Effect of stocking density on growth and yield of GIFT tilapia under rice-fish production system

The study was conducted at the Central Experimental Station, Philippine Rice Research Institute, Maligaya, Science City of Munoz, Nueva Ecija, Philippines during the wet season to determine the suitable stocking density(s) for better growth and yield of fish under rice-fish production systems. Recovery rate of GIFT tilapia in different stocking densities ranged from 75.74 to 83.47%. Among different treatments, rice +5,000 fingerlings/ha and rice +10,000 fingerlings/ha resulted in the highest recovery rate of 83.33% and 83.47%, respectively. The lowest recovery rate of 75.75% was obtained from rice +20,000 fingerlings/ha, but similar to that was obtained (78.56%) from rice +15,000 fingerlings/ha. Significantly higher rate of gain in body weight and that of specific growth rate were recorded in the treatment from rice +5,000 fingerlings/ha, while other treatments resulted in similar absolute and specific growth rate. Fish yield increased significantly with relatively higher stocking densities, but higher densities produced maximum number of smaller fishes and also lower recovery rate.

Influence of a fish bacterium Lactobacillus sp. on the production of swordtail xiphophorus helleri (Heckel 1848)

The influence of a fish gut bacterium Lactobacillus sp on the production of swordtail Xiphophorus helleri was studied for a period of one year. The Lactobacillus sp P21 produced bacteriocin-like inhibitory substance and exhibited wide spectrum of action against Aeromonas hydrophila, Bacillus spp, Pseudomonas spp and Citrobacter freundi in vitro. The growth performance of X. helleri reared in the presence of Lactobacillus P21 at 106/ml rearing water was better than the control. The total plate counts, total MRS agar counts and the counts of motile aeromonads, presumptive pseudomonads, lactose fermenters and lactose non-fermenters in the gut of probiotic group were comparatively low than the control. On day 60 the count of Lactobacillus sp P21 was observed to be log 5.28/g in the gut of X. helleri indicating colonization of this bacterium in the gastrointestinal tract. The fecundity of X. helleri was in the range of 9-134. On average, it produced from 39.42±18.72 fry/female in control group to 53.00±23.57 fry/female in probiotic group. The increase in average fecundity in probiotic group over the control group was about 25%. There existed significant difference between probiotic group and control in respect of average fecundity/female (p<0.02), average number of fry survived /female (p<0.006) and average number of fry dead/female (p<0.029). The results of the present study demonstrated that the rearing of X. helleri in probiotic-enriched water have growth inducing ability and favourably influenced the reproductive performance in terms of high fecundity, high fry survival, reduced fry mortality and reduced fry deformity.

Year-round and rotational freshwater prawn, Macrobrachium rosenbergii and paddy farming: soil quality, production and economics

A study was carried out at five upazillas (Dumuria, Fakirhat, Pirojpur sadar, Gopalgonj sadar and Kalia) of five southern districts of Khulna, Bagerhat, Pirojpur, Gopalgonj and Narail to understand the comparative production performance and any effect on soil quality in case of year-round golda (Macrobrachium rosenbergii), alternate prawn-paddy and year-round paddy farming. A direct interview of 55 farmers was taken, using pretested questionnaire, and soil samples were taken from selected farms before and after each crop. Among the five upazillas, farmers in Gopalgonj sadar and Kalia are not practicing year-round golda. Rotational golda-paddy farming has been recorded to result in maximum profit, with the highest of Tk. 310,912/ha/year in 2003 at Dumuria. Only paddy farming is less profitable than other two cropping patterns, irrespective of study sites. The nutrient status of soil in rotational golda and paddy farming has been found improved, compared to that of only golda or paddy farming.

Introducing tilapia (GIFT) with shrimp (Penaues monodon) in brackishwater rice-shrimp system: impact on water quality and production

Mixed rearing of tilapia (Genetically Improved Farmed Tilapia, GIFT) with shrimp (Penaeus monodon) in brackishwater rice-shrimp system was assessed for its impact on dry season's shrimp production. The experiment was conducted in pre-selected farmer's field located at Paikgacha Upazila of Khulna district and designed with three different densities (treatment) of GIFT, viz, 0.3, 0.4 and 0.5/m² with a constant stocking density of shrimp at 3/m². Each treatment had three replications. There had a set of control treatment where GIFT was not stocked. Results of the experiment revealed that tilapia did not exert any significant effect (p>0.05) on the water quality variables, even on survival rate of shrimp (p>0.05) under farm level condition in rice-shrimp rotational system, but a density dependent negative effect (P<0.05) on the growth of shrimp led apparently lower production rate of shrimp. Though tilapia provided the major augment of total production (p<0.05) in the respective treatments than in monoculture of shrimp, but not that of the economic return. However, economic loss due to sudden shrimp crop failure might be partially minimized by the tilapia crop.

