926 resultados para EEG, fMRI, sinestesia
Resumo:
Background: Seizures and interictal spikes in mesial temporal lobe epilepsy (MTLE) affect a network of brain regions rather than a single epileptic focus. Simultaneous electroencephalography and functional magnetic resonance imaging (EEG-fMRI) studies have demonstrated a functional network in which hemodynamic changes are time-locked to spikes. However, whether this reflects the propagation of neuronal activity from a focus, or conversely the activation of a network linked to spike generation remains unknown. The functional connectivity (FC) changes prior to spikes may provide information about the connectivity changes that lead to the generation of spikes. We used EEG-fMRI to investigate FC changes immediately prior to the appearance of interictal spikes on EEG in patients with MTLE. Methods/principal findings: Fifteen patients with MTLE underwent continuous EEG-fMRI during rest. Spikes were identified on EEG and three 10 s epochs were defined relative to spike onset: spike (0–10 s), pre-spike (−10 to 0 s), and rest (−20 to −10 s, with no previous spikes in the preceding 45s). Significant spike-related activation in the hippocampus ipsilateral to the seizure focus was found compared to the pre-spike and rest epochs. The peak voxel within the hippocampus ipsilateral to the seizure focus was used as a seed region for FC analysis in the three conditions. A significant change in FC patterns was observed before the appearance of electrographic spikes. Specifically, there was significant loss of coherence between both hippocampi during the pre-spike period compared to spike and rest states. Conclusion/significance: In keeping with previous findings of abnormal inter-hemispheric hippocampal connectivity in MTLE, our findings specifically link reduced connectivity to the period immediately before spikes. This brief decoupling is consistent with a deficit in mutual (inter-hemispheric) hippocampal inhibition that may predispose to spike generation.
Resumo:
La consolidation est le processus qui transforme une nouvelle trace mnésique labile en une autre plus stable et plus solide. Une des tâches utilisées en laboratoire pour l’exploration de la consolidation motrice dans ses dimensions comportementale et cérébrale est la tâche d’apprentissage de séquences motrices. Celle-ci consiste à reproduire une même série de mouvements des doigts, apprise de manière implicite ou explicite, tout en mesurant l’amélioration dans l’exécution. Les études récentes ont montré que, dans le cas de l’apprentissage explicite de cette tâche, la consolidation de la trace mnésique associée à cette nouvelle habileté dépendrait du sommeil, et plus particulièrement des fuseaux en sommeil lent. Et bien que deux types de fuseaux aient été décrits (lents et rapides), le rôle de chacun d’eux dans la consolidation d’une séquence motrice est encore mal exploré. En effet, seule une étude s’est intéressée à ce rôle, montrant alors une implication des fuseaux rapides dans ce processus mnésique suite à une nuit artificiellement altérée. D’autre part, les études utilisant l’imagerie fonctionnelle (IRMf et PET scan) menées par différentes équipes dont la notre, ont montré des changements au niveau de l’activité du système cortico-striatal suite à la consolidation motrice. Cependant, aucune corrélation n’a été faite à ce jour entre ces changements et les caractéristiques des fuseaux du sommeil survenant au cours de la nuit suivant un apprentissage moteur. Les objectifs de cette thèse étaient donc: 1) de déterminer, à travers des enregistrements polysomnographiques et des analyses corrélationnelles, les caractéristiques des deux types de fuseaux (i.e. lents et rapides) associées à la consolidation d’une séquence motrice suite à une nuit de sommeil non altérée, et 2) d’explorer, à travers des analyses corrélationnelles entre les données polysomnographiques et le signal BOLD (« Blood Oxygenated Level Dependent »), acquis à l’aide de l’imagerie par résonance magnétique fonctionnelle (IRMf), l’association entre les fuseaux du sommeil et les activations cérébrales suite à la consolidation de la séquence motrice. Les résultats de notre première étude ont montré une implication des fuseaux rapides, et non des fuseaux lents, dans la consolidation d’une séquence motrice apprise de manière explicite après une nuit de sommeil non altérée, corroborant ainsi les résultats des études antérieures utilisant des nuits de sommeil altérées. En effet, les analyses statistiques ont mis en évidence une augmentation significative de la densité des fuseaux rapides durant la nuit suivant l’apprentissage moteur par comparaison à la nuit contrôle. De plus, cette augmentation corrélait avec les gains spontanés de performance suivant la nuit. Par ailleurs, les résultats de notre seconde étude ont mis en évidence des corrélations significatives entre l’amplitude des fuseaux de la nuit expérimentale d’une part et les gains spontanés de performance ainsi que les changements du signal BOLD au niveau du système cortico-striatal d’autre part. Nos résultats suggèrent donc un lien fonctionnel entre les fuseaux du sommeil, les gains de performance ainsi que les changements neuronaux au niveau du système cortico-striatal liés à la consolidation d’une séquence motrice explicite. Par ailleurs, ils supportent l’implication des fuseaux rapides dans ce type de consolidation ; ceux-ci aideraient à l’activation des circuits neuronaux impliqués dans ce processus mnésique et amélioreraient par la même occasion la consolidation motrice liée au sommeil.
