503 resultados para Unfolding


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O artigo em questão estabelece reflexões em torno do tema do trabalho na região missioneira gaúcha, tomando como referência o legado das missões jesuítico-guaranis e seus desdobramentos no contemporâneo, a partir das memórias das pessoas que vivem naquela porção do Estado. Para tanto, o artigo se baseia numa pesquisa de campo de cerca de um ano, que teve como um dos interesses etnográficos a tentativa de compreender os processos de transformação das paisagens na região através das ações técnico-culturais relacionadas ao universo do trabalho.

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Pós-graduação em Agronomia - FEIS

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Pós-graduação em Ciências Sociais - FCLAR

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Música - IA

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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Streblin, a serine proteinase from plant Streblus asper, has been used to investigate the conformational changes induced by pH, temperature, and chaotropes. The near/far UV circular dichroism activities under fluorescence emission spectroscopy and 8-aniline-1-naphthalene sulfonate (ANS) binding have been carried out to understand the unfolding of the protein in the presence of denaturants. Spectroscopic studies reveal that streblin belongs to the alpha+beta class of proteins and exhibits stability towards chemical denaturants, guanidine hydrochloride (GuHCl). The pH-induced transition of this protein is noncooperative for transition phases between pH 0.5 and 2.5 (midpoint, 1.5) and pH 2.5 and 10.0 (midpoint, 6.5). At pH 1.0 or lower, the protein unfolds to form acid-unfolded state, and for pH 7.5 and above, protein turns into an alkaline denatured state characterized by the absence of ANS binding. At pH 2.0 (1M GuHCl), streblin exists in a partially unfolded state with characteristics of amolten globule state. The protein is found to exhibit strong and predominant ANS binding. In total, six different intermediate states has been identified to show protein folding pathways.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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This paper aims to present a study of the social relationships around the production, circulation and the eventual possibility of appropriation of knowledge in social systems of the pre-modern world and in a social organization of Western capitalist modernity. In this specific universe of relationships, it was also important to noticed that there is a paradoxical role of rationality that served to the emancipation of instruments of control’s knowledge of pre-modern period as in the same time it was favored by the construction of new bonds that were made in the Western modernity through the modern legal system, inserting the knowledge in the domain of the economy and private logic of the capitalist’s system through intellectual property. This research was made from a review of the literature, using specialized books of the theme. It was concluded that there is a paradox in the social relationships about the knowledge, whose cause is related to the unfolding of rationality in the development of Western civilization.

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This paper presents some of the territories occupied by the black community in the city of Rio Claro in the course of its history and check through their interpretations of cultural, emergence, unfolding and current use. The identification of changes in those territories was through oral history, building on the views and stories of their own community, literature review, data collection 'in situ' through research and observation of participants and resource utilization of the global network of information. It is justified by the fundamental importance of recording information in the history of the land and built space in the black community of Rio Claro, since the most part, as well as throughout the Brazilian territory, has no documentation and remains alive in the memory and the stories within the past communities, so each time you die an old man also lost part of the history of our nation