Effects of different types of feeds on growth and production of tiger shrimp, Penaeus monodon at Bagerhat region, Bangladesh

An experiment was carried out in farmers' gher (shrimp farm) at Bagerhat sadar upazilla, Bagerhat to ascertain the effects of three different types of feeds on the production and economics of brackishwater shrimp, Penaeus monodon for a period of 120 days. There were three treatments such as T1 (BFRI dough feed containing of 30% fish meal, 10% protein conc., 10% soya meal, 15% mustard oil cake, 18% rice bran, 5% maize, 10% wheat flour, 1% oyster shell powder and 1% vitamin premix), T2 (Commercial diet Saudi-Bangla grower) and T3 (Saudi-Bangla special feed). Each treatment had two replicates and the stocking of shrimp in each gher was 3 nos/m². Water quality parameters did not differ significantly among the treatments except water depth. Average production and net return of shrimp in different treatments varied from 404.0 to 509.0 kg/ha and Tk. 56,493.99-Tk. 84,209.60, respectively. T2 showed significantly (p<0.05) the highest production and economic return. The result of the study implied that T2 is more suitable and economically viable than that of other treatments for shrimp farming.

Enhancement of fish production in a reservoir after partitioning by dikes through community participation

A reservoir of 70 acres was portioned by dikes into four manageable big ponds to get more production of fishes at Basurhat, Noakhali, Bangladesh under the supervision of local community through a society of 40 people ownership. Pangus (Pangasius hypophthalmus) @ 20,000/acre, and then fry and fingerlings of different types of fishes such as catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhina mrigala), grass carp (Ctenophmyngodon idella), bighead (Aristichthys nobili), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus cmpio) and rajpunti (Puntius gonionatus) @ 500/acre were stocked. Feed containing 25% protein was used two times daily and feed was adjusted fortnightly. After 8 months, all the fishes were weighed 0.80-2.10 kg except rajpunti (150-200 g) and tilapia (150-220 g), and a total of 25 ton of fish was harvested which was five times higher than the previous production under signal ownership. The production of fishes were increased after partitioning the lake with dikes due to proper management and control.

Suitability of commercially available Bacillus probiotics on growth, survival and production of black tiger shrimp (Penaeus monodon Fab.)

Different forms of Bacillus probiotics was assessed in the earthen ponds on tiger shrimp (Penaeus monodon) culture. The experiment was designed with three different treatments depending on the mode of application (T1=oral probiotics; T2=spreading probiotics and T3=oral+ spreading probiotics). The shrimp was cultured for 120 days with the stocking density of 6-PL/m².Oral probiotics in the respective ponds were supplied with feeds. Whereas, spreading probiotics was applied to the pond water during pond preparation at 30, 60 and 90 days of culture period. Results of the experiment revealed that, all forms of Bacillus probiotic had effective role to keep the culture environment friendly in terms of mineralization of organic matter, nitrogen and phosphorus content in bottom sediment; holding of water transparency in a congenial state, increasing the density of planktonic biomass and boosting the THB-Vibrio ratio in water and sediment with insignificance (p>0.05) difference between different treatments. Whilst, spreading form of Bacillus pro biotic showed higher weight gain (27.58±1.18g), survival rate (70.75±8.54%) and production (1167.66±109.62 kg/ha) and expected lower FCR (1.81 ±0.06) values with significant difference (p<0.01) with others methods of application, indicated its superiority in tiger shrimp culture.

Effects of stocking density on growth and production of GIFT (Oreochromis niloticus)

The study was carried out to assess the effects of stocking density on growth and production of GIFT for a period of 100 days. Three stocking densities were used 150, 200 and 250 fish/decimal; designated as treatment T1, T2 and T3 respectively having two replicates for each. Commercial pellet feeds were fed at the rate of 30% body weight up to first 10 days and then gradually it was readjusted to 22%, 18%, 15%, 12%, 10%, 8%, 6%, 5% and 4% respectively after every 10 days interval. The result showed that the fish in the treatment T1 stocked with the lowest stocking density (150 fish/dec) resulted in best individual weight gain (148.65g) followed by those in treatment T2 and T3 respectively. The specific growth rates (SGR) at every 10 days were ranged from 6.59 to 1.11 in different treatments during the experimental period. The food conversion ratio (FCR) values ranged between 1.82 to 2.03 with treatment T1 showing the lowest FCR. The survival rate ranged between 84 to 92%. Treatment T1 and treatment T2 showed significantly higher survival than Treatment T3. The fish production rate in treatment T1, T2 and T3 were 18.58, 23.87 and 26.78 kg/decimal respectively.

