EXPOSURE OF PHARMACY PERSONNEL TO ANTINEOPLASTIC DRUGS

This dissertation consists of two parts: (1) Exposure of pharmacy personnel to antineoplastic drugs. The Salmonella reversion test was used to measure the mutagenic activities of urine concentrates from individuals preparing antineoplastic drugs for intravenous administration. Longitudinal studies were performed in which the total urine produced in 24-hour periods was collected, starting on a Sunday at 7 P.M. after a duty-free weekend and extending over an eight-day period. There was no detectable increase in mutagenic activity in the urine concentrates of three pharmacy administrators who had no contact with these drugs. All six individuals admixing drugs in open-faced, horizontal laminar flow hoods displayed a two-fold increase in mutagenesis by the fourth day with peak values of 2.7 to 24-fold occurring on days five and six, reduced values by day seven with a return to the spontaneous level by day eight. When four of the six positive individuals in the preceding experiment admixed comparable amounts of antineoplastic drugs in a closed-faced, vertical laminar flow hood, no increase in mutagenic activity was detected in their urine concentrates over the eight-day period. (2) Estimate of potential carcinogenic risks of antineoplastic drugs. Excision repair is the major repair system that is involved with the elimination of chemically induced DNA (deoxyribonucleic acid) lesions. This DNA excision repair capability increases in mammalian species with longer life span such as humans. In this study, the effect of functional DNA excision repair on the mutagenesis invoked by 17 antineoplastic drugs was determined by using a Salmonella/Microsome assay which was expanded to include some uvr('+) counterparts of the excisionless (uvrB) tester strains routinely employed. Although extrapolation cannot be made from bacteria to humans, one should be able to make a qualitative comparison as to which antineoplastic drugs are more potentially carcinogenic to humans based on the effects of excision repair on their mutagenesis in bacteria. The tested antineoplastic drugs were divided into three classes: those requiring excision repair for mutagenesis; those producing nonrepairable genetic damage; and those producing mostly repairable premutational DNA lesions. ^

Paisaje : Imagen, entorno, ensamble

Aun reconociendo su situación mediadora entre cultura y naturaleza, tanto las disciplinas estéticas como las ciencias sociales suelen reducir el paisaje, o bien a una mera construcción de la mirada que lo abarca o bien al resultado material que la intervención humana deja al "culturizar" su entorno natural. Este ensayo argumenta que, en cambio, tenemos que volver a pensar al paisaje como el proceso que va de la imagen al entorno y viceversa, ensamblando lo humano con lo no-humano. De esta manera, se propone esbozar un marco teórico para historizar el paisaje en la modernidad latinoamericana.

Paisaje : Imagen, entorno, ensamble

Aun reconociendo su situación mediadora entre cultura y naturaleza, tanto las disciplinas estéticas como las ciencias sociales suelen reducir el paisaje, o bien a una mera construcción de la mirada que lo abarca o bien al resultado material que la intervención humana deja al "culturizar" su entorno natural. Este ensayo argumenta que, en cambio, tenemos que volver a pensar al paisaje como el proceso que va de la imagen al entorno y viceversa, ensamblando lo humano con lo no-humano. De esta manera, se propone esbozar un marco teórico para historizar el paisaje en la modernidad latinoamericana.

Paisaje : Imagen, entorno, ensamble

Aun reconociendo su situación mediadora entre cultura y naturaleza, tanto las disciplinas estéticas como las ciencias sociales suelen reducir el paisaje, o bien a una mera construcción de la mirada que lo abarca o bien al resultado material que la intervención humana deja al "culturizar" su entorno natural. Este ensayo argumenta que, en cambio, tenemos que volver a pensar al paisaje como el proceso que va de la imagen al entorno y viceversa, ensamblando lo humano con lo no-humano. De esta manera, se propone esbozar un marco teórico para historizar el paisaje en la modernidad latinoamericana.

Rare earth elements from the Atlantic Sector

We present a Rare Earth Elements (REE) record at decadal resolution determined in the EPICA ice core drilled in Dronning Maud Land (EDML) in the Atlantic Sector of the East Antarctic Plateau, covering the transition from the last glacial age (LGA) to the early Holocene (26 600-7500 yr BP). Additionally, samples from potential source areas (PSAs) for Antarctic dust were analysed for their REE characteristics. The dust provenance is discussed by comparing the REE fingerprints in the ice core and the PSAs samples. We find a shift in REE composition at 15 200 yr BP in the ice core samples. Before 15 200 yr BP, the dust composition is very uniform and its provenance was likely to be dominated by a South American source. After 15 200 yr BP, multiple sources such as Australia and New Zealand become relatively more important, albeit South America is possibly still an important dust supplier. A similar change in the dust characteristics was observed in the EPICA Dome C ice core at around ~15 000 yr BP. A return to more glacial dust characteristics between ~8300 and ~7500 yr BP, as observed in the EPICA Dome C core, could not be observed in the EDML core. Consequently, the dust provenance at the two sites must have been different at that time.

