Effect of added nitrogen on plant litter decomposition depends on initial soil carbon and nitrogen stoichiometry

Increasing organic carbon inputs to agricultural soils through the use of pastures or crop residues has been suggested as a means of restoring soil organic carbon lost via anthropogenic activities, such as land use change. However, the decomposition and retention of different plant residues in soil, and how these processes are affected by soil properties and nitrogen fertiliser application, is not fully understood. We evaluated the rate and extent of decomposition of 13C-pulse labelled plant material in response to nitrogen addition in four pasture soils of varying physico-chemical characteristics. Microbial respiration of buffel grass (Cenchrus ciliaris L.), wheat (Triticum aestivum L.) and lucerne (Medicago sativa L.) residues was monitored over 365-days. A double exponential model fitted to the data suggested that microbial respiration occurred as an early rapid and a late slow stage. A weighted three-compartment mixing model estimated the decomposition of both soluble and insoluble plant 13C (mg C kg−1 soil). Total plant material decomposition followed the alkyl C: O-alkyl C ratio of plant material, as determined by solid-state 13C nuclear magnetic resonance spectroscopy. Urea-N addition increased the decomposition of insoluble plant 13C in some soils (≤0.1% total nitrogen) but not others (0.3% total nitrogen). Principal components regression analysis indicated that 26% of the variability of plant material decomposition was explained by soil physico-chemical characteristics (P = 0.001), which was primarily described by the C:N ratio. We conclude that plant species with increasing alkyl C: O-alkyl C ratio are better retained as soil organic matter, and that the C:N stoichiometry of soils determines whether N addition leads to increases in soil organic carbon stocks.

Consumo, digestibilidade aparente de nutrientes e balanços de nitrogênio e hídrico de ovinos alimentados com silagens de cultivares de capim-búfel

The objective of this present experiment was to determine intake and apparent digestibility of dry matter and nutrients, nitrogen (NB) and water balances (WB) of sheep fed with buffel grass silages. The buffel grass cultivars used were Tanzania, Buchuma and Biloela. 21 male, castrated, crossbred Santa Ines x Non defined genotype sheep, with initial body weight of 31.8±3.16kg were used, kept in metabolic cages. The experimental design was completely randomized with seven replicates. No differences were observed in dry matter intake in g/day (919.2) and % of body weight (2.9). In general, digestibility coefficients of dry matter varied from 37.7% to 60.0%. The NB and WB were positive, with higher NB values in sheep fed with Tanzania (5.1g/day) and Biloela (3.9g/day) and higher WB in animals fed with Buchuma (1.38kg/animal/day) and Biloela (1.42kg/animal/day). All cultivars of buffel grass evaluated (Tanzania, Buchuma and Biloela) presented adequate chemical composition, where the silage of buffel grass used corresponded to 60% of the total daily water intake by sheep.

Effects of litter and fine root composition on their decomposition in a Rhodic Paleustalf under different land uses

Plant litter and fine roots are important in maintaining soil organic carbon (C) levels as well as for nutrient cycling. The decomposition of surface-placed litter and fine roots of wheat ( Triticum aestivum ), lucerne ( Medicago sativa ), buffel grass ( Cenchrus ciliaris ), and mulga ( Acacia aneura ), placed at 10-cm and 30-cm depths, was studied in the field in a Rhodic Paleustalf. After 2 years, = 60% of mulga roots and twigs remained undecomposed. The rate of decomposition varied from 4.2 year -1 for wheat roots to 0.22 year -1 for mulga twigs, which was significantly correlated with the lignin concentration of both tops and roots. Aryl+O-aryl C concentration, as measured by 13 C nuclear magnetic resonance spectroscopy, was also significantly correlated with the decomposition parameters, although with a lower R 2 value than the lignin concentration. Thus, lignin concentration provides a good predictor of litter and fine root decomposition in the field.

A comparison of the excretion rate of endogenous purine derivatives in the urine of Bos indicus and Bos taurus steers

Estimates of microbial crude protein (MCP) production by ruminants, using a method based on the excretion of purine derivatives in urine, require an estimate of the excretion of endogenous purine derivatives (PD) by the animal. Current methods allocate a single value to all cattle. An experiment was carried out to compare the endogenous PD excretion in Bos taurus and high-content B. indicus ( hereafter, B. indicus) cattle. Five Holstein - Friesian ( B. taurus) and 5 Brahman (> 75% B. indicus) steers ( mean liveweight 326 +/- 3.0 kg) were used in a fasting study. Steers were fed a low-quality buffel grass (Cenchrus ciliaris; 59.4 g crude protein/kg dry matter) hay at estimated maintenance requirements for 19 days, after which hay intake was incrementally reduced for 2 days and the steers were fasted for 7 days. The excretion of PD in urine was measured daily for the last 6 days of the fasting period and the mean represented the daily endogenous PD excretion. Excretion of endogenous PD in the urine of B. indicus steers was less than half that of the B. taurus steers ( 190 mu mol/kg W-0.75. day v. 414 mu mol/kg W-0.75. day; combined s.e. 37.2 mu mol/kg W-0.75. day; P< 0.001). It was concluded that the use of a single value for endogenous PD excretion is inappropriate for use in MCP estimations and that subspecies-specific values would improve precision.

