Patterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha

Natural luteolysis involves multiple pulses of prostaglandin F2alpha (PGF) released by the nonpregnant uterus. This study investigated expression of 18 genes from five distinct pathways, following multiple low-dose pulses of PGF. Cows on Day 9 of the estrous cycle received four intrauterine infusions of 0.25 ml of phosphate-buffered saline (PBS) or PGF (0.5 mg of PGF in 0.25 ml of PBS) at 6-h intervals. A luteal biopsy sample was collected 30 min after each PBS or PGF infusion. There were four treatment groups: Control (n = 5; 4 PBS infusions), 4XPGF (4 PGF infusions; n = 5), 2XPGF-non-regressed (2 PGF infusions; n = 5; PGF-PBS-PGF-PBS; no regression after treatments), and 2XPGF-regressed (PGF-PBS-PGF-PBS; regression after treatments; n = 5). As expected, the first PGF pulse increased mRNA for the immediate early genes JUN, FOS, NR4A1, and EGR1 but unexpectedly also increased mRNA for steroidogenic (STAR) and angiogenic (VEGFA) pathways. The second PGF pulse induced immediate early genes and genes related to immune system activation (IL1B, FAS, FASLG, IL8). However, mRNA for VEGFA and STAR were decreased by the second PGF infusion. After the third and fourth PGF pulses, a distinctly luteolytic pattern of gene expression was evident, with inhibition of steroidogenic and angiogenic pathways, whereas, there was induction of pathways for immune system activation and production of PGF. The pattern of PGF-induced gene expression was similar in corpus luteum not destined for luteolysis (2X-non-regressed) after the first PGF pulse but was very distinct after the second PGF pulse. Thus, although the initial PGF pulse induced mRNA for many pathways, the second and later pulses of PGF appear to have set the distinct pattern of gene expression that result in luteolysis.

Actively transcribed GAL genes can be physically linked to the nuclear pore by the SAGA chromatin modifying complex

Recent work has demonstrated that some actively transcribed genes closely associate with nuclear pore complexes (NPC) at the nuclear periphery. The Saccharomyces cerevisiae Mlp1 and Mlp2 proteins are components of the inner nuclear basket of the nuclear pore that mediate interactions with these active genes. To investigate the physical link between the NPC and active loci, we identified proteins that interact with the carboxyl-terminal globular domain of Mlp1 by tandem affinity purification coupled with mass spectrometry. This analysis led to the identification of several components of the Spt-Ada-Gcn5-acetyltransferase ( SAGA) histone acetyltransferase complex, Gcn5, Ada2, and Spt7. We utilized co-immunoprecipitation and in vitro binding assays to confirm the interaction between the Mlp proteins and SAGA components. Chromatin immunoprecipitation experiments revealed that Mlp1 and SAGA components associate with the same region of the GAL promoters. Critically, this Mlp-promoter interaction depends on the integrity of the SAGA complex. These results identify a physical association between SAGA and the NPC, and support previous results that relied upon visualization of GAL loci at the nuclear periphery by microscopy ( Cabal, G. G. Genovesio, A., Rodriguez-Navarro, S., Zimmer, C., Gadal, O., Lesne, A., Buc, H., Feuerbach- Fournier, F., Olivo-Marin, J.-C., Hurt, E. C., and Nehrbass, U. ( 2006) Nature 441, 770-773). We propose that a physical interaction between nuclear pore components and the SAGA complex can link the actively transcribed GAL genes to the nuclear pore.

Alternative Transfers to the NEOs 99942 Apophis, 1994 WR12, and 2007 UW1 via Derived Trajectories from Periodic Orbits of Family G

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de seis variedades de uvas sem sementes no Submédio São Francisco

This work aimed to study aspects related to vegetative and productive development of plants and characteristics and chemical components of bunches of six seedless grape varieties at environmental conditions of São Francisco river's valley, Northeastern Brazil. The experiment was carried out during 1997 and 1998. The varieties tested were grafted on IAC 572 ('Jales') rootstock. The experimental model utilized was totally randomized, and with parcels subdivided into different production cycles. The results showed significative differences among varieties at different pruning times. The average weight of bunches varied from 164.8 g for 'Marroo Seedless' and 203.5 g for 'Beauty Seedless'. The average diameter of berries was superior to 15.7 mm for all of the varieties. The total soluble solids varied from 14.05°Brix in 'Canner' to 19.6°Brix in 'Venus'. The total titratable acidity (ATT) was lower than 0.91 g tartaric acid/100 mL of juice. The ratio SST/ATT varied from 19.05 in 'Beauty Seedless' to 28.57 in 'Vênus'. 'Vênus' and 'Marroo Seedless' were the most productive varieties, with the average annual production of 24 ton/ha and 20 ton/ha, respectively.