Returns to migration : the role of educational attainment in rural Tanzania

Given the migration premium previously identified in an impact evaluation approach, this paper asks the question of why migration is not more prominent, given such high premium associated with it. Using long-term household panel data drawn from rural Tanzania, Kagera for the period 1991-2004, this study aims to answer this question by exploring the contribution of education in the migration premium. By separating migrants into those that moved out of original villages but remained within Kagera and those who left the region, this study finds that, in consumption, the return on investment in education is higher at both destinations. However, whilst the higher return on education fully explains the gains associated with migration within Kagera, it only partly explains those of external migration. These findings suggest that welfare opportunities are higher at the destination and that an individual's limited investment in education plays a major role in preventing short-distance migration from becoming a significant source of raising welfare, which is not the case for long-distance migration. While education plays a role, it appears that other mechanisms may prohibit rural agents from exploiting the arbitrage opportunity when they migrate to the destination at a great distance from the source.

Optimization of urban mobility measures to achieve win-win strategies

European cities are essential in the development of Europe as they constitute the living environment of more than 60% of the population in the European Union and are drivers of the European economy – just under 85% of the EU’s gross domestic product is produced in urban areas (EC, 2007a). The car has been one of the main factors of development during the 20th century, but it is at the same time the origin of the key problems cities have to face: traffic increase. This has resulted in chronic congestion with many adverse consequences such as air pollution and noise. This loss of environmental quality is one of the reasons for urban sprawl in European cities during recent decades. But this urban sprawl at the same time worsens the environmental conditions. We must return to the dense city, but clean and competitive, and this implies reducing car use yet provides quality transport alternatives sufficient to recover and maintain the competitiveness of cities (EC, 2007a). Consequently, European cities need to establish an urban transport strategy which helps reduce their environmental problems –mainly emissions and noise – but without decreasing their trip attraction. This aspect is very important because a loss of trip attraction would result in an increase of people moving to more disperse areas, contributing towards worsening the current situation. This thesis is an attempt to contribute solutions to this problem in two ways: 1) The first is to analyze the complementarity and possible synergies of several urban transport measures aimed at improving a modal split to a more sustainable means of transport. This analysis will focus on the three aspects already mentioned: emissions, noise and attractiveness or competitiveness. 2) Once possible synergies and complementarities have been analyzed, the second objective is to propose the best combination of these measures, in terms of level of implementation, to achieve the maximum benefit with respect to the three aspects previously established: emissions, noise and attractiveness or competitiveness. Therefore, within the wide range of measures enhancing sustainable urban transport, three of them have been be selected in this thesis to establish a methodology for achieving these objectives. The analysis will be based on the region of Madrid, which is also the case study selected for this research. Las ciudades europeas son piezas fundamentales para el desarrollo europeo, ya que son el lugar de residencia de más del 60% de la población de la unión europea así como los motores de su economía – casi el 85% del PIB europeo se produce en áreas urbanas (EC, 2007a). El coche ha sido uno de los principales motores de desarrollo de las ciudades durante el siglo XX, pero se ha terminado por convertir a su vez en uno de los principales problemas con los que tiene que lidiar las ciudades: el aumento del tráfico. Esto ha derivado en unos niveles crónicos de congestión, con multitud de efectos adversos, entre los que cabe destacar la contaminación del aire y el ruido. Esta pérdida de calidad ambiental es una de las razones que ha propiciado la dispersión urbana que han experimentado las ciudades europeas en las últimas décadas. Pero esta dispersión urbana a su vez contribuye a empeorar las condiciones ambientales de las ciudades. Debemos retornar a la ciudad densa, pero limpia y competitiva, y esto implica reducir el uso del coche, pero proporcionando alternativas de transporte que permitan recuperar y mantener la competitividad de las ciudades (EC, 2007a). Por lo tanto, las ciudades europeas necesitan encontrar una estrategia de transporte urbano que ayude a reducir sus problemas medio ambientales – principalmente ruido y emisiones – pero sin hacerlas perder atractividad o competitividad. Este aspecto tiene gran importancia porque una pérdida de la misma se traduciría en un aumento de dispersión de la población hacia áreas periféricas, contribuyendo a empeorar la situación actual. Esta tesis contribuye a solucionar este problema de dos maneras: 1) La primera, analizando la complementariedad y posibles sinergias de diferentes medidas de transporte urbano orientadas a promover un reparto modal hacia modos más sostenibles. Este análisis se centrará en los tres aspectos anteriormente citados: emisiones, ruido y atractividad o competitividad. 2) Una vez las posibles sinergias y complementariedades se han analizado, el segundo objetivo es proponer la mejor combinación de estas medidas – en términos de grado de aplicación - para lograr el máximo beneficio en lo que respecta a los tres objetivos previamente establecidos. Para ello, en esta tesis se han seleccionado una serie de medidas que permitan establecer una metodología para alcanzar estos objetivos previamente definidos. El análisis se centra en la ciudad de Madrid y su área metropolitana, la cual se ha escogido como caso de estudio para realizar esta investigación.

