Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches

Three cDNA sequences encoding four SNARE (N-ethylmaleimide-sensitive fusion protein attachment protein receptors) motifs were cloned from sea perch, and the deduced peptide sequences were analyzed for structural prediction by using 14 different web servers and softwares. The "ionic layer" structure, the three dimensional extension and conformational characters of the SNARE 7S core complex by using bioinformatics approaches were compared respectively with those from mammalian X-ray crystallographic investigations. The result suggested that the formation and stabilization of fish SNARE core complex might be driven by hydrophobic association, hydrogen bond among R group of core amino acids and electrostatic attraction at molecular level. This revealed that the SNARE proteins interaction of the fish may share the same molecular mechanism with that of mammal, indicating the universality and solidity of SNARE core complex theory. This work is also an attempt to get the protein 3D structural information which appears to be similar to that obtained through X-ray crystallography, only by using computerized approaches. (C) 2007 Elsevier Ltd. All rights reserved.

CFX与USAERO的水下机器人操纵性仿真计算研究

研究了借助计算流体力学软件CFX与USAERO对水下机器人的操纵性进行仿真计算的方法,并将仿真计算结果与模型试验结果对比,验证仿真计算的可行性,指出仿真计算的适用范围.该方法可以对某些现实中难以进行的操纵性试验进行仿真计算,对于研究水下机器人的操纵性尤其是在方案设计阶段的操纵性设计和降低试验成本有一定的现实意义.

油气运聚动力学的模拟分析——以准噶尔盆地为例

The petroleum migration, happening in the geologic past, is the very important and complex dynamic processes in the petroleum systems. It plays a linking role among all static factors in a system. The accumulation is in fact the result of the petroleum migration. For the petroleum geology, the dynamics research of the petroleum migration refers to the mechanism and process research, as well as the use of the quantitative methods. In this thesis, combining with the qualitative analysis and quantitative modeling, the author manages to discuss theoretically some key problems dealing with migration processes, which have not been solved yet, and to apply the studied results in petroleum system analysis in actual basins. The basin analysis offers the base of the numerical modeling for geological phenomena occurring in sedimentary basins, that consists of the sedimentary facies analysis, the section reconstructing technique, eroded thickness estimating, etc. The methods to construct the geologic model, which is needed in the research of oil and gas migration and accumulation, are discussed. The basin analysis offers also the possibility for the latter modeling works to get and select the parameters, such as stratum's thickness, age, stratigraphy etc. Modeling works were done by using two basin modeling softwares: Basin_Mod and TPC_Mod. The role of compaction during the secondary migration and the heterogeneity of migrating paths within the clastic carrier are modeled. And the conclusions were applied in the migration studies in the Jungaer Basin, lying on the Northwest part of the China. To construct a reliable migration model, the author studied the characteristics of the sedimentation, the pore fluid pressure evolution, as well as the distribution and the evolution of fluid potential, following the tectonic evolution of the Jungaer Basin. The geochemical prospecting results were used to evidence and to calibrate the migration processes: the oil-source correlation, the distribution of the properties of oil, gas and water. Finally, two important petroleum systems, Permian one and Jurassic one were studied and identified, according, principally, to the studies on the petroleum migration within the Jungaer Basin. Since the oil, as well as the gas, moves mainly in separate phase during the secondary migration, their migrating behaviors would be determined by the dynamics conditions of migration, including the driving forces and pathways. Based on such a consideration, the further understandings may be acquired: the roles played by permeable carriers and low-permeable source rock would be very different in compaction, overpressure generation, petroleum migration, and so on. With the numerical method, the effect of the compaction on the secondary migration was analyzed and the results show that the pressure gradient and the flux resulted from compaction are so small that could be neglected by comparing to the buoyancy of oil. The main secondary migration driving forces are therefore buoyancy and capillary within a hydrostatic system. Modeling with the commercial software-Basin_Mod, the migration pathways of petroleum in clastic carriers seem to be inhomogeneous, controlled by heterogeneity of the driving force, which in turn resulted from the topography of seals, the fabrics and the capillary pressure of the clastic carriers. Furthermore, the direct and indirect methods to study fault-sealing properties in the course of migration were systemically summarized. They may be characterized directly by lithological juxtaposition, clay smear and diagenesis, and indirectly the comparing the pressures and fluid properties in the walls at two apartments of a fault. In Jungaer Basin, the abnormal pressures are found in the formations beneath Badaowan or Baijantan Formation. The occurrence of the overpressure seems controlled by the stratigraphy. The rapid sedimentation, tectonic pressuring, clay sealing, chemical diagensis were considered as the principal pressuring mechanisms. The evolution of fluid pressure is influenced differently at different parts of the basin by the tectonic stresses. So the basin appears different pressure evolution cycles from each part to another during the geological history. By coupling the results of thermal evolution, pressure evolution and organic matter maturation, the area and the period of primary migration were acquired and used to determine the secondary migration time and range. The primary migration in Fengcheng Formation happened from latter Triassic to early Jurassic in the main depressions. The main period of lower-Wuerhe Formation was at latter Jurassic in Changji, Shawan and Pen-1-jing-xi Depression, and at the end of early Cretaceous in Mahu Depression. The primary migration in Badaowan and Sangonghe Formation is at the end of early-Cretaceous in Changji Depression. After then, the fluid potential of oil is calculated at the key time determined from area and time of the primary migration. Generally, fluid potential of oil is high in the depressions and low at the uplifts. Synthetically, it is recognized that the petroleum migration in the Jungaer Basin is very complex, that leads us to classify the evolution of petroleum systems in Northwestern China as a primary stage and a reformed one. The remigration of accumulated petroleum, caused by the reformation of the basin, results in the generation of multiple petroleum systems. The faults and unconformities are usually the linkers among the original petroleum systems. The Permian petroleum system in Jungaer Basin is such a multiple petroleum system. However, the Jurassic petroleum system stays still in its primary stage, thought the strong influences of the new tectonic activities.

