Caracterización Dinámica de Partículas Magnéticas y su Uso en Aplicaciones Biomédicas

El uso de las partículas magnéticas ha venido a más durante los últimos 10 años. Lo anterior ha estado condicionado por factores como la amplia investigación en materiales a nivel atómico, los retos propuestos por las diferentes áreas de investigación donde los niveles nanométricos cobran importancia, la inherente innovación tecnológica que pueden aportar a la industria dichas escalas y finalmente, el impacto socioeconómico que pueden tener estos avances tecnológicos. Sin embargo, la traslación a la industria de los avances tecnológicos se ha visto limitada por la falta de estrategias, ya que el 88% del conocimiento se queda en Universidades y Centros de Investigación y el resto, 12%, pasa a la industria o es obtenido en ella. En este trabajo de investigación se ha tratado de completar el círculo de idea, conceptualización, generación de conocimiento, prototipo, prueba in vitro y prueba in vivo; todo ello para dejar el menor espacio posible entre Investigación e Industria. Se ha fabricado un recinto que permite la observación del comportamiento de las partículas magnéticas cuando están inmersas en un medio con viscosidad controlada y con una fuente magnética estática. De la observación experimental se han encontrado fenómenos magnéticos como la aglomeración y la autoorganización, que han sido aprovechados, no solo para ofrecer una solución alternativa a la corrección del desprendimiento de retina, sino también para promover las mejoras de métodos de separación y/o filtrado celular, ayuda en correcciones ortopédicas o en el diagnóstico y tratamiento de enfermedades degenerativas. Particularizando en la corrección del desprendimiento de retina, se ha desarrollado y patentado un Dispositivo Ocular cuya función es la de tapón del agujero retiniano, ayudando en la cicatrización de la retina. Dicho dispositivo ha sido evaluado en conejos albinos neozelandeses obteniéndose un éxito en la reaplicación de la retina del 80% y resultando su toxicidad en condición difusa tras evaluación anatomopatológica. Así mismo, el ferrofluido diseñado y fabricado se ha localizado en la zona de interacción magnética siempre y su disposición ha sido en el mayor de los casos en forma de placa pero también se han formado anillos; lo anterior no ha afectado en ningún sentido el éxito en la reaplicación de la retina. Por otro lado, no se han encontrado indicios de siderosis en la zona de interacción magnética, sus alrededores o el resto del globo ocular; ni las células han presentado apoptosis inducida por los materiales introducidos en el ojo. Sin embargo, se ha encontrado una fuerte necrosis inducida por la presión ejercida sobre la retina por el dispositivo ocular, evidenciando la necesidad de un mejor control de dicha presión. Tras la caracterización de los copos y la presión que éstos ejercen, se ha podido identificar que el dispositivo ocular se puede sintonizar para inducir una presión entre 0.5 y 2.5 mmHg; por tanto se puede vislumbrar que es posible minimizar la muerte celular causada en las células retinianas. Finalmente, tras la experimentación con conejos se ha observado que el dispositivo ocular resuelve los problemas inducidos por las siliconas o los gases e inclusive aporta ventajas competitivas de alto valor añadido como: no requerir de posiciones incómodas en el post-operatorio, no modifica las propiedades ópticas del ojo ya que no es necesario dejar silicona o gases en el interior del ojo, se pueden sellar múltiples agujeros retinianos, corrección de desprendimiento de retina inferior sin complicaciones asociadas, etc.; en definitiva mejora la calidad de vida domiciliaria del paciente además de solventar los problemas inducidos por las soluciones actuales. The using of magnetic particles has become more important during last 10 years. This event has been conditioned by several factors like a wide researching on materials at atomic level, the challenges proposed by different research areas where nanometric levels became important, the inherent technological innovation to the industry and finally, the socioeconomic impact of these kinds of technologies. However, the nanotechnological advances have not been well addressed to the industry since 88% of the acknowledge keeps on Universities and Research Centers, and the remaining 12% goes through the industry or is obtained in it. In this research work, we have tried to complete the entire process about idea, concept, acknowledge generation, prototyping and in vitro and in vivo testing; all to leave as little space as possible between Research and Industry. We have built an experimental hall which allows us the observation of magnetic particles' behavior when are immersing in a controlled viscous medium and under a static magnetic field. The experimental observation has been useful to identify and use two magnetic phenomena: agglomeration and self-organization; no only for retinal detachment, but also to improve cellular separation and/or filtered methods, orthopedic solutions or the diagnostic and treatment of degenerative diseases. Particularizing on retinal detachment, we have developed and patented an Ocular Device which its function is acting like a tampon of the retinal hole for helping with the scarring retina. The device has been evaluated in animals and the results show that we can achieve a success of 80% before rabbit’s death and non cell apoptosis, only necrosis, over the retina attached by the ocular device after anatomopathological evaluation. Also we have identified a diffuse toxicity after anatomopathological evaluation, so more experimenting must be proposed. Otherwise, the ferrofluid has been localized where the magnetic interaction area is located and its deposition has been in most cases plaque shape like but rings have also been observed; this has not affected in any way the successful reattachment of the retina. On the other hand, we have not found any evidence of siderosis in the magnetic interaction zone, near of or some other place of the ocular globe; and we did not find any apoptosis induced by the materials inserted within the eye. However full necrosis, exactly on the magnetic interaction zone, has been found and induced by the pressure exerted over the retina by the ocular device, evidencing the needing of its better control. After flakes’ characterization and its pressure exerted, we identified that the ocular device can be tuned in order to induce a pressure between 0.5 and 2.5 mmHg; therefore we can perceive that death caused to the retinal cells can be minimized. Finally, the experimentation has shown that the ocular device solves all problems induced by silicone or gases an even contributes with some other competitive advantages of high added value like: no uncomfortable postoperative positions, it does not modify any optical property of the eye because there is no needing of leaving silicone or gases inside the eye, many holes can be sealed, the inferior retinal detachment can be solve without any complications, etc.; definitively the ocular device improves patients’ quality of life.

