Materiales : la Revolución Industrial.

Este artículo pertence a un dossier monográfico titulado 'la Revolución Industrial'

Jüdische Apologetik

Moritz Güdemann

Judentaufen im neunzehnten Jahrhundert : mit besonderer Berücks. Preußens

dargest. von N. Samter

Philo und Josephus als Apologeten des Judentums

von Paul Krüger

Żydzi Lwowscy na przełomie XVIgo i XVIIgo wieku : praca odznaczona pierwszą nagrodą na konkursie im. H. Wawelberga przez wydział filozoficzny C. K. Uniwersytetu Lwowskiego

Majer Bałaban

Gründungsgeschichte des Stadt-Tempels der israelitischen Kultusgemeinde Wien. Mit e. Einl.: Die zeitgeschichtlichen allgemeinen Verhältnisse der Wiener Juden

nach archiv. Quellen von Sigmund Husserl

Das Alte Testament und die Kritik oder die Hauptprobleme der alttestamentlichen Forschung in gemeinfasslicher Weise erörtert

von Joh. Conrad Gasser

Kritische Beiträge zur Entstehungsgeschichte des Christentums

von B. Kellermann

Einige Vorschriften für Pesach

Gerson Posen

A Role for Tlg1p in the Transport of Proteins within the Golgi Apparatus of Saccharomyces cerevisiae

Members of the syntaxin protein family participate in the docking–fusion step of several intracellular vesicular transport events. Tlg1p has been identified as a nonessential protein required for efficient endocytosis as well as the maintenance of normal levels of trans-Golgi network proteins. In this study we independently describe Tlg1p as an essential protein required for cell viability. Depletion of Tlg1p in vivo causes a defect in the transport of the vacuolar protein carboxypeptidase Y through the early Golgi. Temperature-sensitive (ts) mutants of Tlg1p also accumulate the endoplasmic reticulum/cis-Golgi form of carboxypeptidase Y at the nonpermissive temperature (38°C) and exhibit underglycosylation of secreted invertase. Overexpression of Tlg1p complements the growth defect of vti1-11 at the nonpermissive temperature, whereas incomplete complementation was observed with vti1-1, further suggesting a role for Tlg1p in the Golgi apparatus. Overexpression of Sed5p decreases the viability of tlg1 ts mutants compared with wild-type cells, suggesting that tlg1 ts mutants are more susceptible to elevated levels of Sed5p. Tlg1p is able to bind His6-tagged Sec17p (yeast α-SNAP) in a dose-dependent manner and enters into a SNARE complex with Vti1p, Tlg2p, and Vps45p. Morphological analyses by electron microscopy reveal that cells depleted of Tlg1p or tlg1 ts mutants incubated at the restrictive temperature accumulate 40- to 50-nm vesicles and experience fragmentation of the vacuole.