大港千米桥地区综合地质地球物理研究

Qianmiqiao buried hill, which is a high-yield burial hill pool, was discovered at Dagang oilfield in 1998. To employ the integrated geological and geophysical research at Qianmiqiao area, it is very valuable and meaningful for the petroleum exploration of Bohai Bay Basin and even the whole country. Based on the previous results, this paper is carried out from the research on Huanghua depression, following the law, i.e. the deep part constrains the shallow, the regional constrains the local, takes the geophysical research in Qianmiqiao oilfield, discusses the formation history of burial hills, burial history, thermal history, the generated and expelling history of hydrocarbon, and migration characteristics, probes into the formation of burial hill pool. This paper uses the gravity and magnetic methods which are based on potential field, with natural sources, configures the inner structure of the earth according to the difference in the density and magnetism of the rock. The geophysical characteristics of Dagang oil field is that it is an area with positive Buge gravity anomal. The upheaval of Moho boundary is in mirror symmetry with the depression of the basin's basement. The positive and negative anomaly distributein axis symmetry, and the orientation is NNE. The thickness of the crust gradually reduces from west to east, from land to sea. The depth gradient strip of Curie surface is similar to Moho boundary, whereas their local buried depth is different. Local fractures imply that the orientation of base rock fractures is NNE-NE, and the base rock is intersected by the fractures of the same/ later term, whose orientation is NW, so the base rock likes rhombic mosaic. The results of tomography show that there exists significant asymmetry in vertical and horizontal direction in the velocity configuration of Huanghua depression. From Dezhou to Tianjin, there exits high-speed block, which extends from south to north. The bottom of this high-speed block is in good agreement with the depth of Moho boundary. Hence we can conclude that the high-speed block is actually the crystal basement. According to seismic data, well data and outcrop data, Huanghua depression can be divided into four structure layers, i.e. Pi,2-T, Ji,2-K, E, N-Q. Qianmiqiao burial hills undergo many tectonic movement, where reverse faults in developed in inner burial hill from Indosinian stage to Yanshanian stage, the normal faults extended in Himalayan stage. Under the influence of tectonic movements, the burial hills show three layers, i.e. the reverse rushing faults in buried hills, paleo-residual hill, and extended horst block. The evolution of burial hills can be divided into four stages: steady raising period from Calenonian to early Hercynian, rushing brake drape period from Indosinian to middle Yanshanian, block tilting period in early Tertiary, and heating depression period from late Tertiary to Quaternary. The basin modeling softwares BasinMod 1-D and Basin 2-D, which are made by PRA corporation, are used in this paper, according to the requirement, corresponding geological model is designed. And we model the burial history, thermal history, hydrocarbon generation and hydrocarbon expelling history of Qianmiqiao area. The results show that present bury depth is the deepest in the geological history, the sedimentary rate of Tertiary is highest and its rising rate of temperature rate is higher. During sedimentary history, there is no large erosion, and in the Tertiary, the deeper sediment was deposited in large space, therefore it is in favor of the conservation and transformation of oil and gas. The thermal research shows that the heat primarily comes from basement of the basin, present geotherm is the highest temperature in the geological history. Major source rock is the strata of ES3, whose organic is abundant, good-typed, maturative and of high-expulsive efficiency. The organic evolution of source rock of O has come to the overmature stage, the evolving time is long and the source rock can be easily destroyed. Therefore it is more difficult for the O formation source rock to form the huge accumulation of oil and gas than Es3 formation. In the research of oil assembling, we first calculated the characteristics of the fluid pressure of single well, then analyzed the distribution of the surplus fluid pressure of each formation and profile, and probe the first hydrocarbon migration situation and the distribution of pressure system of buried hill pool. In every formation, the pressure system of each burial hill has its own characteristics, e.g. high pressure or low pressure. In the research of secondary migration, the fluid potential is calculated while the relative low potential area is figured out. In Qianmiqiao area, the west margin faults have the low potential, and hence is the favorable reconnoiter belt.

