941 resultados para Popular mobilization


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UANL

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UANL

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UANL

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CD4+ T lymphocytes play an important role in CD8+ T cell-mediated responses against tumors. Considering that about 20% of melanomas express major histocompatibility complex (MHC) class II, it is plausible that concomitant antigenic presentation by MHC class I and class II complexes shapes positive (helper T cells) or negative (regulatory T cells) anti-tumor responses. Interestingly, gp100, a melanoma antigen, can be presented by both MHC class I and class II when expressed endogenously, suggesting that it can reach endosomal/MHC class II compartments (MIIC). Here, we demonstrated that the gp100 putative amino-terminal signal sequence and the last 70 residues in carboxy-terminus, are essential for MIIC localization and MHC class II presentation. Confocal microscopy analyses confirmed that gp100 was localized in LAMP-1+ endosomal/MIIC. Gp100-targeting sequences were characterized by deleting different sections in the carboxy-terminus (residues 590 to 661). Transfection in 293T cells, expressing MHC class I and class II molecules, revealed that specific deletions in carboxy-terminus resulted in decreased MHC class II presentation, without effects on MHC class I presentation, suggesting a role in MIIC trafficking for these deleted sections. Then, we used these gp100-targeting sequences to mobilize the green fluorescent protein (GFP) to endosomal compartments, and to allow MHC class II and class I presentation of minimal endogenous epitopes. Thus, we concluded that these specific sequences are MIIC targeting motifs. Consequently, these sequences could be included in expression cassettes for endogenously expressed tumor or viral antigens to promote MHC class II and class I presentation and optimize in vivo T cell responses, or as an in vitro tool for characterization of new MHC class II epitopes.

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Tesis (Doctorado en Ciencias con Acentuación en Alimentos) UANL, 2013.

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Why did a majority of French voters reject the Treaty Establishing a Constitution for Europe in the 2005 referendum? We argue here that the collective mobilization of the left-wing ‘No’ camp made the decisive difference through its formation of ‘Collectifs pour le non’, a coalition which facilitated the public expression of an anti-liberal and pro-European position capable of bringing together the Green and Socialist electorates, along with other parts of the left. Using a comprehensive analysis of the multi-organizational field of protest constituted by the mobilization of the left-wing ‘No’ camp, we show first that the mobilization was a ‘European affair’, in the sense that it developed a pro-European position in the context of struggles against liberal forces. Second, we show that the mobilization was also a ‘French’ affair because it relied on the high valuation of the ‘national’ mode of belonging, through the defence of the French state model.

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Se presenta experiencia educativa que propone hacer un recorrido por la historia y los cantes flamencos para fomentar la cultura popular andaluza entre el alumnado. Se realiza en el IES Fray Luis de Granada en Granada. Los objetivos son: tener una noción de la historia del flamenco, lo que en gran medida equivale a tener una noción sobre la historia de Andalucía; situar al flamenco en su contexto artístico, conocer la relación que han tenido con el flamenco pintores como Romero de Torres, músicos como Manuel de Falla, o escritores como los Machado, Lorca, los románticos, Salvador Rueda, Alberti, Caballero Bonald, Félix Grande, etc; tener alguna noción de algunos cantes en los que se divide el flamenco. Su ritmo, métrica, temática, y otras características esenciales; apreciar el cante en directo, sirviéndonos de él para ilustrar las explicaciones teóricas.