Occurrence and significance of ovarian and follicular regression in cachara Pseudoplatystoma fasciatum (Linnaeus, 1766): a histology approach

Estudaram-se os processos de regressão ovariana e atresia folicular em cachara, Pseudoplatystoma fasciatum, mantida em cativeiro, na reprodução não induzida por hormônios. As características macro e microscópicas (diâmetro dos ovócitos e histologia) dos ovários foram descritas a cada 20 dias, em quatro estádios: na regressão inicial (Rg I - os primeiros 20 dias), na regressão intermediária (Rg II - do 21º ao 40º dia), na regressão final (Rg III - do 41º ao 80º dia) e na fase de recuperação ou de repouso II (R II - do 81º ao 150º dia). O experimento foi realizado do final de janeiro (verão-dias longos) a maio (outono-dias curtos). No início do experimento, as amostras apresentaram ovócitos com diâmetros que variaram de 437,5 a 1.187,5mm, sugerindo encontrarem-se nas fases perinucleolar, de maturação final e atrésicos. Aos 150 dias, os diâmetros atingiram os menores valores e pôde-se visualizar a zona radiata rompida e o vitelo reabsorvido. Concomitantemente, houve diminuição abrupta dos valores médios do índice gonadossomático, da temperatura da água, das horas de luz e de chuva. A involução gradual do longo processo foi dinâmica e complexa, afetando o êxito da desova (taxas de fertilização, de eclosão e de sobrevivência de larvas) e, conseqüentemente, o sistema produtivo.

The ovarian cycle histochemistry and its relationship with hepatopancreas weight in the blue crab Callinectes danae (Crustacea: Portunidae)

Zara, F.J., Gaeta, H.H., Costa, T.M., Toyama, M.H. and Caetano, F.H. 2011. The ovarian cycle histochemistry and its relationship with hepatopancreas weight in the blue crab Callinectes danae (Crustacea: Portunidae). -Acta Zoologica (Stockholm) 00:1-13. Several studies use macroscopic patterns of the ovarian development in crustaceans. Here, we examined the relationship between ovary histochemistry, changes in gonadosomatic and hepatosomatic indices against the macroscopic pattern of the ovarian development in Callinectes danae. Animals were collected in the south coast of São Paulo State, Brazil. Ovaries were macroscopically classified as juvenile, rudimentary, developing, intermediary, mature, and rudimentary ovigerous. Samples were fixed in 4% paraformaldehyde, processed for historesin, and stained with HE, protein, and neutral and acid polysaccharides detection. The juvenile oocytes are not enclosed by follicular cells and have fewer yolk nuclei being less intense in PAS reactivity than rudimentary oocytes. Developing oocytes show yolk granules and thick follicular cells. Yolk granules were positive for proteins and neutral polysaccharides. The intermediary stage is marked by a qualitative increase in yolk granules and the onset of chorion formation. In mature oocytes, cytoplasm is completely filled by yolk granules and the chorion is completely formed. Ovigerous ovaries have several atretic follicles and large quantities of hemocytes in the process of tissue reorganization. In C. danae, the changes in cell, goandosomatic and hepatosomatic indices coinciding with macroscopic observations and any combination of different macroscopic stages in a single pattern should be avoided. © 2011 The Royal Swedish Academy of Sciences.

Caracaterização ultrassonográfica, morfofisiológica do sistema reprodutor de machos e fêmeas de Crotalus durissus terrificus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos histológicos do útero, tuba uterina e ovário de búfalas (Bubalus bubalis)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expressão dos fatores de crescimento fibroblástico 17 e 18 (FGF-17 e FGF-18) em folículos antrais bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expressão e função de membrso das subfamílias FGF-8 e FGF-9 em folículos antrais bovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fatores homólogos aos fatores de crescimento fibroblásticos são expressos em folículos antrais e corpos lúteos bovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expressão gênica dos fatores de crescimento fibroblástico 7 e 10 (FGF-7 e FGF-10) ao longo do desenvolvimento folicular ovariano bovino