Feasibility study of biofuel production from freshwater fern, Azolla sp in Anzali Wetland

Production of bioethanol through acidic and enzymatic hydrolysis of aquatic Azolla sp., as a new source of bio-mass, has been performed, as a means to control increasing growth and reducing undesirable effects of this plant in Anzali lagoon. After sampling, drying and crushing, Azolla was hydrolyzed, using diluted acid and enzyme. Diluted acid hydrolysis was done using both autoclave and a high-pressure system (Batch Synth® Microwave synthesizer). The effects of temperature and time (in autoclave) and concentration of acid (in both) were compared. Cellubrix®, a ommercial cellulase source, was used for enzymatic hydrolysis process. The amounts of reducing sugars, glucose and furfural, released from hydrolyzate, were measured. To produce alcohol, Sacchromyces cerevisiae (to ferment sixcarbon sugars), Zygowilliopsis californica and Pichia stipitis (to ferment five-carbon and sixcarbon sugars) were used. Maximum amounts of glucose (4.83% w/w) and reducing sugars (14.15% w/w) were obtained using acid hydrolysis in autoclave. In the microwave oven, maximum glucose (5.04% w/w) and reducing sugars (13.27 w/w) were obtained at 180 and 200 °C, respectively. Under these conditions, maximum produced furfural was 1.54 g/L. The difference between amounts of furfural obtained from acid hydrolysis of Azolla in microwave oven compared to autoclave was statistically significant. Amounts of alcohol produced and its yields were 3.99 g/L and 33.13% for S. cerevisiae in 48 hours, 3.73 g/L and 30.45% for Pichia stipites in 48 hours, and 3.73 g/L and 30.45% for Z. californica in 24 hours after inoculation, respectively, with significant differences. Statistical comparison of results showed significant differences (P<0.05) in glucose production, at different conditions. Amounts of reducing sugars and glucose increased after optimization of levels of acid, time, and temperature. The overall optimum released sugar and glucose were obtained with 1.67% (w/v) acid using autoclave. Higher temperatures in microwave oven caused a significant increase (P<0.05) in furfural. Furfural severely inhibits fermentation. Hence, regarding the issues of energy consumption and time, amounts of inhibiting substances and sugar production, autoclave is found to be superior to the high temperature and pressure, generated in microwave oven, for hydrolyzing Azolla. Furthermore, given the amounts of Azolla in Anzali lagoon, it may be recommendable to use this plant as a biomass resource.

Effect of diets containing various protein and energy levels on some growth parameters, survival and the production of Astacus leptodactylus in different salinities

A factorial experiment was conducted for 60 days to determine of the response of Narrow clawed crayfish Astacus leptodactylus (average weight of 17±2.3 g) to diets containing various protein and energy levels. Nine diets containing three levels of protein (30, 35 and 40 %) and three levels of energy (300,370 and 450 kcal/100g) were formulated and prepared in this trial. Each diet also was used in two levels of salinity include 0 (fresh water) and 12 ppt(Caspian sea water). So this study was conducted with 18 treatments and triplicates random group of 5 crayfish per each 110-litre tank. Weight Gain, Feed conversion ratio (FCR), Protein Efficiency Ratio (PER), Net Protein Utilization (NPU), Daily Food Consumption (DFC), Survival (SVR) and body composition of tail-muscle meat of animal were determined. Comparing the growth parameters in response to interaction between protein, energy and salinity levels demonstrated that all growth parameters have difference between them significantly (p<0.05). Comparing between survival in fresh and Caspian Sea water showed difference significantly. Compare the body composition results indicate the greatest amount of protein absorption in diet number 2(30/370) on fresh water condition. Results from this study indicate that narrow clawed crayfish can be fed a practical diet containing 30% protein and 370 Kcal/100g on non-salinity water which is the optimize CP percentage for their producer’s profits.

