Enhanced Botrytis cinerea resistance of Arabidopsis plants grown in compost may be explained by increased expression of defense-related genes, as revealed by microarray analysis

Composts are the products obtained after the aerobic degradation of different types of organic matter waste and can be used as substrates or substrate/soil amendments for plant cultivation. There is a small but increasing number of reports that suggest that foliar diseases may be reduced when using compost, rather than standard substrates, as growing medium. The purpose of this study was to examine the gene expression alteration produced by the compost to gain knowledge of the mechanisms involved in compost-induced systemic resistance. A compost from olive marc and olive tree leaves was able to induce resistance against Botrytis cinerea in Arabidopsis, unlike the standard substrate, perlite. Microarray analyses revealed that 178 genes were differently expressed, with a fold change cut-off of 1, of which 155 were up-regulated and 23 were down-regulated in compost-grown, as against perlite-grown plants. A functional enrichment study of up-regulated genes revealed that 38 Gene Ontology terms were significantly enriched. Response to stress, biotic stimulus, other organism, bacterium, fungus, chemical and abiotic stimulus, SA and ABA stimulus, oxidative stress, water, temperature and cold were significantly enriched, as were immune and defense responses, systemic acquired resistance, secondary metabolic process and oxireductase activity. Interestingly, PR1 expression, which was equally enhanced by growing the plants in compost and by B. cinerea inoculation, was further boosted in compost-grown pathogen-inoculated plants. Compost triggered a plant response that shares similarities with both systemic acquired resistance and ABA-dependent/independent abiotic stress responses.

Utilización de compost agotado de champiñón como capa de coberturas en nuevos ciclos de producción

El objetivo de este trabajo fue evaluar el comportamiento agronómico de seis mezclas de cobertura, elaboradas a partir de sustrato postcultivo del champiñón Agaricus bisporus, en nuevos ciclos de cultivo. Los resultados obtenidos mostraron la viabilidad de la reintroducción del sustrato en nuevos ciclos de cultivo, ya sea como material de base único, si se somete a un proceso de lavado para eliminar sales solubles, o bien mezclado con otros materiales de baja conductividad, como es el caso de la turba rubia o la fibra de coco. Se destacan los altos valores de eficiencia biológica registrados, que llegaron hasta los 100 kg kg-1 de compost, similares a los proporcionados por los testigos, y los altos valores relativos observados (con respecto a los testigos) en el contenido en materia seca de los carpóforos cosechados con algunas de las nuevas coberturas elaboradas. Esta reutilización del compost constituye una alternativa interesante, con vistas a reemplazar a las tierras y a los sustratos orgánicos utilizados habitualmente como cobertura, con la doble ventaja de disminuir los costos de elaboración y el impacto ambiental.

Sustratos de cobertura y suplementación del compost en cultivo de champiñón

El objetivo de este trabajo fue describir el comportamiento de la corteza de pino, de la fibra de coco y del sustrato postcultivo, como capas de cobertura, en el cultivo del champiñón (Agaricus bisporus). Tras la caracterización física, química y biológica de las capas de cobertura, se evaluaron los parámetros de producción cualitativos y cuantitativos, en un ciclo de cultivo de champiñón con cuatro floradas. La menor porosidad y capacidad de retención de agua del suelo mineral, sin aditivos, y la alta conductividad eléctrica del sustrato postcultivo del champiñón utilizado, asociado a la suplementación nutritiva, son los factores determinantes en el diferente comportamiento agronómico observado. Las mezclas de cobertura influenciaron el rendimiento y el número de champiñones, la eficiencia biológica, la tasa de producción y la precocidad. El mejor rendimiento total se obtuvo con la mezcla de suelo mineral y fibra de coco suplementado. Las capas de coberturas evaluadas pueden ser utilizadas comercialmente para la producción de champiñón.

Phosphorous and nitrogen changes in compost-amended schist soils in vineyards in the D.O. Priorat wine-producing area (NE Spain)

The impact, on nitrogen and phosphorous dynamics, of applying compost at different rates was investigated in soils developed on schist in new terraced vineyards (NTV) and in undisturbed areas (NC). Repacked soil columns amended with 0 (control), 50 t ha –1 (T1) and 100 t ha–1 (T2) of compost were studied under laboratory conditions simulating both situations. The columns were maintained for 1 year, during which time a total of 300 mm of simulated rainfall was applied in ten 30 mm applications. Soil organic matter (OM), nitrogen and phosphorous contents were analysed at the end of the study period and leachates were analysed after each simulated rainfall event. Significant differences in nitrate leaching were observed between the control and the treated soils and these differences were greater in the NC (control = 1.368 g, T1 = 1.526 g and T2 = 1.686 g) than in the NTV soils (control = 0.61 g, T1 = = 1.068 g and T2 = 1.283 g). The relative effect was greater in the NTV soils (T1/control = 1.11 vs. 1.75 and T2/control = 1.23 vs. 2.1 for NC and NTV, respectively). The nitrate concentration in the leached water reached up to 400 mg L–1, which implied a risk of groundwater pollution. Phosphorous losses through leaching were very low with concentrations of < 0.15 mg L–1, without any significant differences between treatments. The phosphorous concentrations in the surface horizon increased by 50.8% in T1 and by 66.8% in T2 in the NC soils, compared with increases of 20.3% and 38%, respectively, in the NTV soils. Due to the high infiltration capacity of the study soils, leaching effects must be considered in order to prevent groundwater pollution.

Guavira emergence and seedling production with substrates containing organic compost and soil under different screen environments

This study aimed to evaluate different proportions of organic compost and soil as a substrate for the guavira emergence and seedling formation under different protected environments, in the high Pantanal region of the state of Mato Grosso do Sul. The seeds were placed in polyethylene bags (15 x 25 centimeters) filled with four percentages of organic compost (0%, 20%, 80%, and 100% of total volume) mixed with soil. These substrates were tested in agro-nurseries covered with black screen and 50% thermo-reflecting shade cloths. The substrate with 20% soil and 80% organic compost and the black screen shade cloth promote the best performance in the seedling production.

Dry toilet compost and separated urine as fertilisers for cabbage and potato – a case study from Finland.

Konferenssijulkaisu

Effects of early compost application on no-till organic soybean

Weed control has always been an important issue in agriculture. With the advent of no-till systems, soil erosion was reduced but herbicide use was increased. Organic no-till systems try to adjust reduced erosion to the no use of herbicides. Nevertheless, this adjustment is limited by the cost of mechanical weed control. This cost may be reduced by improved cultural weed control with cover crops mulches. In this paper we report a study on the application of compost manure on an oats winter cover crop, preceding soybean, instead of on the soybean summer crop. Treatments comprised a control without compost manure, and compost manure doses of 4 and 8 Mg ha-1 applied either on oats in winter or soybean in summer, organized in a randomized block design, with five replications. In summer, plots were split into weed-controlled or not controlled subplots. The timing of application and the manure doses did not affect the oats biomass or the soybean performance. However, in summer, without water stress, the application of manure at 8 Mg ha-1 directly on soybean has reduced weed biomass in this crop.

Factors affecting farmers’ decisions on utilization of rice straw compost in Northeastern Thailand

Rice straw is used in Northeastern Thailand as an alternative to organic fertilizer for crop production. This enables farmers to reduce the use of chemical fertilizers which leads to a decrease in production costs. In spite of the beneficial effects in agricultural production, rice straw compost cannot be produced in large amounts because the burning of rice straws is a common farming practice. The decisions of farmers who use rice straw compost have been investigated by interviewing 120 households belonging to the members of an organic fertilizer user group using a household questionnaire. The study was conducted to evaluate the factors that affect the use of rice straw compost in Khon Kaen Province in Northeastern Thailand. Results of the logit model showed that the farmers’ education, number of rice straw compost trainings in which the farmer participated, lack of knowledge about technology, insufficient labour and difficulty in making rice straw compost had a significant impact on the farmer’s decision to use rice straw compost. Difficulty in making rice straw compost appeared to be the root cause because the procedure of making rice straw compost is complex and labour intensive. Repeated trainings thus, will have a positive and significant influence on farmers’ adoption of the technology. Training provides more knowledge and will presumably change the perception of the farmers towards new technologies and the awareness of positive effects of rice straw compost utilization.

Pathogens occuring in the winter pea-maize-winter wheat rotation, their host specificity and the potential of compost in suppressing foot and root disease of peas

The overall aim of the work presented was to evaluate soil health management with a specific focus on soil borne diseases of peas. For that purpose field experiments were carried out from 2009 until 2013 to assess crop performance and pathogen occurrence in the rotation winter pea-maize-winter wheat and if the application of composts can improve system performance. The winter peas were left untreated or inoculated with Phoma medicaginis, in the presence or absence of yard waste compost at rate of 5 t dry matter ha-1. A second application of compost was made to the winter wheat. Fusarium ssp. were isolated and identified from the roots of all three crops and the Ascochyta complex pathogens on peas. Bioassays were conducted under controlled conditions to assess susceptibility of two peas to Fusarium avenaceum, F. solani, P. medicaginis and Didymella pinodes and of nine plant species to F. avenaceum. Also, effects of compost applications and temperature on pea diseases were assessed. Application of composts overall stabilized crop performance but it did not lead to significant yield increases nor did it affect pathogen composition and occurrence. Phoma medicaginis was dominating the pathogen complex on peas. F. graminearum, F. culmorum, F. proliferatum, Microdochium nivale, F. crookwellense, F. sambucinum, F. oxysporum, F. avenaceum and F. equiseti were frequently isolated species from maize and winter wheat with no obvious influence of the pre-crop on the Fusarium species composition. The spring pea Santana was considerably more susceptible to the pathogens tested than the winter pea EFB33 in both sterile sand and non-sterilized field soil. F. avenaceum was the most aggressive pathogen, followed by P. medicaginis, D. pinodes, and F. solani. Aggressiveness of all pathogens was greatly reduced in non-sterile field soil. F. avenaceum caused severe symptoms on roots of all nine plant species tested. Especially susceptible were Trifolium repens, T. subterraneum, Brassica juncea and Sinapis alba in addition to peas. Reduction of growing temperatures from 19/16°C day/night to 16/12°C and 13/10°C did not affect the efficacy of compost. It reduced plant growth and slightly increased disease on EFB33 whereas the highest disease severity on Santana was observed at the highest temperature, 19/16°C. Application of 20% v/v of compost reduced disease on peas due to all four pathogens depending on pea variety, pathogen and growing media used. Suppression was also achieved with lower application rate of 3.5% v/v. Tests with γ sterilized compost suggest that the suppression of disease caused by Fusarium spp. is biological in origin, whereas chemical and physical properties of compost are playing an additional role in the suppression of disease caused by D. pinodes and P. medicaginis.

Anàlisi paramètrica de l'assaig a compressió d'un panell de material compost rigiditzat amb tres nervis

L’objectiu d’aquest treball és desenvolupar una metodologia per realitzar l’anàlisi paramètrica de l’assaig de compressió d’un panell de material compost rigiditzat amb tres nervis. En primer lloc és necessari desenvolupar un sistema automatitzat per generar i avaluar el conjunt de parametritzacions. A continuació, s’estudiaran quines variables d’estat són les més adequades per representar el vinclament local, la flexió global, la càrrega crítica de desestabilització i l’índex de fallada en l’anàlisi paramètrica. La modelització amb el mètode dels elements finits serveix per simular l’assaig a compressió del panell. La simulació es realitza mitjançant un càlcul no lineal, per estudiar la desestabilització i els fenòmens no lineals que pateix el panell. L’estudi es complementa amb una anàlisi modal i una anàlisi lineal

Com fer compost : aprofitar els fems orgànic per adobar el jardí. 'Como hacer compuesto : aprovechar los restos orgánicos para abonar el jardín'.

Material didáctico para utilizar correctamente los residuos orgánicos que pueden servir para abonar el huerto escolar o familiar. El compuesto es el resultado de la descomposición de restos de materia orgánica por microorganismos de tal manera que el resultado es una sustancia de color oscuro y aspecto y olor parecidos a la tierra de bosque. Se explican cuatro buenas razones para hacer compuesto, de qué está hecho el compuesto, el procedimiento para su realización así como sus utilidades. Concluye con un glosario.

The role of uncomposted materials, composts, manures, and compost extracts in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production - A review

This review evaluates evidence of the impact of uncomposted plant residues, composts, manures, and liquid preparations made from composts (compost extracts and teas) on pest and disease incidence and severity in agricultural and horticultural crop production. Most reports on pest control using such organic amendments relate to tropical or and climates. The majority of recent work on the use of organic amendments for prevention and control of diseases relates to container-produced plants, particularly ornamentals. However, there is growing interest in the potential for using composts to prevent and control diseases in temperate agricultural and horticultural field crops and information concerning their use and effectiveness is slowly increasing. The impact of uncomposted plant residues, composts, manures, and compost extracts/teas on pests and diseases is discussed in relation to sustainable temperate field and protected cropping systems. The factors affecting efficacy or such organic amendments in preventing and controlling pests and disease are examined and the mechanisms through which control is achieved are described.