Ecological stoichiometry controls the transformation and retention of plant-derived organic matter to humus in response to nitrogen fertilisation

Carbon (C) sequestration in soils is a means for increasing soil organic carbon (SOC) stocks and is a potential tool for climate change mitigation. One recommended management practice to increase SOC stocks is nitrogen (N) fertilisation, however examples of positive, negative or null SOC effects in response to N addition exist. We evaluated the relative importance of plant molecular structure, soil physical properties and soil ecological stoichiometry in explaining the retention of SOC with and without N addition. We tracked the transformation of 13C pulse-labelled buffel grass (Cenchrus ciliaris L.), wheat (Triticum aestivum L.) and lucerne (Medicago sativa L.) material to the <53 μm silt + clay soil organic C fraction, hereafter named “humus”, over 365-days of incubation in four contrasting agricultural soils, with and without urea-N addition. We hypothesised that: a) humus retention would be soil and litter dependent; b) humus retention would be litter independent once litter C:N ratios were standardised with urea-N addition; and c) humus retention would be improved by urea-N addition. Two and three-way factorial analysis of variance indicated that 13C humus was consistently soil and litter dependent, even when litter C:N ratios were standardised, and that the effect of urea-N addition on 13C humus was also soil and litter dependent. A boosted regression analysis of the effect of 44 plant and soil explanatory variables demonstrated that soil biological and chemical properties had the greatest relative influence on 13C humus. Regression tree analyses demonstrated that the greatest gains in 13C humus occurred in soils of relatively low total organic C, dissolved organic C and microbial biomass C (MBC), or with a combination of relatively high MBC and low C:N ratio. The greatest losses in 13C humus occurred in soils with a combination of relatively high MBC and low total N or increasing C:N ratio. We conclude that soil variables involved in soil ecological stoichiometry exert a greater relative influence on incorporating organic matter as humus compared to plant molecular structure and soil physical properties. Furthermore, we conclude that the effect of N fertilisation on humus retention is dependent upon soil ecological stoichiometry.

Avaliacao da produtividade de treze cultivares de campim buffel, na regiao semi-arida de Peranmbuco.

Para identificar as cultivares de capim buffel (Cenchrus ciliaris L.), melhor adaptadas a regiao semi-arida do Nordeste, foi conduzido um experimento no periodo de 1979/1983, no Campo Experimental de Manejo da Caatinga, do Centro de Pesquisa Agropecuaria do Tropico Semi-Arido (CPATSA-EMBRAPA), localizado em Petrolina, PE. Foram avaliadas treze cultivares de capim buffel, estabelecidas vegetativamente em parcelas de 4 m x 2,5 m. O delineamento experimental adotado foi o de blocos ao acaso com cinco repeticoes. Entre as cultivares de porte alto, destacou-se o Molopo com maiores produtividades (6.750 kg de materia seca/ha-ano), relacao colmo/folha e altura (106 cm). Comparativamente, a Biloela, que e atualmente a mais utilizada no CPATSA, apresentou menor altura (97 cm) e produtividade (5.455 kg de materia seca/ha-ano). Entre as cultivares de porte medio, observou-se a Gayndah com menor altura (77 cm) e a F1 Hibrido 171, com menor produtividade (3.745 kg de materia seca/ha-ano). Com relacao ao valor nutritivo, temos a Gayndah (12,43%) e o Molopo (9,51%), com maior e menor teor de proteina bruta, na materia seca, respectivamente.

Alternativas atuais e potenciais de suplementação alimentar de caprinos no semi-árido do Nordeste.

A caprinocultura na reglao semi-árida do Nordeste se caracteriza pela extensividade e por baixos níveis de produtividade decorrentes, principalmente, da acentuada variação estacional na oferta de forragem da caatinga, a qual constitui sua base alimentar. Existe um consenso quanto a necessidade de suplementar os animais nos períodos críticos, tanto com energia como com proteína. As alternativas atualmente disponíveis, como a palma forrageira (Opuntia ficus indica Mill), os restolhos de cultura, e o capim buffel (Cenchrus ciliaris) sao volumosos de reduzido valor energético e são mais utilizados dentro de um enfoque de sobrevivência dos animais. Os suplementos de maior valor nutritivo são de uso limitado e apresentam problemas de economicidade. As perspectivas de uso da ureia, da leucena (Leucaena leucocephala (Lam.) de Wit) e do guandu (Cajanus cajan (L.) Millsp) na suplementaçao de caprinos no semi-árido, são discutidas com base no seu potencial como fontes de nutrientes e como alternativas adaptáveis às condições agro-ecológicas e sócio-econômicas da região.