Interplanetary natural routes via Moon and Lagrangiam points L1 and L2

Swing-by techniques are extensively used in interplanetary missions to minimize fuel consumption and to raise payloads of spaceships. The effectiveness of this type of maneuver has been proven since the beginning of space exploration. According to this premise, we have explored the existence of a natural and direct links between low Earth orbits and the lunar sphere of influence, to obtain low-energy interplanetary trajectories through swing-bys with the Moon and the Earth. The existence of these links are related to a family of retrograde periodic orbits around the Lagrangian equilibrium point L1 predicted for the circular, planar, restricted three-body Earth-Moon-particle problem. The trajectories in these links are sensitive to small disturbances. This enables them to be conveniently diverted reducing so the cost of the swing-by maneuver. These maneuvers allow us a gain in energy sufficient for the trajectories to escape from the Earth-Moon system and to stabilize in heliocentric orbits between the Earth and Venus or Earth and Mars. On the other hand, still within the Earth sphere of influence, and taking advantage of the sensitivity of the trajectories, is possible to design other swing-bys with the Earth or Moon. This allows the trajectories to have larger reach, until they can reach the orbit of other planets as Venus and Mars.(3σ)Broucke, R.A., Periodic Orbits in the Restricted Three-Body Problem with Earth-Moon Masses, JPL Technical Report 32-1168, 1968.

Mulheres recipientes: recortes poéticos do universo feminino nas artes visuais

Pós-graduação em Artes - IA

Consumir e descartar: análise da cobertura da Folha de S. Paulo e Jornal da Cidade no caso da proibição da distribuição das sacolas pláticas nos supermercados do Estado de São Paulo.

Pós-graduação em Comunicação - FAAC

“Um brinde aos assinantes!”: os Almanaques do jornal O Estado de S. Paulo (1896, 1916, 1940)

Pós-graduação em História - FCLAS

Influência do clareamento dental na alteração de cor, opacidade e fluorescência de diferentes resinas compostas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de diferentes meios de imersão na emissão fluorescente de resinas compostas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uma escola (in)visível: memórias de professoras negras em Porto Velho no início do século XX

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O teatro do mundo no mundo do teatro: a obra de Paulo Corrêa de Oliveira

Pós-graduação em Estudos Literários - FCLAR

Poder local e a gestão da educação municipal no contexto de Altamira-Pará

Com base nas discussões que tomam como eixo central a temática a ressignificação do poder local, enquanto força mobilizadora, e sua relação com a gestao da educação municipal, esta Dissertação busca problematizar se as definições institucionais e as práticas efetivadas pela Secretaria de Educação do Município de Altamira-PA tem contribuído para o fortalecimento do poder local, a fim de compreender ate que ponto o processo educativo possibilita a formação e inserção de agentes sociais nos processos de democratização da sociedade. Tendo como corpus de investigação a gestão da política educacional instituída nessa localidade, denominada de Programa Rede Vencer (antiga Escola Campeã), desenvolvida em parceria com o Instituto Ayrton Senna, avalia-se as estratégias e práticas propostas pela SEMEC no que tange a participação e o protagonismo da comunidade altamirense, examinando, para tanto, a natureza das redes relacionais constituídas entre esse orgão e os atores e segmentos sociais. Ao estabelecer uma articulação entre a metodologia das redes sociais e alguns princípios da análise do discurso, esta pesquisa identifica percepções e posicionamentos contraditórios em relações ao sentido de governança democrática e de descentralizacao e autonomia, bem como ao envolvimento da população nos processos de deliberaties e de partilha de poder, de modo que as definições instituídas pela Secretaria de Educação revelam o caráter centralizante na forma de gerir a política educacional ao ignorar a comunidade escolar e externa em sua definições e implementação. Este trabalho evidencia, ainda, que a gestão da educação assenta-se em um modelo racional/burocrático, fundamentado no controle dos resultados de desempenhos, o que favorece a concentração das decisões no ambito da SEMEC e da própria Prefeitura Municipal e, conseqüentemente, o afastamento e alheamento dos sujeitos escolares e da sociedade. Por fim, constata-se que o processo educativo, em sua dimensões curricular e administrativa, ainda não tem ainda proporcionado a formação socio-política dos sujeitos, como também nao tem contribuído para a criação de canais participativos com vistas a conquistar a democratização do poder local em Altamira.

As hagiografias como instrumentos de difusão do cristianismo católico nos meios rurais da Espanha visigótica

Pós-graduação em História - FCLAS