Resumo:
The use of multimodal neuroimaging techniques has been helpful in the investigation of epileptogenic zone in patients with refractory epilepsies. This work aims to describe an ictal event during EEG-fMRI performed simultaneously in a 39-year-old man with refractory epilepsy. The EEG data were recorded at a sampling rate of 5 kHz, using a BrainAmp (BrainProducts, München, Germany) amplifier, with 64 MR (magnetic resonance) compatible Ag/AgCl electrodes. MR images were acquired using a 3T scanner in 3 sequences of 6 minutes of echo-planar images (EPIs), with TR = 2s, being the last sequence stopped after the ictal event. The EEG was corrected for gradient and pulse artifacts using the Brain Vision Analyzer2 software (BrainProducts), and the functional images were realigned, slice-timing corrected, normalized and smoothed. The start of the ictal changes was used for the evaluation of the BOLD response in MR images, using a t-test with a minimum cluster of 5 voxels, p <0.005 (T>2.5). The patient had a partial complex seizure, as noted by neurologist. The fMRI data showed positive BOLD responses (activation) in dysplastic areas, but showed the most significant activation outside the lesion, in areas compatible with secondary spread of the epileptic focus, probably caused by motor reaction also observed during the seizure. As a conclusion, we note that the technique of EEG-fMRI can detect the epileptogenic zone in patients with refractory epilepsy, but areas of dissemination of primary epileptogenic focus may show significant activation, introducing additional difficulties to the interpretation of the results
Resumo:
Focal onset epilepsies most often occur in the temporal lobes. To improve diagnosis and therapy of patients suffering from pharmacoresistant temporal lobe epilepsy it is highly important to better understand the underlying functional and structural networks. In mesial temporal lobe epilepsy (MTLE) widespread functional networks are involved in seizure generation and propagation. In this study we have analyzed the spatial distribution of hemodynamic correlates (HC) to interictal epileptiform discharges on simultaneous EEG/fMRI recordings and relative grey matter volume (rGMV) reductions in 10 patients with MTLE. HC occurred beyond the seizure onset zone in the hippocampus, in the ipsilateral insular/operculum, temporo-polar and lateral neocortex, cerebellum, along the central sulcus and bilaterally in the cingulate gyrus. rGMV reductions were detected in the middle temporal gyrus, inferior temporal gyrus and uncus to the hippocampus, the insula, the posterior cingulate and the anterior lobe of the cerebellum. Overlaps between HC and decreased rGMV were detected along the mesolimbic network ipsilateral to the seizure onset zone. We conclude that interictal epileptic activity in MTLE induces widespread metabolic changes in functional networks involved in MTLE seizure activity. These functional networks are spatially overlapping with areas that show a reduction in relative grey matter volumes.
