Genes up- and down-regulated by dermcidin in breast cancer: a microarray analysis

Dermcidin (DCD) is a human gene mapped to chromosome 12q13 region, which is co-amplified with multiple oncogenes with a well-established role in the growth, survival and progression of breast cancers. Here, we present a summary of a DNA microarray-based study that identified the genes that are up- and down-regulated in a human MDA-361 pLKO control clone and three clones expressing short hairpin RNA against three different regions of DCD mRNA. A list of 235 genes was differentially expressed among independent clones (> 3-fold change and P < 0.005). The gene expression of 208 was reduced and of 27 was increased in the three DCD-RNAi clones compared to pLKO control clone. The expression of 77 genes (37%) encoding for enzymes involved in amino acid metabolism, glucose metabolism and oxidoreductase activity and several genes required for cell survival and DNA repair were decreased. The expression of EGFR/ErbB-1 gene, an important predictor of outcome in breast cancer, was reduced together with the genes for betacellulin and amphiregulin, two known ligands of EGFR/ErbB receptors. Many of the 27 genes up-regulated by DCD-RNAi expression have not yet been fully characterized; among those with known function, we identified the calcium-calmodulin-dependent protein kinase-II delta and calcineurin A alpha. We compared 132 up-regulated and 12 down-regulated genes in our dataset with those genes up- and down-regulated by inhibitors targeting various signaling pathway components. The analysis showed that the genes in the DCD pathway are aligned with those functionally influenced by the drugs sirolimus, LY-294002 and wortmannin. Therefore, DCD may exert its function by activating the PI3K/AKT/mTOR signaling pathway. Together, these bioinformatic approaches suggest the involvement of DCD in the regulation of genes for breast cancer cell metabolism, proliferation and survival.

The rating of perceived exertion predicts intermittent vertical jump demand and performance

The aims of this study were (a) to assess the ability of the rating of perceived exertion (RPE) to predict performance (i.e. number of vertical jumps performed to a fixed jump height) of an intermittent vertical jump exercise, and (b) to determine the ability of RPE to describe the physiological demand of such exercise. Eight healthy men performed intermittent vertical jumps with rest periods of 4, 5, and 6s until fatigue. Heart rate and RPE were recorded every five jumps throughout the sessions. The number of vertical jumps performed was also recorded. Random coefficient growth curve analysis identified relationships between the number of vertical jumps and both RPE and heart rate for which there were similar slopes. In addition, there were no differences between individual slopes and the mean slope for either RPE or heart rate. Moreover, RPE and number of jumps were highly correlated throughout all sessions (r=0.97-0.99; P0.001), as were RPE and heart rate (r=0.93-0.97; P0.001). The findings suggest that RPE can both predict the performance of intermittent vertical jump exercise and describe the physiological demands of such exercise.

Volatile organic compounds produced by Saccharomyces cerevisiae inhibit the in vitro development of Guignardia citricarpa, the causal agent of citrus black spot

Due to the low chemical control effectiveness of citrus black spot, caused by the fungus Guignardia citricarpa at postharvest, and to the search for alternative control methods, this study aimed to evaluate the in vitro effect of volatile organic compounds (VOCs), produced by yeast Saccharomyces cerevisiae, on G. citricarpa. It was observed that the yeast strains evaluated acted as antagonists by VOC production, whose maximum inhibitory capacity was as high as 87.2%. The presence of fermentable carbon sources in the medium was essential for the bioactive VOC production by the yeast. The analysis of VOCs produced in PDA medium by SPME-GC-MS indicated the presence of high quantities of alcohols as well as esters. An artificial VOC mixture prepared on the basis of the composition of the VOCs mimicked the inhibitory effects of the natural VOCs released by S. cerevisiae. Thus, the VOCs produced by the yeast or the artificial mixtures can be a promising control method for citrus black spot or others postharvest diseases.