油藏数值模拟中的网格粗化和细化技术研究

The grid is a foundation of reservoir description and reservoir simulation. The scale of grid size is vital influence for the precision of reservoir simulation the gridding of reservoir parameters require reasonable interpolation method with computing quickly and accurately. The improved distant weighted interpolation method has many properties, such as logical data points selection, exact interpolation, less calculation and simply programming, and its application can improve the precision of reservoir description and reservoir simulation. The Fractal geologic statistics describes scientifically the distribution law of various geological properties in reservoir. The Fractal interpolation method is applied in grid interpolation of reservoir parameters, and the result more accorded with the geological property and configuration of reservoir, and improved the rationality and quality of interpolation calculation. Incorporating the improved distant weighted interpolation method with Fractal interpolation method during mathematical model of grid-upscaling and grid-downscaling, the softwares of GROUGH(grid-upscaling) and GFINE (grid-downscaling) were developed aiming at the questions of grid-upscaling and grid-downscaling in reservoir description and reservoir simulation. The softwares of GROUGH and GFINE initial applied in the research of fined and large-scale reservoir simulation. It obtained fined distribution of remaining oil applying grid-upscaling and grid-downscaling technique in fined reservoir simulation of Es21-2 Shengtuo oilfield, and provided strongly and scientific basis for integral and comprehensive adjustment. It's a giant tertiary oil recovery pilot area in the alkaline/surfactant/polymer flooding pilot area of west district of Gudao oilfield, and first realized fined reservoir simulation of chemical flooding using grid-upscaling and grid-downscaling technique. It has wide applied foreground and significant research value aiming at the technique of grid-upscaling and grid-downscaling in reservoir description and reservoir simulation.

Webmapping desenvolvido em tecnologia Flex para análises e monitoramento de obras de infraestrutura.

Buscando o aprimoramento das análises de imagens, maior segurança e contínua disponibilização de imagens de satélite para monitoramento orbital das obras do Programa de Aceleração do Crescimento (PAC) do governo federal, uma nova interface de webmapping foi desenvolvida pelas áreas de Gestão Territorial Estratégica (GTE) e de Tecnologia da Informação (GTI) da Embrapa Monitoramento por Satélite utilizando a linguagem Flex. Essa nova interface é complementar à desenvolvida e apresentada em Carvalho (2009). Flex é uma linguagem de programação baseada em Flash para a criação de aplicações web do tipo Internet Rica (da sigla em inglês RIA ? Rich Internet Application), que têm características e funcionalidades de softwares tradicionais do tipo aplicativo.

Infra estrutura da agricultura de precisao no Brasil.

Profissionais em agricultura de precisao (projeto, coordenacao, etc); Empresas, equipamentos e servicos para agricultura de precisao; Geoestatistica (softwares, livros e sites na internet); Outros sites para busca em agricultura de precisao; Trabalho publicados em agricultura de precisao.

Uma solução para melhoria de desempenho para servidores de correio eletrônico com antispam.

Procmail. Softwares antispams classificatórios. Softwares antispams do tipo desafio/resposta. Solução proposta. Configuração de filtros em procmailrc para evitar spams.

