DEK oncogene expression during normal hematopoiesis and in Acute Myeloid Leukemia (AML)

DEK is important in regulating cellular processes including proliferation, differentiation and maintenance of stem cell phenotype. The translocation t(6;9) in Acute Myeloid Leukemia (AML), which fuses DEK with NUP214, confers a poor prognosis and a higher risk of relapse. The over-expression of DEK in AML has been reported, but different studies have shown diminished levels in pediatric and promyelocytic leukemias. This study has characterized DEK expression, in silico, using a large multi-center cohort of leukemic and normal control cases. Overall, DEK was under-expressed in AML compared to normal bone marrow (NBM). Studying specific subtypes of AML confirmed either no significant change or a significant reduction in DEK expression compared to NBM. Importantly, the similarity of DEK expression between AML and NBM was confirmed using immunohistochemistry analysis of tissue mircorarrays. In addition, stratification of AML patients based on median DEK expression levels indicated that DEK showed no effect on the overall survival of patients. DEK expression during normal hematopoiesis did reveal a relationship with specific cell types implicating a distinct function during myeloid differentiation. Whilst DEK may play a potential role in hematopoiesis, it remains to be established whether it is important for leukemagenesis, except when involved in the t(6;9) translocation.

Overproduction of the english definitive article by portuguese learners: a cross-sectional study

The English article system is actually so complex that it presents many challenges for most non-native learners of English. The main difficulty of Portuguese learners, despite the numerous similarities between the two article systems, is noticeable in a marked tendency to produce the definite article where native speakers of English would not use it. This article reports the results of a cross-sectional study which examined the English definite article overproduction by a group of 12 Portuguese EFL learners with at least seven years of English instruction. The prediction is that these learners will exhibit evidence of transferring L1 features to their interlanguage when they overuse the definite article. The data were collected by means of a gap-filling task and a composition. The results found, as predicted, that these learners overused the in generic contexts. It is argued that this overuse is directly tied to and can be explained by transfer to somewhere and conceptual transfer principles.

Hematopoiesis in the Drosophila larva: beyond the lymph gland

All coelomate animals possess a population of cells that do not make part of an organ and instead freely flow inside the body cavity. These cells, termed hemocytes (in invertebrates) or blood cells (in vertebrates), are involved in varied functions including immune response, clearance of apoptotic cells and distribution of nutrient and gases (Grigorian & Hartenstein 2013).(...)

Wildlife of Britain : the definitive visual guide.

Aporta información sobre la riqueza de la vida silvestre en el Reino Unido. Se centra en la actividad que puede hacerse para ayudar a la conservación de esta diversidad. Hay secciones de información sobre hábitats, precedido por un mapa de la geología de las Islas Británicas, para establecer el contexto. A continuación siguen las secciones de información sobre las costas, agua dulce, montañas, bosques y tierras agrícolas a través de las cuatro estaciones. Todas las fechas y los lugares están ilustradas por fotografías. Tiene glosario e índice alfabético.

Art : the definitive visual guide.

Guía de pintura y escultura, desde la prehistoria hasta la actualidad, con más de dos mil quinientos cuadros y esculturas más conocidos del mundo. Empieza con una breve sección que detalla cómo los artistas usan el color, la composición, la luz y la sombra, la perspectiva, y diferentes medios para llevar sus ideas a buen término. Se explica claramente cada movimiento artístico, junto con sus orígenes e influencias, estilos y técnicas. Explora temas comunes, tales como paisajes, desnudos y animales. Tiene glosario e índice alfabético.

The adult learner : the definitive classic in adult education and human resource development.

Es una lectura esencial para los profesionales del aprendizaje de adultos, estudiantes y profesionales de los recursos humanos, pues proporciona un marco teórico para la comprensión de los problemas del aprendizaje de adultos, tanto en los entornos docentes como en los lugares de trabajo. Se divide en tres partes: la primera parte estudia 'Las raíces de la andragogía', se traza el desarrollo de esta teoría y las características de los estudiantes adultos; en la segunda parte 'Los avances en el aprendizaje de adultos', se explican sus perspectivas de futuro en la investigación y la práctica y, en la última parte, 'Practica en el aprendizaje de adultos', se presentan lecturas seleccionadas que desarrollan los aspectos específicos de la andragogía en la práctica y que incluye estrategias para implementar los supuestos básicos, para adaptar el aprendizaje a las diferencias individuales, y para implementar la educación de adultos en las organizaciones. De especial interés son dos instrumentos de autoevaluación, 'the Core Competency Diagnostic and Planning Guide' y 'the Personal Adult Learning Style Inventory' que permiten al lector ponerse en camino del desarrollo personal en el aprendizaje de adultos.

