Display of a Maize cDNA library on baculovirus infected insect cells

Background: Maize is a good model system for cereal crop genetics and development because of its rich genetic heritage and well-characterized morphology. The sequencing of its genome is well advanced, and new technologies for efficient proteomic analysis are needed. Baculovirus expression systems have been used for the last twenty years to express in insect cells a wide variety of eukaryotic proteins that require complex folding or extensive posttranslational modification. More recently, baculovirus display technologies based on the expression of foreign sequences on the surface of Autographa californica ( AcMNPV) have been developed. We investigated the potential of a display methodology for a cDNA library of maize young seedlings. Results: We constructed a full-length cDNA library of young maize etiolated seedlings in the transfer vector pAcTMVSVG. The library contained a total of 2.5 x 10(5) independent clones. Expression of two known maize proteins, calreticulin and auxin binding protein (ABPI), was shown by western blot analysis of protein extracts from insect cells infected with the cDNA library. Display of the two proteins in infected insect cells was shown by selective biopanning using magnetic cell sorting and demonstrated proof of concept that the baculovirus maize cDNA display library could be used to identify and isolate proteins. Conclusion: The maize cDNA library constructed in this study relies on the novel technology of baculovirus display and is unique in currently published cDNA libraries. Produced to demonstrate proof of principle, it opens the way for the development of a eukaryotic in vivo display tool which would be ideally suited for rapid screening of the maize proteome for binding partners, such as proteins involved in hormone regulation or defence.

Dear living person

‘Dear Living Person,’ Chapter included in edited collection incl. texts by Claire Fontaine, Gareth James, Paul Chan and Sam Lewitt. The chapter is written from the perspective of a recently deceased corpse. It examines the claims art makes to moving beyond boundaries and testing limits (of medium, discipline, and category) as a cliché of transcendence. This is described in relation to the operation of the false ‘master’ limit between the living and the dead, with reference to Reza Negarestani’s vivid reenactment of Etruscan torture, and contemporary performances of institutional critique: Fraser, Krebber and Carpenter.

The world of emotions is not two-dimensional

For more than half a century, emotion researchers have attempted to establish the dimensional space that most economically accounts for similarities and differences in emotional experience. Today, many researchers focus exclusively on two-dimensional models involving valence and arousal. Adopting a theoretically based approach, we show for three languages that four dimensions are needed to satisfactorily represent similarities and differences in the meaning of emotion words. In order of importance, these dimensions are evaluation-pleasantness, potency-control, activation-arousal, and unpredictability. They were identified on the basis of the applicability of 144 features representing the six components of emotions: (a) appraisals of events, (b) psychophysiological changes, (c) motor expressions, (d) action tendencies, (e) subjective experiences, and (f) emotion regulation.

Framtagning av lämplig teknologi för konvertering av VBA-applikation till Internet Explorer

Företaget GUTAB använder idag en frågeanalysmodul som är utvecklat i Visual Basic for Applications i Microsoft Excel. GUTAB fann det intressant att veta om modulen kunde konverteras till webbläsaren Internet Explorer samt vilken teknologi som var lämplig för detta. GUTAB var även intresserade av att veta vilken teknologi som kunde användas för att skapa grafiska manipulerbara objekt.En skrivbordsundersökning genomfördes som omfattade teknologierna ASP, ASP.NET, JSP, PHP, ActiveX samt Java applets. Skrivbordsundersökningen utgjordes av två delar där den första delen redogjorde för de två klientbaserade teknologierna ActiveX och Java applets med vilka grafiska manipulerbara objekt kunde realiseras. GUTAB ansåg att ActiveX program var mest lämpliga för detta ändamål.Baserat på skrivbordsundersökningens andra del bestämde GUTAB att ASP och ASP.NET skulle användas för att testa teknologiernas kapacitet att konvertera programlogik samt grafiska objekt från VBA-applikationen i en webbapplikation då dessa två teknologier ansågs som mest lämpliga för en konvertering.Testet som genomfördes visade att det var fullt möjligt att konvertera utvald funktionalitet med båda teknologierna. Dock kunde inte grafiska objekt skapas i asp varför befintliga objekt användes för det ändamålet. Många fördelar identifierades med ASP.NET varför GUTAB beslutade att ASP.net var den mest lämpliga teknologin att använda vid en eventuell konvertering.

