Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells

In rabbit ligated ileal loops, two atypical enteropathogenic Escherichia coli (aEPEC) strains, 3991-1 and 0421-1, intimately associated with the cell membrane, forming the characteristic EPEC attachment and effacement lesion of the brush border, induced a mucous hypersecretion, whereas typical EPEC (tEPEC) strain E2348/69 did not. Using cultured human mucin-secreting intestinal HT29-MTX cells, we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. The transcription of the MUC5AC and MUC4 genes was transiently upregulated after aEPEC infection. We provide evidence that the apically adhering aEPEC cells exploit the mucins` increased production since they grew in the presence of membrane-bound mucins, whereas tEPEC did not. The data described herein report a putative new virulence phenomenon in aEPEC.

The politicks on both sides, with regard to foreign affairs, stated from their own writings, and examined by the course of events. : With some observations on the present state of affairs in Great Britain, and the effects of our negotiations, for several years past.

Head-piece; initial.

History for ready reference, from the best historians, biographers, and specialists; their own words in a complete system of history ...

Paged continuously.

The Blackwater chronicle : a narrative of an expedition into the land of Canaan, in Randolph county, Virginia, a country flowing with wild animals, such as panthers, bears, wolves, elk, deer, otter, badger, &c., &c., with innumerable trout--by five adventurous gentlemen, without any aid of government, and solely by their own resources, in the summer of 1851 /

Generally attributed to John Pendleton Kennedy, brother of the author. cf. H.T. Tuckerman, Life of J.P. Kennedy, 1871, p. 26 and 421.

A new and improved Spanish grammar, designed for every class of learners, but especially for such as are their own instructors. In two parts: Part I. An easy introduction to the elements of the Spanish language. Part II. The rules of etymology and syntax fully exemplified, with occasional notes and observations.

Mode of access: Internet.

The new Hamlet, intermixed and interwoven with a revised version of Romeo and Juliet; the combination being modernized, rewritten and wrought out on new-discovered lines, as indicated under the light of the higher criticism by Wm. Hawley Smith and the Smith family, farmers. Printed from the original manuscript, with text in full, and as first produced when done in action by the Smiths, their own company, under the haw tree, on their farm, at the ticket, June 17, 1902.

"Done into a book, and bound in boards (no. 2 fencing) by and, at the barn, on the farm, by farmers."

The illustrated strawberry culturist : containing the history, sexuality, field and garden culture of strawberries, forcing or pot culture, how to grow from seed, hybridizing, and all other information neccessary to enable everybody to raise their own strawberries, together with a description of new varieties and a list of the best of the old sorts -- /

Includes index.

Domestic medicine; or, The family physician : being an attempt to render the medical art more generally useful, by shewing people what is in their own power both with respect to the prevention and cure of diseases. Chiefly calculated to recommend a proper attention to regimen and simple medicines.

Signatures: [a]-c⁴, B-Z⁴, Aa-Zz⁴, Aaa-Nnn⁴ (Nnn₄ blank).