Fluxing the logic of definitive differences

Focusing on Fluxus, a loosely knit association of artists from America, Europe and Asia whose work centers around intermediality, this article explores the notion of relationality without relata. Intermediality refers to works that fall conceptually between media – such as visual poetry or action music – as well as between the general area of art media and those of life media(Higgins). Departing from two Fluxus intermedia – the event score, a performative score in the form of words, and the Fluxkit, a performative score in the form of objects – I investigate the logic of co-constitutivity within which every element is both subject and object, both constitutive and constituted. To be more precise, I trace the cross-categorial interplay of differences that explodes the logico-linguistic structure of binary oppositions, such as those between foreground and background, word and action, sound and silence, identity and alterity. Aided by Jacques Derrida’s concept of ‘de-centered play’ and Shigenori Nagatomo’s concept of ‘interfusion’ this article seeks to articulate the ways in which the Fluxus works mobilise the ‘silent background’ to dismantle the dualistic logic of definite differences.

Genetic, epidemiological and biological analysis of interleukin-10 promoter single-nucleotide polymorphisms suggests a definitive role for-819C/T in leprosy susceptibility

Leprosy is a complex infectious disease influenced by genetic and environmental factors. The genetic contributing factors are considered heterogeneous and several genes have been consistently associated with susceptibility like PARK2, tumor necrosis factor (TNF), lymphotoxin-alpha (LTA) and vitamin-D receptor (VDR). Here, we combined a case-control study (374 patients and 380 controls), with meta-analysis (5 studies; 2702 individuals) and biological study to test the epidemiological and physiological relevance of the interleukin-10 (IL-10) genetic markers in leprosy. We observed that the -819T allele is associated with leprosy susceptibility either in the case-control or in the meta-analysis studies. Haplotypes combining promoter single-nucleotide polymorphisms also implicated a haplotype carrying the -819T allele in leprosy susceptibility (odds ratio (OR) = 1.40; P = 0.01). Finally, we tested IL-10 production in peripheral blood mononuclear cells stimulated with Mycobacterium leprae antigens and found that -819T carriers produced lower levels of IL-10 when compared with noncarriers. Taken together, these data suggest that low levels of IL-10 during the disease outcome can drive patients to a chronic and unprotective response that culminates with leprosy.

Extramedullary hematopoiesis in a case of benign mixed mammary tumor in a female dog: cytological and histopathological assessment

Backgroud: Extramedullary hematopoiesis (EMH) is defined as the presence of hematopoietic stem cells such as erythroid and myeloid lineage plus megakaryocytes in extramedullary sites like liver, spleen and lymph nodes and is usually associated with either bone marrow or hematological disorders. Mammary EMH is a rare condition either in human and veterinary medicine and can be associated with benign mixed mammary tumors, similarly to that described in this case.Case presentation: Hematopoietic stem cells were found in a benign mixed mammary tumor of a 7-year-old female mongrel dog that presents a nodule in the left inguinal mammary gland. The patient did not have any hematological abnormalities. Cytological evaluation demonstrated two distinct cell populations, composed of either epithelial or mesenchymal cells, sometimes associated with a fibrillar acidophilic matrix, apart from megakaryocytes, osteoclasts, metarubricytes, prorubricytes, rubricytes, rubriblasts, promyelocytes, myeloblasts. Histological examination confirmed the presence of an active hematopoietic bone marrow within the bone tissue of a benign mammary mixed tumor.Conclusions: EMH is a rare condition described in veterinary medicine that can be associated with mammary mixed tumors. It's detection can be associated with several neoplastic and non-neoplastic mammary lesions, i.e. osteosarcomas, mixed tumors and bone metaplasia.

IMMUNOHISTOCHEMICAL CHARACTERIZATION OF PROBABLE INTRAVASCULAR HEMATOPOIESIS IN THE VASA RECTAE OF THE RENAL MEDULLA IN ACUTE TUBULAR-NECROSIS

Immunohistochemistry was applied to identify the nature of the nucleated cells that accumulate in the vasa rectae of the corticomedullary junction in acute tubular necrosis. In all 6 cases studied, there were intravascular cells that reacted with monoclonal antibodies to erythroblast, macrophages, myeloid cells, T and B lymphocytes and rave megakaryocytes. The findings are consistent with the occurrence of intravascular haematopoiesis in the renal medulla in acute tubular necrosis.