the relationship between the different stages of the life cycle and to obtain an age/length key. Data on juveniles in estuaries was only available for 1969 and 1973.

Selection of bacterial flora in the Chlortetracycline treated oil sardine (Sardinella longiceps), Indian mackerel (Rastrelliger kanagurta) and prawn (Metapenaeus dobsoni) during ice storage

The native flora of oil sardine and mackerel consisting of Pseudomonas spp; Moraxella spp., Acinetobacter spp. and Vibrio spp. underwent significant changes during ice storage. At the time of spoilage, Pseudomonas spp. were predominant. CTC treatment significantly reduced the Pseudomonas spp. in the initial stages of storage; but later Pseudomonas spp. reasserted and constituted the bulk of the spoilage flora. In prawn, the native flora was comprised of Pseudomonas spp., Acinetobacter spp., Moraxella spp. and Vibrio spp. At the time of spoilage a heterogeneous flora, consisting of Pseudomonas spp; Moraxella spp. and Acinetobacter spp. predominated. CTC treatment significantly changed the flora of prawns. During spoilage, Pseudomonas predominated in CTC treated prawns.

Bacterial flora of EDTA treated oil sardine (Sardinella longiceps), Indian mackerel (Rastrelliger kanagurta) and prawn (Metapenaeus dobsoni) in ice storage

The native flora of fresh oil sardine and mackerel consisted mainly of Pseudomonas spp., Moraxella spp., Acinetobacter spp. and Vibrio spp. During spoilage in ice, nearly 75% of their bacterial flora belonged to Pseudomonas spp. alone. But Na sub(2) EDTA treatment reduced the proportion of Pseudomonas spp. considerably and the major bacterial groups at the time of spoilage were Moraxella spp. and Acinetobacter spp. In the case of fresh prawn, the native flora was constituted by Pseudomonas spp., Moraxella spp., Acinetobacter spp. and Vibrio spp. At the time of spoilage of prawn in ice, Moraxella spp. and Acinetobacter spp. predominated, together constituting 74% of the total population. Na sub(2) EDTA treatment did not alter significantly the spoilage flora of prawns. Moraxella spp. and Acinetobacter spp. accounted for 86% of the spoilage flora in ice storage of Na sub(2) EDTA treated prawns.

Effectiveness of EDTA dips on the shelf-life of oil sardine (Sardinella longiceps), mackerel (Rastrelliger kanagurta) and prawn (Metapenaeus dobsoni) in iced storage

Fresh oil sardine, mackerel and prawn were dipped in 0.1% and 1% solutions of Na sub(2)EDTA, and stored in ice. Their storage-life was assessed by bacteriological, chemical and sensory methods. Even though EDTA treatment controlled the increase in bacterial counts and reduced TMA and TVBN production in oil sardine and mackerel, the consequent beneficial effect was not realised because of the deterioration of fat in these fishes, leading to rancidity. But, for prawn stored in ice, a dip in 1% solution of Na sub(2)EDTA enhanced the shelf-life by at least 8 days over the untreated control. EDTA absorbed by the muscle of fish and prawn during dip in Na sub(2)EDTA solution is not completely removed during their iced storage for 25 days.

A quantitative study of Vibrio parahaemolyticus (Sakazaki et al) in Etroplus suratensis (Bloch) and Metapenaeus dobsoni (Miers) from Cochin backwater

Seasonal variation of Vibrio parahaemolyticus in fish (Etroplus sauratensis) and prawn (Metapenaeus dobsoni) was monitored from March 1982 to February 1983. Analyses of total viable count, vibrio-like organisms, V. parahaemolyticus like organisms and V. parahaemolyticus showed that they occur more in prawn than in fish. In a more polluted environment, the counts of V. parahaemolyticus associated with fish were found to be higher than in prawn.

