Características físico-químicas e citológicas do líquido sinovial da bainha tendínea digital de eqüinos

Foram estudadas as características físico-químicas e citológicas do líquido sinovial da bainha tendínea digital de nove eqüinos hígidos. Verificou-se que o líquido é viscoso, amarelo claro, límpido, livre de partículas e que não coagula à temperatura ambiente. Sua concentração média de ácido hialurônico foi 60,20mg/dl, a taxa de glicose, similar à plasmática e sua concentração protéica não ultrapassou 1,74g/dl, com relação média albumina:globulina de 0,94. O número médio de células nucleadas foi de 313 células/µl, com predominância de grandes células mononucleares e linfócitos. Houve correlação significativa (r = - 0,649, P<0,01) entre o aumento da concentração de ácido hialurônico e a diminuição percentual de linfócitos. As mensurações das características pesquisadas no líquido sinovial da bainha tendínea digital de eqüinos são de execução simples e passíveis de implantação na rotina de atendimentos clínico-cirúrgicos.

Efeito do tratamento com extrato de pituitaria equina (EPE) e hCG no índice de recuperação de oócitos e maturação folicular em equinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Determinação da seqüência de cDNA da enzima hialuronidase do veneno de Polybia paulista (Hymenoptera : vespidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise da cicatrização na pele de coelhos após tratamentos de feridas com biomateriais associados à fração de proteína do látex natural da seringueira (Hevea brasiliensis)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência da ação da hCG sobre a expressão gênica de epirregulina, ampirregulina e hialurona sintetase-2 em folículos ovarianos de éguas estimuladas ou não com extrato de pituitária equina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo do remodelamento da lâmina própria da laringe relacionado ao processo de senilidade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência de diferentes tratamentos físico-químicos na adesão e viabilidade de fibroblastos de ligamento periodontal à superfície radicular simulando dentes avulsionados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A voz do idoso: características clínicas, endoscópicas, vocais e morfológicas

Pós-graduação em Bases Gerais da Cirurgia - FMB

A voz do idoso: características clínicas, endoscópicas, vocais e morfológicas

Pós-graduação em Bases Gerais da Cirurgia - FMB

Viscosuplementação

Com o envelhecimento da população mundial, aumenta a cada dia a prevalência das doenças relacionadas à idade, com destaque para a osteoartrite, forma mais comum de doença articular e que, além da alta prevalência, relaciona-se a altos custos médicos e sociais. Dentre as modalidades de tratamento, a viscosuplementação, injeção intra-articular de derivados do ácido hialurônico, vem ganhando cada vez mais destaque. Trata-se de um polissacarídeo de alto peso molecular que possui, além das funções mecânicas de distribuição de peso e lubrificação, propriedades anti-inflamatórias e ação físico-química sobre diversos aspectos da articulação, sendo que tais efeitos são diretamente proporcionais ao peso molecular, concentração e presença de ligações cruzadas da droga utilizada. A viscosuplementação é procedimento simples e pode ser realizada ambulatorialmente. Traz benefício para dor e função, e também altera favoravelmente o curso da doença, melhorando quantitativamente e qualitativamente a cartilagem articular. Apresenta bom perfil de segurança e favorável relação custo-efetividade, sendo indicada tanto para osteoartrite quanto após um procedimento de artroscopia.

Engraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice

PURPOSE: To evaluate the implant of human adipose derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected in the subcutaneous of athymic mice. METHODS: Control implants -HA plus culture media was injected in the subcutaneous of the left sub scapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With eight weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxilin-eosin staining and immunufluorescence using anti human vimentin and anti von Willebrand factor antibodies. RESULTS: Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxilin-eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants - A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxilin- eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. CONCLUSION: The delivery of human adipose derived stem cells in preparations of hyaluronic acid assured cells engraftment at the site of injection.

Comparative study between the effects of hyaluronic acid and acid galactan purified from eggs of the mollusk Pomacea sp in wound healing

To compare the effect of hyaluronic acid (HA) and of AG on the healing of intestine wounds. Methods: The semi-purified extract of the eggs of the mollusc was obtained by fractionation with ammonium sulfate and purification for ion-exchange chromatography. The obtained galactans were eluted in water (neutral galactan) and in 0.1 and 0.2M NaCl (acidic galactans). The in vivo study was performed with 45 “Wistar” rats, separated in three groups (n=15). Solutions containing HA 1%, GA 1% or saline solution 0,9%, was placed topically on the sutures of wounds in the small intestine of the rats. After 05, 10 and 21 days the animals were sacrificed and biopsy of the healing tissue was done. Results: The hystologic grading was more significant for HA and AG groups when compared to the group C. AG stimulated the appearance of macrophages, giant cells and increase in the concentration of collagen in the area of the wound when compared to HA. Conclusion: The topical use of GA in intestinal wounds promoted the anticipation of events that are important in the wound healing

