POU domain transcription factor Brn3b is critical for the normal development and survival of retinal ganglion cells

The POU domain transcription factor Brn3b/POU4F2 plays a critical role regulating gene expression in mouse retinal ganglion cells (RGCs). Previous investigations have shown that Brn3b is not required for initial cell fate specification or migration; however, it is essential for normal RGC differentiation. In contrast to wild type axons, the mutant neurites were phenotypically different: shorter, rougher, disorganized, and poorly fasciculated. Wild type axons stained intensely with axon specific marker tau-1, while mutant projections were weakly stained and the mutant projections showed strong labeling with dendrite specific marker MAP2. Brn-3b mutant axonal projections contained more microtubules and fewer neurofilaments, a dendritic characteristic, than the wild type. The mutant neurites also exhibited significantly weaker staining of neurofilament low-molecular-weight (NF-L) in the axon when compared to the wild type, and NF-L accumulation in the neuron cell body. The absence of Brn-3b results in an inability to form normal axons and enhanced apoptosis in RGCs, suggesting that Brn-3b may control a set of genes involved in axon formation. ^ Brn3b contains several distinct sequence motifs: a glycine/serine rich region, two histidine rich regions, and a fifteen amino acid conserved sequence shared by all Brn3 family members in the N-terminus and a POU specific and POU homeodomain in the C-terminus. Brn3b activates a Luciferase reporter over 25 fold in cell culture when binding to native brn3 binding sites upstream of a minimal promoter. When fused to the Gal4 DNA Binding domain (DBD) and driven by either a strong (CMV) or weaker (pAHD) promoter, the N-terminal of Brn3b is capable of similar activation when binding to Gal4 UAS sites, indicating a presumptive activator of transcription. Both full length Brn3b or the C-terminus fused to the Gal4DBD and driven by pCMV repressed a Luciferase reporter downstream of UAS binding sites. Lower levels of expression of the fusion protein driven by pADH resulted in an alleviation of repression. This repression appears to be a limitation of this system of transcriptional analysis and a potential pitfall in conventional pCMV based transfection assays. ^

Houston police officer attitudes towards the mentally ill: CIT trained and non-CIT trained

The purpose of this study was to develop a better understanding of police officer attitudes towards the mentally ill and what impact that might have on their behavior. Focused on the effects of Crisis Intervention Team (CIT) training on Houston police officers, this research wanted to determine if CIT training decreases attitudes of authoritarianism and increases attitudes of self-efficacy in dealing with the mentally ill—other factors assessed were age, years of service, ethnicity, and gender. Results confirmed that CIT training had an effect on an officer's attitudes with CIT officers being less authoritarian and having more self-efficacy with respect to dealing with the mentally ill as compared to non-CIT officers. Because of these results, this study could offer support in tailoring training programs to have successful officer-mentally ill person interactions. ^

Architecture,

Bibliographical footnotes.

The Pacific railroads : suggestions submitted to the Hon. Charles Devens, attorney-general of the United States, on the resolutions of the Senate and House of Representatives in respect to the operation of the Union Pacific Railroad and branches : on behalf of said railroad company /

Mode of access: Internet.

Essays in biography and criticism.

Volumes paged separately.

Our Campbell ancestors, 1742-1937. Traditions and history of the family of five Campbell brothers and sisters; John, James Douglas, Hester, Mary and Samuel: including what is known of them in New Jersey; York county, Pennsylvania; Union county, South Carolina and in Ohio. A genealogy of the known descendants of John Campbell through his son James, and Samuel Campbell through his son Ralph, also brief ancestral notes on families connected by marriage with the foregoing; viz; Parnell, Clark, Spray, Wilson, Haskett, Mendenhall and Underhill,

Bibliographical foot-notes.

Gerbert, Beiträge zur Kenntnis der Mathematik des Mittelalters.

Mode of access: Internet.

Die Principien der höheren Analysis in ihrer Entwicklung von Liebniz bis auf Lagrange, als ein historisch-kritischer Beitrag zur Geschichte der Mathematik dargestellt,

Mode of access: Internet.

Appréciation du règne de Charles le Téméraire et des projets conçus par ce prince dans l'intéêt de la maison de Bourgogne ...

"Extrait du tome XXIV des Mémoires couronnés et autres mémoires publiés par l'Académie royale de Belgique. 1874."

Leighton.

At head of title: Masters in art; a series of illustrated monographs issued monthly.

Frederic Lord Leighton : late president of the Royal Academy of Arts : an illustrated record of his life and work /

Includes index.

Exhibition of drawings in water colour by Alfred William Hunt ...

Biographical sketch of Hunt by Cosmo Monkhouse.

The life, letters and work of Frederic Leighton,

Plates, with the exception of the photogravures, mounted.

A short account of the first preaching of the gospel by the apostles : being a continuation of "conversations on the life of Jesus Christ" by a mother.

Mode of access: Internet.

Essai comparatif entre le cardinal-duc de Richelieu, premier ministre de Louis XIII, roi de France, et M. William Pitt, premier ministre de George III, roi de la Grande-Bretagne /

Mode of access: Internet.