A study on length-weight relationship, food and feeding habits of Indian scad, Decapterus russelli (Ruppel, 1830) along the northwest coast of India

Length and weight relationship of Decapterus russelli (Ruppell, 1830) is worked out to be W = 0.00312 L³ which indicates the isometric growth of the fish. Study on food and feeding habits revealed that the species is carnivorous, pelagic, feeding primarily on small crustaceans and small fish species, viz. Acetes indicus, ostracods, Apogon sp., Leiognathus sp., sciaenids, Netnipterus japonicas, Myctophid sp., Trichiurus sp., Therapon sp., D.russelli and occasionally on prawns. It is a selective feeder on Aeetes indicus.

Use of eye lens diameter and weight as an age indicator in the carangid fish, Decapterus russelli (Pisces: Carangidae) from Gulf of Oman: preliminary observation

Specimens of Decapterus russelli have been collected from Lema, north of the Gulf of Oman. The ocular lens diameter and weight were tested as an additional age indicator to those already in use. The results showed that this technique could be adopted for determining the age of the species Decapterus russelli when the specimens are in the second year of age in case of eye lens diameter. On the other hand, eye lens weight failed to separate between the four age groups observed. The method is especially useful for age determination when otolith or scale ring are not visible or when false rings give erroneous reading.

cDNA sequence and molecular modeling of a nerve growth factor from Bothrops jararacussu venomous gland

The complete nucleotide sequence of a nerve growth factor precursor from Bothrops jararacussu snake (Bj-NGF) was determined by DNA sequencing of a clone from cDNA library prepared from the poly(A) + RNA of the venom gland of B.jararacussu. cDNA encoding Bj-NGF precursor contained 723 bp in length, which encoded a prepro-NGF molecule with 241 amino acid residues. The mature Bj-NGF molecule was composed of I 18 amino acid residues with theoretical pI and molecular weight of 8.31 and 13,537, respectively. Its amino acid sequence showed 97%, 96%, 93%, 86%, 78%, 74%, 76%, 76% and 55% sequential similarities with NGFs from Crotalus durissus terrificus, Agkistrodon halys pallas, Daboia (Vipera) russelli russelli, Bungarus multicinctus, Naja sp., mouse, human, bovine and cat, respectively. Phylogenetic analyses based on the amino acid sequences of 15 NGFs separate the Elapidae family (Naja and Bungarus) from those Crotalidae snakes (Bothrops, Crotalus and Agkistrodon). The three-dimensional structure of mature Bj-NGF was modeled based on the crystal structure of the human NGF. The model reveals that the core of NGF, formed by a pair of P-sheets, is highly conserved and the major mutations are both at the three beta-hairpin loops and at the reverse turn. (C) 2002 Societe francaise de biochimie et biologic moleculaire/Editions scientifiques et medicales Elsevier SAS. All rights reserved.

Sapri himalayana Griff. und ihre Beziehungen zu Richthofenia siamensis Hosseus

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An assessment of the geographical scale of recurrent gene flow in wild populations of two species of Mekong River carps (Henicorhynchus spp.)

The Mekong is the most productive river fishery in the world, and such as, the Mekong River Basin (MRB) is very important to very large human populations across the region as a source of revenue (through fishing and marketing of aquatic resources products) and as the major source for local animal protein. Threats to biodiversity in the MRB, either to the fishery sector itself or to other sectors are a major concern, even though currently, fisheries across this region are still very productive. If not managed properly however, fish population declines will cause significant economic impact and affect livelihoods of local people and will have a major impact on food security and nutrition. Biodiversity declines will undoubtedly affect food security, income and socio-economic status of people in the MRB that depend on aquatic resources. This is an indicator of unsustainable development and hence should be avoided. Genetic diversity (biodiversity) that can be measured using techniques based on DNA markers; refers to variation within and among populations within the same species or reproductive units. In a population, new genetic variation is generated by sexual recombination contributed by individuals with mutations in genes and chromosomes. Over time, populations of a species that are not reproducing together will diverge as differential impacts of selection and genetic drift change their genetic attributes. For mud carp (Henicorhynchus spp.), understanding the status of breeding units in the MRB will be important for their long term persistence, sustainability and for implementing effective management strategies. Earlier analysis of stock structure in two economically important mud carp species (Henicorhynchus siamensis and H. lobatus) in the MRB completed with mtDNA markers identified a number of populations of both species where gene flow had apparently been interrupted or reduced but applying these data directly to management unit identification is potentially compromised because information was only available about female dispersal patterns. The current study aimed to address this problem and to fully assess the extent of current gene flow (nDNA) and reproductive exchange among selected wild populations of two species of carp (Henicorhynchus spp.) of high economic importance in the MRB using combined mtDNA and nDNA markers. In combination, the data can be used to define effective management units for each species. In general, nDNA diversity for H. lobatus (with average allelic richness (A) 7.56 and average heterozygosity (Ho) 0.61) was very similar to that identified for H. siamensis (A = 6.81 and Ho = 0.75). Both mud carp species show significant but low FST estimates among populations as a result of lower genetic diversity among sampled populations compared with genetic diversity within populations that may potentially mask any 'real' population structure. Overall, population genetic structure patterns from mtDNA and nDNA in both Henicorhynchus species were largely congruent. Different population structures however, were identified for the two Henicorhynchus species across the same geographical area. Apparent co-similarity in morphology and co-distribution of these two relatively closely related species does not apparently imply parallel evolutionary histories. Differences in each species population structure likely reflect historical drainage rearrangement of the Mekong River. The data indicate that H. siamensis is likely to have occupied the Mekong system for much longer than has H. lobatus in the past. Two divergent stocks were identified for H. lobatus in the MRB below the Khone Falls while a single stock had been evident in the earlier mtDNA study. This suggests that the two Henicorhynchus species may possess different life history traits and that different patterns of gene flow has likely influenced modern genetic structure in these close congeners. In combination, results of the earlier mtDNA and the current study have implications for effective management of both Henicorhynchus species across the MRB. Currently, both species are essentially treated as a single management unit in this region. This strategy may be appropriate for H. lobatus as a single stock was evident in the main stream of the MRB, but may not be appropriate for H. siamensis as more than a single stock was identified across the same range for this species. Management strategies should consider this difference to conserve overall biodiversity (local discrete populations) and this will include maintaining natural habitat and migration pathways, provision of fish sanctuaries (refuges) and may also require close monitoring of any stock declines, a signal that may require effective recovery strategies.

