Abrupt changes in high-latitude nutrient supply to the Atlantic during the last glacial cycle

The supply of nutrients to the low-latitude thermocline is largely controlled by intermediate-depth waters formed at the surface in the high southern latitudes. Silicic acid is an essential macronutrient for diatoms, which are responsible for a significant portion of marine carbon export production. Changes in ocean circulation, such as those observed during the last deglaciation, would influence the nutrient composition of the thermocline and, therefore, the relative abundance of diatoms in the low latitudes. Here we present the first record of the silicic acid content of the Atlantic over the last glacial cycle. Our results show that at intermediate depths of the South Atlantic, the silicic acid concentration was the same at the Last Glacial Maximum (LGM) as it is today, overprinted by high silicic acid pulses that coincided with abrupt changes in ocean and atmospheric circulation during Heinrich Stadials and the Younger Dryas. We suggest these pulses were caused by changes in intermediate water formation resulting from shifts in the subpolar hydrological cycle, with fundamental implications for the nutrient supply to the Atlantic.

Rickettsia bellii in ticks Amblyomma varium Koch, 1844, from birds in Peru

Amazonian birds were caught and examined for the presence of ectoparasites in the Allpahuayo Mishana National Reserve near Iquitos, Peru, from 13 to 16 August 2011. A total of 40 birds representing 16 species were examined. Two birds (5%) were infested with 2 larvae of Amblyomma varium Koch, 1844, and one nymph of A. calcaratum Neumann, 1899. The 2 larvae of A. varium were infected with Rickettsia bellii. This is the first report of R. bellii in A. varium and also the first record of this rickettsia in Peru. In addition, an immature A. calcaratum is reported from Peru for the first time. (c) 2012 Elsevier GmbH. All rights reserved.

Gregarious behavior of two species of Neotropical harvestmen (Arachnida: Opiliones: Gonyleptidae)

We present the first record and description of the gregarious behavior of the Neotropical harvestmen Serracutisoma proximum (Mello-Leitao 1922) and Serracutisoma spelaeum (Mello-Leitao 1933) (Opiliones: Gonyleptidae: Goniosomatinae) (DaSilva & Gnaspini 2010). We followed and described the pattern of these aggregations over a period of 17 months in a cave in southeastern Brazil. Individuals of the two species aggregated with both conspecifics and heterospecifics during the non-reproductive season (i.e., from October to March, the cool and dry season). Aggregations contained up to 81 individuals, usually with a female-biased adult sex ratio. Multispecific aggregations were usually composed mainly of representatives of one of the two species, suggesting that although these species also aggregate with heterospecifics, there is a preference for aggregating with conspecifics. This study provides novel information on the social behavior of harvestmen, specifically regarding the composition of multispecific aggregations.

Ticks (Acari: Ixodidae) associated with small terrestrial mammals in the state of Minas Gerais, southeastern Brazil

From June 2005 to November 2010, 43 small mammals encompassing 6 species of Didelphimorphia, 8 species of Rodentia, and 1 species of Lagomorpha were found parasitized by ticks in the state of Minas Gerais, southeastern Brazil. Nine tick species, in total 186 specimens, were identified as follows: Amblyomma cajennense (larvae and nymphs) on opossums and rodents; Amblyomma ovale (nymphs) on rodents; Amblyomma parvum (nymphs) on rodents; Amblyomma coelebs (nymphs) on opossums; Amblyomma dubitatum (nymph) on opossums; Ixodes amarali (females, nymphs, and larvae) on opossums and rodents; Ixodes loricatus (male, females, nymph) on opossums; Ixodes schulzei (female) on rodents; and Haemaphysalis leporispalustris (female) on rabbits. Most of the tick-host associations found in the present study have never been recorded in the literature; those include three new host records for I. amarali, four for A. cajennense, one for A. dubitatum, two for A. ovale, and one for A. coelebs. In addition, we provide the first record of A. coelebs in the state of Minas Gerais.

A description of the nymph of Anacroneuria ofaye Froehlich (Plecoptera: Perlidae) and a apparatus for rearing Neotropical stonefly species

The Neotropical genus Anacroneuria (Perlidae) is represented by more than 300 species. Among the Brazilian Anacroneuria, only the nymphs of two species have been described. In this paper, we described the nymph of A. ofaye Froehlich and an apparatus for rearing this genus. This is the first record of A. ofaye from Parana State, Brazil.

