Vaginal delivery of the recombinant HIV-1 clade-C trimeric gp140 envelope protein CN54gp140 within novel rheologically structured vehicles elicits specific immune responses

Rheologically structured vehicle (RSV) gels were developed as delivery systems for vaginal mucosal vaccination with an HIV-1 envelope glycoprotein (CN54gp140). RSVs comprised a mucoadhesive matrix forming and vaginal fluid absorbing polymer. The mucoadhesive and rheological properties of the RSVs were evaluated in vitro, and the distribution, antigenicity and release of CN54gp140 were analysed by ELISA. CN54gp140 was uniformly distributed within the RSVs and continuously released in vitro in an antigenically intact form over 24 h. Vaginal administration to rabbits induced specific serum IgG, and IgG and IgA in genital tract secretions. The RSVs are a viable delivery modality for vaginal immunization.

Eva Heinitz and Malcolm Hamilton, Recital for Viola da Gamba and Harpsichord, January 27, 1966

Concert Program for Eva Heinitz and Malcolm Hamilton, Recital for Viola da Gamba and Harpsichord, January 27, 1966

Bond between Alexander Fraser of the Township of Dumphries to the Honourable William Dickson of Galt

Bond (1 page, printed) between Alexander Fraser of the Township of Dumphries to the Honourable William Dickson of Galt for 304 pounds, June 21, 1830.

Province of Upper Canada Grant (vellum) to Thomas Fraser of the Township of Edwardsburgh granted 1 acre in Lot no.9 in the County of Stormont

Province of Upper Canada Grant (vellum) to Thomas Fraser of the Township of Edwardsburgh granted 1 acre in Lot no.9 in the County of Stormont. Signed by William Jarvis, Sir Isaac Brock, Prideaux Selby and John Macdonell. There are some holes in the document and there are small pieces missing on the right hand side. William Jarvis was the Provincial Secretary of the Lt. Governor of Upper Canada; Sir Isaac Brock. Jarvis was an officer in the Queen’s Rangers. He also served as Provincial Secretary of Upper Canada. A partial crown seal is attached, Mar. 26, 1812.