Hyaluronic acid as neuropathic ulcer treatment: a case report

The prevalence of diabetic polyneuropathy in Spain is 22% increasing with age, standing at less than 5% in patients between 15 and 19 years and reaching 29.8% in those aged 70 to 74 years age. Infection is an important complication in Diabetic Foot, frequently associated with minor amputation and even lower extremity amputation. The study presents a clinical case of a 68-year-old man who consulted for a diabetic foot ulcer in the metatarseal area of the right foot, diagnosed two years ago and without healing success. An exploration protocol of the diabetic food was made. Afterwards, a surgical debridement was done and a cure procedure with pure hyaluronic acid, a bandage and foot unloading was followed. After 69 days of treatment, a complete ulcer healing was achieved. After the injure healing, a biomechanical exploration was made and a plantar support was produced to avoid the reappearance of the injury because of local hyperpressure.

Characterization of the glial scar tissue in a murine model of spinal cord compression, with focus on hyaluronan

Spinal cord injury (SCI) is a devastating neurological disorder that affects thousands of people each year. Although in recent decades significant progress has been made in relation to understanding the molecular and cellular events underlying the nervous damage, spinal cord injury is still a highly disabling condition for which there is no curative therapy. People affected by spinal cord injuries manifested dysfunction or loss, temporary or permanent, of motor, sensory and / or autonomic functions depending on the spinal lesion damaged. Currently, the incidence rate of this type of injury is approximately 15-40 cases per million people worldwide. At the origin of these lesions are: road accidents, falls, interpersonal violence and the practice of sports. In this work we placed the hypothesis that HA is one of the component of the scar tissue formed after a compressive SCI, that it is likely synthetised by the perilesional glial cells and that it might support the permeation of the glial scar during the late phase of SCI. Nowadays, much focus is drawn on the recovery of CNS function, made impossible after SCI due to the high content of sulfated proteoglycans in the extracellular matrix. Counterbalancing the ratio between these proteoglycans and hyaluronic acid could be one of the experimental therapy to re-permeate the glial scar tissue formed after SCI, making possible axonal regrowth and functional recovery. Therefore, we established a model of spinal cord compression in mice and studied the glial scar tissue, particularly through the characterization of the expression of enzymes related to the metabolism of HA and the subsequent concentration thereof at different distances of the lesion epicenter. Our results show that the lesion induced in mice shows results similar to those produced in human lesions, in terms of histologic similarities and behavioral results. but these animals demonstrate an impressive spontaneous reorganization mechanism of the spinal cord tissue that occurs after injury and allows for partial recovery of the functions of the CNS. As regards the study of the glial scar, changes were recorded at the level of mRNA expression of enzymes metabolizing HA i.e., after injury there was a decreased expression of HA synthases 1-2 (HAS 1-2) and an increase of the expression HAS3 synthase mRNA, as well as the enzymes responsible for the HA catabolism, HYAL 1-2. But the amount of HA measured through the ELISA test was found unchanged after injury, it is not possible to explain this fact only with the change of expression of enzymes. At two weeks and in response to SCI, we found synthesized HA by reactive astrocytes and probably by others like microglial cells as it was advanced by the HA/GFAP+ and HA/IBA1+ cells co-location.

Tratamiento farmacológico invasivo articular en la artrosis

La degeneración de las articulaciones axiales y periféricas va unida al propio envejecimiento del ser humano. Es responsable del dolor e impotencia funcional, y su fisiopatología es compleja y no del todo conocida. El denominador común en la artrosis es la degeneración del cartílago articular, junto a otras alteraciones secundarias. Su abordaje y manejo del dolor supone todo un reto para las Unidades del Dolor. Históricamente, el tratamiento intervencionista se llevó a cabo mediante infiltraciones a ciegas de diferentes sustancias que alivian el dolor. En la actualidad, la fluoroscopia, y más recientemente los ultrasonidos, son los métodos de elección para la guía de nuestros procedimientos. En cuanto a los fármacos utilizados más comúnmente en nuestras técnicas invasivas articulares destacaremos el ácido hialurónico (AH) y el plasma rico en plaquetas (PRP). Los esteroides siguen siendo, en prácticamente todos los estudios, el estándar de oro o fármaco de referencia y sobre los que se comparan el resto de familias de fármacos. Una exploración exhaustiva del paciente, y un algoritmo de decisiones en cuanto a la decisión de realización de estas técnicas, se antoja necesario, adecuándose a cada lugar de trabajo.