INVESTIGATIONS ON THE STRESS EFFECTS OF PETROLEUM HYDROCARBONS ON METAPENAEUS DOBSON (MIERS)

The present investigation has addressed the effects of PHC contaminated culture medium on the morphology, physiology and behaviour of shrimps. The shrimp Metapenaeus dobsoni is an important member of the crustacean animal community abounding the oil contaminated benthic regions of Cochin backwater system. Since it is known that true pollutants can disrupt the sustainability of ecosystems by its effect on species, populations and communities,a representative species was used for the study. The results discussed in this work is bound to help in understanding the ecotoxicant resistance that the animal may display under toxic conditions compared to dynamic steady-state systems in nature.

Biology, population characteristics and fishery of the speckled shrimp Metapenaeus monoceros (Fabricius, 1798) along the Kerala coast

Crustaceans comprising numerous edible species of prawns, lobsters and crabs inhabiting different ecosystem form significant portion of the aquatic food resources of the world. Among the crustaceans, prawns are the most commercially exploited group and hold premier rank by virtue of their importance as an esteemed food of gourmet and on account of their high export value. Met-ape-naeus manoceras (Fabricius, 1798) which is known IS,Speckled shrimp’ (FAD name) and ‘Brown shrimp’ ( common nameused in the industry) is one of the commercially important marine penaeid prawns of India. During 1995, M. monaceros catch constituted 7.5 Z of the all India marine penaeid prawn landings. M. monoceros attains a maximum length of about 200 mm and has high export potential.Thus realising the growing importance of M. monoceros in the capture fisheries, it was felt, that it would be ideal to carry out detailed study on this species for rational exploitation and management of its fishery. Hence, the present work entitled, “Biology, population characteristics and fishery of the speckled shrimp Hetapenaeus monoceros (Fabricius, 1798) along Kerala coast“ was undertaken by the author. The thesis is laid out in seven chapters comprising TAXONOMY, FOOD AND FEEDING HABITS, AGE AND GROWTH, REPRODUCTION,LENGTH-WEIGHT RELATIONSHIP, FISHERY and POPULATION DYNAMICS

Studies on osmoregulation in the penaeid prawn Metapenaeus dobsoni (Miers)

Metapenaeus dobsoni (Miers) is the most abundant species along the coast of Kerala. It is cultured extensively by adoption of traditional farming practices. The geographical location and water source determines the seasonal and annual environmental fluctuations the prawn farming systems experiences. The life cycle of the shrimp includes its migration to the coastal deeper waters for spawning and the immigration of larvae to the estuaries for growth. The survival of the species in such complex ecosystems is thus critical to its life cycle. The animal adapts itself to different environments through a physiological process known as osmoregulation. The present study on osmoregulation in the penaeid prawn Metapenaeus dobsoni was thus undertaken to understand the mechanism adopted by this species to survive in different environments. A number of experimental work have been conducted to understand the effect of salinity on the internal variations. However the effect of the complex environmental conditions as existent in nature on the osmotic variations in this species has not been dealt with in any of the earlier studies.

Investigations on the stress effects of petroleum hydrocarbons on Metapenaeus dobsoni (Miers)

The present investigation has addressed the eflects of PHC contaminated culture medium on the morphology, physiology and behaviour of shrimps] The shrimp Metapenaeus dobsoni is an important member of the crustacean animal community abounding the oil contaminated benthic regions of Cochin backwater system. Since it is known that true pollutants can disrupt the sustainability of ecosystems by its eflect on species, populations and communities, a representative species was used for the study. The results discussed in this work is bound to help in understanding the ecotoxicant resistance that the animal may display under toxic conditions compared to aynamic steaay-state systems in nature

Spoilage pattern of five species of Australian prawns: Deterioration is influenced by environment of capture and mode of storage

