917 resultados para ESTACAO ECOLOGICA ESTADUAL DE WENCESLAU GUIMARAES


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We are presenting here p/n junctions obtained with a modified opened liquid-phase epitaxy (LPE) system, used to diffuse indium antimonide (InSb) doped with Cd over InSb doped with Te wafers, in order to make InSb infrared (IR) sensors. This technique has several advantages: the diffusion can be performed in bigger substrate areas improving the device production; this method decreases the device manipulation, decreasing human mistakes and increasing the process reproducibility. The opened LPE in this work produced sensors in the first case with vapor of the diffusion material, coming from a microholed carbon boat full of the diffusion material, over which is positioned the substrate at atmospheric pressure. In the second, the diffusion material is on the bottom of a quartz recipient, and the InSb/Te wafer works as its cover, and vacuum was used. The IR sensors produced with the first method measured 8.9 x 10(7) cm Hz(1/2)/W as detectivity value and higher IR spectral response at 4.6 mu m, and those produced with the second 2.8 x 10(9) cm Hz(1/2)/W, at 4.4 mu m. Besides the electrical-optical properties, the structural properties of diffused layers were investigated by X-ray diffraction (XRD), scanning electron and atomic force microscopy (SEM, AFM), energy-dispersive and secondary ion mass spectroscopy (EDS, SIMS). (C) 2007 Elsevier B.V. All rights reserved.

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Apparent amino acid availability coefficients and protein digestibility of four animal products [fish meal (FM), meat and bone meal (MBM), poultry by-product and feather meal] and four plant protein-rich products [soybean meal (SBM), cottonseed meal-28, cottonseed meal-38 and corn gluten meal (CGM)] were determined for Nile tilapia, Oreochromis niloticus. Ingredients were incorporated to a practical reference diet at a 7 : 3 ratio (70% of reference diet and 30% of test ingredient). Chromic oxide was used as external digestibility marker. Among animal products poultry by-product meal (PBM; 89.7%) and FM (88.6%) presented the highest apparent protein digestibility (APD) while MBM (78.4%) and feather meal (78.5%) presented the lowest APD. Among plant protein-rich products CGM (91.4%) and SBM (92.4%) presented the highest APD values while cottonseed meal-28 presented the lowest APD (78.6%). Average apparent amino acid availability of feed ingredients was similar to protein digestibility with 92.3%, 89.6%, 73.4%, 80.7%, 88.9%, 84.4%, 91.2% and 79.7% values for SBM, CGM, cottonseed meal-28 and 38, FM, MBM, PBM and feather meal respectively. These results indicate that O. niloticus is able to utilize efficiently different feedstuffs.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Objective: Periodontitis is a well-appreciated example of leukocyte-mediated bone loss and inflammation with pathogenic features similar to those observed in other inflammatory diseases, such as arthritis. Since Tacrolimus, is an immunomodulatory drug used for the treatment of some cases of arthritis, we hypothesized that it may modulate periodontal disease.Design: Using a murine model of ligature-induced periodontal disease, we assessed the effects of daily administrations of Tacrolimus (1 mg/kg body weight) on bone loss, enzymatic (myeloperoxidase) analysis, differential white blood cells counts, airpouch exudate and cytokine expression for 5-30 days.Results: Radiographic, enzymatic (myeloperoxidase) and histological analysis revealed that Tacrolimus reduced the severity of periodontitis. More specifically, Tacrolimus suppressed the expression of serum interleukin (IL-1 beta), tumour necrosis factor (TNF-alpha), IL-6, airpouch exudate PGE(2) and leukocytosis usually observed after the induction of periodontitis. Tacrolimus treatment in periodontitis-induced rats conferred protection against the inflammation-induced tissue and bone loss associated with periodontitis, through a mechanism involving IL-1 beta, TNF-alpha and IL-6.Conclusions: the effects of Tacrolimus on periodontal disease pathogenesis may provide clues to a novel approach to host modulation therapy in destructive periodontal disease. (C) 2007 Elsevier Ltd. All rights reserved.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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A pitaya é uma cactácea originária das florestas tropicais americanas que produz frutos exóticos bem apreciados e comercializados, principalmente no continente asiático. Contudo, ainda é uma espécie vegetal que demanda informações técnicas de cultivo nas áreas agrícolas brasileiras, motivando pesquisas em várias áreas do conhecimento. Objetivou-se avaliar diferentes substratos no enraizamento de cladódios imersos e não imersos no regulador de crescimento, ácido naftalenacético (ANA), na formação e desenvolvimento inicial de raízes em estacas de Hylocereus undatus, resultando em mudas mais vigorosas e de melhor qualidade. O experimento foi conduzido sob ripado no Departamento de Produção Vegetal - setor de Horticultura da Faculdade de Ciências Agronômicas no campus de Botucatu - UNESP. O delineamento experimental foi o inteiramente aleatorizado em esquema fatorial 4 x 2 (substratos x condições) com quatro repetições. Após 60 dias da instalação do experimento foram avaliados: massa fresca e seca da parte aérea, massa fresca e seca das raízes e o comprimento da maior raiz. O crescimento e desenvolvimento radicular inicial de mudas não são influenciados pelo regulador vegetal ANA. Os substratos com a presença de areia são os mais adequados para a formação de mudas vigorosas e de boa qualidade. A mistura areia + esterco bovino proporciona maior acúmulo de fitomassa no sistema radicular de pitaya (H. undatus).