Desempenho de novilhos suplementados no periodo seco com mistura multipla a base de leucena no semi-arido brasileiro.

O estudo objetivou avaliar uma mistura multipla composta de feno de folhas de leucena (Leucaena leucocephala (Lam.) de Wit.), raspa de mandioca e uma mistura mineral, como suplemento para novilhos azebuados, no periodo seco. A mistura foi comparada com duas outras alternativas de suplementacao: a mistura sal-ureia e a silagem de leucena. Os animais dos tres grupos pastejaram, conjuntamente, em capim buffel (Cenchrus ciliaris, L.). Ao final dos 84 dias do periodo experimental, o incremento em peso vivo nos animais submetidos a mistura multipla (22,1 kg/cab) nao diferiu (P>0,05) do observado nos animais suplementados com silagem de leucena (17,7 kg/cab), mas correspondeu (P<0,05) a quase cinco vezes o observado nos animais submetidos a mistura sal-ureia (4,6 kg/cab).

Estudio de siete leguminosas de cobertura en asocio con el cultivo de pitahaya (Hylocereus undatus, Britton y Rose), como manejo de las malezas y aporte de nutrientes

Durante la época de primera de 1995, se estableció un experimento de campo en la finca Buenos Aires, localizada en el municipio de la Concepción, departamento de Masaya, con el propósito de evaluar la influencia de siete leguminosas de cobertura en asocio en el cultivo de la pitahaya (Hylocereus undatus. Britton & Rose), como manejo de las malas hierbas y aporte de nutrientes al suelo a partir de la materia orgánica incorporada. El diseño experimental utilizado en el estudio fue un Bloque Completos al azar, con cuatro repeticiones.Los tratamientos en estudio fueron: Vigna radiata, Mucuna pruriens, Phaseolus vulgaris, Dolichos lablab, Canavalia ensiformis, Cajanus cajan (semilla gris y roja), y el tratamiento testigo, el cual representa el manejo tradicional en el control de las malezas de parte del productor. Los resultados indican que las especies de malezas más dominantes fueron plantas de la familia poaceae sobresaliendo Cenchrus brownü, Digitaria ciliaris y Eleusine indica. Melampodium divaricatum de la familia Asteraceae. Mollugo verticillata de la familia Aizoaceae. Chamaesyce hirta de la familia Euphorbiaceae. Tetramerium nervosum y Elytraria imbricata de la familia Acanthaceae. Las especies descritas anteriormente fueron las más abundantes y estuvieron presentes en la diversidad. Tanto en la abundancia de malezas como en cobertura de las leguminosas los mejores resultados se presentaron en los tratamientos Dolichos lablab y Mucuna pruriens, seguido de Cajanus cajan (Gandul semilla roja y semilla gris), y Canavalia ensiformis. Por último los tratamientos Vigna radiata, Phaseolus vulgaris y el testigo, registraron la mayor abundancia de malezas. Tanto en la diversidad como en biomasa los mejores resultados se obtuvieron en Dolichos lablab y Mucuna pruriens, seguidos de Canavalia ensiformis y Cajanus cajan (Gandul semilla gris), por último se encuentran Cajanus cajan (Gandul semilla rojo), Vigna radiata, Phaseolus vulgaris y el testigo. En cuanto al aporte de nutrientes a partir de las materia orgánica los mejores resultados se presentaron en los tratamientos Vigna radiata, Dolichos lablab, Mucuna pruriens Cajanus cajan (Gandul semilla rojo y semilla gris), seguido de Canavalia ensiformis y Phaseolus vulgaris. Por último el que menor cantidad de nutrientes aportó fue el tratamiento testigo. En relación a la biomasa de las leguminosas, los mejores resultados se obtuvieron en los tratamientos Canavalia ensiformis y Cajanus cajan (Gandul semilla gris), registrando el mayor peso seco, seguido de Mucuna pruriens, Cajanus cajan (Gandul semilla roja), y Dolichos lablab. En tanto los tratamientos Phaseolus vulgaris y Vigna radiata, registraron el menor peso seco entre las leguminosas. En cuanto al mayor número y longitud de brotes (vainas) por planta, los mejores resultados se presentaron en los tratamientos Vigna radiata, Canavalia ensiformis y Dolichos lablab, seguido de Mucuna pruriens, Phaseolus vulgaris y Cajanus cajan (Gandul semilla gris y roja). En tanto el tratamiento testigo registró el menor número y longitud de brotes (vainas) por planta. Con respecto al rendimiento (Frutos 1 ha), los mejores resultados se obtuvieron en el tratamiento Dolichos lablab, seguidos de Cajanus cajan (Gandul semilla roja), Mucuna pruriens y Vigna radiata, presentaron rendimientos similares, seguidos de Cajanus cajan (Gandul semilla gris), Canavalia ensiformis y Phaseolus vulgaris, en tanto el testigo presentó el más bajo rendimiento. En cuanto al análisis económico de los tratamientos en estudio, el de mejor rentabilidad resultó ser Dolichos lablab, dado que ofrece el más alto beneficio neto, pese a ser uno de los tratamientos en que los costos fueron los más altos, además en éste tratamiento se obtuvo el mejor rendimiento de frutos.