Five species of commercial prawns Penaeus plebejus, P. merguiensis, P. semisulcatus/P. esculentus and M. bennettae, were obtained from South-East and North Queensland, chilled soon after capture and then stored either whole or deheaded on ice and ice slurry, until spoilage. Total bacterial counts, total volatile nitrogen, K-values and total demerit scores were assessed at regular intervals. Their shelf lives ranged from 10-17 days on ice and >20 days on ice slurry. Initial bacterial flora on prawns from shallower waters (4-15m) were dominated by Gram-positives and had lag periods around 7 days, whereas prawns from deeper waters (100m) were dominant in Pseudomonas spp. with no lag periods in bacterial growth. The dominant spoiler in ice was mainly Pseudomonas fragi whereas the main spoiler in ice slurry was Shewanella putrefaciens. Bacterial interactions seem to play a major role in the patterns of spoilage in relation to capture environment and pattern of storage

Raw data for project "Development of a DNA based aging technique for use in fisheries assessments" Fisheries and Development Corporation project 2007/033

The primary aim of this study was to determine the relationship between telomere length and age in a range of marine invertebrates including abalone (Haliotis spp) oysters (Saccostrea glomerata), spiny lobsters (Sagmariasus verreauxi formerly Jasus verreauxi and Jasus edwardsii) and school prawns (Metapenaeus macleayi). Additionally, this relationship was studied in a vertebrate organism using the freshwater fish Silver perch (Bidyanus bidyanus). Telomere length differences between tissues were also examined in some species such as Saccostrea glomerata, Sagmariasus verreauxi and Bidyanus bidyanus. In some cases cultured specimens of known age were used and this is quoted in the spreadsheets. For other wild-caught specimens where age was not known, size was used as a proxy for age. This may be a broad size class, or be determined by shell size or carapace length depending on the organism. Each spreadsheet contains raw data of telomere length estimates from Terminal Restriction Fragment Assays (TRF) for various individuals of each species including appropriate details such as age or size and tissue. Telomere length estimates are given in base pairs (bp). In most cases replicate experiments were conducted on groups of samples three times but on a small number of occasions only two replicate experiments were conducted. Further description of the samples can be found in final report of FRDC 2007/033. The arithmetic average for each individual (sample ID) across the two or three replicate experiments is also given. Bidyanus bidyanus (SilverPerch) Two sheets are contained within. a) Comparison of telomere length between different tissues (heart, liver and muscle) within the three year old age class - two replicate experiments were conducted. b) Comparison of telomere length between fish of different but known ages (0.25, 1, 2, and 3 years old) in each of three tissues, heart, liver and muscle – three replicate experiments were conducted per tissue. Haliotis spp (Abalone species) Three species were tested. H. asinina Telomere length was compared in two age classes-11 month and 18 month old abalone using muscle tissue from the foot. Within gel-variation was also estimated using a single sample run three times on one gel (replicate experiment). H. laevigata x H. rubra hybrids Telomere length was compared in three known age classes – two, three and four years old using muscle tissue from the foot. H. rubra Telomere length was compared in a range of different sized abalone using muscle tissue from the foot. Shell size is also given for each abalone Saccostrea glomerata Three sheets are contained within the file. a) Samples came from Moreton Bay Queensland in 2007. Telomere length was compared in two tissues (gill and mantle) of oysters in three age groups (1, 3 and 4 years) b) Samples came from Moreton Bay Queensland in 2009. Telomere length was compared in three age classes using DNA from gill tissue only c) Samples came from Wallis Lake, New South Wales. Telomere length was estimated from whole body minus the shell from 1 year old oysters, gill tissue of 3 age classes (1.5 years, 3 and 4 years), mantle tissue of two age classes (3 and 4 years). Sagmariasus verreauxi (formerly Jasus verreauxi) Telomere length was estimated from abdomen tissue of puerulus, gill and muscle tissue of 3 year old, large and very large size classes of lobsters. Jasus edwardsii Telomere length was measured in two size classes of lobsters- adults of varying sizes using muscle tissue and puerulus using tissues from the abdomen minus the exoskeleton. Metapenaeus macleayi Telomere length was measured in three size classes of school prawns adults. Muscle tissue was used, minus the exoskeleton.

