RESPONSES OF INDICATOR AND PATHOGENIC BACTERIA TO THE PRESENCE OF CARBARYL IN WATER

Biodegradability is a desirable, if not a necessary characteristic of pesticides. Carbaryl, as Sevin, is one of the more widely used insecticides for the control of agricultural pests and has been reported to be readily degraded by microorganisms. Because of its broad application, the concentration of Sevin in surface waters has been reported to reach nearly four parts per million (PPM) in surface waters, where it has been reported to affect the growth and metabolic rates of aquatic bacterial populations. Following these reports, it is of public health importance to determine the effects of this insecticide on the growth and metabolic rates of bacteria used to indicate water pollution, and on pathogenic organisms which are found in polluted water.^ This study was conducted to determine the effect of carbaryl on the growth and metabolic rates of indicator and pathogenic organisms. Escherichia coli and Streptococcus faecalis were used as indicators, while Staphylococcus aureus and Salmonella typhimurium were the pathogens studied. Pure and mixed cultures of these organisms were exposed to two concentrations of carbaryl (Sevin).^ The study demonstrated that the fecal pollution indicator organisms, E. coli and S. faecalis respond differently to the presence of small concentrations of carbaryl in water as do the two pathogens tested, (S. typhimurium and S. aureus). The growth of all test organisms as measured by spread plate counts, was reduced by the presence of either one mg/l or five mg/l carbaryl within a period of eight days. Survival of the organisms in the presence of five mg/l carbaryl varied dependent upon whether the organism was in pure or mixed culture. In the presence of five mg/l carbaryl, both pure and mixed culture of E. coli showed longer survival. S. faecalis survived for more than eight days in pure culture, neither S. typhimurium nor S. aureus survived for eight days in pure culture.^ The metabolic rate of S. faecalis and S. aureus was reduced by both five mg/l and one mg/l Sevin concentrations, contrary to E. coli and S. typhimurium which had reduced metabolic rate with the introduction of five mg/l Sevin but showed an increase in the metabolic rate with one mg/l Sevin. There was no difference between the test and control when mixed populations were exposed to five mg/l Sevin and the metabolic rate tested. A mixture of E. coli and S. typhimurium populations showed a respiration increase over the control when exposed to one mg/l Sevin concentration. If similar effects occur in polluted surface waters, misleading results from bacteriological water quality testing may occur. ^

Tab. 1: Mass changes and balances 1982/83 for stakes on the Inland Ice at Päkitsup ilordlia north-east of Jakobshavn

In connection with hydropower investigations in West Greenland mass balance measurements have been carried out 1982/83 on the Inland lce at Päkitsup ilordlia north-east of Jakobshavn. The mass balance was measured at seven stakes drilled into the ice at altitudinal intervals of 200 metres from 300 m to 1500 m a.s.l. The measurements show that mass balance conditions in the Jakobshavn area must have been abnormally positive for the 1982/83 hydrological year.

Table 1: Basic mass-balance, Peyto Glacier, 1967/1974

The eight-year record of mass balance of Peyto Glacier is correlated to meteorological data measured near the glacier and at Lake Louise 30 km to the south. The period investigated includes the llighest and lowest accumulations for the past 40 years. The primar'y controls of net annual balance are seen to be the depth of the 'winter sno,y pack and the temperature record during the summer. Extensive summer snowfalls in the ablation area can slow down melt rates very considerably and affect the net annual balance positively. The variable nature of winter accumulation and its influence on snowline retreat and ice melt is illustrated by three years' data.

Volume changes of Jacobshavn Isbrae between 1985, 1997 and 2007

Following three decades of relative stability, Jakobshavn Isbrae, West Greenland, underwent dramatic thinning, retreat and speed-up starting in 1998. To assess the amount of ice loss, we analyzed 1985 aerial photos and derived a 40 m grid digital elevation model (DEM). We also obtained a 2007 40 m grid SPOT DEM covering the same region. Comparison of the two DEMs over an area of ~4000 km**2 revealed a total ice loss of 160 ± 4 km**3, with 107 ± 0.2 km**3 in grounded regions (0.27 mm eustatic sea-level rise) and 53 ± 4 km**3 from the disintegration of the floating tongue. Comparison of the DEMs with 1997 NASA Airborne Topographic Mapper data indicates that this ice loss essentially occurred after 1997, with +0.7 ± 5.6 km**3 between 1985 and 1997 and -160 ± 7 km**3 between 1997 and 2007. The latter is equivalent to an average specific mass balance of -3.7 ± 0.2 m/a over the study area. Previously reported thickening of the main glacier during the early 1990s was accompanied by similar-magnitude thinning outside the areas of fast flow, indicating that the land-based ice continued reacting to longer-term climate forcing.

Seawater carbonate chemistry and accumulation of radiotracers in squid, Loligo vulgaris during experiments, 2011

The anthropogenic release of carbon dioxide (CO2) into the atmosphere leads to an increase in the CO2 partial pressure (pCO2) in the ocean, which may reach 950 ?atm by the end of the 21st century. The resulting hypercapnia (high pCO2) and decreasing pH ("ocean acidification") are expected to have appreciable effects on water-breathing organisms, especially on their early-life stages. For organisms like squid that lay their eggs in coastal areas where the embryo and then paralarva are also exposed to metal contamination, there is a need for information on how ocean acidification may influence trace element bioaccumulation during their development. In this study, we investigated the effects of enhanced levels of pCO2 (380, 850 and 1500 ?atm corresponding to pHT of 8.1, 7.85 and 7.60) on the accumulation of dissolved 110mAg, 109Cd, 57Co, 203Hg, 54Mn and 65Zn radiotracers in the whole egg strand and in the different compartments of the egg of Loligo vulgaris during the embryonic development and also in hatchlings during their first days of paralarval life. Retention properties of the eggshell for 110mAg, 203Hg and 65Zn were affected by the pCO2 treatments. In the embryo, increasing seawater pCO2 enhanced the uptake of both 110mAg and 65Zn while 203Hg showed a minimum concentration factor (CF) at the intermediate pCO2. 65Zn incorporation in statoliths also increased with increasing pCO2. Conversely, uptake of 109Cd and 54Mn in the embryo decreased as a function of increasing pCO2. Only the accumulation of 57Co in embryos was not affected by increasing pCO2. In paralarvae, the CF of 110mAg increased with increasing pCO2, whereas the 57Co CF was reduced at the highest pCO2 and 203Hg showed a maximal uptake rate at the intermediate pCO2. 54Mn and 65Zn accumulation in paralarvae were not significantly modified by hypercapnic conditions. Our results suggest a combined effect of pH on the adsorption and protective properties of the eggshell and of hypercapnia on the metabolism of embryo and paralarvae, both causing changes to the accumulation of metals in the tissues of L. vulgaris.

Surface mass balance along the K-transect in West Greenland

A 21-year record is presented of surface mass balance measurements along the K-transect. The series covers the period 1990-2011. Data are available at 8 sites along a transect over an altitude range of 390 - 1850 m at approximately 67° N in West Greenland. The surface mass balance gradient is on average 3.8 x 10**-3 m w.e./m, and the mean equilibrium line altitude is 1553 m a.s.l. Only the lower 3 sites within 10 km of the margin experience a significant increasing trend in the ablation over the entire period.

Determination of tricyclazole in water using solid phase extraction and liquid chromatography

A method for determination of tricyclazole in water using solid phase extraction and high performance liquid chromatography (HPLC) with UV detection at 230nm and a mobile phase of acetonitrile:water (20:80, v/v) was developed. A performance comparison between two types of solid phase sorbents, the C18 sorbent of Supelclean ENVI-18 cartridge and the styrene-divinyl benzene copolymer sorbent of Sep-Pak PS2-Plus cartridge was conducted. The Sep-Pak PS2-Plus cartridges were found more suitable for extracting tricyclazole from water samples than the Supelclean ENVI-18 cartridges. For this cartridge, both methanol and ethyl acetate produced good results. The method was validated with good linearity and with a limit of detection of 0.008gL-1 for a 500-fold concentration through the SPE procedure. The recoveries of the method were stable at 80% and the precision was from 1.1-6.0% within the range of fortified concentrations. The validated method was also applied to measure the concentrations of tricyclazole in real paddy water.

Catalytic current polarographic determination of traces of titanium in water after preconcentration by coflotation

A new method is reported for the determination of trace levels of Ti(IV) in water by coflotation and polargraphy. Ti(IV) is preconcentrated and separated by coflotation using aluminium hydroxide as coprecipitant and sodium oleate as surfactant. Polarographic determination of titanium content in the froth is based on the catalytic wave of Ti(IV) in the presence of chlorate and oxalate. The effect of various cations and anions on the flotation and determination of titanium has been investigated. The method has been applied to estimation of titanium in natural fresh water samples.

Sulfur transfer reactions of tetrathiomolybdate in water: Synthesis of alkyl disulfides from alkyl halides

Reaction of a number of alkyl halides with tetrathiomolybdate in water as the solvent affords the corresponding disulfides in good yields.