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Regulação da expressão dos receptores de fatores de crescimento fibroblásticos 3c e 4 (FGFR-3c e FGFR-4) em folículos antrais bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expression of receptors for BMP15 is differentially regulated in dominant and subordinate follicles during follicle deviation in cattle

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influence of Parity and Season of the Year on Oocyte Quality and Number in Buffaloes

The aim of the present study was to evaluate the effects of season of the year (summer and winter) and parity (heifers and cows) on oocyte quality and number in buffaloes. For this purpose, 71 buffaloes had follicular wave emergence synchronized before OPU. OPU of all follicles >= 2mm was done 5 days after the beginning of the hormonal protocol, in 4 replicates (two for each season). Data were analyzed by ANOVA using PROC GLIMMIX, in a 2 x 2 factorial arrangement of treatments. No interactions were observed in following variables: number of follicles, number of total and viable oocytes, recovery rate, percentage of viable oocytes, grade I oocytes, grade II oocytes, grade III oocytes, denuded oocytes, expanded cumulus oocytes, and atretic/degenerated oocytes. Number of follicles visualized at OPU and recovery rate were not affected by parity or season. Relative to parity, number of total and viable oocytes were greater in heifers than in cows, respectively. Concerning season of the year, number of viable oocytes and viable oocyte rate were increased in winter. In conclusion, better oocyte quality can be obtained from heifers and during winter in buffaloes. However, the number of total oocytes seems to be more influenced by parity than by season of the year in this species.

Microscopic Evidence of the Opportunistic Reproductive Strategy and Early Sexual Maturation of the Small-Sized Characin Knodus moenkhausii (Characidae, Pisces)

We investigated the reproductive biology of Knodus moenkhausii, an abundantsmall-sized characin fish with broad occurrence in the Parana River basin, Bra-zil. Specimens were collected monthly to determine fecundity, length at firstmaturity, reproductive period and spawning type. Gonads were macroscopically classified according to their form, size and texture in three different stages(immature, maturing or mature). Histological procedures were conducted toconfirm gonadal developmental stages, and it was possible to notice thatmaturing females actually presented atretic oocytes, and all males that weremacroscopically classified as immature, maturing and mature actually presentedabundant spermatozoa in their gonads. Because of these discrepancies, a reclas-sification of gonadal maturations stages was needed after histological analysis,reinforcing its importance to studies on the reproduction of small characins.Reproduction occurred throughout the year though with two peaks. The lengthof the smallest mature individuals was 13 mm SL for males and 24 mm SL for females. Despite presenting relatively small batch fecundity, some life historytraits such as early reproduction, multiple spawning throughout the year, inassociation with known opportunistic feeding habits, explain the high abun-dance of this species in locations where it occurs.

Controle do crescimento e da seleção folicular por fatores locais e sistêmicos na espécie bovina

The regulation of ovarian activity is an integrated process involving extraovarian signals and intrafollicular factors. In such process, gonadotropins have an important role, but not always crucial. The initiation of primordial follicle growth and the early stages of folliculogenesis can occur without gonadotropins, however FSH may affect the rate of preantral follicle growth. On the other hand, the antral follicle development from 1 to 4 mm in diameter is completely gonadotropin-dependent in bovine, and the alternation of dependency from FSH to LH may be part of the mechanism involved in follicular selection. Locally produced growth factors, such as the IGF system, work in association with gonadotropins throughout the follicular growth and can influence follicular selection. Thus, the integration of these extraovarian signals and intrafollicular factors seems to determine whether a follicle will continue to develop or go atretic. This review focuses on the interaction between gonadotropins and intrafollicular factors in follicular development and selection in cattle.

Análise hormonal, imunolocalização e quantificação dos receptores de andrógenos (AR) e estrógenos (ER-α E ER-β) em ovário e útero de ratas submetidas a diferentes doses de decanoato de nandrolona: avaliação nos períodos pós-tratamento e pós-recuperação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morfologia do útero e ovários de Artibeus planirostris (Phyllostomidae: Chiroptera) durante as fases do ciclo reprodutivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)