RAIN EVENTS INFLUENCE SHORT-TERM FEEDING PREFERENCES IN THE SNAIL CEPAEA NEMORALIS

Terrestrial gastropods are both herbivores and detritivores, but the ratio between these two modes of feeding can be highly variable over time. While previous studies have examined long-term seasonal patterns in the consumption of fresh material, mechanisms explaining short-term variation in dietary preferences have not been explored. We used faecal analysis to determine how short-term variation in weather affects the ratio of herbivory to detritivory in the land snail Cepaea nemoralis. Averaged across sampling dates, c. 9% of the faeces were composed of fresh plant material, with the remainder consisting of plant litter and soil. Temperature, relative humidity and soil moisture did not affect the proportional consumption of fresh material; however, snails consumed more soil with increasing temperature. If there had not been a recent precipitation event, the mean proportion of fresh material in the faeces more than doubled on average; however, this increase only occurred in areas of low herbaceous cover. Our results suggest that an increased proportion of snails consume fresh material during dry periods to compensate for water losses. Moreover, our study highlights that studies of dietary composition in the field need to account for short-term variation in feeding
preferences caused by weather.

NEUROPATHOPHYSIOLOGICAL MECHANISMS IN GLUTARIC ACIDURIA TYPE I: 3-HYDROXYGLUTARIC ACID LEADS TO AMMONIA INCREASE AND NON-APOPTOTIC CELL DEATH

A 3D in vitro model of rat organotypic brain cell cultures in aggregates was used to investigate neurotoxicity mechanisms in glutaric aciduria type I (GA-I). 1 mM glutarate (GA) or 3-hydroxyglutarate (3OHGA) were repeatedly added to the culture media at two different time points. In cultures treated with 3OHGA, we observed an increase in lactate in the medium, pointing to a possible inhibition of Krebs cycle and respiratory chain. We further observed that 3OHGA and to a lesser extend GA induced an increase in ammonia production with concomitant decrease of glutamine concentrations, which may suggest an inhibition of the astrocytic enzyme glutamine synthetase. These previously unreported findings may uncover a pathogenic mechanism in this disease which has deleterious effects on early stages of brain development. By immunohistochemistry we showed that 3OHGA increased non-apoptotic cell death. On the cellular level, 3OHGA and to a lesser extend GA led to cell swelling and loss of astrocytic fibers whereas a loss of oligodendrocytes was only observed for 3OHGA. We conclude that 3OHGAwas the most toxic metabolite in our model for GA-I. 3OHGA induced deleterious effects on glial cells, an increase of ammonia production, and resulted in accentuated cell death of non-apoptotic origin.

Impact of a cis-associated gene expression SNP on chromosome 20q11.22 on bipolar disorder susceptibility, hippocampal structure and cognitive performance.

BackgroundBipolar disorder is a highly heritable polygenic disorder. Recent enrichment analyses suggest that there may be true risk variants for bipolar disorder in the expression quantitative trait loci (eQTL) in the brain.AimsWe sought to assess the impact of eQTL variants on bipolar disorder risk by combining data from both bipolar disorder genome-wide association studies (GWAS) and brain eQTL.MethodTo detect single nucleotide polymorphisms (SNPs) that influence expression levels of genes associated with bipolar disorder, we jointly analysed data from a bipolar disorder GWAS (7481 cases and 9250 controls) and a genome-wide brain (cortical) eQTL (193 healthy controls) using a Bayesian statistical method, with independent follow-up replications. The identified risk SNP was then further tested for association with hippocampal volume (n = 5775) and cognitive performance (n = 342) among healthy individuals.ResultsIntegrative analysis revealed a significant association between a brain eQTL rs6088662 on chromosome 20q11.22 and bipolar disorder (log Bayes factor = 5.48; bipolar disorder P = 5.85×10(-5)). Follow-up studies across multiple independent samples confirmed the association of the risk SNP (rs6088662) with gene expression and bipolar disorder susceptibility (P = 3.54×10(-8)). Further exploratory analysis revealed that rs6088662 is also associated with hippocampal volume and cognitive performance in healthy individuals.ConclusionsOur findings suggest that 20q11.22 is likely a risk region for bipolar disorder; they also highlight the informative value of integrating functional annotation of genetic variants for gene expression in advancing our understanding of the biological basis underlying complex disorders, such as bipolar disorder.

Evaluación de intervención de alertas para riesgo cardiovascular por diclofenaco en afiliados al régimen contributivo 2014 -2015

El objetivo fue evaluar la intervención de las alertas en la prescripción de diclofenaco. Estudio observacional, comparativo, post intervención, de un antes después, en pacientes con prescripción de diclofenaco. Se evaluó la intervención de las alertas restrictivas antes y después de su implementación en los pacientes prescritos con diclofenaco y que tenían asociado un diagnóstico de riesgo cardiovascular según CIE 10 o eran mayores de 65 años. Un total de 315.135 transacciones con prescripción de diclofenaco, en 49.355 pacientes promedio mes. El 94,8% (298.674) de las transacciones fueron prescritas por médicos generales.

The paper mill chemist,

Mode of access: Internet.

Cowper memorials / Records of the Rev. John Cowper, M.A., and of other members of the family of Wm. Cowper, the poet.

Mode of access: Internet.

Virus diseases of sugar beets in California /

Mode of access: Internet.

Hamlet from the actors' standpoint. Its representatives, and a comparison of their performances.

"Authorities consulted": p. 169-170.

History of the city of Buffalo and Erie county.

Mode of access: Internet.

Stories by American authors.

v. 6. The village convict, by C. H. White. The Denver express, by A. A. Hayes. The misfortunes of Bro' Thomas Wheatley, by Lina R. Fairfax. The heartbreak cameo, by L. W. Champney. Miss Eunice's glove, by A. Webster. Brother Sebastian's friendship, by H. Frederic.--v. 7. The bishop's vagabond, by Octave Thanet. Lost, by E. Bellamy. Kirby's coals of fire, by Louise Stockton. Passages from the journal of a social wreck, by Margaret Floyd. Stella Grayland, by J. T. McKay. The image of San Donato, by Virginia W. Johnson.--v. 8. The brigade commander, by J. W. De Forest. Split zephyr, by H. A. Beers. Zerviah Hope, by Elizabeth S. Phelps. The life-magnet, by A. A. Adee. Osgood's predicament, by Elizabeth D. B. Stoddard.--v. 9. Marse Chan, by T. N. Page. Mr. Bixby's Christmas visitor, by C. S. Gage. Eli, by C. H. White. Young Strong of "The Clarion," by Milicent W. Shinn. How old Wiggins wore ship, by Captain R. T. Coffin. "--mas has come," by L. Kip.--v. 10. Pancha, by T. A. Janvier. The ablest man in the world, by E. P. Mitchell. Young Moll's peevy, by C. A. Stephens. Manmat'ha, by C. De Kay. A daring fiction, by H. H. Boyesen. The story of two lives, by Julia Schayer.

Oil audit and reuse manual for the industrial plant /

Mode of access: Internet.

The comedies, histories, and tragedies of Mr. William Shakespeare as presented at the Globe and Blackfriars theatres Photocopy.

Mode of access: Internet.

History of the city of Buffalo and Erie County : with illustrations and biographical sketches of some of its prominent men and pioneers /

Includes indexes.