Resumo:
Combined EEG/fMRI recordings offer a promising opportunity to detect brain areas with altered BOLD signal during interictal epileptic discharges (IEDs). These areas are likely to represent the irritative zone, which is itself a reflection of the epileptogenic zone. This paper reports on the imaging findings using independent component analysis (ICA) to continuously quantify epileptiform activity in simultaneously acquired EEG and fMRI. Using ICA derived factors coding for the epileptic activity takes into account that epileptic activity is continuously fluctuating with each spike differing in amplitude, duration and maybe topography, including subthreshold epileptic activity besides clear IEDs and may thus increase the sensitivity and statistical power of combined EEG/fMRI in epilepsy. Twenty patients with different types of focal and generalized epilepsy syndromes were investigated. ICA separated epileptiform activity from normal physiological brain activity and artifacts. In 16/20 patients, BOLD correlates of epileptic activity matched the EEG sources, the clinical semiology, and, if present, the structural lesions. In clinically equivocal cases, the BOLD correlates aided to attribute proper diagnosis of the underlying epilepsy syndrome. Furthermore, in one patient with temporal lobe epilepsy, BOLD correlates of rhythmic delta activity could be employed to delineate the affected hippocampus. Compared to BOLD correlates of manually identified IEDs, the sensitivity was improved from 50% (10/20) to 80%. The ICA EEG/fMRI approach is a safe, non-invasive and easily applicable technique, which can be used to identify regions with altered hemodynamic effects related to IEDs as well as intermittent rhythmic discharges in different types of epilepsy.
Resumo:
There have been numerous attempts to reveal the neurobiological basis of schizophrenia spectrum disorders. Results however, remain as heterogeneous as the schizophrenia spectrum disorders itself. Therefore, one aim of this thesis was to divide patients affected by this disorder into subgroups in order to homogenize the results of future studies. In a first study it is suggested that psychopathological rating scales should focus on symptoms-clusters that may have a common neurophysiological background. The here presented Bern Psychopathology Scale (BPS) proposes that alterations in three wellknown brain systems (motor, language, and affective) are largely leading to the communication failures observable on a behavioral level, but also - as repeatedly hypothesized - to dysconnectivity within and between brain systems in schizophrenia spectrum disorders. The external validity of the motor domain in the BPS was tested against the objective measure of 24 hours wrist actigraphy, in a second study. The subjective, the quantitative, as well as the global rating of the degree of motor disorders in this patient group showed significant correlations to the acquired motor activity. This result confirmed in a first step the practicability of the motor domain of the BPS, but needs further validation regarding pathological brain alterations. Finally, in a third study (independent from the two other studies), two cerebral Resting State Networks frequently altered in schizophrenia were investigated for the first time using simultaneous EEG/fMRI: The well-known default mode network and the left working memory network. Besides the changes in these fMRI-based networks, there are well-documented findings that patients exhibit alterations in EEG spectra compared to healthy controls. However, only through the multimodal approach it was possible to discover that patients with schizophrenia spectrum disorders have a slower driving frequency of the Resting State Networks compared to the matched healthy controls. Such a dysfunctional coupling between neuronal frequency and functional brain organization could explain in a uni- or multifactorial way (dysfunctional cross-frequency coupling, maturational effects, vigilance fluctuations, task-related suppression), how the typical psychotic symptoms might occur. To conclude, the major contributions presented in this thesis were on one hand the development of a psychopathology rating scale that is based on the assumption of dysfunctional brain networks, as well as the new evidence of a dysfunctional triggering frequency of Resting State Networks from the simultaneous EEG/fMRI study in patients affected by a schizophrenia spectrum disorder.
Resumo:
OBJECTIVE Epilepsy is increasingly considered as the dysfunction of a pathologic neuronal network (epileptic network) rather than a single focal source. We aimed to assess the interactions between the regions that comprise the epileptic network and to investigate their dependence on the occurrence of interictal epileptiform discharges (IEDs). METHODS We analyzed resting state simultaneous electroencephalography-functional magnetic resonance imaging (EEG-fMRI) recordings in 10 patients with drug-resistant focal epilepsy with multifocal IED-related blood oxygen level-dependent (BOLD) responses and a maximum t-value in the IED field. We computed functional connectivity (FC) maps of the epileptic network using two types of seed: (1) a 10-mm diameter sphere centered in the global maximum of IED-related BOLD map, and (2) the independent component with highest correlation to the IED-related BOLD map, named epileptic component. For both approaches, we compared FC maps before and after regressing out the effect of IEDs in terms of maximum and mean t-values and percentage of map overlap. RESULTS Maximum and mean FC maps t-values were significantly lower after regressing out IEDs at the group level (p < 0.01). Overlap extent was 85% ± 12% and 87% ± 12% when the seed was the 10-mm diameter sphere and the epileptic component, respectively. SIGNIFICANCE Regions involved in a specific epileptic network show coherent BOLD fluctuations independent of scalp EEG IEDs. FC topography and strength is largely preserved by removing the IED effect. This could represent a signature of a sustained pathologic network with contribution from epileptic activity invisible to the scalp EEG.