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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The ultrastructural characteristics of the organelles present in Serrasalmus spilopleura oogonia and oocytes undergoing primary growth were described in detail, considering its role in the nuclear and cytoplasmic metabolic processes that occur in these cell types. Even though these cells do not significantly differ from those similar to them that are found in other teleost groups, the analysis of their ultrastructure makes available new data on the reproductive biology of Characiformes. (C) 2001 Harcourt Publishers Ltd.

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The morphophysiological changes that occur during oocyte primary growth in Serrasalmus spilopleura were studied using ultrastructural cytochemical techniques. In the previtellogenic oocytes endoplasmic reticulum components, Golgi complex cisternae and vesicles, lysosomes, multivesicular bodies and some electron-dense vesicles react to acid phosphatase (AcPase) detection. The endoplasmic reticulum components, Golgi complex cisternae and vesicles also react to osmium tetroxide and potassium iodide impregnation (KI). These structures, except for the Golgi complex cisternae, are strongly contrasted by osmium tetroxide and zinc iodide impregnation (ZIO). Some electron-dense vesicles are ZIO-stained, while microvesicles in the multivesicular bodies and other large isolated cytoplasmic vesicles are contrasted by KI. At primary oocyte growth, the activity of the endomembranous system and the proliferation of membranous organelles are intense. The biosynthetic pathway of the lysosomal proteins such as acid phosphatase, involves the endoplasmic reticulum, Golgi complex, vesicles with inactive hydrolytic enzymes and, finally, the lysosomes. The oocyte endomembranous system have reduction capacity and are involved in the metabolism of rich in SH groups. (c) 2005 Published by Elsevier Ltd.

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The post-larval development of the mud crab Eurytium limosum was studied under laboratory conditions by using the offspring of ovigerous females collected at the Comprido River mangrove, SP, Brazil. The first crab stage is fully described and the juvenile development, until crab stage 10, is examined with emphasis on morphological change, sexual differentiation and growth patterns. The carapace of the first crab stage is nearly square as observed in other xanthids, becoming similar to adults only at stage 15. The sexes can be distinguished from stage four, based on the number of pleopods and their morphology. While the intermoult period increases, the moult percentage decreases at each stage. The abdominal allometric growth is sex-dependent, with males showing a negative (b=0.71) and females an isometric (b=0.95) relative growth pattern. Male gonopods undergo a positive allometric growth, and their shape changes remarkably until sexual maturity. The cheliped dentition can be observed after stage 4. Regardless of sex, most crabs have a molariform right cheliped, which is thought to aid the handling of asymmetric prey such as gastropods.

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The size at onset of maturity of Eurytium limosum from a subtropical mangrove in Brazil was investigated. In this species, sexual maturity for males can be indicated by the allometric growth and gonopod length. For females, the morphological sexual maturity can only be externally verified through the relative quantity of setae along the abdominal margins and pleopods. Internally, gonad development was also examined. The size at which half of the population is physiologically mature was 11.6 mm of CW for females and 12.3 mm of CW for males. The values for the morphological and physiological maturity are very similar, indicating that the development of the secondary sexual characters is synchronized with the achievement of the physiological maturity for E. limosum.

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The occurrence of brachiopods in Cenozoic rocks of the Pelotas Basin is known since 1862. In spite of that, detailed systematic and taphonomic studies are still missing. Investigations made a half century ago, have suggested that these brachiopods could belong to Bouchardia cf. zitteli, a species found in the San Julian Formation, Late Oligocene, Argentina. Our data suggest that those brachiopods may resemble Bouchardia transplatina. In the Uruguayan portion of the Pelotas Basin B. transplatina is known in rocks of the Camacho Formation, Miocene. In addition, small recrystallized shells of brachiopods were also recovered from three Petrobras boreholes (2PJ-1-RS, 2PN-1-RS, and 2GA-1-RS) from the Pelotas Basin. Brachiopods come from the interval of 130 to 150 meters within the Miocene Henryhowella evax Zone. Despite the degree of taphonomic modiication of those brachiopod shells they indubitably belong to Bouchardia sp. This is noteworthy for various reasons: 1- Bouchardia is a brachiopod with warm water afinities. Presently, extant members of this genus are unknown in latitudes up to 34[degree]S, with the main records at 23[degree]S. 2- Although occurring in depths down to 200 meters, the living member (Bouchardia rosea) of this genus is most abundant in shallow platformal, nutrient-rich waters. 3- The occurrence of Bouchardia in the Miocene of the Pelotas Basin indicates that, at least to the interval of Henryhowella evax Zone, warm waters of the Brazilian currents prevail. This interpretation is in strong accordance with other paleoeoceanographic and paleoclimatic data offered by various groups of co-occurring microfossils, such as ostracodes and foraminifers.

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Quinolizidine alkaloids (QAs) are secondary compounds found in seeds of many species of plants, possibly protecting them against pathogens and seed predators. QAs were isolated from Ormosia arborea seeds and bioassayed against red-rumped agoutis (Dasyprocta leporina, Rodentia: Caviomorpha) to verify if they inhibit seed predation and food hoarding (seed dispersal). Three treatments were used: (1) seeds of O. arborea, (2) palatable seeds of Mimusops coriacea (Sapotaceae) treated with MeOH, and (3) seeds of M. coriacea treated with QAs dissolved in MeOH in similar concentration to that present in O. arborea. Palatable seeds were significantly more preyed upon than seeds treated with QAs and Ormosia seeds, but QAs did not influence hoarding behavior. QAs in O. arborea may have a strong effect in avoiding seed predation by rodents, without reducing dispersal.