Association of mannose-binding lectin gene polymorphism but not of mannose-binding serine protease 2 with chronic severe aortic regurgitation of rheumatic etiology

N-Acetylglucosamine (GlcNAc) is the major immunoepitope of group A streptococcal cell wall carbohydrates. Antistreptococcal antibodies cross-reactive with anti-GlcNAc and laminin are present in sera of patients with rheumatic fever. The cross-reactivity of these antibodies with human heart valvular endothelium and the underlying basement membrane has been suggested to be a possible cause of immune-mediated valve lesion. Mannose-binding lectin (MBL) encoded by the MBL2 gene, a soluble pathogen recognition receptor, has high affinity for GlcNAc. We postulated that mutations in exon 1 of the MBL2 gene associated with a deficient serum level of MBL may contribute to chronic severe aortic regurgitation (AR) of rheumatic etiology. We studied 90 patients with severe chronic AR of rheumatic etiology and 281 healthy controls (HC) for the variants of the MBL2 gene at codons 52, 54, and 57 by using a PCR-restriction fragment length polymorphism-based method. We observed a significant difference in the prevalence of defective MBL2 alleles between patients with chronic severe AR and HC. Sixteen percent of patients with chronic severe AR were homozygotes or compound heterozygotes for defective MBL alleles in contrast to 5% for HC (P = 0.0022; odds ratio, 3.5 [ 95% confidence interval, 1.6 to 7.7]). No association was detected with the variant of the MASP2 gene. Our study suggests that MBL deficiency may contribute to the development of chronic severe AR of rheumatic etiology.

Effect of Disease Severity on the Performance of Cirrus Spectral-Domain OCT for Glaucoma Diagnosis

PURPOSE. To evaluate the effect of disease severity on the diagnostic accuracy of the Cirrus Optical Coherence Tomograph (Cirrus HD-OCT; Carl Zeiss Meditec, Inc., Dublin, CA) for glaucoma detection. METHODS. One hundred thirty-five glaucomatous eyes of 99 patients and 79 normal eyes of 47 control subjects were recruited from the longitudinal Diagnostic Innovations in Glaucoma Study (DIGS). The severity of the disease was graded based on the visual field index (VFI) from standard automated perimetry. Imaging of the retinal nerve fiber layer (RNFL) was obtained using the optic disc cube protocol available on the Cirrus HD-OCT. Pooled receiver operating characteristic (ROC) curves were initially obtained for each parameter of the Cirrus HD-OCT. The effect of disease severity on diagnostic performance was evaluated by fitting an ROC regression model, with VFI used as a covariate, and calculating the area under the ROC curve (AUCs) for different levels of disease severity. RESULTS. The largest pooled AUCs were for average thickness (0.892), inferior quadrant thickness (0.881), and superior quadrant thickness (0.874). Disease severity had a significant influence on the detection of glaucoma. For the average RNFL thickness parameter, AUCs were 0.962, 0.932, 0.886, and 0.822 for VFIs of 70%, 80%, 90%, and 100%, respectively. CONCLUSIONS. Disease severity had a significant effect on the diagnostic performance of the Cirrus HD-OCT and thus should be considered when interpreting results from this device and when considering the potential applications of this instrument for diagnosing glaucoma in the various clinical settings. (Invest Ophthalmol Vis Sci. 2010;51:4104-4109) DOI:10.1167/iovs.094716

Fibrinolytic activity is associated with presence of cystic medial degeneration in aneurysms of the ascending aorta

Fibrinolytic activity is associated with presence of cystic medial degeneration in aneurysms of the ascending aorta Aims: Thoracic ascending aortic aneurysms (TAA) are characterized by elastic fibre breakdown and cystic medial degeneration within the aortic media, associated with progressive smooth muscle cell (SMC) rarefaction. The transforming growth factor (TGF)-beta/Smad2 signalling pathway is involved in this process. Because the pericellular fibrinolytic system activation is able to degrade adhesive proteins, activate matrix metalloproteinase (MMP), induce SMC disappearance and increase the bioavailability of TGF-beta, the aim was to investigate the plasminergic system in TAA. Methods and results: Ascending aortas [21 controls and 19 TAAs (of three different aetiologies)] were analysed. Immunohistochemistry showed accumulation of t-PA, u-PA and plasmin in TAAs, associated with residual SMCs. Overexpression of t-PA and u-PA was confirmed by reverse transcription-polymerase chain reaction (RT-PCR), immunoblotting and zymography on TAA extracts and culture medium conditioned by TAA. Plasminogen was present on the SMC surface and inside cytoplasmic vesicles, but plasminogen mRNA was undetectable in the TAA medial layer. Plasmin-antiplasmin complexes were detected in TAA-conditioned medium and activation of the fibrinolytic system was associated with increased fibronectin turnover. Fibronectin-related material was detected immunohistochamically in dense clumps around SMCs and colocalized with latent TGF-beta binding protein-1. Conclusions: The fibrinolytic pathway could play a critical role in TAA progression, via direct or indirect impact on ECM and consecutive modulation of TGF-beta bioavailability.

Feline injection-site sarcoma

The feline injection-site sarcoma (FIS) is a challenge for the veterinarian and the affected cat`s owner. The injectable applications (vaccines, medications) seems to be the reason for that neoplasia, more specifically, the inflammation caused by injury of given drugs or antigens to the health tissue. Generally the FIS presents a more aggressive behavior when compared to sarcoma not associated to application. The most effective treatment his not been established yet, but it is believed that a multimodality of therapies, surgery, radiotherapy, and chemotherapy would be the most indicated option. The knowledge of the illness in all of its aspects will Supply to professionals colleges subsidies in relation to the best way to approach its diagnosis and treatment.

Inquiry of antibodies anti-Neospora caninum in dairy cattle, dogs and rural workers of the south-west region of Mato Grosso State

Considering the importance of neosporosis in the animal health and production, the frequency of antibodies to Neospora caninum was evaluated in dairy cattle of the Southwestern region of Mato Grosso State, Brazil, in addition to serum samples obtained from dogs and humans living in the farms. A total of 1036 serum samples were analyzed, from which 932 were from dairy bovine females, 37 from dogs and 67 from humans, from 24 farms and examined by the indirect fluorescent antibody test (IFAT). Reactive human scrum samples were retested by Western-blotting to confirm the results. Antibodies to N. caninum were found in 499 cattle sera (53.5 %), with at least one positive in each farm, 25 dog sera (67.6 %) and seven human sera (10.5 %). There was no significant difference in the number of positive cattle sera according to age group. The results indicate a wide dissemination of N caninum in the studied region.

Differential patterns of receptor activator of nuclear factor kappa B ligand/osteoprotegerin expression in human periapical granulomas: Possible association with progressive or stable nature of the lesions

Receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) are expressed in apical periodontitis, suggesting a role for these molecules during lesion development. However, the profiles of RANKL/OPG expression in periapical lesions remain unknown. In this study we investigated the patterns of RANKL and OPG mRNA expression by real-time polymerase chain reaction in human periapical granulomas (N = 44) and compared them with sites presenting characteristic bone resorbing activity: healthy (n = 14) and orthodontically stretched and compressed periodontal ligament (n = 26), healthy gingiva (n = 24), chronic gingivitis (n = 32), and chronic periodontitis (n = 34) samples. Both RANKL and OPG mRNA expression was higher in periapical granulomas when compared with healthy periodontal ligament. Distinct patterns of RANKL and OPG expression ratio were found in the granulomas and in different physiologic and pathologic conditions, with characteristic bone resorption activity potentially being indicative of the stable or progressive nature of the lesions. Lesions with radiographic image smaller than 5 mm showed higher RANKL/OPG expression than images greater than 5 mm. Periapical granulomas presented heterogeneous patterns of RANKL and OPG expression, ranging from samples with RANKL/OPG ratio similar to that seen in sites with minimal or absent bone resorption to samples with RANKL/OPG expression pattern comparable with active bone resorption sites.

Moderniza????o tecnol??gica

O Banco do Nordeste chegara ?? metade da presente d??cada eivado dos v??cios da grande maioria das empresas ent??o contempor??neas: uma estrutura cl??ssica de gest??o, organizada de modo fortemente centralizado, uma ??nfase negocial em administra????o e redu????o de custos e um modelo tecnol??gico voltado para controle, administra????o e contabiliza????o. Esse ??ltimo aspecto refletia uma s??rie de desvios. Nesse contexto, o Projeto Moderniza????o Tecnol??gica foi concebido para implementa????o de a????es e solu????es de tecnologia da informa????o para dar suporte aos neg??cios e gest??o da informa????o do Banco, provendo-o de moderna infra-estrutura, de nova plataforma de softwares b??sicos e aplicativos em ambiente cliente-servidor. A partir de ent??o, incorporou-se ?? cultura da empresa uma forte e sempre constante tend??ncia ?? inova????o, consolidado um novo perfil que destaca o Banco do Nordeste, inexoravelmente, entre as empresas l??deres no uso da tecnologia da informa????o, tanto no setor p??blico quanto no setor privado. O esfor??o empreendido implicou direta ou indiretamente melhorias de todos os processos de trabalho da empresa, resultando em benef??cios concretos no atendimento aos clientes

