Description of the larva of Mnesarete pudica (Hagen in Selys, 1853) (Odonata: Calopterygidae) and notes on known genera of South American Calopterygidae larvae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morfologia do tubo digestivo nos cupins Heterotermes tenuis (Hagen) e Coptotermes havilandi Holmgren (Isoptera, Rhinotermitidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comportamento intra-específico do cupim Heterotermes tenuis (Hagen) (Isoptera, Rhinotermitidae) em condições de laboratório

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

População e território de forrageamento de uma colônia de Heterotermes tenuis (Hagen) (Isoptera, Rhinotermitidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Secretion of salivary glands of the Brazilian termite Serritermes serrifer Hagen&Bates (Isoptera: Serritermitidae)

The salivary glands of termites are composed of several secretory acini connected by ducts. These glands, in the Brazilian termite Serritermes serrifer, were examined through the electron microscope. The ultrastructure of worker salivary acinus revealed central ductule cells and four different types of cells. Cells of type I contain an abundance of electron-lucid vacuoles of various sizes which fuse to form enormous vacuolar structures that fill up most of the cell. Cells of type II are narrow cells in which the secretion is contained in small clear vacuoles of approximately equal diameter. Both of these cellular types have numerous Golgi bodies and rough endoplasmic reticulum. Type III or parietal cells have an apical plasma membrane deeply infolded and lined by microvilli. This type of cell is located in the acinar periphery and occurs in pairs. Cells of type IV are completely filled with electrondense secretion. The secretory granules can be small in some cells or large and similar to fingerprints in others. This is the first report of the occurrence of these spiral or concentric rings of dense material in the salivary gland of Isoptera.

THE LEG EXOCRINE SYSTEM IN SERRITERMES-SERRIFER (HAGEN, 1858), PHYLOGENETIC IMPLICATIONS (ISOPTERA, SERRITERMITIDAE

The leg exocrine gland was examined in two species of Neotropical termites. Scanning microscopy studies showed a set of pores on the ventral surface of the first and second tarsomeres in all legs of Serritermes serrifer. In Heterotermes tenuis these pores are present on a sunken plate in all castes. To date, this gland has been observed only in Rhinotermitid species. The presence of leg exocrine gland provides additional evidence supporting a close phylogenetic relationship between the Serritermitidae and Rhinotermitidae.

Consumo e ganho de peso de larvas de Chrysoperla Externa (Hagen, 1861) (Neuroptera: Chrysopidae) de diferentes Populaçoes e Geraçoes de Laboratório

Several studies have been made about biology, predatory capacity, and food consumption to crisopids species, trying to get informations to use in Integrated Pest Management. Raising insects in laboratory for many generations could cause blood relation problems, even annihilate the insect mass production. In this way, the objective of this work was to determine the consumption and the weight gain to Chrysoperla externa larvae from two populations and different laboratory generations, founded by 1, 5, 10, 15 and 20 couples. By the result it was possible to conclude that larvae food consumption was higher in populations founded by greater number of couples, and it is not verified variation among populations and generations.

Secretion of salivary glands of the Brazilian termite Serritermes serrifer Hagen & Bates (Isoptera : Serritermitidae)

The salivary glands of termites are composed of several secretory acini connected by ducts. These glands, in the Brazilian termite Serritermes serrifer, were examined through the electron microscope. The ultrastructure of worker salivary acinus revealed central ductule cells and four different types of cells. Cells of type I contain an abundance of electron-lucid vacuoles of various sizes which fuse to form enormous vacuolar structures that fill up most of the cell. Cells of type II are narrow cells in which the secretion is contained in small clear vacuoles of approximately equal diameter. Both of these cellular types have numerous Golgi bodies and rough endoplasmic reticulum. Type III or parietal cells have an apical plasma membrane deeply infolded and lined by microvilli. This type of cell is located in the acinar periphery and occurs in pairs. Cells of type IV are completely filled with electrondense secretion. The secretory granules can be small in some cells or large and similar to fingerprints in others. This is the first report of the occurrence of these spiral or concentric rings of dense material in the salivary gland of Isoptera.

Loss of Genetic Variability Induced by Agroecosystems: Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) as a Case Study

Four species of green lacewings occur in Brazil, of which Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) exhibits the widest geographical distribution. Chrysoperla externa is a predatory insect that is potentially useful as a biological control agent of agricultural pests. Studies on the genetic diversity of lacewing populations are essential to reduce the environmental and economic harm that may be caused by organisms with a low ability to adapt to the adverse and/or different environmental conditions to which they are exposed. We used the cytochrome oxidase I mitochondrial gene as a molecular marker to investigate the genetic diversity of green lacewing species collected from native and agroecosystem environments. Populations derived from native areas showed higher rates of genetic variability compared to populations from agroecosystems. Demographic changes in the form of population expansion were observed in agroecosystems, whereas populations in the native environment appeared stable over time. A statistical analysis showed significant genetic structure between each of the sampled groups, combined with its complete absence within each group, corroborating each group's identity. We infer that the loss of variability exhibited by populations from the agroecosystems is the result of genetic drift by means of the founder effect, a similar effect that has been observed in other introduced populations. Agroecosystems might therefore function as exotic areas for green lacewings, even when these areas are within the normal range of the species. © 2012 Sociedade Entomológica do Brasil.

Estudos de variabilidade genética em populações de Chrysoperla externa Hagen, 1861 (Neuroptera: Chrysopidae) do estado de São Paulo, Brasil

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Eficiência de predação de Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae) sobre o tripes do amendoim Enneothrips flavens (Moulton, 1941) (Thysanoptera: Thripidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise dos genes mitocondriais COI e 16S de populações de Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae) de diferentes localidades geográficas

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Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene

Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene. Green lacewings are generalist predators, and the species Chrysoperla externa presents a great potential for use in biological control of agricultural pests due to its high predation and reproduction capacities, as well as its easy mass rearing in the laboratory. The adaptive success of a species is related to genetic variability, so that population genetic studies are extremely important in order to maximize success of the biological control. Thus, the present study used nuclear (Inter Simple Sequence Repeat - ISSR) and mitochondrial (Cytochrome Oxidase I - COI) molecular markers to estimate the genetic variability of 12 populations in the São Paulo State, Brazil, as well as the genetic relationships between populations. High levels of genetic diversity were observed for both markers, and the highest values of genetic diversity appear associated with municipalities that have the greatest areas of native vegetation. There was high haplotype sharing, and there was no correlation between the markers and the geographic distribution of the populations. The AMOVA indicated absence of genetic structure for the COI gene, suggesting that the sampled areas formed a single population unit. However, the great genetic differentiation among populations showed by ISSR demonstrates that these have been under differentiation after their expansion or may also reflect distinct dispersal behavior between males and females.

Description of the larva of Mnesarete pudica (Hagen in Selys, 1853) (Odonata: Calopterygidae) and notes on known genera of South American Calopterygidae larvae

The final instar larva of Mnesarete pudica is described and illustrated based on reared specimens collected in Brazil. This species can be distinguished from others by presenting: a) five palpal and three premental setae; b) no posterodorsal hooks on abdominal segments; c) lateral spines only in S9-10. M. pudica is compared to other South American calopterygids and biological notes are presented.