Genomic Signatures Predict Poor Outcome in Undifferentiated Pleomorphic Sarcomas and Leiomyosarcomas

Undifferentiated high-grade pleomorphic sarcomas (UPSs) display aggressive clinical behavior and frequently develop local recurrence and distant metastasis. Because these sarcomas often share similar morphological patterns with other tumors, particularly leiomyosarcomas (LMSs), classification by exclusion is frequently used. In this study, array-based comparative genomic hybridization (array CGH) was used to analyze 20 UPS and 17 LMS samples from untreated patients. The LMS samples presented a lower frequency of genomic alterations compared with the UPS samples. The most frequently altered UPS regions involved gains at 20q13.33 and 7q22.1 and losses at 3p26.3. Gains at 8q24.3 and 19q13.12 and losses at 9p21.3 were frequently detected in the LMS samples. Of these regions, gains at 1q21.3, 11q12.2-q12.3, 16p11.2, and 19q13.12 were significantly associated with reduced overall survival times in LMS patients. A multivariate analysis revealed that gains at 1q21.3 were an independent prognostic marker of shorter survival times in LMS patients (HR = 13.76; P = 0.019). Although the copy number profiles of the UPS and LMS samples could not be distinguished using unsupervised hierarchical clustering analysis, one of the three clusters presented cases associated with poor prognostic outcome (P = 0.022). A relative copy number analysis for the ARNT, SLC27A3, and PBXIP1 genes was performed using quantitative real-time PCR in 11 LMS and 16 UPS samples. Gains at 1q21-q22 were observed in both tumor types, particularly in the UPS samples. These findings provide strong evidence for the existence of a genomic signature to predict poor outcome in a subset of UPS and LMS patients. © 2013 Silveira et al.

Ki-67 and CD100 immunohistochemical expression is associated with local recurrence and poor prognosis in soft tissue sarcomas, respectively

Soft tissue sarcomas (STSs) are a heterogeneous group of mesenchymal tumors of >50 subtypes. However, STSs represent <1% of types of cancer. Despite this low frequency, the disease is aggressive and treatment, when possible, is based on traditional chemotherapies. A number of cases of resistance to adjuvant therapies have been reported. Metastases are commonly identified in STS patients during diagnosis and the development of effective clinical parameters is crucial for correct management of the disease. The use of biological markers in cancer is a useful tool to determine patient prognosis. Ki--67 is a protein marker for proliferation of somatic cells and is widely used in prognostic studies of various types of tumor, including STSs. Cluster of differentiation 100 (CD100) is a member of the semaphorin family. The family was initially described as axon guidance molecules important for angiogenesis, organogenesis, apoptosis and neoplasia. CD100 was previously utilized as a prognostic factor in tumors and also in STSs. In the present study, protein expression of Ki--67 and CD100 was analyzed by immunohistochemistry in samples of STS patients of the Barretos Cancer Hospital (Barretos, Brazil) to establish prognostic criteria of the disease. Results demonstrate a correlation between CD100 expression and poor prognosis, consistent with a previous study. Moreover, the expression of Ki-67 was identified to correlate with presence of local or locoregional recurrence. To the best of our knowledge, no large casuistic study has revealed this correlation between Ki--67 and local recurrence in STSs. The use of Ki--67 and CD100 as markers in clinical pathological analysis may be suitable as a prognostic criterion in disease progression.

Water supply and sanitation for the poor: the achievements of the International Drinking Water Supply and Sanitation Decade in Latin America and the Caribbean

Includes bibliography

Apoyo a la productividad de los pobres rurales: nuevas experiencias en América Latina y el Caribe = Support for the productivity of the rural poor: new experiences in Latin America and the Caribbean

Incluye Bibliografía

Pobres, urbanos y productividad: claves de intervención = Urban poor and productivity: keys to action

Incluye Bibliografía

Urban poor and productivity: keys to action. Public programmes aimed at raising productivity of poor sectors in urban areas of Jamaica

Includes bibliography

Apoyo a la productividad de los pobres rurales: nuevas experiencias en América Latina y el Caribe: current experience in increasing the productivity level among the poor sector in rural areas of Dominica: the case of Petite Savanne

Includes bibliography

Water supply and sanitation for the poor: the achievements of the International Drinking Water Supply and Sanitation Decade in Latin America and the Caribbean = Abastecimiento de agua y saneamiento ambiental para los pobres: los logros del Decenio Internacional del Agua Potable y del Saneamiento Ambiental en América Latina y el Caribe

Incluye Bibliografía

The transport systems of Latin American cities: how they might better serve the needs of the poor

Includes bibliography

The urban poor in Brazil in 1996: a new poverty profile using PPV, PNAD and census data

Includes bibliography

Transport, the poor and moving towards low-carbon societies

This FAL Bulletin discusses the challenges involved in creating equality in urban mobility and the need to reach towards a social sustainability framework, as existing policies often accentuate current inequities and inequalities in accessibility. Lack of access to mobility has wide-ranging effects across social groups, gender and particularly the urban poor.