WAML World Congress: Direito Médico Mundial em Portugal

Eis que existem boas notícias científicas em Portugal. O WAML World Congress é o maior e mais prestigiado congresso mundial de direito médico. E, desta vez, vai ter lugar em Portugal, mais em concreto na Cidade capital do Mondego, Coimbra, entre os próximos dias 2 e 7 de Agosto de 2015. O seu Presidente ou Chairman, será o especialista português em direito médico, professor da Faculdade de Direito da Universidade de Coimbra, Doutor André Gonçalo Dias Pereira. Especialista que já esteve a fazer um excelente Seminário em Barcelos, Escola Superior de Gestão do Instituto Politécnico do Cávado e do Ave, no dia 26 de Maio de 2014, com o título de “O Direito da Concorrência: análise de um caso real”. Tema que relacionou com a poderosa indústria farmacêutica, entre outros aspectos conexos. § Behold, there are good scientific news in Portugal. The WAML World Congress is the largest and most prestigious world congress of medical law. And this time, will take place in Portugal, more specifically in the Mondego capital city, Coimbra, between 2nd and August 7, 2015. The President or Chairman, will be the Portuguese expert in medical law professor Faculty of Law, University of Coimbra, Doctor André Gonçalo Dias Pereira. Specialist who has been doing an excellent Seminar in Barcelos, School of Management Cávado Polytechnic Institute and Ave, on May 26, 2014, with the title "The Competition Law: analysis of a real case." Theme related to the powerful pharmaceutical industry, and other related aspects.

Sazonalidade e potencial energético da biomassa residual agrícola na região dos Campos Gerais do Paraná

RESUMO Teve-se por objetivo, neste trabalho, avaliar as disponibilidades sazonal e espacial da biomassa residual da colheita das principais culturas de grãos, na região dos Campos Gerais, PR, estimar o potencial teórico de produção de energia elétrica a partir desses resíduos, bem como identificar os municípios da região com maior potencial para instalação de termelétricas movidas a biomassa. Dados de produtividade de milho, soja, trigo, cevada, aveia branca e aveia preta foram obtidos, na Secretaria de Agricultura do Paraná, para as safras 2008/2009 e 2009/2010 e, a partir do índice e do calendário de colheitas, estimou-se a produção e sazonalidade da biomassa residual das culturas analisadas. Foram coletadas amostras de biomassa destas espécies e determinou-se o poder calorífico superior (PCS) e o potencial teórico de produção de energia elétrica a partir dos resíduos quantificados. Os Campos Gerais do Paraná produziram 3.862.130 (2008/2009) e 4.107.932 Mg ha−1 (2009/2010) de biomassa residual total com o cultivo das seis culturas. A disponibilidade de biomassa concentra-se em março e abril (aproximadamente 60%), sendo inexpressiva em dezembro, janeiro e entre junho e agosto. A biomassa residual da colheita de grãos apresentou PCS comparável ao de espécies florestais e potencial total de produção de energia de 3.932 (2008/2009) e 4.185 GWh ano−1 (2009/2010). Dos municípios analisados, Tibagi e Castro são os mais promissores para a instalação de termelétricas movidas a biomassa, por apresentarem maior produção e melhor distribuição sazonal dos resíduos.

Informação ou dramatização do risco: os media e os novos riscos

Também transversal a todos os casos analisados é o importante papel desempenhado pelos meios de comunicação social. Nas sociedades contemporâneas, os conflitos sociais apenas adquirem existência na esfera pública quando recebem cobertura mediática. No caso particular das questões de risco, tal é especialmente verdade. Os mass media, por via do chamado efeito do Agenda Setting, conferem existência a problemas que de outra forma estariam ausentes da esfera pública. Ao divulgarem um caso de risco, pressionam o poder político para tomar medidas ou justificar as opções tomadas, assim como a comunidade científica para sustentar o aconselhamento prestado.

Seismic resistance of earth construction in Portugal

This paper presents an assessment of the potential seismic performance of new earth construction in Portugal. Results of a parametric study on a properly designed rammed earth construction, considering several strengthening solutions, are presented and discussed. It is concluded that single storey houses can have acceptable seismic performance even in high seismicity areas, provided that the structure is adequately strengthened by reinforced concrete columns and beams. Improvement of the characteristics of the structural material (earth) may be necessary to reduce damage.