In Between : The Gap of quality L'influenza dell'insegnamento di Gurdjieff nel teatro di Peter Brook, Decalan Donnellan e Robert Lepage

Il Lavoro é incentrato sull' influenza dell'insegnamento di G. I Gurdjieff sul teatro del novecento in particolare sul lavoro di Peter Brook, Declan Donnellan e Robert Lepage

Bericht über die unter Leitung des V. z. Bef. d. G. i. d. Kgl. Pr. Staaten auf den Riesenfeldern der Stadt Berlin zu Blankenburg ausgeführten Versuchen im Jahre 1885

zusammengestellt von Jörns und Joseph Klar

Leopold der Erste : Leopold, D. G. I. Roman. Imperat.

Signatur des Originals: S 36/G03281

Austrian glacier inventory 1969 (G I)

The Austrian glacier inventory 1969 was compiled from airborne photogrammetry dating from September and October 1969. It includes not only area and surface elevation, but also a number of other parameters as aspect, maximum and minimum elevation, position, ablation and accumulation area. The dataset published here are the orginal results. In course of the compilation of the second Austrian glacier inventory, the photogrammetric data partly has been reanalysed. While in this data set snow areas connected to the glaciers and snow patches in the upper parts of the glacier have not been added to the glacier area, the second glacier inventory included the snow patches.

Chemotaxis in a lymphocyte cell line transfected with C-C chemokine receptor 2B: Evidence that directed migration is mediated by βγ dimers released by activation of Gαi-coupled receptors

Chemotaxis is mediated by activation of seven-transmembrane domain, G protein-coupled receptors, but the signal transduction pathways leading to chemotaxis are poorly understood. To identify G proteins that signal the directed migration of cells, we stably transfected a lymphocyte cell line (300-19) with G protein-coupled receptors that couple exclusively to Gαq (the m3 muscarinic receptor), Gαi (the κ-opioid receptor), and Gαs (the β-adrenergic receptor), as well as the human thrombin receptor (PAR-1) and the C-C chemokine receptor 2B. Cells expressing receptors that coupled to Gαi, but not to Gαq or Gαs, migrated in response to a concentration gradient of the appropriate agonist. Overexpression of Gα transducin, which binds to and inactivates free Gβγ dimers, completely blocked chemotaxis although having little or no effect on intracellular calcium mobilization or other measures of cell signaling. The identification of Gβγ dimers as a crucial intermediate in the chemotaxis signaling pathway provides further evidence that chemotaxis of mammalian cells has important similarities to polarized responses in yeast. We conclude that chemotaxis is dependent on activation of Gαi and the release of Gβγ dimers, and that Gαi-coupled receptors not traditionally associated with chemotaxis can mediate directed migration when they are expressed in hematopoietic cells.

RGS-GAIP, a GTPase-activating Protein for Gαi Heterotrimeric G Proteins, Is Located on Clathrin-coated Vesicles

RGS-GAIP (Gα-interacting protein) is a member of the RGS (regulator of G protein signaling) family of proteins that functions to down-regulate Gαi/Gαq-linked signaling. GAIP is a GAP or guanosine triphosphatase-activating protein that was initially discovered by virtue of its ability to bind to the heterotrimeric G protein Gαi3, which is found on both the plasma membrane (PM) and Golgi membranes. Previously, we demonstrated that, in contrast to most other GAPs, GAIP is membrane anchored and palmitoylated. In this work we used cell fractionation and immunocytochemistry to determine with what particular membranes GAIP is associated. In pituitary cells we found that GAIP fractionated with intracellular membranes, not the PM; by immunogold labeling GAIP was found on clathrin-coated buds or vesicles (CCVs) in the Golgi region. In rat liver GAIP was concentrated in vesicular carrier fractions; it was not found in either Golgi- or PM-enriched fractions. By immunogold labeling it was detected on clathrin-coated pits or CCVs located near the sinusoidal PM. These results suggest that GAIP may be associated with both TGN-derived and PM-derived CCVs. GAIP represents the first GAP found on CCVs or any other intracellular membranes. The presence of GAIP on CCVs suggests a model whereby a GAP is separated in space from its target G protein with the two coming into contact at the time of vesicle fusion.

GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of Gαi subunits

GAIP (G Alpha Interacting Protein) is a member of the recently described RGS (Regulators of G-protein Signaling) family that was isolated by interaction cloning with the heterotrimeric G-protein Gαi3 and was recently shown to be a GTPase-activating protein (GAP). In AtT-20 cells stably expressing GAIP, we found that GAIP is membrane-anchored and faces the cytoplasm, because it was not released by sodium carbonate treatment but was digested by proteinase K. When Cos cells were transiently transfected with GAIP and metabolically labeled with [35S]methionine, two pools of GAIP—a soluble and a membrane-anchored pool—were found. Since the N terminus of GAIP contains a cysteine string motif and cysteine string proteins are heavily palmitoylated, we investigated the possibility that membrane-anchored GAIP might be palmitoylated. We found that after labeling with [3H]palmitic acid, the membrane-anchored pool but not the soluble pool was palmitoylated. In the yeast two-hybrid system, GAIP was found to interact specifically with members of the Gαi subfamily, Gαi1, Gαi2, Gαi3, Gαz, and Gαo, but not with members of other Gα subfamilies, Gαs, Gαq, and Gα12/13. The C terminus of Gαi3 is important for binding because a 10-aa C-terminal truncation and a point mutant of Gαi3 showed significantly diminished interaction. GAIP interacted preferentially with the activated (GTP) form of Gαi3, which is in keeping with its GAP activity. We conclude that GAIP is a membrane-anchored GAP with a cysteine string motif. This motif, present in cysteine string proteins found on synaptic vesicles, pancreatic zymogen granules, and chromaffin granules, suggests GAIP’s possible involvement in membrane trafficking.

Uchastkovoe zemlepolʹzovanie v Rossii. Khutora i otruba, ikh rasprostranenie za desiï¸ a︡tiletie 1907-1916 g.g. i sudʹby vo vremiï¸ a︡ revoliï¸ u︡tï¸ ï¸¡sii (1917-1920 g.g.)

Mode of access: Internet.

Obshchaiï¸ a︡ polʹza avtorskago prava : s prilozhenÄ«em pisʹma k russkoÄ­ pechati Ä–miliï¸ a︡ Zoliï¸ a︡ i teksta zakonov o literaturnoÄ­ i khudozhestvennoÄ­ sobstvennosti, amerikanskago, belʹgÄ«Ä­skago i ispanskago, BernskoÄ­ konventï¸ s︡īi 1886 g. i frantï¸ s︡uzskago dekreta 1852 g. /

Mode of access: Internet.

Dictionnaire des artistes, dont nous avons des estampes, avec une notice detaillée de leurs ouvrages gravés. Leipsig, J. G. I. Breitkopf, 1778-90.

Vol 4. ends with article "Antonio Diziani"; no more published.

Obzor Bakinskoĭ neftiï¸ a︡noĭ promyshlennosti za 1897 g. i kratkiÌ„iï¸ a︡ sviï¸ e︡diï¸ e︡niÌ„iï¸ a︡ o neftiï¸ a︡noĭ promyshlennosti v Groznom i Amerikiï¸ e︡ /

Mode of access: Internet.

Khronika umstvennago i nravstvennago razvitÄ«iï¸ a︡ kishinevskikh evreev, 1773 g. po 1890 g., i obzor evreÄ­skikh blagotvoritelʹnykh uchrezï¸ h︡denÄ«Ä­ BessarbskoÄ­ gubernÄ«i

Mode of access: Internet.

Svod zakonov RossiÌ„ĭskoĭ : dopolnennyĭ po ProdolzheniÌ„iï¸ a︡m 1906, 1908, 1909 i 1910 g.g. i pozdniï¸ e︡ĭshim 1911 i 1912 g.g. /

Negative master # 5917.

Sotï¸ a︡īalizm v Bel'gÄ«i : S prilozhenÄ«em programmy i ustava partÄ«i i biblÄ«ograficheskago ukazateliï¸ a︡. Perevod s frantï¸ s︡uzskago /

At head of title: Vandervel'd i Destre.