Comparative genome maps of the pangolin, hedgehog, sloth, anteater and human revealed by cross-species chromosome painting: further insight into the ancestral karyotype and genome evolution of eutherian mammals

To better understand the evolution of genome organization of eutherian mammals, comparative maps based on chromosome painting have been constructed between human and representative species of three eutherian orders: Xenarthra, Pholidota, and Eulipotyphla,

Pipeline uplift response in thawed sandy backfills: Preliminary findings

Offshore and onshore buried pipelines under high operating temperature and pressures may lead to upheaval buckling (UHB) if sufficient soil cover is not present to prevent the upward movement of the pipeline. In regions where seasonal changes involve ground soil undergoing freezing-thawing cycles, the uplift resistance from soil cover may be minimum when the soil is undergoing thawing. This paper presents the results from 2 directly-comparable minidrum centrifuge tests conducted at the Schofield Centre, University of Cambridge, to investigate the difference in uplift resistance responses between fully-saturated and thawed sandy backfill conditions. Both tests were conducted drained at 30g using an 8.6 mm diameter aluminium model pipe, corresponding to a prototype pipe diameter of 258 mm. The soil cover/pipe diameter ratio, H/D, was kept at 1. Fraction E fine silica sand was used as the backfill. Preliminary experimental results indicated that the ultimate uplift resistance of a thawing sand backfill to be lower than that of a fully saturated sand backfill. This suggests that in regions where backfill soil undergoes freeze-thaw cycles, the thawing backfill may be more critical than fully saturated backfill for uplift resistance. The 2-dimensional displacement field during the experiment was accurately measured and analysed using the Particle Image Velocimetry technique. Copyright © 2011 by the International Society of Offshore and Polar Engineers (ISOPE).

Ecology and production in two sandy beaches of Goa. Part (A) Distribution of bacteria in Colva and Siridaon in relation to certain environmental parameters

Qualitative and quantitative studies on the bacterial flora of two beaches viz., Colva and Siridaon at high tide, mid-tide and low tide levels during the pre-monsoon period were made. Estimations of important nutrients, like inorganic phosphates and nitrates and organic carbon have been made and correlation with the bacterial counts is being attempted. Definite correlation between bacterial population and organic carbon is seen however, no correlation is observed between bacterial counts and other physicochemical parameters.

Phylogenetic relationships of five pika species (genus Ochotona) based on mitochondrial DNA restriction maps

Restriction site mapping of mitochondrial DNA (mtDNA) with 16 restriction endonucleases was used to examine the phylogenetic relationships of Ochotona cansus, O. huangensis, O. thibetana, O. curzoniae and O. erythrotis. A 1-kb length variation between O. erythrotis of subgenus Pika and other four species of subgenus Ochotona was observed, which may be a useful genetic marker for identifying the two subgenera. The phylogenetic tree constructed using PAUP based on 61 phylogenetically informative sites suggests that O. erythrotis diverged first, followed by O. cansus, while O. curzoniae and O. huangensis are sister taxa related to O. thibetana, The results indicate that both O. cansus and O. huangensis should be treated as independent species. If the base substitution rate of pikas mtDNA was 2% per million years, then the divergence time of the two subgenera, Pika and Ochotana, is about 8.8 Ma ago of late Miocence, middle Bao-dian of Chinese mammalian age, and the divergence of the four species in subgenus Ochotona would have occurred about 2.5 - 4.2 Ma ago, Yushean of Chinese mammalian age. This calculation appears to be substantiated by the fossil record.

Studies on Thiobacilli spp. isolated from sandy beaches of Kerala

Occurrence, isolation and oxidative activity of Thiobacilli spp. from some sandy beaches of Kerala are reported. These organisms were encountered in polluted beaches and were dominant during monsoon in all the beaches.

Phylogeny of Muntiacus (Cervidae) based on mitochondrial DNA restriction maps

Mitochondrial DNA restriction maps for 12 restriction enzymes of four species of muntjacs-Indian muntjac (M. muntjak), Gongshan muntjac (M. gongshanensis), black muntjac (M. crinifrons), and Chinese muntjac (M. reevesi)-were compared to estimate the phylogenetic relationships among them. Phylogenetic trees were constructed by both distance and parsimony methods. The two resulting trees share a similar topology, which indicates that the black muntjac and the Gongshan muntjac are closely related, followed by the Chinese muntjac; the Indian muntjac is the sister taxon to all the other muntjacs.