Coastal shapes. Geentic clasification 1973 - 2010

An analysis of a stretch of coastline shows multiple alterations through environmental climate actions. The narrow, fragile line displays singularities due to three basic causes. The first is the discontinuity in feed or localised loss of solid coastal material. Called massics, their simplest examples are deltas and undersea canyons. The second is due to a brusque change in the alignment of the shoreline’s edge, headlands, groins, harbour and defence works. Given the name of geometric singularities, their simplest uses are artificial beaches in the shelter of a straight groin or spits. The third is due to littoral dynamics, emerged or submerged obstacles which diffract and refract wave action, causing a change in the sea level’s super-elevation in breaker areas. Called dynamics, the simplest examples are salients, tombolos and shells. Discussion of the causes giving rise to variations in the coastline and formation of singularities is the raison d’être of investigation, using actual cases to check the suitability of the classification proposed, the tangential or differential action of waves on the coastal landscape in addition to possible simple, compound and complex shapes detected in nature, both in erosion and deposit processes

Practical case with regard to applying the functional and environmental method to low crest level, detached breakwaters

The research work as presented in this article covers the design of detached breakwaters since they constitute a type of coastal defence work with which to combat many of the erosion problems found on beaches in a stable, sustainable fashion. The main aim of this work is to formulate a functional and environmental (but not structural) design method, enabling the fundamental characteristics of a detached breakwater to be defined as a function of the effect it is wished to induce on the coast, and taking into account variables of a different nature (climate, geomorphology and geometry) influencing the changes the shoreline undergoes after its construction. With this article, it is intended to submit the final result of the investigation undertaken, applying the detached breakwater design method as developed to solving a practical case. Thus it may be shown how the method enables a detached breakwater’s geometric pre-sizing to be tackled at a place on the coast with certain climate, geomorphology and littoral dynamic characteristics, first setting the final state of equilibrium it is wanted to obtain therein after its construction.

Methodology for the design of offshore wind farms

Although still in an early stage, offshore wind development is now characterized by a boom process. This leads to the necessity of applying an integral management model for the design of offshore wind facilities, being the purpose of the model to achieve technical, economical and environmental viability, all within a sustainable development framework. The foregoing led to the research project exposed in this paper, consisting of drawing up an offshore wind farms methodological proposal; this methodology has a global and/or general nature or point of view whilst searching for optimization of the overall process of operations leading to the design of this type of installations and establishing collated theoretical bases for the further development of management tools. This methodological proposal follows a classical engineering thought scheme: it begins with the alternatives study, and ends with the detailed design. With this in mind, the paper includes the following sections: introduction, methodology used for the research project, conditioning factors, methodological proposal for the design of offshore wind farms, checking the methodological proposal, and conclusions

Offshore Wind Farms: Foundations and influence on the Littoral Processes

Offshore wind farms are beginning to form part of coastal and marine landscapes located in dynamic surroundings. An integral management model must therefore be applied to achieve not only technical and economic viability of the project but also respect for the environment. Amongst other aspects, the latter calls for an analysis of the possible impact these facilities may have on littoral processes and this requires the differences between littoral processes prior and subsequent to the facility’s construction to be known. The maritime climate, the composition of the coast, lay-out distribution and characteristics of the facility’s components need to be known, particularly foundations as they are the main obstacles waves and currents meet. This article first addresses different aspects related to an offshore wind farm’s influence on the analysis of how it affects littoral dynamics and, because of their importance in this study, pays special attention to foundations. Coastal erosion due to this type of facility is then examined. The main conclusion of this article is that, whilst there are certain opinions claiming the coast is not affected by the presence of this kind of facility since the distance from location to coast and between wind turbine generators themselves is long, the impact must be analysed in each specific case, at least until experience proves otherwise and criteria are adopted in this respect.