Recent surveys of bats (Mammalia: Chiroptera) from China. I. Rhinolophidae and Hipposideridae

We conducted surveys of bats in China between 1999 and 2007, resulting in the identification of at least 62 species. In this paper we present data on 19 species, comprising 12 species from the family Rhinolophidae and seven from the Hipposideridae. Rhinolophids captured were Rhinolophus affinis, R. ferrumequinum, R. lepidus, R. luctus, R. macrotis, R. siamensis, R. marshalli, R. rex, R. pearsonii, R. pusillus, R. sinicus and R. stheno. Because of extensive morphological similarities we question the species distinctiveness of R. osgoodi (may be conspecific with R. lepidus), R. paradoxolophus (which may best be treated as a subspecies of R. rex), R. huananus (probably synonymous with R. siamensis), and we are skeptical as to whether R. sinicus is distinct from R. thomasi. Hipposiderids captured were Hipposideros armiger, H. cineraceus, H. larvatus, H. pomona, H. pratti, Aselliscus stoliczkanus and Coelops frithii. Of these species, two rhinolophids (Rhinolophus marshalli and R. stheno) and one hipposiderid (Hipposideros cineraceus) represent new species records for China. We present data on species' ranges, morphology and echolocation call frequencies, as well as some notes on ecology and conservation status. China hosts a considerable diversity of rhinolophid and hipposiderid bats, yet threats to their habitats and populations are substantial.

Biomassa dos principais recursos de peixe de Boa-Paz, Moçambique

Data collected during a survey conducted by the Soviet trawler Sebastopolsky rybak , in January 1987, covering the Boa-Paz area, Mozambique, were analysed. The biomass of demersal fish was found to be higher than the one of pelagic fish. The most representative group among the demersals was the first grade fish, where the families Sparidae, Lethrinidae, Lutjanidae and Serranidae were included, corresponding to a biomass of about 3,500 tonnes. In the pelagics the family Carangidae was the most important one and the species Decapterus russelli and Trachurus trachurus showed the highest biomass estimates in this family.

Genetic identification of four Malaysian mackerel species off Coast of Peninsular Malaysia based on molecular marker

Random Amplified Polymorphic DNA (RAPD) markers and cytochrome b (Cyt-b) gene sequences were utilized to fingerprint and construct phylogenetic relationships among four species of mackerel commonly found in the Straits of Malacca namely Rastrelliger kanagurta, R. brachysoma, Decapterus maruadsi and D. russelli. The UPGMA dendogram and genetic distance clearly showed that the individuals clustered into their own genus and species except for the Decapterus. These results were also supported by partial mtDNA cytochrome b gene sequences (279 bp) which found monotypic sequence for all Decapterus studied. Cytochrome b sequence phylogeny generated through Neighbor Joining (NJ) method was congruent with RAPD data. Results showed clear discrimination between both genera with average nucleotide divergence about 25.43%. This marker also demonstrated R. brachysoma and R. kanagurta as distinct species separated with average nucleotide divergence about 2.76%. However, based on BLAST analysis, this study indicated that the fish initially identified as D. maruadsi was actually D. russelli. The results highlighted the importance of genetic analysis for taxonomic validation, in addition to morphological traits.