Non-functionant urinary bladder paraganglioma

Introduction: Paragangliomas are rare tumors origined on chromaffin-cells of neural crest and can be located from skull base to pelvis, on sympathetic or parasympathetic paraganglia. They account on less than 0,06% of all urinary bladder tumors, with only few hundreds of cases reported in literature since the first record by Zimmermmann in 1953. Case Report: A 63 year-old woman referring irritative urinary symptoms was submitted to an ultrassonography that disclosed an irregular-shaped nodulation on her bladder. CT confirmed the existence of a nodulation on bladder's anterior wall. Patient had normal levels of urinary catecholamins and Vanilmandelic acid. Tumor was excised and posterior immunohistochemical study revealed it was a paraganglioma. Nowadays, ten months after surgery, patient stills healthy and disease-free. Discussion: Paragangliomas can be classified as functionant or non-functionant, according to its production of cathecolamins, which can cause the same symptom complex of pheocromocytomas. About 10-15% of bladder paragangliomas are malignant, and potential metastasis are more common to lymph nodes, lungs and bones. 131-MIBG iodine cyntilography is the most sensitive method for diagnosis and surgery (transurethral resection or cystectomy) is the best choice for treatment.

The occurrence of defensive alkaloids in non-integumentary tissues of the Brazilian red-belly toad Melanophryniscus simplex (Bufonidae)

The red-belly toads (Melanophryniscus) of southern South America secrete defensive alkaloids from dermal granular glands. To date, all information on Melanophryniscus alkaloids has been obtained by extraction from either skins or whole organisms; however, in other amphibians, tetrodotoxins, samandarines, and bufadienolides have been detected in both skin and other organs, which raise the possibility that lipophilic alkaloids may occur in non-integumentary tissues in Melanophryniscus as well. To test this hypothesis, we studied the distribution of alkaloids in the skin, skeletal muscle, liver, and mature oocytes of the red-belly toad M. simplex from three localities in southern Brazil. Gas chromatography and mass spectrometry of skin extracts from 11 individuals of M. simplex resulted in the detection of 47 alkaloids (including isomers), 9 unclassified and 38 from 12 known structural classes. Each alkaloid that was present in the skin of an individual was also present in the same relative proportion in that individual's skeletal muscle, liver, and oocytes. The most abundant and widely distributed alkaloids were the pumiliotoxins 251D, 267C, and 323A, 5,8-disubstituted indolizidines 207A and 223D, 5,6,8-trisubstituted indolizidine 231B, 3,5-disubstituted pyrrolizidines cis-223B and cis- and trans-251K, and izidine 211C. We report the first record of piperidines in Melanophryniscus, bringing the total number of alkaloid classes detected in this genus to 16. Alkaloid composition differed significantly among the three study sites. The functional significance of defensive chemicals in non-integumentary tissues is unknown.

Resurrection of the genus Licornia for Scrupocellaria jolloisii (Bryozoa) and related species, with documentation of L. jolloisii as a non-indigenous species in the western Atlantic

Recent studies of the large cheilostome bryozoan genus Scrupocellaria have shown a greater degree of taxonomically informative morphological variation in zooids, opesia, and polymorphic structures than previously recognized. Only one subgenus has been named within the genus, Retiscrupocellaria d'Hondt, 1988, erected for Scrupocellaria jolloisii. In this work we further analyse S. jolloisii and its related species, resurrecting an earlier genus name, Licornia van Beneden, 1850 for Licornia jolloisii, and nine relatives, L. annectens, L. cervicornis, L. cyclostoma, L. diadema, L. ferox, L. gaspari, L. longispinosa, L. macropora, and L. prolata. Licornia jolloisii was originally described from the Red Sea, and most species of the genus occur in the Indo-Pacific region. The species, however, has now been found in the Western Atlantic, in the Florida Keys, US, and in Bahia de Todos Santos, Brazil.