Coastal Urban Flood Resislience versus Climate Change

Processes of founding and expanding cities in coastal areas have undergone great changes over time driven by environmental conditions. Coastal settlements looked for places above flood levels and away from swamps and other wetlands whenever possible. As populations grew, cities were extending trying to avoid low and wet lands. No city has been able to limit its growth. The risk of flooding can never be eliminated, but only reduced to the extent possible. Flooding of coastal areas is today dramatically attributed to eustasic sea level rise caused by global climate change. This can be inaccurate. Current climate change is generating an average sea level upward trend, but other regional and local factors result in this trend being accentuated in some places or attenuated, and even reversed, in others. Then, the intensity and frequency of coastal flooding around the planet, although not so much as a unique result of this general eustasic elevation, but rather of the superposition of marine and crustal dynamic elements, the former also climate-related, which give rise to a temporary raising in average sea level in the short term. Since the Little Ice Age the planet has been suffering a global warming change leading to sea level rise. The idea of being too obeying to anthropogenic factors may be attributed to Arrhenius (1896), though it is of much later highlight after the sixties of the last century. Never before, the human factor had been able of such an influence on climate. However, other types of changes in sea levels became apparent, resulting from vertical movements of the crust, modifications of sea basins due to continents fracturing, drifting and coming together, or to different types of climate patterns. Coastal zones are then doubly susceptible to floods. Precipitation immediately triggers pluvial flooding. If it continues upland or when snow and glaciers melt eventually fluvial flooding can occur. The urban development presence represents modifying factors. Additional interference is caused by river and waste water drainage systems. Climate also influences sea levels in coastal areas, where tides as well as the structure and dynamic of the geoid and its crust come into play. From the sea, waters can flood and break or push back berms and other coastline borders. The sea level, controlling the mouth of the main channel of the basin's drainage system, is ultimately what governs flood levels. A temporary rise in sea level acts as a dam at the mouth. Even in absence of that global change, so, floods are likely going to increase in many urban coastal areas. Some kind of innovative methodologies and practices should be needed to get more flood resilience cities

Foundations for offshoe wind farms

Análisis de las cimentaciones de los parques eólicos marinos situados en aguas de transición y en indefinidas, tipo gravedad; rozamiento, tipo pilotes; plataformas y flotantes, estudiando el fenómeno conjunto de interacción suelo - estructura y socavación

El problema del deslinde de la zona marítimo terrestre

Se aborda el origen y la realidad del demanio costero y de su deslinde respecto del resto de las tierras firmes; se reflexiona sobre la capacidad de la clasificación genética de Pedro Suarez Bores para ilustrarlo y determinarlo

Urban Coastal Flooding and Climate Change

Dentro del marco del Proyecto Europeo Smartest se presenta la publicación de las inundaciones en ciudades próximas a la costa y la influencia del cambio climático en la interface fluvio - marina con las condiciones ambientales que afectan al comportamiento evolutivo de la ciudad

Adaptabilidad al Cambio Climático: Las inundaciones y la Adaptación Urbana. El fenómeno de la inundación

Análisis del clima y el territorio, los factores oceánicos, los factores humanos, los tipos de inundaciones urbanas y sus daños, recuperación, adaptación y resiliencia

Coastal Factors and Coastal Inland. Flooding Managemenet

Siguiendo el proyecto europeo Smartest se plantea el problema de las inundaciones y su gestión en zonas urbanas en las proximidades de la costa

Evaluación de las Prestaciones de una Red WiMAX Móvil en un Escenario de Alta Movilidad

The aim of this paper is to evaluate the performance of mobile WiMAX technology for users in a highly mobility scenario for an operating frequency of 3.5 GHz. By utilizing a modified version of the extended Erceg’s propagation model, based on the introduction of Rayleigh fading due to multipath, we have calculated the received desired power and the interference power to obtain the statistical signal-to-interference-plusnoise rate (as a function of mean value and variance of cochannel interference) and the user throughput. A rural scenario composed of a transmitting base station and users in moving vehicles along a cell sector is proposed. The obtained results about coverage and throughput have been simulated by a tool built in MATLAB

Migration to an a on-line laboatory in mechanical engineering. The case of a cam-follower design practice.

The European Higher Education Area (EHEA) has leaded to a change in the way the subjects are taught. One of the more important aspects of the EHEA is to support the autonomous study of the students. Taking into account this new approach, the virtual laboratory of the subject Mechanisms of the Aeronautical studies at the Technical University of Madrid is being migrated to an on-line scheme. This virtual laboratory consist on two practices: the design of cam-follower mechanisms and the design of trains of gears. Both practices are software applications that, in the current situation, need to be installed on each computer and the students carry out the practice at the computer classroom of the school under the supervision of a teacher. During this year the design of cam-follower mechanisms practice has been moved to a web application using Java and the Google Development Toolkit. In this practice the students has to design and study the running of a cam to perform a specific displacement diagram with a selected follower taking into account that the mechanism must be able to work properly at high speed regime. The practice has maintained its objectives in the new platform but to take advantage of the new methodology and try to avoid the inconveniences that the previous version had shown. Once the new practice has been ready, a pilot study has been carried out to compare both approaches: on-line and in-lab. This paper shows the adaptation of the cam and follower practice to an on-line methodology. Both practices are described and the changes that has been done to the initial one are shown. They are compared and the weak and strong points of each one are analyzed. Finally we explain the pilot study carried out, the students impression and the results obtained.

Research lines in Hyperthermia at the Bioinstrumentation Laboratory of the Centre for Biomedical Technology

The Bioinstrumentation Laboratory belongs to the Centre for Biomedical Technology (CTB) of the Technical University of Madrid and its main objective is to provide the scientific community with devices and techniques for the characterization of micro and nanostructures and consequently finding their best biomedical applications. Hyperthermia (greek word for “overheating”) is defined as the phenomenon that occurs when a body is exposed to an energy generating source that can produce a rise in temperature (42-45ºC) for a given time [1]. Specifically, the aim of the hyperthermia methods used in The Bioinstrumentation Laboratory is the development of thermal therapies, some of these using different kinds of nanoparticles, to kill cancer cells and reduce the damage on healthy tissues. The optical hyperthermia is based on noble metal nanoparticles and laser irradiation. This kind of nanoparticles has an immense potential associated to the development of therapies for cancer on account of their Surface Plasmon Resonance (SPR) enhanced light scattering and absorption. In a short period of time, the absorbed light is converted into localized heat, so we can take advantage of these characteristics to heat up tumor cells in order to obtain the cellular death [2]. In this case, the laboratory has an optical hyperthermia device based on a continuous wave laser used to kill glioblastoma cell lines (1321N1) in the presence of gold nanorods (Figure 1a). The wavelength of the laser light is 808 nm because the penetration of the light in the tissue is deeper in the Near Infrared Region. The first optical hyperthermia results show that the laser irradiation produces cellular death in the experimental samples of glioblastoma cell lines using gold nanorods but is not able to decrease the cellular viability of cancer cells in samples without the suitable nanorods (Figure 1b) [3]. The generation of magnetic hyperthermia is performed through changes of the magnetic induction in magnetic nanoparticles (MNPs) that are embedded in viscous medium. The Figure 2 shows a schematic design of the AC induction hyperthermia device in magnetic fluids. The equipment has been manufactured at The Bioinstrumentation Laboratory. The first block implies two steps: the signal selection with frequency manipulation option from 9 KHz to 2MHz, and a linear output up to 1500W. The second block is where magnetic field is generated ( 5mm, 10 turns). Finally, the third block is a software control where the user can establish initial parameters, and also shows the temperature response of MNPs due to the magnetic field applied [4-8]. The Bioinstrumentation Laboratory in collaboration with the Mexican company MRI-DT have recently implemented a new research line on Nuclear Magnetic Resonance Hyperthermia, which is sustained on the patent US 7,423,429B2 owned by this company. This investigation is based on the use of clinical MRI equipment not only for diagnosis but for therapy [9]. This idea consists of two main facts: Magnetic Resonance Imaging can cause focal heating [10], and the differentiation in resonant frequency between healthy and cancer cells [11]. To produce only heating in cancer cells when the whole body is irradiated, it is necessary to determine the specific resonant frequency of the target, using the information contained in the spectra of the area of interest. Then, special RF pulse sequence is applied to produce fast excitation and relaxation mechanism that generates temperature increase of the tumor, causing cellular death or metabolism malfunction that stops cellular division

Analysis and optimization of propagation losses in LiNbO3 optical waveguides produced by swift heavy-ion irradiation

The propagation losses (PL) of lithium niobate optical planar waveguides fabricated by swift heavy-ion irradiation (SHI), an alternative to conventional ion implantation, have been investigated and optimized. For waveguide fabrication, congruently melting LiNbO3 substrates were irradiated with F ions at 20 MeV or 30 MeV and fluences in the range 1013–1014 cm−2. The influence of the temperature and time of post-irradiation annealing treatments has been systematically studied. Optimum propagation losses lower than 0.5 dB/cm have been obtained for both TE and TM modes, after a two-stage annealing treatment at 350 and 375∘C. Possible loss mechanisms are discussed.