Adenovirus-mediated siRNA targeting TNF-α and overexpression of bone morphogenetic protein-2 promotes early osteoblast differentiation on a cell model of Ti particle-induced inflammatory response in vitro

Wear particles are phagocytosed by macrophages and other inflammatory cells, resulting in cellular activation and release of proinflammatory factors, which cause periprosthetic osteolysis and subsequent aseptic loosening, the most common causes of total joint arthroplasty failure. During this pathological process, tumor necrosis factor-alpha (TNF-α) plays an important role in wear-particle-induced osteolysis. In this study, recombination adenovirus (Ad) vectors carrying both target genes [TNF-α small interfering RNA (TNF-α-siRNA) and bone morphogenetic protein 2 (BMP-2)] were synthesized and transfected into RAW264.7 macrophages and pro-osteoblastic MC3T3-E1 cells, respectively. The target gene BMP-2, expressed on pro-osteoblastic MC3T3-E1 cells and silenced by the TNF-α gene on cells, was treated with titanium (Ti) particles that were assessed by real-time PCR and Western blot. We showed that recombinant adenovirus (Ad-siTNFα-BMP-2) can induce osteoblast differentiation when treated with conditioned medium (CM) containing RAW264.7 macrophages challenged with a combination of Ti particles and Ad-siTNFα-BMP-2 (Ti-ad CM) assessed by alkaline phosphatase activity. The receptor activator of nuclear factor-κB ligand was downregulated in pro-osteoblastic MC3T3-E1 cells treated with Ti-ad CM in comparison with conditioned medium of RAW264.7 macrophages challenged with Ti particles (Ti CM). We suggest that Ad-siTNFα-BMP-2 induced osteoblast differentiation and inhibited osteoclastogenesis on a cell model of a Ti particle-induced inflammatory response, which may provide a novel approach for the treatment of periprosthetic osteolysis.

天父上帝言題皇詔Tian fu shang di yan ti huang zhao.Décrets et pièces diverses publiés par les Tai ping.

Recueil de 13 pièces officielles de la rébellion, datées de 1851, 1852, 1853, avec une table.

乾坤體義Qian kun ti yi.Principes du système du monde.

Cat. imp., liv. 106, f. 16 (en 2 livres).Par le P. Ricci (1552-1610). Texte relatif à la cosmographie et à la géographie, avec figures.

天主一體三位論Tian zhu yi ti san wei lun.

Traité de la Sainte Trinité.Par le P. Monteiro.9 feuillets.

三位一體說San wei yi ti shuo ; alias : 三一聖學San yi sheng xue.

Traité de la Sainte Trinité.Attribué au P. Buglio ; préface sans signature ni date.47 sections.

提正編Ti zheng bian.

Considérations sur les mystères de la foi.Par le P. Jérôme de Gravina, Jésuite (1603-1662 ; noms Chinois Jia Yi mu Jiu zhang). Gravé à l'église de Hai yu avec l'autorisation du P. Simon da Cunha, Jésuite (1587-1660 ; noms Chinois Qu Xi man Fu yi). Préface (1659) par Tong Guo qi, de Dai yuan.6 livres.

聖體要理Sheng ti yao li.

Même ouvrage avec l'autorisation du P. Furtado ; le titre indique la date de 1644 et l'église Ji shan. Vraisemblablement reproduction moderne. La dernière section du livre 2 est supprimée.

聖體荅疑Sheng ti da yi.

Réponse aux doutes sur l'Eucharistie.Par le P. Verbiest. Table et texte.11 feuillets.

聖體仁愛經規條Sheng ti ren ai jing gui tiao.

Exercices et règlement pour la confrérie du Saint Sacrement.Par le P. de Mailla ; préface (1719) et postface de l'auteur. Table. Édition de l'église Shou shan, à Pékin.52 feuillets.

聖體會規Sheng ti hui gui.

Règlement de la confrérie du Saint Sacrement.13 feuillets.

聖體問荅Sheng ti wen da.

Instruction dialoguée sur l'eucharistie.6 feuillets.

The diffusion of Co⠶溩 Zrâ â Tiâ â alloy /|nS. Kumar. -- 260 St Catharines [Ont.] : Dept. of Physics, Brock University,

The diffusion of Co60 in the body centered cubic beta phase of a ZrSOTi SO alloy has been studied at 900°, 1200°, and 1440°C. The results confirm earlier unpublished data obtained by Kidson17 • The temperature dependence of the diffusion coefficient is unusual and suggests that at least two and possibly three mechanisms may be operative Annealing of the specimen in the high B.C.C. region prior to the deposition of the tracer results in a large reduction in the diffusion coefficient. The possible significance of this effect is discussed in terms of rapid transport along dislocation network.

Estudio in vivo de oseointegración en implantes dentales de Ti-6AI-4V con superficies modificadas.

Tesis (Maestro en Ciencias de la Ingeniería Mecánica con Especialidad en Materiales) UANL, 2013.