Cadena de valor de papas nativas (Solanum andigenum sp.) en la provincia de Jauja, Perú

La Asamblea General de la ONU, a solicitud del gobierno peruano, declara en el año 2008 el Año Internacional de la Papa, (AIP). Desde el año 2005, el gobierno peruano ha puesto en marcha estrategias en torno a la importancia de la papa, como la declaración del 30 de Mayo como el Día Nacional de la Papa. El año 2014 es declarado por la FAO, (Food and Agriculture Organization of the United Nations), Año Internacional de la Agricultura Familiar, con un enfoque compartido por esta Tesis Doctoral en el apoyo a la familia campesina peruana. El gobierno peruano utiliza las cadenas de valor como una estrategia de promoción de desarrollo sostenible, que ha permitido contribuir a la inclusión social y económica de productores pobres de zonas alto andinas, como las localizadas en la mancomunidad municipal del Yacus, (MMY), provincia de Jauja, departamento de Junín. Esta estrategia, en la que el mercado incorpora a los pequeños agricultores de las zonas altas de los Andes, (que disponen de recursos económicos muy bajos), en procesos productivos rentables, implica una serie de cambios a realizar, como la transformación de los patrones de producción tradicional hacia aquellos productos o servicios que tienen demanda en el mercado, o la variación de la mentalidad del agricultor pequeño hacia una concepción empresarial de su producción. (Fabián, 2013). Por otra parte, la sostenibilidad de las cadenas de valor depende del eslabón más débil, lo que obliga a conocer la situación de todos los eslabones para poder integrar y reforzar la cadena. Se requiere un sistema de transparencia adecuado que facilite el flujo de la información entre los distintos eslabones. (Briz et al., 2012). Además, el establecimiento de la cadena de valor debe hacerse con cuidado, ya que la eficacia y supervivencia de las empresas están cada vez más ligadas a la cadena de valor a la que pertenece y a la coordinación de la misma. (Briz, 2011). En esta situación, adquiere importancia el estudio de la cadena de valor de la papa nativa, para una vez establecidas sus características, poder determinar la viabilidad o no de una cadena de valor de este producto que repercuta parte de la riqueza generada en los pequeños agricultores alto andinos, e incluso, de su extensión hasta España. Existen estudios sobre las papas nativas, realizadas por diferentes universidades de distintos países, e incluso, de diferentes continentes. Sin embargo, la mayoría de los estudios se centran en la mejora de la producción de la papa nativa. La novedad de la investigación realizada en la presente Tesis Doctoral radica en el estudio de la viabilidad de la comercialización de la papa nativa, mediante el establecimiento de una cadena de valor que se inicie en la provincia de Jauja, Perú, y finalice tanto en los mercados peruanos de las regiones de Junín y Lima, como en España. El objetivo planteado en esta investigación es la mejora de las condiciones económicas y sociales de las comunidades agrícolas de la provincia de Jauja en el Perú, así como fomentar su desarrollo tecnológico e industrial, mediante el fomento de la cadena de valor de la papa nativa y sus derivados. Se establecen como objetivos específicos la caracterización de los eslabones de la cadena de valor de la papa nativa y sus derivados en la provincia de Jauja del Perú y en España, de manera que se determine el valor agregado en los mismos; el fortalecimiento de las organizaciones de productores de papas nativas para la comercialización de sus producciones y para el fomento de la cultura empresarial; y el desarrollo de una cadena de comercialización papas nativas y sus derivados con origen en la Provincia de Jauja, Perú, y que finalice en España, con la venta al consumidor español. Para alcanzar estos objetivos la metodología utilizada es la cadena de valor agroalimentaria, utilizando como herramientas de análisis el análisis DAFO de la cadena de valor de la papa nativa. Las fuentes de información primarias utilizadas proceden en parte del proyecto de cooperación de UPM, “Mejora de la cadena de valor de la patata andina como impulso al desarrollo rural. Caso de tres Comunidades Campesinas en la Provincia de Jauja del Perú”, en el que participó el doctorando, y en parte proceden de la batería de encuestas específicamente diseñadas para los diferentes eslabones de la cadena de valor de la papa nativa. Las fuentes de información secundarias proceden de artículos académicos publicados, de artículos publicados por revistas especializadas del sector y de informes realizados por diferentes instituciones gubernamentales, tanto españolas como peruanas. Las conclusiones de la investigación son las siguientes. La creación de la mancomunidad del Yacus ha beneficiado a los pequeños agricultores. Estos consiguen mejores condiciones de venta y mejores precios para sus productos, lo que repercute en la mejora de sus condiciones de vida. Estas mejoras en las condiciones de venta de los productos se deben a su pertenencia a una cadena de valor de papa nativa que está funcionando de forma eficaz. Las empresas consideradas para constituir la cadena de valor han mostrado interés por formar parte de ella: los campesinos para obtener mejores precios por sus productos y unas mejores condiciones de venta; los distribuidores para asegurarse una calidad determinada de unas variedades fijas de papa nativa; la industria transformadora por disponer de un suministro de producto adecuado al derivado de papa nativa correspondiente, (hojuelas, tunta, etc.); las empresas exportadoras para tener suministro garantizado de los productos que ellos requieren en los volúmenes adecuados. Es una situación ventajosa para todas las empresas participantes. A pesar de trabajar con un producto tradicional, la cadena de valor de la papa nativa presenta innovación en los productos comercializados, tanto en la papa nativa fresca como en sus derivados, en los formatos de los productos, en la red de distribución, en las instituciones peruanas y en el consumidor final. Se percibe una demanda de papa nativa y de sus productos derivados en aquellos países donde existen comunidades de latinoamericanos que han emigrado de sus países de origen. España está entre los países que han acogido a un importante número de personas de origen latinoamericano. A pesar de la fuerte crisis económica sufrida por España, que ha llevado consigo la vuelta a sus países de origen de parte de su comunidad latinoamericana, el tamaño de esta población sigue siendo importante. Esta población demanda productos originarios de sus propios países, y los consumirían de forma frecuente si los precios son adecuados a su capacidad de consumo. El precio de venta de la papa nativa y sus derivados en España es de gran importancia. La importación de estos productos desde Perú hace que este eleve a niveles que le resta competitividad, en especial en la papa fresca. Se aconseja la búsqueda de empresas que puedan adaptar la producción de la papa fresca de forma local, y mantener para los derivados la exportación directa a España. Las preferencias de los consumidores peruanos y españoles en cuanto a formatos y marcas se refieren no son coincidentes. De las encuestas realizadas, se concluye que no puede seguirse la misma estrategia de marketing en ambos países, debiéndose diferenciar los formatos de los paquetes de la papa nativa y de sus derivados en España y en Perú, para así lograr llegar a los consumidores potenciales de ambos países. ABSTRACT At the request of the Peruvian government, the UN General Assembly declared the International Year of the Potato in 2008. Since 2005, the Peruvian government has implemented strategies around the importance of the potato, as the declaration of the 30th of May as the National Day of the Potato. FAO (Food and Agriculture Organization of the United Nations) has declared 2014 as the International Year of Family Farming, with an approach shared by this Ph.D. dissertation about the Peruvian peasant family. The Peruvian government uses value chains as a strategy to promote sustainable development, which has allowed to contribute to the social and economic inclusion of poor farmers in the high Andean regions as those located in the municipal commonwealth of Yacus (MMY) province of Jauja, department of Junín. This strategy, which incorporates small farmers in the high Andean regions, (who have very low income), to the market with profitable production processes, implies a number of changes that should take place, such as changing patterns of traditional production to those products or services that are in demand in the market, or changes in the mentality of the small farmer into a concept of production business. (Fabián, 2013). Moreover, the sustainability of value chains depends on the weakest link, which demands a knowledge of the status of all the links, in order to integrate and strengthen the chain. It is required an adequate transparency to facilitate the flow of information between the various actors. (Briz et al., 2012). Furthermore, the establishment of the value chain should be done carefully, since the effectiveness and the survival of the businesses are increasingly linked to the value chain where the firm is included and to its coordination. (Briz, 2011). In this situation, it becomes important to study the value chain of the native potato, once we establish its features, to be able to determine the feasibility or not of a value chain of this product, which has an impact of the generated wealth in small farms of the high Andean regions, and even the extension of this value chain to Spain. There are studies on native potatoes, made by different universities in several countries and even in more than one continent. However, most studies focus on improving the production of native potato. The originality of the research conducted in this Ph.D. dissertation is the study of the feasibility of commercialization of native potato, by the creation of a value chain that starts in the province of Jauja, Perú, and ends both in Peruvian markets in the region of Lima, and in Spain. The main goal of this research is to improve the economic and social conditions of farming communities in the province of Jauja in Perú, while promoting its technological and industrial development, by the establishment of a value chain of the native potato and derivatives. The specific objectives of the research are the characterization of the links in the value chain of the native potato and its derivatives in the province of Jauja, (Perú) and in Spain, in order to determine the added value; the strengthening of organizations of native potato producers, to commercialize their products and the promotion of enterprise culture; and the development of a chain to market native potato and its derivatives, with its origin in the province of Jauja, (Perú), and its end in Spain, with the sale to the Spanish consumer. In order to achieve these objectives, the used methodology is the agrifood value chain, using as a tool to analysis it the SWOT analysis of the value chain of the native potato. The primary sources of information used in the research come partly from UPM cooperation project, "Improving the value chain of Andean potato as a boost to rural development. Case Three Rural Communities in the Province of Jauja, (Perú)", in which the Ph.D. student was involved, and partly from the surveys, which were specifically designed for the different links of the value chain of the native potato. The secondary sources of information come from academic articles, from articles published by magazines of the industry, and from reports of several government institutions, both Spanish and Peruvian. The conclusions of the research are as follows. The creation of the commonwealth of Yacus has benefited small farmers. They get better sales conditions and better prices for their products, which results in the improvement of their living conditions. These improvements are due to a value chain of native potato which is working effectively. All the firms invited to constitute the value chain have shown interest in being part of it: the farmers to get better prices for their products and better sale conditions; the distributors to ensure a certain quality of fixed varieties of native potato, the processing industry in order to have an adequate supply of product to the corresponding derivative of native potato (chips, “tunta”, etc.); exporting firms to have a guaranteed supply of the products that they require with the right volumes. It's a win-win situation for all participating companies. Despite being a traditional product, the value chain of the native potato presents innovation in marketed products, (both fresh native potato and its derivatives), in the formats of products, in the distribution network, in Peruvian institutions and in relation with the consumer. There is a perceived demand of native potato and its products in countries where communities of Latin Americans have settled down. Spain is among the countries that have received a significant number of people from Latin America. Despite the strong economic crisis suffered by Spain, which has lead to a return to their home countries of part of the Latin American community, the size of this population is still considerable. This population demands products from their own countries, and they frequently consume them if the prices are suitable to their standard of living. The selling price of the native potato and its derivatives in Spain is of great importance. The import of these products from Perú makes the prices rise to levels that reduce competitiveness, especially in fresh native potatoes. It is advised to look for companies which can adapt the fresh potato production in our country, and keep direct export to Spain for the derivatives products. The preferences of Peruvian and Spanish consumers in terms of formats and brands are not the same. The surveys concluded that the same marketing strategy cannot be followed in both countries. Packet formats of native potato and its derivatives should be differentiated in Spain and Perú, in order to reach the potential consumers of both countries.

Estudio de los parámetros necesarios para establecer las medidas de actuación por tipos de buques en situaciones de emergencia después de un accidente

La “Actuación en la Crisis”, objetivo principal de esta tesis, trata de establecer y concretar los procedimientos y apoyos desde tierra y a bordo de los buques, tanto técnicos como operacionales, a seguir por el Capitán y tripulación de un buque después de un accidente, en especial cuando el buque tiene un riesgo importante de hundimiento o necesidad de abandono. La aparición de este concepto es relativamente reciente, es decir desde el año 1995, después de los estudios y propuestas realizados, por el Panel de Expertos de IMO, como consecuencia del hundimiento del buque de pasaje y carga rodada, Estonia, en el que perdieron la vida más de 850 personas a finales de Septiembre de 1994. Entre las propuestas recomendadas y aceptadas por los gobiernos en la Conferencia Internacional SOLAS 1995, figuraba este concepto novedoso, que luego fue adoptado de una forma generalizada para todos los tipos de buques, que hasta entonces sólo disponían de documentos dispersos y a veces contradictorios para la actuación en estos momentos de peligro, que dio lugar a un profundo tratamiento de este problema, que iba a afectar a los buques, tanto en los conceptos y parámetros de proyecto, como a la propia operación del buque. La tesis desarrolla los fundamentos, estado del arte, implantación y consecuencias sobre la configuración y explotación del buque, que han dado lugar a una serie de documentos, que se han incluido en diversos Convenios Internacionales, Códigos y otros documentos de obligada aplicación en la industria naval generados en IMO (SOLAS, Retorno Seguro a Puerto, Plano y Libro de Control de Averías, ISM). La consecuencia más novedosa e interesante de este concepto ha sido la necesidad de disponer cada compañía explotadora del buque, de un servicio importante de “apoyo en la crisis”, que ha dado lugar a implantar un “servicio de emergencia especial”, disponible las 24 horas del día y 365 de año que ofrecen las Sociedades de Clasificación. El know-how de los accidentes que tratan estos servicios, hacen que se puedan establecer ciertas recomendaciones, que se centran, en que el buque tenga, por sus propios medios, una posibilidad de aumentar el KM después de una avería, la garantía de la resistencia estructural adecuada y el aumento del tiempo de hundimiento o el tiempo de mantenimiento a flote (otro tipo de averías vinculadas con la maquinaria, equipo o protección y lucha contra incendios, no son objeto de tesis). Las conclusiones obtenidas, son objeto de discusión especialmente en IACS e IMO, con el fin de establecer las aplicaciones pertinentes, que permitan dar al buque una mayor seguridad. Como objetivo principal de esta tesis es establecer estos puntos de mejora consecuencia de esta actuación en la crisis, con la aportación de varias soluciones que mejorarían los problemas mencionados para los tres tipos de buques que consideramos más importantes (pasaje, petroleros y bulkcarriers) La tesis recorre, desde el principio en 1995, la evolución de esta actuación en la crisis, hasta el momento actual., los puntos básicos que se establecen, que van muy de la mano de la llamada “cultura de seguridad”, objetivo nacido durante los años 90, con el fin de implantar una filosofía distinta para abordar el tratamiento de la seguridad del buque, a la que se venía aplicando hasta el momento, en donde se contemplaba tratar el tema de forma singular y específica para cada caso. La nueva filosofía, trataba de analizar el problema, desde un aspecto global y por tanto horizontal, realizando un estudio exhaustivo de las consecuencias que tendría la aplicación de una nueva medida correctora, en los restantes equipos y sistemas del buque., relativos al proyecto, configuración, operación y explotación del buque. Se describen de manera sucinta las profundas investigaciones a que dio lugar todo lo anterior, estando muchas de ellas, vinculadas a grandes proyectos europeos. La mayor parte de estos proyectos fueron subvencionados por la Comunidad Económica Europea durante la primera década del siglo actual. Dentro de estas investigaciones, donde hay que destacar la participación de todos los agentes del sector marítimo europeo, se hacen imprescindibles la utilización de dos herramientas novedosas para nuestro sector, como son el “Estudio de Riesgos” y la “Evaluación de la Seguridad”, más conocida técnicamente por su nombre ingles “Safety Assessment”, cuyos principios también son incluidos en la tesis. Además se especifican las bases sobre las que se establecen la estabilidad intacta y en averías, con nuevos conceptos, no tratados nunca hasta entonces, como la “altura crítica de agua en cubierta” para la cual el buque se hundiría sin remisión, “estado de la mar” en la que se puede encontrar el buque averiado, el cálculo del tiempo de hundimiento, u otros aspectos como el corrimiento de la carga, o bien el tratamiento de los problemas dinámicos en el nuevo “Código de Estabilidad Intacta”. Con respecto a la resistencia estructural, especialmente el estudio de la “resistencia estructural después de la avería”, que tiene en cuenta el estado de la mar en la que se encontraría el buque afectado. Se analizan los tipos de buques mencionados uno por uno y se sacan, como aportación fundamental de esta tesis, separadamente, las acciones y propuestas a aplicar a estos buques. En primer lugar, las relativas al proyecto y configuración del buque y en segundo lugar, las de operación, explotación y mantenimiento, con el fin de acometer, con garantías de éxito, la respuesta a la ayuda en emergencia y la solución a la difícil situación que pueden tener lugar en condiciones extremas. Para ver el efecto de algunas de las propuestas que se incluyen, se realizan y aplican concretamente, a un buque de pasaje de carga rodada, a un petrolero y a un bulkcarrier, para demostrar el mejor comportamiento de estos buques en situación de emergencia. Para ello se han elegido un buque ejemplo para cada tipo, efectuándose los cálculos de estabilidad y resistencia longitudinal y comparar la situación, en la que quedaría el buque averiado, antes y después de la avería. La tesis se completa con una estadística real de buques averiados de cada uno de estos tres tipos, distinguiendo el tipo de incidente y el número de los buques que lo han sufrido, considerándose como más importantes los incidentes relacionados con varadas, colisiones y fuego resumiéndose lo más relevante de esta aportación también importante de esta tesis. ABSTRACT The "Response in an emergency" is the main objective of this thesis, it seeks to establish and define procedures for technical and operational support onboard and shore, to be followed by the captain and crew on of a ship after an accident, especially when the ship has a significant risk of sinking or a need to abandon it. The emergence of this concept is relatively recent, in 1995, after studies and proposals made by the Panel of Experts IMO, following the sinking of the “Estonia” vessel, where more than 850 people died in late September 1994. In the International Convention SOLAS 1995, among the recommended proposals and accepted regulations, this new concept was included, which was later adopted for all types of ships which until then had only scattered some documents, sometimes including contradictory actions in emergency situations. This led to a profound treatment of this problem, which would affect the vessels in both the concepts and design parameters, as to the proper operation of the vessel. The thesis develops the foundations, state of the art, implementation and consequences on the design and operation of the vessel, this has led to a series of Circulars and Regulations included in several International Codes and Conventions issued by IMO which are required to be complied with (SOLAS Safe Return to Port, Damage Control Plan and Booklet, ISM). The most novel and interesting consequence of this concept has been the need for every company operating the ship to have a shore based support service in emergency situations which has led to implement special emergency services offered by Class Societies which are available 24 hours a day, 365 days per year. The know-how of these services dealing with all types of accidents can establish certain recommendations, which focus on the ship capability to increase the KM after damage. It can also be determined adequate structural strength and the increase of the capsizing time or time afloat (other types of damages associated with the machinery, equipment or firefighting, are not the subject of this thesis). The conclusions are discussed especially in IACS and IMO, in order to establish appropriate applications to improve the security of the vessels. The main objective of this thesis is to establish actions to improve emergency actions, resulting from different responses in the crisis, with the contribution of several solutions that improve the problems mentioned for three types of ships that we consider most important (passenger vessels, tankers and bulk carriers) The thesis runs from the beginning in 1995 to date, the evolution of the response on the crisis. The basics established during the 90s with the "safety culture" in order to implement a different philosophy to address the treatment of the safety of the ship, which was being previously implemented, as something singular and specific to each case. The new philosophy tried to analyse the problem from a global perspective, doing an exhaustive study of the consequences of the implementation of the new regulation in the ship systems and equipment related to the design, configuration and operation of the vessel. Extensive investigations which led to the above are described, many of them being linked to major European projects. Most of these projects were funded by the European Union during the first decade of this century. Within these investigations, which it must be highlighted the participation of all players in the European maritime sector, a necessity to use two new tools for our industry, such as the "Risk Assessment" and "Safety Assessment" whose principles are also included in the thesis. The intact and damage stability principles are established including new concepts, never treated before, as the "critical height of water on deck" for which the ship would sink without remission, "sea state" where the damaged vessel can be found, calculation of capsizing time, or other aspects such cargo shifting or treatment of dynamic problems in the new Intact Stability Code in development. Regarding the structural strength, it has to be especially considered the study of the "residual strength after damage", which takes into account the state of the sea where the vessel damaged can be found. Ship types mentioned are analysed one by one, as a fundamental contribution of this thesis, different actions and proposals are established to apply to these types of vessels. First, those ones relating to the design and configuration of the vessel and also the ones related to the operation and maintenance in order to support successfully responses to emergency situations which may occur in extreme situations. Some of the proposals are applied specifically to a RoRo passenger ship, an oil tanker and a bulkcarrier, to demonstrate the improved performance of these vessels damaged. An example for each type vessel has been chosen, carrying out stability and longitudinal strength calculations comparing the situation of the ship before and after damage. The thesis is completed with incidents statics for each of these three types, distinguishing the type of incident and the number of ships having it. The most important incidents considered are the ones related to groundings, collisions and fire being this other relevant contribution of this thesis.

Respiratory microbiota resistance and resilience to pulmonary exacerbation and subsequent antimicrobial intervention

This study was supported by the UK Natural Environment Research Council (NE/H019456/1) to CJvdG, by the Wellcome Trust (WT 098051) to AWW and JP for sequencing costs, and by The Anna Trust (KB2008) to KDB. AWW and The Rowett Institute of Nutrition and Health, University of Aberdeen, receive core funding support from the Scottish Government Rural and Environmental Science and Analysis Service (RESAS). We thank Paul Scott, Richard Rance and the Wellcome Trust Sanger Institute’s sequencing team for generating 16S rRNA gene sequence data.

Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress

Erythropoietin (EPO) promotes neuronal survival after hypoxia and other metabolic insults by largely unknown mechanisms. Apoptosis and necrosis have been proposed as mechanisms of cellular demise, and either could be the target of actions of EPO. This study evaluates whether antiapoptotic mechanisms can account for the neuroprotective actions of EPO. Systemic administration of EPO (5,000 units/kg of body weight, i.p.) after middle-cerebral artery occlusion in rats dramatically reduces the volume of infarction 24 h later, in concert with an almost complete reduction in the number of terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling of neurons within the ischemic penumbra. In both pure and mixed neuronal cultures, EPO (0.1–10 units/ml) also inhibits apoptosis induced by serum deprivation or kainic acid exposure. Protection requires pretreatment, consistent with the induction of a gene expression program, and is sustained for 3 days without the continued presence of EPO. EPO (0.3 units/ml) also protects hippocampal neurons against hypoxia-induced neuronal death through activation of extracellular signal-regulated kinases and protein kinase Akt-1/protein kinase B. The action of EPO is not limited to directly promoting cell survival, as EPO is trophic but not mitogenic in cultured neuronal cells. These data suggest that inhibition of neuronal apoptosis underlies short latency protective effects of EPO after cerebral ischemia and other brain injuries. The neurotrophic actions suggest there may be longer-latency effects as well. Evaluation of EPO, a compound established as clinically safe, as neuroprotective therapy in acute brain injury is further supported.

Does a neuroimmune interaction contribute to the genesis of painful peripheral neuropathies?

Painful peripheral neuropathies are precipitated by nerve injury from disease or trauma. All such injuries will be accompanied by an inflammatory reaction, a neuritis, that will mobilize the immune system. The role of the inflammation itself is difficult to determine in the presence of structural damage to the nerve. A method has been devised to produce a focal neuritis in the rat sciatic nerve that involves no more than trivial structural damage to the nerve. This experimental focal neuritis produces neuropathic pain sensations (heat- and mechano-hyperalgesia, and cold- and mechano-allodynia) in the ipsilateral hind paw. The abnormal pain sensations begin in 1–2 days and last for 4–6 days, with a subsequent return to normal. These results suggest that there is a neuroimmune interaction that occurs at the outset of nerve injury (and perhaps episodically over time in slow developing conditions like diabetic neuropathy) that produces neuropathic pain. The short duration of the phenomena suggest that they may prime the system for more slowly developing mechanisms of abnormal pain (e.g., ectopic discharge in axotomized primary afferent neurons) that underlie the chronic phase of painful neuropathy.

Direct conversion of an oligopeptide from a β-sheet to an α-helix: A model for amyloid formation

A 16-amino acid oligopeptide forms a stable β-sheet structure in water. In physiological solutions it is able to self-assemble to form a macroscopic matrix that stains with Congo red. On raising the temperature of the aqueous solution above 70°C, an abrupt structural transition occurs in the CD spectra from a β-sheet to a stable α-helix without a detectable random-coil intermediate. With cooling, it retained the α-helical form and took several weeks at room temperature to partially return to the β-sheet form. Slow formation of the stable β-sheet structure thus shows kinetic irreversibility. Such a formation of very stable β-sheet structures is found in the amyloid of a number of neurological diseases. This oligopeptide could be a model system for studying the protein conformational changes that occurs in scrapie or Alzheimer disease. The abrupt and direct conversion from a β-sheet to an α-helix may also be found in other processes, such as protein folding and protein–protein interaction. Furthermore, such drastic structure changes may also be exploited in biomaterials designed as sensors to detect environmental changes.

Significados da reinserção escolar de crianças após queimaduras: um estudo etnográfico

As queimaduras na infância compreendem importante causa de atendimento hospitalar e internação e podem desencadear sequelas físicas e emocionais. Dependendo da gravidade e complexidade das lesões, a hospitalização da criança é prolongada para realização de tratamento adequado e ela é afastada tanto do convívio familiar como do social. O número de crianças em idade escolar que sofrem queimaduras, e consequentemente interrompem as atividades escolares, por períodos curtos ou até as abandonam, é significativo, tornando digno de preocupação o processo de reinserção escolar. Este estudo teve como objetivo interpretar os significados do processo de reinserção escolar de crianças sobreviventes de queimaduras. Realizou-se uma pesquisa com abordagem metodológica qualitativa, fundamentada no referencial teórico da Antropologia Interpretativa e no método etnográfico. Após a aprovação do estudo pelos Comitês de Ética em Pesquisa com Seres Humanos das instituições envolvidas, convidaram-se crianças sobreviventes de queimaduras em seguimento em um hospital-escola do interior paulista. Coletaram-se dados no hospital, no domicílio e na escola das crianças, de janeiro de 2012 a dezembro de 2013, por meio de entrevistas em profundidade audiogravadas e observação participante, complementadas pelo diário de campo. Participaram da pesquisa 14 crianças e os atores sociais envolvidos neste processo, como familiares, profissionais de saúde, professores, vizinha e amigo, totalizando 57 participantes. A coleta e análise dos dados ocorreram concomitantemente, e esta última seguiu os pressupostos da análise temática indutiva. Identificaram-se códigos, os quais, posteriormente, embasaram a construção das duas unidades de sentidos: \"Fatos e fatores prévios ao retorno escolar\" e \"A volta à escola\". A partir destas, identificou-se o núcleo temático, \"O olho puxa essas coisas. A gente olha mesmo\": enxergando o outro como diferente, o qual apresenta a explicação compreensiva do processo de reinserção escolar, fornecendo os significados da experiência. Os significados foram explicados por meio dos conceitos antropológicos de estigma, identidade e corporeidade. O culto ao corpo presente no contexto cultural brasileiro foi fator intensificador do estigma sofrido pelas crianças. Identificaram-se fatores facilitadores do processo, como: dar continuidade às atividades escolares e manter contato com professores e colegas durante a hospitalização; preparar a escola para receber a criança; abordar sobre queimadura na escola para evitar curiosidade; encarar a presença da discriminação e trabalhar as diferenças. Estas estratégias fazem com que todos os envolvidos se sintam ao menos mais confortáveis durante esse difícil processo que merece atenção e envolvimento dos familiares, profissionais de saúde e equipe escolar. A partir dos resultados desta pesquisa, esperamos empoderar as crianças e seus familiares acerca da melhor maneira para lidar com as queimaduras durante a reinserção escolar e contribuir para o desenvolvimento de ações e estratégias baseadas na cultura para que este processo ocorra da forma menos traumática possível

Slim Styles and the Brothers of the Light

Slim Styles and the Brothers of the Light consists of a short novella, intended to be part of a series, introduced by a narrative essay. The work is about the search for identity, the pursuit of happiness, and the struggle to maintain self assurance while finding a place in the world. The story follows the main character as he slowly learns the truth about an organization he joined looking for a sense of value and worth. With his back against the wall he has to return to the home he was taught to be ashamed of, face the friends he left behind, and apologize to the lover he took for granted. The manuscript is a work in progress.