995 resultados para 220
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Este trabalho se propõe a analisar a posse dos bens públicos, sob a perspectiva do neoconstitucionalismo, com destaque para o princípio da função social da propriedade. A tese deste estudo se pautou na afirmativa de que a partir do surgimento da concessão de uso especial para fins de moradia, instituída pela Medida Provisória 2.220 para regulamentar o artigo 183, § 1º da Constituição Federal de 1988, a função social da propriedade, antes sobrelevada nos litígios envolvendo a posse dos bens públicos, passou a ser discutida no âmbito dos tribunais. Para a comprovação da referida tese, analisou-se a jurisprudência do Superior Tribunal de Justiça, dos Tribunais Regionais Federais das cinco regiões do país e dos Tribunais de Justiça do Estado do Rio de Janeiro e do Rio Grande do Sul. Feita esta análise, foi possível comprovar a tese defendida. Com o intuito de garantir a máxima eficácia ao princípio da função social da propriedade, defendeu-se a não delimitação temporal imposta pela MP 2.220, tendo por base quatro argumentos de índole constitucional, sendo eles, a observância da força normativa da Constituição, a aplicação dos tratados internacionais de Direitos Humanos, o respeito ao princípio da igualdade e, por fim, o princípio da supremacia da Constituição.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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To find the regions having a major influence on the bioluminescence spectra of railroad worm luciferases, we constructed new chimeric luciferases switching the fragments from residues 1-219 and from 220-545 between Phrixotrix viviani (PxvGR; λmax = 548 nm) green light-emitting luciferase and Phrixothrix hirtus (PxhRE; λmax = 623 nm) red light-emitting luciferases. The emission spectrum (λmax = 571 nm) and KM for luciferin in the chimera PxRE220GR (1-219, PxhRE; 220-545, PxvGR) suggested that the region above residue 220 of PxvGR had a major effect on the active site. However, switching the sequence between the residues 226-344 from PxvGR luciferase into PxhRE (PxREGRRE) luciferase resulted in red light emission (λmax = 603 nm), indicating that the region 220-344 by itself does not determine the emission spectrum. Furthermore, the sequence before residue 220 of the green-emitting luciferase is incompatible for light emission with the sequence above residue 220 of PxhRE. These results suggest that the fragments before and after residue 220, which correspond to distinct subdomains, may fold differently in the green- and red-emitting luciferases, affecting the active site conformation.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Background: The number of Escherichia coli in the gut of Crohn's disease (CD) patients is higher than that of normal subjects, but the virulence potential of these bacteria is not fully known. Previous studies have shown that these E. coli are closely related to extraintestinal pathogenic categories (ExPEC), are able to invade epithelial cells, and usually do not produce exotoxins. We report here the detection, in a CD patient, of an E. coli which belongs to a classical enteropathogenic (EPEC) serotype and displays virulence markers of enteroinvasive (EIEC), enteroaggregative (EAEC) and enterohemorrhagic (EHEC) pathotypes. Methods: The E. coli strain was isolated, in 2009, by classical bacteriological procedures from a 56 year old woman who underwent ileo-terminal resection 1 year before, due to intestinal obstruction. The bacterial characterization was carried out by in vitro adhesion and invasion assays to cultured epithelial cells and macrophages and screening by PCR to identify virulence genetic markers of diarrheogenic E. coli (DEC) and to detect one of the gene combinations which define the phylogroups of the E. coli reference (EcoR) collection. The strain was also tested for the ability to produce biofilm and shiga cytotoxins and had its whole genome sequenced by Ion Torrent Sequencing Technology. Results: The studied strain, which was detected both in ileum biopsies and the stools of the patient, displayed the aggregative adherence (AA) phenotype to Hep-2 cells and an ability to enter Caco-2 cells 3x as high as that of EIEC reference strain and 89% of that of the prototype AIEC LF82 strain. Although it could invade cultured macrophages, the strain was unable to replicate inside these cells. PCR screening revealed the presence of eae, aggR and stx1. Tests with bacterial culture supernatants in Vero cells demonstrating cytotoxicity suggested the production of Stx1. In addition, the strain revealed to be a strong biofilm producer, belonged to the B2 EcoR phylogroup, to the O126:H27 serogroup and to the multilocus sequencing type (MLST) ST3057. The 2 later features were deduced from the whole genome sequence of the strain. Conclusions: The characterization of this E. coli isolate from a CD patient revealed a combination of virulence markers of distinct DEC pathotypes, namely eae and stx1 of EHEC, AA, aggR and biofilm formation of EAEC, and invasiveness of EIEC. These features along with its serotype and phylogroup identity seem to suggest a potential to be involved in CD, an observation which should be tested with additional studies.
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The Rock Hill Hardware Company was organized on June 4, 1893 by A.R. Smith and John Gelzer, A.A. Barron and his sons R.E. and W.L. bought Smith out in 1896 and by 1907 had acquired the whole firm. The Barron family owned and operated it until it closed in 1978. The collection consists of a 1906 ledger, financial records, a photograph, a seed license, World War II ration booklets, and newspaper clippings.