(Table 1) Porewater analysis with respect to gypsum from different ODP holes

The stability of gypsum in marine sediments has been investigated through the calculation of its saturation index at the sediment in situ temperature and pressure, using the entire ODP/IODP porewater composition database (14416 samples recovered from sediments collected during 95 ODP and IODP Legs). Saturation is reached in sediment porewaters of 26 boreholes drilled at 23 different sites, during 12 ODP/IODP Legs. As ocean bottom seawater is largely undersaturated with respect to gypsum, the porewater Ca content or its SO4 concentration, or both, must increase in order to reach equilibrium. At several sites equilibrium is reached either through the presence of evaporitic gypsum layers found in the sedimentary sequence, and/or through a salinity increase due to the presence of evaporitic brines with high concentrations of Ca and SO4. Saturation can also be reached in porewaters of seawater-like salinity (~ 35 per mil), provided sulfate reduction is limited. In this case, saturation is due to the alteration of volcanogenic material which releases large amounts of Ca to the porewaters, where the Ca concentration can reach 55 times its seawater value as for example at ODP Leg 134 site 833. At a few sites, saturation is reached in hydrothermal environments, or as a consequence of the alteration of the basaltic basement. In addition to the well known influence of brines on the formation of gypsum, these results indicate that the alteration of sediments rich in volcanogenic material is a major process leading to gypsum saturation in marine sediment porewaters. Therefore, the presence of gypsum in ancient and recent marine sediments should not be systematically interpreted as due to hypersaline waters, especially if volcanogenic material is present.

Intermittent Fugu parathyroid hormone 1 (1–34) is an anabolic bone agent in young male rats and osteopenic ovariectomized rats

Human parathyroid hormone (hPTH) is currently the only treatment for osteoporosis that forms new bone. Previously we described a fish equivalent, Fugu parathyroid hormone 1 (fPth1) which has hPTH-like biological activity in vitro despite fPth1(1–34) sharing only 53% identity with hPTH(1–34). Here we demonstrate the in vivo actions of fPth1(1–34) on bone. In study 1, young male rats were injected intermittently for 30 days with fPth1 [30 μg–1000 μg/kg body weight (b.w.), (30fPth1–1000fPth1)] or hPTH [30 μg–100 μg/kg b.w. (30hPTH–100hPTH)]. In proximal tibiae at low doses, the fPth1 was positively correlated with trabecular bone volume/total volume (TbBV/TV) while hPTH increased TbBV/TV, trabecular thickness (TbTh) and trabecular number (TbN). 500fPth1 and 1000fPth1 increased TbBV/TV, TbTh, TbN, mineral apposition rate (MAR) and bone formation rate/bone surface (BFR/BS) with a concomitant decrease in osteoclast surface and number. In study 2 ovariectomized (OVX), osteopenic rats and sham operated (SHAM) rats were injected intermittently with 500 μg/kg b.w. of fPth1 (500fPth1) for 11 weeks. 500fPth1 treatment resulted in increased TbBV/TV (151%) and TbTh (96%) in the proximal tibiae due to increased bone formation as assessed by BFR/BS (490%) and MAR (131%). The effect was restoration of TbBV/TV to SHAM levels without any effect on bone resorption. 500fPth1 also increased TbBV/TV and TbTh in the vertebrae (L6) and cortical thickness in the mid-femora increasing bone strength at these sites. fPth1 was similarly effective in SHAM rats. Notwithstanding the low amino acid sequence homology with hPTH (1–34), we have clearly established the efficacy of fPth1 (1–34) as an anabolic bone agent.

The role of His-134, -147, and -150 residues in subunit assembly, cofactor binding, and catalysis of sheep liver cytosolic serine hydroxymethyltransferase

In an attempt to unravel the role of conserved histidine residues in the structure-function of sheep liver cytosolic serine hydroxymethyltransferase (SHMT), three site-specific mutants (H134N, H147N, and H150N) were constructed and expressed, H134N and H147N SHMTs had K-m values for L-serine, L-allo-threonine and beta-phenylserine similar to that of wild type enzyme, although the k(cat) values were markedly decreased, H134N SHMT was obtained in a dimeric form with only 6% of bound pyridoxal 5'-phosphate (PLP) compared with the wild type enzyme, Increasing concentrations of PLP (up to 500 mu M) enhanced the enzyme activity without changing its oligomeric structure, indicating that His-134 may be involved in dimer-dimer interactions, H147N SHMT was obtained in a tetrameric form but with very little PLP (3%) bound to it, suggesting that this residue was probably involved in cofactor binding, Unlike the wild type enzyme, the cofactor could be easily removed by dialysis from H147N SHMT, and the apoenzyme thus formed was present predominantly in the dimeric form, indicating that PLP binding is at the dimer-dimer interface, H150N SHMT was obtained in a tetrameric form with bound PLP, However, the mutant had very little enzyme activity (<2%). The k(cat)/K-m values for L-serine, L-allo-threonine and beta-phenylserine were 80-, 56-, and SS-fold less compared with wild type enzyme, Unlike the wild type enzyme, it failed to form the characteristic quinonoid intermediate and was unable to carry out the exchange of 2-S proton from glycine in the presence of H-4-folate. However, it could form an external aldimine with serine and glycine, The wild type and the mutant enzyme had similar K-d values for serine and glycine, These results suggest that His-150 may be the base that abstracts the alpha-proton of the substrate, leading to formation of the quinonoid intermediate in the reaction catalyzed by SHMT.

