979 resultados para Stern-Volmer quenching


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El archivo tiene un estatus complejo y ambivalente. Puede ser un instrumento al servicio del poder y también ser albergue de conocimiento potencialmente emancipador capaz de desestabilizar verdades establecidas. Este trabajo propone recorrer e interrogar los usos que algunas intervenciones del arte moderno y contemporáneo realizaron con el soporte archivo, pensado antes que como repositorio inmóvil de saberes, discursos y conocimientos, como una norma que regula un tráfico de bienes simbólicos. Mi lectura intentará reconocer al archivo como un soporte contingente, mutante y dinámico, y recorrer algunos de los usos que el arte moderno y contemporáneo, en particular la fotografía y algunas zonas adyacentes han realizado con él

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Fil: Sánchez, Leandro Enrique. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales (UNLP-CONICET); Argentina.

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Fil: Sánchez, Leandro Enrique. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales (UNLP-CONICET); Argentina.

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El archivo tiene un estatus complejo y ambivalente. Puede ser un instrumento al servicio del poder y también ser albergue de conocimiento potencialmente emancipador capaz de desestabilizar verdades establecidas. Este trabajo propone recorrer e interrogar los usos que algunas intervenciones del arte moderno y contemporáneo realizaron con el soporte archivo, pensado antes que como repositorio inmóvil de saberes, discursos y conocimientos, como una norma que regula un tráfico de bienes simbólicos. Mi lectura intentará reconocer al archivo como un soporte contingente, mutante y dinámico, y recorrer algunos de los usos que el arte moderno y contemporáneo, en particular la fotografía y algunas zonas adyacentes han realizado con él

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El archivo tiene un estatus complejo y ambivalente. Puede ser un instrumento al servicio del poder y también ser albergue de conocimiento potencialmente emancipador capaz de desestabilizar verdades establecidas. Este trabajo propone recorrer e interrogar los usos que algunas intervenciones del arte moderno y contemporáneo realizaron con el soporte archivo, pensado antes que como repositorio inmóvil de saberes, discursos y conocimientos, como una norma que regula un tráfico de bienes simbólicos. Mi lectura intentará reconocer al archivo como un soporte contingente, mutante y dinámico, y recorrer algunos de los usos que el arte moderno y contemporáneo, en particular la fotografía y algunas zonas adyacentes han realizado con él

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Fil: Sánchez, Leandro Enrique. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación. Instituto de Investigaciones en Humanidades y Ciencias Sociales (UNLP-CONICET); Argentina.

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GaN and InGaN nanocolumns of various compositions are studied by room-temperature photoluminescence (PL) under different ambient conditions. GaN nanocolumns exhibit a reversible quenching upon exposure to air under constant UV excitation, following a t−1/2 time dependence and resulting in a total reduction of intensity by 85–90%, as compared to PL measured in vacuum, with no spectral change. This effect is not observed when exposing the samples to pure nitrogen. We attribute this effect to photoabsorption and photodesorption of oxygen that modifies the surface potential bending. InGaN nanocolumns, under the same experimental conditions do not show the same quenching features: The high-energy part of the broad PL line is not modified by exposure to air, whereas a lower-energy part, which does quench by 80–90%, can now be distinguished. We discuss the different behaviors in terms of carrier localization and possible composition or strain gradients in the InGaN nanocolumns.

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The fluorescence of a polyanionic conjugated polymer can be quenched by extremely low concentrations of cationic electron acceptors in aqueous solutions. We report a greater than million-fold amplification of the sensitivity to fluorescence quenching compared with corresponding “molecular excited states.” Using a combination of steady-state and ultrafast spectroscopy, we have established that the dramatic quenching results from weak complex formation [polymer(−)/quencher(+)], followed by ultrafast electron transfer from excitations on the entire polymer chain to the quencher, with a time constant of 650 fs. Because of the weak complex formation, the quenching can be selectively reversed by using a quencher-recognition diad. We have constructed such a diad and demonstrate that the fluorescence is fully recovered on binding between the recognition site and a specific analyte protein. In both solutions and thin films, this reversible fluorescence quenching provides the basis for a new class of highly sensitive biological and chemical sensors.

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Collectively, the xanthophyll class of carotenoids perform a variety of critical roles in light harvesting antenna assembly and function. The xanthophyll composition of higher plant photosystems (lutein, violaxanthin, and neoxanthin) is remarkably conserved, suggesting important functional roles for each. We have taken a molecular genetic approach in Arabidopsis toward defining the respective roles of individual xanthophylls in vivo by using a series of mutant lines that selectively eliminate and substitute a range of xanthophylls. The mutations, lut1 and lut2 (lut = lutein deficient), disrupt lutein biosynthesis. In lut2, lutein is replaced mainly by a stoichiometric increase in violaxanthin and antheraxanthin. A third mutant, aba1, accumulates normal levels of lutein and substitutes zeaxanthin for violaxanthin and neoxanthin. The lut2aba1 double mutant completely lacks lutein, violaxanthin, and neoxanthin and instead accumulates zeaxanthin. All mutants were viable in soil and had chlorophyll a/b ratios ranging from 2.9 to 3.5 and near wild-type rates of photosynthesis. However, mutants accumulating zeaxanthin exhibited a delayed greening virescent phenotype, which was most severe and often lethal when zeaxanthin was the only xanthophyll present. Chlorophyll fluorescence quenching kinetics indicated that both zeaxanthin and lutein contribute to nonphotochemical quenching; specifically, lutein contributes, directly or indirectly, to the rapid rise of nonphotochemical quenching. The results suggest that the normal complement of xanthophylls, while not essential, is required for optimal assembly and function of the light harvesting antenna in higher plants.

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We have developed a simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY® FL fluorescence was quenched by its interaction with a uniquely positioned guanine. This approach makes use of an oligonucleotide probe or primer containing a BODIPY® FL-modified cytosine at its 5′-end. When such a probe was hybridized with a target DNA, its fluorescence was quenched by the guanine in the target, complementary to the modified cytosine, and the quench rate was proportional to the amount of target DNA. This widely applicable technique will be used directly with larger samples or in conjunction with the polymerase chain reaction to quantify small DNA samples.