Development of a DNA based aging technique for use in fisheries assessments. Final report, Australian Fisheries Research and Development Corporation Project 2007/033.

The productivity of a fisheries resource can be quantified from estimates of recruitment, individual growth and natural and fisheries-related mortality, assuming the spatial extent of the resource has been quantified and there is minimal immigration or emigration. The sustainability of a fisheries resource is facilitated by management controls such as minimum and maximum size limits and total allowable catch. Minimum size limits are often set to allow individuals the opportunity to reproduce at least once before the chance of capture. Total allowable catches are a proportion of the population biomass, which is estimated based on known reproduction, recruitment, mortality and growth rates. In some fisheries, however, management actions are put in place without quantification of the resource through the stock assessment process. This occurs because species-specific information, for example individual growth, may not be available. In these circumstances, management actions need to be precautionary to protect against future resource collapse, but this often means that the resource is lightly exploited. Consequently, the productivity of the resource is not fully realised. Australia’s most valuable fisheries are invertebrate fisheries (Australian Department of Agriculture Fisheries and Forestry, 2008). For example, Australian fisheries (i.e. excluding aquaculture) production of crustaceans (largely prawns, rock lobster and crab) was 41,000 tonnes in 2006/7, worth $778 million. Production from mollusc (largely abalone, scallops, oysters and squid) fisheries was 39,000 tonnes, worth $502 million. Together, in 2006/7 crustacean and mollusc fisheries represented 58% of the total value of Australian wild fisheries production. Sustainable management of Australia’s invertebrate fisheries is frustrated by the lack of data on species-specific growth rates. This project investigated a new method to estimate age, and hence individual growth rates, in invertebrate fisheries species. The principle behind the new aging method was that telomeres (i.e. DNA end-caps of chromosomes) get shorter as an individual gets older. We studied commercial crustacean and molluscan species. A vertebrate fish species (silver perch, Bidyanus bidyanus) was used as a control to standardise our work against the literature. We found a clear relationship between telomere length and shell size for temperate abalone (Haliotis rubra). Further research is recommended before the method can be implemented to assist management of wildharvested abalone populations. Age needs to be substituted for shell size in the relationship and it needs to be studied for abalone from several regions. This project showed that telomere length declined with increasing age in Sydney rock oysters (Saccostrea glomerata) and was affected by regional variation. A relationship was not apparent between telomere length and age (or size as a surrogate for age) for crustacean species (school prawns, Metapenaeus macleayi; eastern rock lobster, Sagmariasus verreauxi; southern rock lobster, Jasus edwardsii; and spanner crabs, Ranina ranina). For school prawns, there was no difference between telomere length in males and females. Further research is recommended, however, as telomeric DNA from crustaceans was difficult to analyse using the terminal restriction fragment (TRF) assay. Telomere lengths of spanner crabs and lobsters were at the upper limit of resolution of the assay used and results were affected by degradation and possible contamination of telomeric DNA. It is possible that telomere length is an indicator of remaining lifespan in molluscan and crustacean individuals, as suggested for some vertebrate species (e.g. Monaghan, 2010). Among abalone of similar shell size and among lobster pueruli, there was evidence of individuals having significantly longer or shorter telomeres than the group average. At a population level, this may be a surrogate for estimates of future natural mortality, which may have usefulness in the management of those populations. The method used to assay telomere length (terminal restriction fragment assay) performed adequately for most species, but it was too expensive and time-consuming to be considered a useful tool for gathering information for fisheries management. Research on alternative methods is strongly recommended.

Estimativa da captura total de peixe e camarão das pescarias de emalhe e de arrasto da Baía de Maputo, em 1985

The most important fisheries of Maputo Bay (Mozambique) are the gill netting of the Hilsa kelee and the trawling of the shrimps Penaeus indicus and Metapenaeus monoceros . Data of capture and yields are presented and the number of artisanal fishing boats and semi-industrial fishing vessels is given.