Computational analyses on the structure-function relation in ion channels

Ion channels are protein molecules, embedded in the lipid bilayer of the cell membranes. They act as powerful sensing elements switching chemicalphysical stimuli into ion-fluxes. At a glance, ion channels are water-filled pores, which can open and close in response to different stimuli (gating), and one once open select the permeating ion species (selectivity). They play a crucial role in several physiological functions, like nerve transmission, muscular contraction, and secretion. Besides, ion channels can be used in technological applications for different purpose (sensing of organic molecules, DNA sequencing). As a result, there is remarkable interest in understanding the molecular determinants of the channel functioning. Nowadays, both the functional and the structural characteristics of ion channels can be experimentally solved. The purpose of this thesis was to investigate the structure-function relation in ion channels, by computational techniques. Most of the analyses focused on the mechanisms of ion conduction, and the numerical methodologies to compute the channel conductance. The standard techniques for atomistic simulation of complex molecular systems (Molecular Dynamics) cannot be routinely used to calculate ion fluxes in membrane channels, because of the high computational resources needed. The main step forward of the PhD research activity was the development of a computational algorithm for the calculation of ion fluxes in protein channels. The algorithm - based on the electrodiffusion theory - is computational inexpensive, and was used for an extensive analysis on the molecular determinants of the channel conductance. The first record of ion-fluxes through a single protein channel dates back to 1976, and since then measuring the single channel conductance has become a standard experimental procedure. Chapter 1 introduces ion channels, and the experimental techniques used to measure the channel currents. The abundance of functional data (channel currents) does not match with an equal abundance of structural data. The bacterial potassium channel KcsA was the first selective ion channels to be experimentally solved (1998), and after KcsA the structures of four different potassium channels were revealed. These experimental data inspired a new era in ion channel modeling. Once the atomic structures of channels are known, it is possible to define mathematical models based on physical descriptions of the molecular systems. These physically based models can provide an atomic description of ion channel functioning, and predict the effect of structural changes. Chapter 2 introduces the computation methods used throughout the thesis to model ion channels functioning at the atomic level. In Chapter 3 and Chapter 4 the ion conduction through potassium channels is analyzed, by an approach based on the Poisson-Nernst-Planck electrodiffusion theory. In the electrodiffusion theory ion conduction is modeled by the drift-diffusion equations, thus describing the ion distributions by continuum functions. The numerical solver of the Poisson- Nernst-Planck equations was tested in the KcsA potassium channel (Chapter 3), and then used to analyze how the atomic structure of the intracellular vestibule of potassium channels affects the conductance (Chapter 4). As a major result, a correlation between the channel conductance and the potassium concentration in the intracellular vestibule emerged. The atomic structure of the channel modulates the potassium concentration in the vestibule, thus its conductance. This mechanism explains the phenotype of the BK potassium channels, a sub-family of potassium channels with high single channel conductance. The functional role of the intracellular vestibule is also the subject of Chapter 5, where the affinity of the potassium channels hEag1 (involved in tumour-cell proliferation) and hErg (important in the cardiac cycle) for several pharmaceutical drugs was compared. Both experimental measurements and molecular modeling were used in order to identify differences in the blocking mechanism of the two channels, which could be exploited in the synthesis of selective blockers. The experimental data pointed out the different role of residue mutations in the blockage of hEag1 and hErg, and the molecular modeling provided a possible explanation based on different binding sites in the intracellular vestibule. Modeling ion channels at the molecular levels relates the functioning of a channel to its atomic structure (Chapters 3-5), and can also be useful to predict the structure of ion channels (Chapter 6-7). In Chapter 6 the structure of the KcsA potassium channel depleted from potassium ions is analyzed by molecular dynamics simulations. Recently, a surprisingly high osmotic permeability of the KcsA channel was experimentally measured. All the available crystallographic structure of KcsA refers to a channel occupied by potassium ions. To conduct water molecules potassium ions must be expelled from KcsA. The structure of the potassium-depleted KcsA channel and the mechanism of water permeation are still unknown, and have been investigated by numerical simulations. Molecular dynamics of KcsA identified a possible atomic structure of the potassium-depleted KcsA channel, and a mechanism for water permeation. The depletion from potassium ions is an extreme situation for potassium channels, unlikely in physiological conditions. However, the simulation of such an extreme condition could help to identify the structural conformations, so the functional states, accessible to potassium ion channels. The last chapter of the thesis deals with the atomic structure of the !- Hemolysin channel. !-Hemolysin is the major determinant of the Staphylococcus Aureus toxicity, and is also the prototype channel for a possible usage in technological applications. The atomic structure of !- Hemolysin was revealed by X-Ray crystallography, but several experimental evidences suggest the presence of an alternative atomic structure. This alternative structure was predicted, combining experimental measurements of single channel currents and numerical simulations. This thesis is organized in two parts, in the first part an overview on ion channels and on the numerical methods adopted throughout the thesis is provided, while the second part describes the research projects tackled in the course of the PhD programme. The aim of the research activity was to relate the functional characteristics of ion channels to their atomic structure. In presenting the different research projects, the role of numerical simulations to analyze the structure-function relation in ion channels is highlighted.

Primer registro de Greenidea ficicola Takahashi (Hemiptera: Aphididae) en Argentina

Se informa acerca de la presencia por vez primera de Greenidea ficicola Takahashi en la Argentina. El áfido fue encontrado sobre Ficus benjamina cv variegata en La Plata, Buenos Aires, en octubre de 2009. Se describe e ilustra la hembra vivípara áptera.

Primera cita de Essigella californica (Essig, 1909) (Hemiptera: Aphididae) en Argentina

Una búsqueda de pulgones sobre especies de Pinus se realizó en diferentes provincias de la Argentina. La identificación del material colectado permite citar por primera vez para este país Essigella californica (Essig), hallado en cinco provincias. Se presentan aspectos biológicos y morfológicos que permiten separar los tres géneros de la tribu Eulachnini (subfamilia Lachninae) presentes ahora en la Argentina: Cinara, Eulachnus y Essigella

Primer registro del daño de Leucothrips piercei (Morgan) (Thysanoptera: Thripidae) en cultivos de pimiento (Capsicum annuum L.) (Solanaceae) en la Argentina

Se cita por primera vez para la Argentina la presencia de Leucothrips piercei (Morgan) y se registra a Capsicum annuum L. como una nueva planta huésped. Se describen los caracteres diagnósticos y se detallan las características del daño ocasionado a las hojas de pimiento.

