Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells

Osteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells.

Associação do hormônio tireoidiano ao estrógeno em células de rato ROS 17/2.8 (modelo experimental de células osteoblásticas)

Os mecanismos pelos quais o hiper e hipotireoidismo afetam o osso durante a menopausa são pouco conhecidos. O objetivo deste estudo foi verificar a ação do hormônio triiodotironina (T3), em diferentes concentrações associado ao estrógeno (E2) em concentração infra-fisiológica (condição de menopausa), sobre a proliferação de células de rato ROS 17/2.8, através de uma curva de crescimento para obtenção do tempo de dobramento (TD). As células ROS 17/2.8 são consideradas modelo de células osteoblásticas e foram cultivadas em meio HAM F-12, e submetidas ao tratamento hormonal com as combinações das concentrações fisiológica, infrafisiológica e suprafisiológica de T3 e infra-fisiológica de E2. Utilizamos como grupo controle aquele tratado com as concentrações fisiológicas dos dois hormônios. Para cada concentração hormonal foram realizadas 5 repetições, e as células contadas manualmente nos dias 1, 3, 5 e 7, após o início da adição hormonal. A análise estatística utilizada foi a técnica da análise de variância não paramétrica, complementada com teste de comparações múltiplas. Baseando-se nos resultados obtidos, pode-se concluir que o crescimento das células ROS 17/2.8 é afetado pelos tratamentos hormonais, em especial quando a concentração infrafisiológica de E2 está associada à concentração suprafisiológica de T3, aumentando o tempo de dobramento celular. Este efeito poderia tornar o metabolismo do osso mais lento, prejudicando a neoformação óssea em pacientes menopausadas e hipertireoidéas

Influência das células mioepiteliais neoplásicas benignas de adenoma pleomórfico na produção de MMPs -1, -2, - 8, -9 e -13 por células epiteliais malignas e sua possível relação com o índice de invasão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Treatment outcome of 42 replanted permanent incisors with a median follow-up of 2.8 years

To evaluate the treatment outcome of avulsed and replanted permanent incisors.

Iron(III)-Pivalate-Based Complexes with Tetranuclear {Fe-4(mu(3)-O)(2)}(8+) Cores and N-Donor Ligands: Formation of Cluster and Polymeric Architectures

Different synthetic routes have been used for the preparation of a new tetranuclear [Fe4O2(O2CCMe3)(8)(bpm)] cluster (1) and a one-dimensional coordination polymer [Fe4O2-(O2CCMe3)(8)(hmta)](n) (2) (bpm = 2,2'-bipyrimidine and hmta = hexamethylenetetramine). For cluster 1, two structural isomers, 1a and 1b center dot 3MeCN, have been found. X-ray crystallographic analysis showed that all complexes consist of a central {Fe-4(mu(3)-O)(2)}(8+) core. In 1a, metal ions in the core are additionally linked by six bridging pivalates as two other pivalates and a bpm ligand are chelated to Fe-III ions, whereas in cluster 1b, metal ions in the {Fe-4(mu(3)-O)(2)}(8+) core are linked by seven bridging pivalates and only one carboxylate as well as bpm are chelated to the iron centers. In coordination polymer 2, [Fe4O2(O2CCMe3)(8)] clusters are bridged by hmta ligands to form zigzag chains. Magnetic measurements have been carried out to characterize these complexes and revealed antiferromagnetic interactions between Fe-III ions with best-fit parameters of J(wb) = -72.2 (1a) and -88.7 cm(-1) (1b) for wing...body interactions.

Na 1 Nachlass Max Horkheimer, 700 - "Labor Under National Socialism" "Notes on a Study in News Commentators" Forschungsprojekte und Memoranden zur Umgestaltung Nachkriegs-Deutschlands, besonders zur Umerziehung (p. IX 170a - IX 172.2-8)

Neumann, Franz L.: "Labor Under National Socialism", 19.03.1942. Typoskript, 66 Blatt; Löwenthal, Leo: "Notes on a Study in News Commentators" 23.01.1943; "Notes on a Study on News Commentators". Typoskript, 34 Blatt; "Treatment of Selected New Topics in News and News Commentator Programs". Typoskript, 53 Blatt; Forschungsprojekte und Memoranden zur Umgestaltung Nachkriegs-Deutschlands, besonders zur Umerziehung, 1942-1949; 1. "Project To Survey Present German Educational Practices in the Field of Social Sciences as a Means for Democratization. Supplementary Statements" 28.02.1949; a) Teilstück, Typoskript, 1 Blatt; b)-f) Typoskripte, zum Teil mit eigenhändigen Korrekturen von Max Horkheimer, 21 Blatt; g) Eigenhändige Notizen von Max Horkheimer, 1 Blatt; h) Eigenhändige Notizen von Theodor W. Adorno, 1 Blatt; 2. Marcuse, Herbert: 2 Briefe mit Unterschrift an Max Horkheimer und Beil, ohne Ort, 1949; 1 Brief mit Unterschrift von Max Horkheimer, Pacific Palisades, 25.02.1949; 3. "German Project" a) Typoskript mit handschriftlichen Korrekturen, 12 Blatt; b) Typoskript mit eigenhändigen Korrekturen von Max Horkheimer, 12 Blatt; c) Typoskript mit eigenhändigen Korrekturen von Max Horkheimer, 5 Blatt; d) Eigenhändige Notizen von Max Horkheimer, 1 Blatt; 4. Emhardt, K.H.: 1 Briefabschrift an Max Horkheimer, München, 20.06.1948, 1 Blatt; 5. "Untersuchunge über die Durchführung und das Ergebnis der politischen Säuberung an den Hochschulen der Westzone" a) Typoskript, 4 Blatt; b) Typoskript mit handschriftlichen Korrekturen, 2 Blatt; 6. Über Antisemitismus und politische Fragen im Nachkriegsdeutschland. Auszug aus einem Brief von "F.L.", 1949, Typoskirpt, 9 Blatt; 7. "Liste of Signers of the 1933 manifesto". 1 Blatt; 8. Marcuse, Herbert: Über Probleme der Demokratisierung und des Chauvinismus im Nachkriegsdeutschland. Teilstück eines Typoskripts, 4 Blatt, mit einem eigenhändigen Brief mit Unterschrift an Leo Löwenthal, ohne Ort, 25.11.1948, 1 Blatt;