The taming of a shrew <1596>

Contains also a facsimile of the title-page of the first edition, 1594.

The two merry milke-maids,

Interleaved.

The valiant Welshman /

Interleaved.

Everie Woman in her Humor.

Original title: Everie Woman in her Humor. London, Printed by E. A. for Thomas Archer...1609.

The honest lawyer,

"S. S. has not yet been definitely identified."--Pref.

The Famovs Victories of Henry the fifth.: Containing the Honourable Battell of Agin-court: As it was plaide by the Queenes Maiesties Players.

"One of the s̕ources ̕of Shakespeare's Henry IV, probably written about 1587."--Hand list to Old English plays.

King Richard the Third

Mode of access: Internet.

The Downfall of Robert, Earle of Huntington, Afterward Called Robin Hood of merrie Sherwodde: with his loue to chaste Matilda, the Lord Fitzwaters daughter, afterwardes his faire Maide Marian.

Label pasted on front end-paper reads: ... The downfall of Robert E. of Huntington, by Anthony Munday. Date of only known original edition, 1601 Staged, 1598-9. Reproduced in facsimile [Tudor facsimile texts] 1913.

The tragicall history of D. Faustus /

Interleaved.

The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA

It has been recognised for some time that a full code of amino acid-based recognition of DNA sequences would be useful. Several approaches, which utilise small DNA binding motifs called zinc fingers, are presently employed. None of the current approaches successfully combine a combinatorial approach to the elucidation of a code with a single stage high throughput screening assay. The work outlined here describes the development of a model system for the study of DNA protein interactions and the development of a high throughput assay for detection of such interactions. A zinc finger protein was designed which will bind with high affinity and specificity to a known DNA sequence. For future work it is possible to mutate the region of the zinc finger responsible for the specificity of binding, in order to observe the effect on the DNA / protein interactions. The zinc finger protein was initially synthesised as a His tagged product. It was not possible however to develop a high throughput assay using the His tagged zinc finger protein. The gene encoding the zinc finger protein was altered and the protein synthesised as a Glutathione S-Transferase (GST) fusion product. A successful assay was developed using the GST protein and Scintillation Proximity Assay technology (Amersham Pharmacia Biotech). The scintillation proximity assay is a dynamic assay that allows the DNA protein interactions to be studied in "real time". This assay not only provides a high throughput method of screening zinc finger proteins for potential ligands but also allows the effect of addition of reagents or competitor ligands to be monitored.