Tutorial SGP: Sistemas Gestor de Publicações da Embrapa Informática Agropecuária. Versão 2.0

O Sistema Gestor de Publicações - SGP é uma ferramenta que tem como objetivo principal registrar todos os passos do processo das publicações internas da Embrapa Informática Agropecuária. Baseado nos softwares de submissão mais utilizados pela comunidade científica internacional, contempla as funções de submissão de documentos, acompanhamento, emissão de relatórios e pareceres, além do acompanhamento, pelo autor, do andamento do trabalho de seu trabalho. Todas suas funções serão detalhadas, porém separadas em processos mais complexos do menu principal: Trabalhos, Acompanhamento e Administração. Este manual, apesar de ser utilizado por vários usuários - autor, revisor, membros do CP, Administrador do sistema, Bibliotecário e Revisor de Português, terá sua linguagem direcionado para a(o) Secretária(o) e Presidente do Comitê de Publicações. Que, pela natureza de suas funções, serão a interface principal entre todos usuários e a real utilização do sistema.

NAVLivre 1.0: Procedimentos de instalação e utilização.

Este documento apresenta os procedimentos para instalação e utilização do sistema NAVLivre 1.0, um software de código livre desenvolvido para o processamento automático de imagens do sensor Advanced Very High Resolution Radiometer (AVHRR) a bordo dos satélites da National Oceanic Atmospheric Administration (NOAA). O NAVLivre é uma derivação do sistema NAVPRO, criado pela Embrapa Informática Agropecuária em parceria com a Universidade Estadual de Campinas (Unicamp), que contou com o repasse do pacote computacional NAV (NAVigation), desenvolvido pelo Colorado Center for Astrodynamics Research (CCAR), da Universidade do Colorado, Boulder, EUA. O diferencial do NAVLivre é a ausência dos módulos desenvolvidos em Interactive Data Language (IDL), presentes no NAVPRO, e dependentes de softwares proprietários. O NAVLivre é um pacote totalmente livre, que realiza de forma automática as principais etapas do processamento das imagens NOAA, como a correção radiométrica, o georreferenciamento preciso e a geração da imagem final em formato GeoTIFF, compatível com os principais pacotes de processamento de imagens. O NAVLivre é executado em plataforma Linux e foi implementado em script c-shell e linguagem C. Seu uso é indicado aos usuários avançados de imagens NOAA, que demandam o processamento em lote de grandes volumes de dados. As rotinas e scripts aqui descritos são de domínio público, podendo ser alterados conforme necessidade do usuário.

Gerenciamento de redes de computadores utilizando CACTI.

Este documento foi concebido com o intuito de ressaltar a importância do acompanhamento da disponibilidade dos recursos de uma rede de computadores. O presente trabalho consiste de um procedimento para a implementação da ferramenta CACTI, que é um software para monitoramento da disponibilidade de máquinas e serviços de uma rede de computadores, e também para a inserção dos ativos de rede que se deseja gerenciar por meio dessa ferramenta. Antes da apresentação da ferramenta CACTI, é feita uma breve explanação de alguns entre os muitos softwares de monitoramento existentes no mercado. Este trabalho destina-se a facilitar a tarefa de gerenciamento de uma rede de computadores a todos os usuários, que de alguma forma necessitem, de forma clara e objetiva, saber da disponibilidade de seus recursos, seja de forma imediata ou ao longo de um período de tempo.

Uso de imagens digitais na avaliação do crescimento da taxa de cobertura do solo.