Resumo:
We review the use of neural field models for modelling the brain at the large scales necessary for interpreting EEG, fMRI, MEG and optical imaging data. Albeit a framework that is limited to coarse-grained or mean-field activity, neural field models provide a framework for unifying data from different imaging modalities. Starting with a description of neural mass models we build to spatially extended cortical models of layered two-dimensional sheets with long range axonal connections mediating synaptic interactions. Reformulations of the fundamental non-local mathematical model in terms of more familiar local differential (brain wave) equations are described. Techniques for the analysis of such models, including how to determine the onset of spatio-temporal pattern forming instabilities, are reviewed. Extensions of the basic formalism to treat refractoriness, adaptive feedback and inhomogeneous connectivity are described along with open challenges for the development of multi-scale models that can integrate macroscopic models at large spatial scales with models at the microscopic scale.
Resumo:
Progress in functional neuroimaging of the brain increasingly relies on the integration of data from complementary imaging modalities in order to improve spatiotemporal resolution and interpretability. However, the usefulness of merely statistical combinations is limited, since neural signal sources differ between modalities and are related non-trivially. We demonstrate here that a mean field model of brain activity can simultaneously predict EEG and fMRI BOLD with proper signal generation and expression. Simulations are shown using a realistic head model based on structural MRI, which includes both dense short-range background connectivity and long-range specific connectivity between brain regions. The distribution of modeled neural masses is comparable to the spatial resolution of fMRI BOLD, and the temporal resolution of the modeled dynamics, importantly including activity conduction, matches the fastest known EEG phenomena. The creation of a cortical mean field model with anatomically sound geometry, extensive connectivity, and proper signal expression is an important first step towards the model-based integration of multimodal neuroimages.
Resumo:
This thesis addresses two major topics in neuroscience literature and drawbacks from existing literature are addressed by utilising state space models and Bayesian estimation techniques. Particle filter-based joint estimation of the physiological model for time-series analysis of fMRI data is demonstrated first in the thesis and secondly the Granger causality-based effective connectivity analysis of EEG data is investigated.
Resumo:
Gamma zero-lag phase synchronization has been measured in the animal brain during visual binding. Human scalp EEG studies used a phase locking factor (trial-to-trial phase-shift consistency) or gamma amplitude to measure binding but did not analyze common-phase signals so far. This study introduces a method to identify networks oscillating with near zero-lag phase synchronization in human subjects.
Resumo:
Several divergent cortical mechanisms generating multistability in visual perception have been suggested. Here, we investigated the neurophysiologic time pattern of multistable perceptual changes by means of a simultaneous recording with electroencephalography (EEG) and functional magnetic resonance imaging (fMRI). Volunteers responded to the subjective perception of a sudden change between stable patterns of illusionary motion (multistable transition) during a stroboscopic paradigm. We found a global deceleration of the EEG frequency prior to a transition and an occipital-accentuated acceleration after a transition, as obtained by low-resolution electromagnetic tomography analysis (LORETA) analysis. A decrease in BOLD response was found in the prefrontal cortex before, and an increase after the transitions was observed in the right anterior insula, the MT/V5 regions and the SMA. The thalamus and left superior temporal gyrus showed a pattern of decrease before and increase after transitions. No such temporal course was found in the control condition. The multimodal approach of data acquisition allows us to argue that the top-down control of illusionary visual perception depends on selective attention, and that a diminution of vigilance reduces selective attention. These are necessary conditions to allow for the occurrence of a perception discontinuity in absence of a physical change of the stimulus.