Primer registro en Argentina de Drepanosiphum oregonense Granovsky (Hemiptera: Aphididae) hallada sobre arces (Sapindaceae)

Se informa del hallazgo del áfido Drepanosiphum oregonense Granovsky (Hemiptera: Aphididae) sobre árboles de Acer pseudoplatanus y A. campestre (Sapindaceae) en Argentina lo que constituye el primer registro de la especie y del género Drepanosiphum Koch en Argentina y el segundo en Sudamérica.

Late Pliocene stratigraphic distribution of Globoconella taxa in the North Atlantic

Quantitative records of Globorotalia puncticulata and Globorotalia inflata, the last two members of the Globorotalia (Globoconella) lineage, obtained from North Atlantic sediments collected at DSDP Site 552, ODP Site 659 and ODP Site 665, are used to examine fluctuations in the biogeographic distribution of these species in the Late Pliocene between 3 and 2 Ma. Abundance data indicate that prior to the expansion of Northern Hemisphere glaciation at about 2.5 Ma, Gr. puncticulata was an important component of the planktonic foraminiferal fauna and had a geographic distribution ranging from 2°N to at least 56°N in the North Atlantic. A previously undescribed 6 chambered variant of Gr. puncticulata is found at both Sites 659 and 665. The stratigraphic distribution of this morphotype is restricted, first occurring at 2.9 Ma and then disappearing when glacial intensity increased at 2.75 Ma (isotope stage 110). Similar declines in Gr. puncticulata abundances occurred during glacial isotope stages 102, 100, and 98 immediately prior to the extinction of Gr. puncticulata during glacial isotope stage 96. It appears that this extinction event was latitudinally diachronous within the North Atlantic, occurring earliest in the north at Site 552 (2.453 Ma), then at Site 659 (2.443 Ma) and later still in the Site 665 equatorial record (2.438 Ma). At Site 665 the first record of Gr. inflata occurs during glacial isotope stage 94 (2.416 Ma), shortly after the extinction of Gr. puncticulata. In the mid latitude North Atlantic there was a 340,000 year period following the disappearance of Gr. puncticulata when the Globoconella lineage was absent (the Gr. inflata gap). The Gr. inflata population found in the equatorial Atlantic must therefore have been introduced from the South Atlantic, probably by the South Equatorial Current. Faunal records from Sites 552 and 659 show that it was not until glacial isotope stage 78 (2.10 Ma) that Gr. inflata became widely established in the North Atlantic. Prior to this large-scale migration event, there were two limited colonisation events during glacial isotope stages 86 and 82 when Gr. inflata populations reached as far as Site 659 in the eastern North Atlantic. These incursions are believed to be reflect the entrainment of Gr. inflata within South Atlantic Central Water and the northward subsurface transport of individuals to the coastal upwelling zone off northwest Africa. It seems likely that the same mechanism was responsible for the re-establishment of the Globoconella lineage in the North Atlantic at 2.10 Ma, but in this instance additional factors, such as enhanced glacial circulation patterns and ecological changes within planktonic foraminiferal faunas, resulted in the successful expansion of Gr. inflata across the North Atlantic and the Mediterranean.

AMS radiocarbon dates and pollen analysis of ODP Hole 175-1078C

ODP Site 1078 situated under the coast of Angola provides the first record of the vegetation history for Angola. The upper 11 m of the core covers the past 30 thousand years, which has been analysed palynologically in decadal to centennial resolution. Alkenone sea surface temperature estimates were analysed in centennial resolution. We studied sea surface temperatures and vegetation development during full glacial, deglacial, and interglacial conditions. During the glacial the vegetation in Angola was very open consisting of grass and heath lands, deserts and semi-deserts, which suggests a cool and dry climate. A change to warmer and more humid conditions is indicated by forest expansion starting in step with the earliest temperature rise in Antarctica, 22 thousand years ago. We infer that around the period of Heinrich Event 1, a northward excursion of the Angola Benguela Front and the Congo Air Boundary resulted in cool sea surface temperatures but rain forest remained present in the northern lowlands of Angola. Rain forest and dry forest area increase 15 thousand years ago. During the Holocene, dry forests and Miombo woodlands expanded. Also in Angola globally recognised climate changes at 8 thousand and 4 thousand years ago had an impact on the vegetation. During the past 2 thousand years, savannah vegetation became dominant.