A study of the constraints affecting ornamental fish production in Sri Lanka

Although Sri Lanka is endowed with favourable climatic conditions and resources for breeding and rearing ornamental fish for export, a considerable number of ornamental fish producers as well as exporters have given up the industry within a relatively short period of time. This study was conducted to understand the present status of the industry and to identify the problems that have caused these failures. The study was conducted from March to December in the year 2007 and covered Colombo, Kaluthara, Polonnaruwa, Negombo, Wattala, Rathnapura, Avissawella, Kandy, Kegalle, Padukka, and Gampaha areas, where ornamental fish culture is known to be popular. The survey was carried out by interviewing ornamental fish farmers using a structured questionnaire survey that was designed to elicit the required information. Most (75%) of those surveyed were identified as small scale farmers. A majority (56%) of them used only cement tanks for their culture activities. Only 47% of farmers had proper technical knowledge or training on fish culture while 42% directly supplied their fish products to the expo1iers. The most important constraints identified by the study were as follows: (1) the sale price offish not changing in keeping with the increase in the material costs of production - Feed, cement, sand, transport and labour - in recent years. (2) Difficulty to find export markets for newcomers to enter the export market. (3) Lack of quality brooders and information on the most suitable fish varieties for the different climatic and water conditions in different areas in the country (3) Feed availability and cost. (4) Lack of adequate knowledge and technical support with regard to disease control and water quality management. (5) Difficulty to survive in the off season. (6) Difficulty in obtaining credit for expansion and the lack of sufficient involvement of responsible authorities in overcoming all these identified constraints.

The study of artificial propagation efficiency and fingerlings production of Coregonus lavaretus

In order to study of the artificial propagation efficiency in white fish (Coregonus lavaretus) and its fingerlings producing in IRAN, a 9 mounts study project was been done which during it, the characteristics of the matures and brood stocks fishes, the condition of their natural and artificial propagation, and the characteristics of produced frys, were been studied. Throughout the total 82 pieces caught fishes during September til February 2003, 10 pieces of them were the female brood stocks which during the catch time did not have spouse. The study of these fishes showed that there was no significant correlation between their weight and their length. The most and the least absolute fecundity of these brood stocks were 19120 and 11496 respectively. The artificial propagation was been done by 5 males and 4 females broods took which 57602 ova, with 89/2% fertilization rate, earned from them. The incubation period prolonged 55 days in 8°c. At the end of the incubation, 23913 larvae released. So the artificial propagation efficiency was calculated 41/51% in this study. Yolk sack absorption prolonged 4 days. 3 different food treatment were considered for fry breeding which contain of Brachiouns plicatilis as live food, salmon starter food as commercial food, and the mixed of equal amounts of live and commercial foods as third treatment. For each treatment, 3 repeat has been considered. Breeding duration prolonged 13 weeks throughout this period, different characteristics of fry were been studied weekly. The breeding results showed that there was very significant correlation between the weight and the length of frys. However the live food provided better results in growth and survival rate of frys during breeding initial 6 weeks. More ever, commercial food, in some characteristics, provided more acceptable results in comparing the live food after sixth week. The results of this study project showed that the artificial propagation in whitefish is possible in IRAN and the producing of its frys in order to restocking or introducing this species to the other Iranian suitable water resources is executable. Based on the earned information from this study, the suitable time for natural spawning of whitefish in IRAN (Amirkabir dam lake) determined between 10th January til 20th February.

Managing environmental impacts in aquaculture

The article presents the environmental impacts of aquaculture and the ways to manage them. The environmental impacts include issues of recreation and aesthetics, and the usage of resources and discharge of nutrients and organic matter into the environment. Likewise, the results are also discussed, such as reduced production, disease outbreak in cultured and wild populations, and an increase of regulatory restrictions being placed on aquaculture operations.

The study and production of fish sauce from Caspian Sea lam fish with traditional fermentation method and microbe and enzyme adding method and identification of important bacteria

Fish sauce is a popular fermented product used in south Asian countries which is made from different small fishes in this research work it was attempted to produce fish sauce from kilka of the Caspian sea, the fish sauce was made from three models of kilka ,such as whole kilka , cooked whole kilka and dressed kilka , each of these models treated it four different fashions of fermentation such as:1- Traditional method, 2- Enzymatic method 3- Microbial method, 4- Mixture of enzyme and microb The results of this investigation showed that time of fermentation for the traditional method was six month, enzymatic method one month, microbial method 3 month and the mixture of enzyme and microb 1 month. The rate of fermentation was least for dressed Kilka, microbial and biochemical changes of Kilka fish sauce were evaluated, total bacterial count was 2.1-6.15 log cfu/ml total volatile nitrogen (TVN) in samples recorded was 250 mg /100g, the amount of protein varied between 10-13 percent, the name of commercial enzymes added was Protamex and Flavourzyme, the bacteria added was L act ob acillus and Pediococous, fish sauce containers fish and 20% salt, temperature of keeping for fermentation was 37 degree c for 6 month.

Aquaculture of the mangrove red snapper

Mangrove red snapper (Lutjanus argentimaculatus) is an important marketable species throughout the Indo-Pacific region. It is a euryhaline species; it can tolerate freshwater, brackishwater and marine water. Modular culture is the common system in snapper production in the Philippines. Stocking density is 4,000 fry per cropping; there could be 2 croppings per year. Brief details are given of investment costs and returns for culture in brackishwater pens.