RESUMO: Existem vários métodos para avaliar o crescimento da vegetação e a taxa de cobertura do solo. Medidas precisas e rápidas podem ser obtidas do tratamento digital de imagens geradas de câmeras fotográficas ou de vídeo. Há disponível, no mercado, diversos processadores de imagens que apresentam funções básicas semelhantes, mas com certas particularidades que poderão trazer maior benefício para o usuário, dependendo da aplicação. O SPRING, desenvolvido pelo INPE, é de domínio público, sendo mais abrangente do que um processador de imagens, incluindo funções de geoprocessamento. O ENVI foi desenvolvido para a análise de imagens multiespectrais e hiperespectrais, podendo também ser utilizado para o processamento de imagens obtidas de câmeras de vídeo, por exemplo. O KS-300 é um conjunto de hardware e de software destinado ao processamento e à quantificação de imagens microscópicas, permitindo a captação direta das imagens geradas por meio de lupas, microscópios eletrônicos ou câmeras de vídeo. O SIARCS foi desenvolvido pela Embrapa Instrumentação Agropecuária para tornar mais ágil o processo de captação de dados de um sistema. Este trabalho apresenta os fundamentos teóricos básicos envolvidos na técnica de análise de imagens, com as principais características dos softwares citados acima e sua aplicação na quantificação da taxa de crescimento e da cobertura do solo por espécies vegetais. ABSTRACT: Several methods exist to evaluate the growth of the vegetation and the tax of covering of the soil. Necessary and fast measures can be obtained of the digital treatment of generated images of photographic cameras or of video. There is available, in the market, several processors of images that you/they present similar basic functions, but with certain particularities that can bring larger benefit for the user, depending on the application. SPRING, developed by INPE, it is public domain, being including than a processor of images, including functions. ENVI was developed for the analysis of images multiespectrais and hiperespectrais, could also be used for the processing of obtained images of video cameras, for instance. The KS-300 it is a hardware group and software destined to the processing and quantification of microscopic images, allowing the direct reception of the images generated through magnifying glasses, eletronic microscopes or video cameras. SIARCS was developed by Embrapa Agricultural Instrumentation to turn more agile the process of reception of data of a system. This work presents the basic theoretical foundations involved in the technique of analysis of images, with the main characteristics of the softwares mentioned above and his application in the quantification of the growth tax and of the covering of the soil for vegetable species.

Avaliação do potencial de estabelecimento de projetos de Mecanismo de Desenvolvimento Limpo (MDL) de uso da terra, Mudança do Uso da Terra e Florestas (LULUCF) no município de Cachoeiras de Macacu, RJ - Brasil.

O aumento da concentração de Gases de Efeito Estufa (GEE) tem sido apontado como o principal agente de mudança nos processos dinâmicos da atmosfera, promovendo mudanças climáticas com ameaças à humanidade (IPCC, 2007). Em resposta aos problemas ambientais ligados às mudanças climáticas globais, a comunidade internacional adotou, em 1992, a Convenção Quadro das Nações Unidas sobre Mudança do Clima (CQNUMC) - conhecida internacionalmente pela sigla UNFCCC - United Nations Framework Convention on Climate Change. O Protocolo de Quioto, firmado em 1997, estabeleceu um acordo de metas globais coletivas para redução das emissões de gases de efeito estufa dos países industrializados a cerca de 5% dos níveis observados em 1990, entre 2008 e 2012 (UNFCCC, 1998). O Mecanismo de Desenvolvimento Limpo - MDL é o mecanismo criado no Protocolo de Quioto que permite a participação dos países em desenvolvimento na venda de Reduções Certificadas de Emissões (RCE) para os países com metas de redução de GEE.

Ataque de Lonomia sp. (Lepidoptera: Saturniidae) em seringueira no município de Bujari, AC.

Os estudos dos insetos associados às culturas agrícolas e florestais são dinâmicos, em função da evolução dos sistemas de cultivo, do avanço da fronteira agrícola, da introdução de novas pragas e da resistência aos produtos químicos historicamente aplicados. Nesse contexto, os avanços nos estudos dos insetos-praga periodicamente precisam ser integrados em publicações de referência, que permitam fácil acesso a esses conhecimentos. Assim, o objetivo desta publicação é documentar o ataque de lagartas do gênero Lonomia, popularmente conhecidas como lagartas-de-fogo, causando intensa desfolha em cultivos de seringueira (Hevea brasiliensis) no Acre. Há um grande número de trabalhos publicados sobre esse gênero, no que tange aos problemas acarretados pelo contato direto de pessoas com as lagartas, provocando acidentes hemorrágicos graves, dependendo da área lesionada e da extensão do contato. No entanto, não havia relatos dessas lagartas causando intenso desfolhamento em seringais de cultivo, ocasionando prejuízos econômicos. A partir da intensificação do cultivo, surtos populacionais de insetos, considerados pragas secundárias em muitas culturas, podem acontecer com mais frequência, necessitando de conhecimento e estratégias de manejo que visem ao monitoramento, distribuição e controle de populações de insetos no Estado do Acre, a fim de prevenir surtos populacionais de pragas e, consequentemente, prejuízos elevados aos produtores acrianos.

Farmacocinética do paracetamol

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas