I. Studies on alkaline phosphatase activity in developing sea urchin embryos. II. Studies on the activation of protein biosynthesis in sea urchin eggs at fertilization

I. Alkaline phosphatase activity in the developing sea urchin Lytechinus pictus has been investigated with respect to intensity at various stages, ionic requirements and intracellular localization. The activity per embryo remains the same in the unfertilized egg, fertilized egg and cleavage stages. At a time just prior to gastrulation (about 10 hours after fertilization) the activity per embryo begins to rise and increases after 300 times over the activity in the cleavage stages during the next 60 hours.

The optimum ionic strength for enzymatic activity shows a wide peak at 0.6 to 1.0. Calcium and magnesium show an additional optimum at a concentration in the range of 0.02 to 0.07 molar. EDTA at concentrations of 0.0001 molar and higher shows a definite inhibition of activity.

The intracellular localization of alkaline phosphatase in homogenates of 72-hour embryos has been studied employing the differential centrifugation method. The major portion of the total activity in these homogenates was found in mitochondrial and microsomal fractions with less than 5% in the nuclear fraction and less than 2% in the final supernatant. The activity could be released from all fractions by treatment with sodium deoxycholate.

II. The activation of protein biosynthesis at fertilization in eggs of the sea urchins Lytechinus pictus and Strongylocentrotus purpuratus has been studied in both intact eggs and cell-free homogenates. It is shown that homogenates from both unfertilized and fertilized eggs are dependent on potassium and magnesium ions for optimum amino acid incorporation activity and in the case of the latter the concentration range is quite narrow. Though the optimum magnesium concentrations appear to differ slightly in homogenates of unfertilized and fertilized eggs, in no case was it observed that unfertilized egg homogenates were stimulated to incorporate at a level comparable to that of the fertilized eggs.

An activation of amino acid incorporation into protein has also been shown to occur in parthenogenetically activated non-nucleate sea urchin egg fragments or homogenates thereof. This activation resembles that in the fertilized whole egg or fragment both in amount and pattern of activation. Furthermore, it is shown that polyribosomes form in these non-nucleate fragments upon artificial activation. These findings are discussed along with possible mechanisms for activation of the system at fertilization.

Caretta caretta (Loggerhead Sea Turtle). Nest Architecture.

Researchers compared nest architecture in loggerhead sea turtles at natural beaches in Florida, USA and Brazil to determine how similarities and differences in female morphology and reproductive output in these two populations are reflected in the structure of the nest.

New measurements on the absolute cosmic ray ionization from sea level to 1540 kilometers altitude

An air filled ionization chamber has been constructed with a volume of 552 liters and a wall consisting of 12.7 mg/cm² of plastic wrapped over a rigid, lightweight aluminum frame. A calibration in absolute units, independent of previous Caltech ion chamber calibrations, was applied to a sealed Neher electrometer for use in this chamber. The new chamber was flown along with an older, argon filled, balloon type chamber in a C-135 aircraft from 1,000 to 40,000 feet altitude, and other measurements of sea level cosmic ray ionization were made, resulting in the value of 2.60 ± .03 ion pairs/cm³ sec atm) at sea level. The calibrations of the two instruments were found to agree within 1 percent, and the airplane data were consistent with previous balloon measurements in the upper atmosphere. Ionization due to radon gas in the atmosphere was investigated. Absolute ionization data in the lower atmosphere have been compared with results of other observers, and discrepancies have been discussed.

Data from a polar orbiting ion chamber on the OGO-II, IV spacecraft have been analyzed. The problem of radioactivity produced on the spacecraft during passes through high fluxes of trapped protons has been investigated, and some corrections determined. Quiet time ionization averages over the polar regions have been plotted as function of altitude, and an analytical fit is made to the data that gives a value of 10.4 ± 2.3 percent for the fractional part of the ionization at the top of the atmosphere due to splash albedo particles, although this result is shown to depend on an assumed angular distribution for the albedo particles. Comparisons with other albedo measurements are made. The data are shown to be consistent with balloon and interplanetary ionization measurements. The position of the cosmic ray knee is found to exhibit an altitude dependence, a North-South effect, and a small local time variation.

The brine and gas content of sea ice with attention to low salinities and high temperatures

Based on the well known sea ice phase diagram, equations are derived for determining the brine and gas content of sea Ice for high temperatures (range 0 to -2 °C) and low salinities. The presently widely used equations of Cox and Weeks (1982) are valid only for temperatures below -2°C. Fresh-water ice is used as a boundary condition for the equations. The relative salt concentrations in brine are_assumed to be the same as in normal (or standard) seawater. Two sets of equations are presented: 1) accurate formulae based on UNESCO standard sea water equations, and 2) approximate formulae based on general properties of weak solutions. The approximate formulae are not essentially different from the classical system which basically assumes the freezing point to be a linear function of fractional salt content. The agreement between the two approaches is excellent and the approximate system is good enough for most applications.

A report on the population structure of adult salmon and sea trout in the River Lune using scale samples - with reference to historical data.

During 1993, a comprehensive data set of scale readings, length and weight measurements was established for migratory salmonids on the River Lune. This information was collected using three methods of fish capture: 1. The Lune estuary commercial nets. 2. River Lune Forge weir fish trap. 3. River Lune rod catch scale returns. Additional information was contributed by the Kent, Leven and Duddon rod and commercial fisheries. The data shows that the salmon stock in 1993 was dominated by two year old smolts. This varies from year to year. The sea trout population displays a normal population curve in terms of numbers of fish in each age and weight class. The growth rate of salmon and sea trout is very similar even though salmon have the benefit of high sea feeding.

River Wyre salmon and sea trout spawning habitat restoration / creation project. Final report April 1995

Adult salmon and sea trout rod catches in the River Wyre have been subject to considerable variability over the years. Annual rod catches ranging from 6 to 401 have been reported since records began in 1905. It has long been suspected that the physical nature of the catchment, combined with anthropogenic influences, has resulted in a deleterious effect on the Wyre fishery. Acidification problems in the head water streams (Marshaw Wyre and Tarnbrook Wyre) have been reported and are thought to threaten salmon juvenile survival. The construction of Abbeystead Reservoir and an increased tendency towards rapidly rising water levels during storms (flashiness) 1 are thought to have a significant impact on spawning gravel quality and quantity, both of which are thought to be deteriorating. As part of an overall desire to maintain and improve the migratory salmonid population in the River Wyre, this project has been commissioned to investigate remedial action which may improve and enhance spawning success, leading to an eventual improvement in the status of adult stocks. The primary objective is to establish whether the quantity and/or quality of available spawning gravels are limiting migratory salmonid productivity. The investigations undertaken confirm the general observation that useable spawning gravels appear to be in short supply in the River Wyre, and may be the limiting factor influencing returning adult stock.

Review of NRA tracking projects on salmon, Salmo salar L., and sea trout, Salmo trutta L

The report briefly outlines the programme of the National Rivers Authority (NRA), placing the Fisheries programme in the context of the work of the NRA as a whole, and viewing the tracking work against the broader requirements of the NRA Fisheries research programme. All regions of England and Wales are considered. Two techniques currently exist for studying the detailed timing and extent of movements of adult salmon: tracking of individually identifiable fish, and counting the numbers of fish moving past a fixed point in the river. The development of tracking techniques and the integrated use of tracking and fish counters is briefly reviewed in Section 3. Further details of these techniques are given in Appendices. Section 4 summarises and assesses completed and current NRA tracking studies. Section 5 discusses the scientific content of these studies in relation to similar work carried out elsewhere in the UK. The NRA programme of tracking studies is evaluated in Section 6. Section 7 discusses future fisheries projects and Section 8 details the future development of tracking techniques. Finally, recommendations arising out of this review are summarised in Section 9.

La gestión de la materia prima ósea en la fabricación de objetos durante la Prehistoria

Homenaje a Ignacio Barandiarán Maestu / coord. por Javier Fernández Eraso, Juan Santos Yanguas

A guide to the interpretation of sea trout scales

The overall purpose of this guide is to provide a manual for the collection and interpretation of sea trout scales. A brief introduction considers the advantages and disadvantages of using scales to determine age and growth. To ensure that scales are interpreted in a consistent manner, all major terms are defined and a standard system for age notation is proposed. The methodology for the collection, mounting and interpretation of scales is described in detail, and this is followed by a section on the back-calculation of lengths at different ages. Each topic is discussed critically. The final part of this guide is an atlas illustrating scales from a wide range of sea trout and including not only excellent "type-scales" but also difficult and impossible scales.

La industria ósea del Paleolítico superior de la cueva de Gatzarria (Zuberoa, País Vasco)

[ES] La cueva de Gatzarria ofrece un rico e interesante relleno del Paleolítico medio y superior. La industria ósea «elaborada» (puntas,puntas en extremo, alisadores y varillas) se halla documentada en los niveles Protoauriñacienses (cjn2 y cjnl), en el Auriñaciense antiguo de cbci-cbf y en el Autiñaciense evolucionado de cb. De todas las categorías atestiguadas que aquí tratamos, el grupo más numeroso e interesante es el de las puntas: junto a una serie de ejemplares sin denominación específica que se repiten en los mencionados niveles, existen algunos tipos ligados específicamente a algunos de ellos; es el caso de las puntas fusiformes en cjn2, de las puntas sublosángicas en cjnl y de las puntas de base hendida en cbci-cbf.

River Wyre salmon and sea trout spawning habitat restoration / creation project. Gravel enhancement schemes.

As part of an overall desire to maintain and improve the migratory salmonid population in the River Wyre, a project was commissioned to investigate remedial action which may improve and enhance spawning success and lead to an eventual improvement in the status of adult stocks. The report, titled "River Wyre Salmon and Sea Trout Spawning Habitat Restoration/Creation Project", concluded that usable spawning gravels appeared to be in short supply in the River Wyre catchment and may be the limiting factor influencing the returning adult stock, as juvenile nursery habitat was not found to be limiting. This report looks at gravel enhancement schemes to improve River Wyre salmon and sea trout spawning habitat restoration. It examines methods for gravel enhancement schemes: mechanical cleaning and construction of gravel addition structures. Includes sites figures of sites identified for enhancement.