985 resultados para 321-U1338C
Resumo:
Pseudomonas aeruginosa has a pair of distinct ornithine carbamoyltransferases. The anabolic ornithine carbamoyltransferase encoded by the argF gene catalyzes the formation of citrulline from ornithine and carbamoylphosphate. The catabolic ornithine carbamoyltransferase encoded by the arcB gene promotes the reverse reaction in vivo; although this enzyme can be assayed in vitro for citrulline synthesis, its unidirectionality in vivo is determined by its high concentration at half maximum velocity for carbamoylphosphate ([S]0.5) and high cooperativity toward this substrate. We have isolated mutant forms of catabolic ornithine carbamoyltransferase catalyzing the anabolic reaction in vivo. The corresponding arcB mutant alleles on a multicopy plasmid specifically suppressed an argF mutation of P. aeruginosa. Two new mutant enzymes were obtained. When methionine 321 was replaced by isoleucine, the mutant enzyme showed loss of homotropic cooperativity at physiological carbamoylphosphate concentrations. Substitution of glutamate 105 by lysine resulted in a partial loss of the sigmoidal response to increasing carbamoylphosphate concentrations. However, both mutant enzymes were still sensitive to the allosteric activator AMP and to the inhibitor spermidine. These results indicate that at least two residues of catabolic ornithine carbamoyltransferase are critically involved in positive carbamoylphosphate cooperativity: glutamate 105 (previously known to be important) and methionine 321. Mutational changes in either amino acid will affect the geometry of helix H2, which contains several residues required for carbamoylphosphate binding.
Resumo:
En aquesta monografia Hubert Bessat ens presenta la primera part del seu estudi sobre el parlar de les Contamines-Montjoie. El poble de les Contamines-Montjoie (departament de l"Alta Savoia) està situat al sud del Montblanc, en una vall oberta al nord, en el territori francoprovençal de França a tocar de la Vall d"Aosta, aquesta ja a Itàlia.
Resumo:
Collection : Théâtre contemporain illustré ; 321e et 322e livraisons
Resumo:
Tesis (Maestría en Ciencias de la Ingeniería Mecánica con Especialidad en Materiales) UANL, 2013.
Resumo:
Incluye cuatro tipos de índices que facilitan la búsqueda de los artículos publicados en los números 321 a 323 y en el número extraordinario del año 2000. En el primer índice, los artículos se agrupan por notaciones de la Clasificación Decimal Universal (CDU) y en los siguientes por orden alfabético de autores, títulos y materias.
Resumo:
Jornal elaborado pela Assessoria de Comunicação e Imprensa da Reitoria da UNESP