Estimation of chlorophyll degradation into phaeophytinin Anaptychia ciliaris as a method to detect air pollution

Se estudia la influencia de los siguientes factores sobre el valor del cociente de feofitinización DO 435 nm/DO 415 nm en el líquen Anaptychia ciliaris (L.) Kórber: edad del líquen, concentración final de pigmentos, trasplante de los líquenes a áreas distintas sobre sustratos diferentes o similares y el valor de pH del sustrato original o el adoptado tras el trasplante. Se discute la utilización de A. ciliaris como un bioindicador de la presencia de factorcs acidificantes en la atmósfera o en el agua de lluvia que provoquen la feofitinización de las clorofilas.

Tolerância de Digitaria ciliaris, Digitaria horizontalis e Digitaria nuda à herbicidas usados em cana-de-açúcar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Um modelo espaço-temporal aplicado à agricultura de precisão

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Herbicidas inibidores da ACCase em plantas de Cenchrus echinatus em estresse hídrico

This project aimed to relate the efficiency of control of ACCase inhibiting herbicides applied post-emergence in Cenchrus echinatus under different soil water contents. The experiments were conducted in a greenhouse, with the application of three different herbicides (fluazifop-p-butyl, haloxyfop-methyl and sethoxydim + oil Assist) and the experimental design for each herbicide was completely randomized design with four replications, consisting a 3 x 4 factorial, with the combination of water management strategies (-0.03, -0.07 and -1.5 MPa) and four doses of these products (100, 50, 25 and 0% of the recommended dose). Herbicide application was made at vegetative stage of 2-3 tillers. The water management strategies were initiated in the development stage of two leaves, replacing the water until the soil reaches the potential of -0.01 MPa, when it came to severe pre-determined for each water management. The physiological parameters evaluated were: photosynthetic rate, stomatal conductance, transpiration, leaf temperature and plant dry matter. The visual assessments of phytotoxicity were performed at 7, 14, 21 and 28 days after application. The efficiency of these herbicides was influenced by soil management and water lowest in plants grown in the minimal potential of water in the soil of -1.5 MPa. All the herbicides were unsatisfactory controls in applications late (2-3 tiller plants).

Growth of the crabgrass species Digitaria ciliaris and Digitaria nuda

The aim of this research paper was to compare the growth of D. ciliaris and D. nuda crabgrass species under non-competitive conditions. To this end, two experiments were conducted, one from March - July 2010 and the other from February - June 2011. The experimental design of both trials was completely randomized making a factorial (2 seasons x 2 species crabgrass x 12 evaluation periods) with four replications. Assessments began at 15 days after sowing (DAS), and repeated weekly until 92 DAS. The variables evaluated were total dry matter (roots+leaves+stems), leaf area, leaf number and tiller. The results were submitted to analysis of variance and the absolute growth rate, relative growth rate and leaf area ratio were calculated using the means, which were adjusted regression models. The crabgrass species were significantly different in leaf area, leaf number, tiller number and dry matter per plant. D. ciliaris for all variables was statistically higher than D. nuda. Regarding the speed at which the growth of the species occurred, the absolute growth rate and relative growth rate of D. ciliaris was also greater than D. nuda. In addition, D. ciliaris also had a lower leaf area ratio indicating greater efficiency in converting light energy into carbohydrates. It can be concluded that D. ciliaris has a higher growth rate in conditions where there is no limitation of nutrients and water availability in relation to D. nuda, mainly due to D. ciliaris have greater leaf area, number of leaves and dry matter accumulation per plant.

Exploring symbiont fidelity among lichen symbioses and the common alga Trebouxia decolorans s.l.: differents levels of intrathalline photobiont diversity in Xanthoria parietina and Anaptychia ciliaris

Francesco Dal Grande (Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft fuer Naturforschung, Frankfurt am Main, Germany Goethe Universitat, Frankfurt am Main, Germany). Comunicación ofrecida por David Alors Rodríguez (Universidad Complutense de Madrid)