Fishes of the Mekong River: conservation and need for aquaculture

Conservation and the potential and need for aquaculture of three major fish (Catlocarpio siamensis, Probarbus spp. and Pangasius gigas) of the Mekong River are discussed. Different steps being undertaken by the Cambodian government in doing such activities are highlighted.

Monogenean infestations in Thai silver barb (Barbodes gonionotus Bleeker) and their adaptations in Bangladesh waters

An investigation on the infestation of monogenetic trematodes of Barbodes gonionotus was conducted during the period from July '97 to June '98. Host specimens were collected from local fish farms and also from local fish markets of Mymensingh. Samples of P. ticto and P. sarana were also examined. Two species - Dactylogyrus lampam (Lim and Furtado 1986) and Dactylogyrus siamensis (Chinabut and Lim 1993) the Thai parasites were recorded from B. gonionotus and D. lampam, from our indigenous fish P. sarana. Two species of Gyrodactylus were also recorded from B. gonionotus. Both prevalence and intensity of infestation were moderate in B. gonionotus. Prevalences were recorded higher in larger fishes and mean intensity in intermediate size group fishes. Infestations were higher in winter months. Adaptations of the foreign parasites in Bangladesh waters, their transmission in local fishes and invasion of the local parasites to this exotic fish have been discussed. Suggestions have also been made to protect the introduction of new species in our waters.

Seasonal growth of five commercially important fishes at Sofala Bank, Mozambique

An analysis of growth of scad and mackerels on Sofala Bank, Mozambique, was performed using data collected from commercial vessels during the year 1986-87, using the "Compleat Elefan" software package. Updated estimates of growth parameters of Decapterus russelli, D. macrosoma (Carangidae), and Rastrelliger kanagurta (Scombridae) were obtained which take seasonal growth oscillations into account. Preliminary growth parameter estimates are also presented for Selar crumenophthalmus (Carangidae) and Saurida undosquamis (Synodontidae).

Nutritional evaluation of some small coastal fish in Sri Lanka

Small pelagic fish play a very important role in human nutrition and health. Lipids of these fish differ remarkably from plant and other animal lipids. The aim of the study was to describe the proximate composition of thirty-three small pelagic fish species commonly available in Sri Lanka. Fish species were collected from Negombo and Chillaw fish landing sites and subjected to analysis for moisture, ash, protein and total lipid content. Tiger tooth croaker (Otolithus ruber) was found to have the highest moisture percentage (80.0%) followed by Clarias sp. (78.9%), Indian anchovy (Steloporus indicus) and Comerson's anchovy (Stelophorus commersonii), (78%). The lowest percentage of moisture, 69.4%, was recorded in white sardinella (Sardinella albella). Indian ilisha (Ilisha melastoma) was found to have the highest amount of ash (10.1%) followed by Otolithus sp. (8%) and big-eye barracuda contained the least amount (2.5%). Carassius Carassius, pick handle barracuda (Sphyraena jello) and Indian mackerel (Rastrelliger kanagurta) contained higher amounts of protein, 24.3, 20.6 and 19.2% respectively. The lowest protein content (10.1%) was found in Indian scad (Decapterus russelli). The protein content of the fish was in the range of 13-15%. The results revealed that the small fish are moderate protein sources. The total lipid content varied between 0.6-8%. White sardinella recorded the highest percentage of lipid (8%) where tiger tooth croaker contained the lowest percentage (0.6 %). The study showed high fatty species to contain low amount of moisture and vice versa establishing an inverse relation between fat and moisture quantitatively.

AN ACTIVATOR OF BLOOD-COAGULATION FACTOR-X FROM THE VENOM OF BUNGARUS-FASCIATUS

A specific activator of blood coagulation factor X was purified from the venom of Bungarus fasciatus by gel filtration and by ion-exchange chromatography on a Mono-Q column (FPLC). It consisted of a single polypeptide chain, with a mel. wt of 70,000 in reducing and non-reducing conditions. The enzyme had an amidolytic activity towards the chromogenic substrates S-2266 and S-2302 but it did not hydrolyse S-2238, S2251 or S-2222, which are specific substrates for thrombin, plasmin and factor Xa, respectively. The enzyme activated factor X in vitro and the effect was Ca2+ dependent with a Hill coefficient of 7.9. As with physiological activators, the venom activator cleaves the heavy chain of factor X, producing the activated factor Xa alpha. The purified factor X activator from B. fasciatus venom did not activate prothrombin, nor did it cleave or clot purified fibrinogen. The amidolytic activity and the factor X activation activity of the factor X activator from B. fasciatus venom were readily inhibited by serine protease inhibitors such as diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), benzamidine and by soybean trypsin inhibitor but not by EDTA. These observations suggest that the factor X activator from B. fasciatus venom is a serine protease. It therefore differs from those of activators obtained from Vipera russelli and Bothrops atrox venoms, which are metalloproteinases.