Sapientia, 1979, Vol. XXXIV, nº 133-134 (número completo)

Contenido: El orden humano / Octavio N. Derisi – Los primeros principios / Gustavo E. Ponferrada – La filosofía analítica actual y su terapia mediante la filosofía cristiana y tomista / Teófilo Urdánoz – Verdad y relativismo según el pensamiento de Tomás de Aquino / William R. Darós – El mundo espiritual de la persona / Octavio N. Derisi – Ordo et mysterium / Emilio Komar – La filosofía cristiana de la libertad / Abelardo Lobato – El significado de la encíclica “Aeterni Patris” de León XIII, a los cien años de su publicación / Octavio N. Derisi – Notas y comentarios -- Bibliografía

Diário da Constituinte [gravação de vídeo] : [programa n. 134]

Após muita discussão e duas reuniões das lideranças partidárias, foi aprovado na Comissão de Sistematização o projeto da nova Constituição. Fernando Henrique Cardoso (PMDB-SP) diz que houve acordo entre as lideranças para a votação do projeto formulado por Bernardo Cabral (PMDB-AM). Sandra Cavalcanti (PFL-RJ) fez críticas ao trabalho de Bernardo Cabral, mas depois desmentiu o que foi divulgado pela imprensa. Afonso Arinos (PFL-RJ) lê nota citando o desmentido e dando integral apoio ao Relator Bernardo Cabral. Gasthone Righi (PTB-SP) quer que a Comissão de Sistematização continue apreciando as emendas dos constituintes. Cronograma dos trabalhos na Assembleia Nacional Constituinte (ANC) : discussão e apresentação de emendas no dia 14 de julho; fim do prazo para apresentar emendas no dia 14 de agosto; encerramento da primeira fase de discussão em Plenário no dia 24 de Agosto ; a Comissão de Sistematização volta a discutir o projeto no dia 25 de agosto; encerramento do prazo para votação do substitutivo na Comissão de Sistematização no dia 20 de Setembro; início da votação em 1º turno no Plenário no dia 23 de setembro. Se todos os prazos forem cumpridos, a nova Constituição será promulgada ainda em 1987 e entrará em vigor no dia 1º de janeiro de 1988. Percival Muniz (PMDB-MT) incentiva a população a participar dos debates da Assembleia Constituinte.

~(134)Ba能级结构的在束γ谱学研究

利用在束γ谱学技术 ,通过 12 8Te(10 B ,1p3n) 13 4 Ba反应研究了 13 4 Ba的中、高自旋激发态 .实验中采用 9套BGO(AC)HPGe探测器进行了γ γ t符合测量 .基于γ γ符合关系、γ射线的相对强度和各向异性度的测量结果建立了 13 4 Ba的能级纲图 .比较N =78的同中子素链的负宇称能级结构的系统性 ,发现激发能位于 4 14 2keV ,Jπ 值为 11-的能级很可能是与 13 6Ce具有相似内禀结构特征的相似态

L'honneur de la maison : drame en 5 actes. [suivi de] Le laquais d'Arthur : comédie en 1 acte en prose. Livr. 134 / par MM. Léon Battu et Maurice Desvignes (M.-F.-J.-B. Delavigne) ; par M. René Lordereau

Collection : Théâtre contemporain illustré ; 133e et 134e livraisons

Livre des Morts de Djéhoutyirydès. Egyptien 134

La grande scène au trait est celle du jugement du chapitre 125 du Livre des Morts. On y voit le défunt devant la déesse Maât, la balance où le coeur est pesé sous la surveillance d'Anubis, Horus et Thot en sa qualité de scribe. La grande dévoreuse est présente, devant Osiris, lequel est assis sur son trône.Texte :Col. 1, 1-14 et vignette : chapitre 137 A avec titre rubriqué : "Formule des quatres torches". La vignette montre le défunt assis entouré de quatre lampes.Col. 1, 15-25 et vignette : chapitre 138 avec titre rubriqué : "Formule pour entrer en Abydos". Le défunt est debout devant deux enseignes.Col. 2 et vignette : chapitre 141 avec titres rubriqués : "Formule pour connaître le nom des dieux du ciel méridional; Livre pour transfigurer les esprits, pour les dieux du ciel méridional et les dieux du ciel septentrional, et les dieux qui sont dans la nécropole septentrionale, et les dieux qui conduisent dans la Douat, que l'on fait pour un homme, pour son père ou pour sa mère lors de la fête ; pour le glorifier dans le coeur de Rê et dans le coeur des dieux, de sorte qu'il soit avec eux. La vignette montre le défunt en adoration derrière Horus qui présente le signe de la vie à Osiris.