Immunolocalization of myosin Va in the developing nervous system of embryonic chicks

Myosins are molecular motors associated with the actin cytoskeleton that participate in the mechanisms of cellular motility. During the development of the nervous system, migration of nerve cells to specific sites, extension of growth cones, and axonal transport are dramatic manifestations of cellular motility. We demonstrate, via immunoblots, the expression of myosin Va during early stages of embryonic development in chicks, extending from the blastocyst period to the beginning of the fetal period. The expression of myosin Va in specific regions and cellular structures of the nervous system during these early stages was determined by immunocytochemistry using a polyclonal antibody. Whole mounts of chick embryos at 24-30-h stages showed intense immunoreactivity of the neural tube in formation along its full extent. Cross-sections at these stages of development showed strong labeling in neuroepithelial cells at the basal and apical regions of the neural tube wall. Embryos at more advanced periods of development (48h and 72 h) showed distinctive immunolabeling of neuroepithelial cells, neuroblasts and their cytoplasmic extensions in the mantle layer of the stratified neural tube wall, and neuroblasts and their cytoplasmic extensions in the internal wall of the optic cup, as well as a striking labeling of cells in the apparent nuclei of cranial nerves and budding fibers. These immunolocalization studies indicate temporal and site-specific expression of myosin Va during chick embryo development, suggesting that myosin Va expression is related to recruitment for specific cellular tasks.

Contribución a la modelización de la eliminación de residuos sólidos por vía marina

Doctorado en Ciencias del Mar

La simulación del equilibrio general como vía de valoración de políticas medioambientales en el sector eléctrico canario

[ES] La necesidad de reducir emisiones de CO2, consecuencia del Protocolo de Kyoto, hace que la intervención estatal sea necesaria para forzar al mercado a asumir los costes sociales y medioambientales de la producción eléctrica actual. Una vía de acción es el uso de políticas que promuevan sistemas de producción más limpios: un mayor uso de energías renovables. Con el fin de minimizar el coste de cambio de sistema es conveniente un estudio ex-ante de las políticas aplicables. El alto coste que la insularidad repercute al sistema eléctrico convierte a las Islas en un escenario privilegiado para su estudio.