Schemaläggningsalgoritm för arbetstidsschema

Programmet schemaläggaren har funnits sedan 1994, och används på arbetsplatser för att på ett strukturerat sätt lägga scheman för de anställda. Programmet har sedan starten varit sådant att man själv ska skapa alla scheman och även koppla dessa till de anställda, men det finns en önskan att programmet ska vara mer användarvänligt och lättanvänt. Ett steg i utvecklingen är att skapa en automatisk schemaläggningsguide som gör det mesta av jobbet åt användaren.Etex AB, som detta examensarbete är gjort åt, avser att med detta examensarbete skapa en prototyp på en sådan schemaläggningsguide som ska implementeras i det befintliga programmet. Denna guide ska alltså skapa scheman utifrån given data och sedan koppla dessa scheman till de anställda som valts. Allt detta ska ske automatiskt. Efter detta har man sedan möjlighet att antingen spara de scheman och kopplingar som gjorts, eller avvisa dem.Grundläggande tanken med denna modul är att den ska passa in i det befintliga programmet utan att vara bundet till det, så man ska kunna överföra den till en annan applikation om nödvändigt. Trots vissa komplikationer efter vägen har arbetet ändå slutförts och med fortsatt arbete kan modulen bli en bra del av programmet Schemaläggaren.

Utveckling av en ny modul i WM-datas arbetsorderhanteringssystem för registrering av snöröjning respektive sandning

WM-data tillhandahåller en lösning för mobil arbetsorderhantering. Lösningen innehåller planeringsverktyg med ärenden, personal och karta och möjlighet att leverera arbetsorder till fältpersonal. Den innehåller dessutom handhållna mobilenheter, vilka fältpersonalen använder för att ta emot aktuella arbetsorder.Vissa kunder önskar ytterligare en modul i denna mobila lösning. Den nya modulen skall användas för att kontinuerligt rapportera in genomförda åtgärder i snöröjningssammanhang.Detta examensarbete går ut på att utveckla den här modulen för att registrera snöröjning respektive sandning. Applikationen exekveras på en PDA, en så kallad Pocket PC med Windows Mobile 5.0. Anledningen till att just Windows Mobile 5.0 skall användas är att detta operativsystem har inbyggt stöd för GPS-positionering. Ett önskemål av WM-data var att detta inbyggda stöd skulle undersökas, rapporteras och användas i applikationen.Resultatet blev en applikation där man via en Pocket PC kontinuerligt sparar undan fordons GPS-position, arbetsåtgärd samt tidpunkterna när GPS-positionerna togs. Denna information är sedan anpassad för att kunna skickas vidare via GPRS till en befintlig databasserver under vissa tidsintervaller.Applikationen utvecklades med hjälp av programmeringsspråket C Sharp (C#) i Visual Studio 2005.

Achados da fundoscopia e alterações do pé diabético em pacientes do Hospital Universitário Onofre Lopes/UFRN

Trabalho com o objetivo de identificar as alterações do pé diabético causadas pelas lesões microangiopáticas e das lesões do fundo de olho secundárias aretinopatia diabética. Métodos:76 pacientes com Diabetes Melito tipos 1 e 2atendidos no ambulatório de Oftalmologia e Cirurgia Vascular do HUOL/UFRN, Natal, RN, no período de novembro de 2004 a janeiro de 2005, com queixas relativas a alterações da retinopatia diabéticae/oudo pé diabético. Em todos os pacientes foi realizado exame clínico geral, vascular e oftalmológico. Na avaliação específicado pé diabético deu-se ênfase paraa investigação do status vascular pela Classificação de Fontaine para Doença Arterial Obstrutiva Periférica, biomecânica,e teste do monofilamento de Semmes-Weinstein. O exame oftalmológico constou de refração e fundoscopiaatravés da qual identificou-se as formas clínicas da retinopatia diabética. Os dados foram submetidos à análise estatística das variáveis primárias que consistiu em caracterizar o grupo quanto a idade, tempo de doença, nível de glicose A segunda estratégia da análise dos dados constituiu na realização de testes de associação entrealgumas variáveis secundárias selecionadas. O software utilizado para os testes estatísticos foi o Statistica Versão 5, 1997.Resultado: Dos 76 pacientes diabéticos 97% tinham idade superior a 40 anos. O tempo de doença65% tinham mais de 10 anos. Com relação à glicose 72,72% apresentaram níveis de glicose em jejum acima de 100mg/dl. 55,26% apresentavam algum grau de retinopatia diabética contra 44,74% que não apresentavamesses sinais. Com as alterações do pé diabético, identificou-se 59,93% com lesões com área de predominância isquêmica, enquanto 41,07% tinham ausência de sinais. 58,82% apresentaram área de predominância neuropática, e 41,18% sem sinais de neuropatia. Dos com retinopatia diabética 78,57% tinham comprometimento isquêmico no pé e 47,62% tinham algum grau de neuropatia diabética. Observou-se que a retinopatia diabética não proliferativa, nos seus diversos graus de comprometimento apresentou-se com percentuais em torno de 80% junto às lesões do pé diabético, seja isquêmico ou neuropático. Dos pacientes que tinham retinopatia 60,46% tinham alterações biomecânicas dos pés. Conclusão: Concluiu-se que a RDNP leve foi mais freqüente nas lesões do pé diabético isquêmico, enquanto a RDNP severa mostrou-se mais presente no pé diabético neuropático

CMS physics technical design report, volume II: Physics performance

CMS is a general purpose experiment, designed to study the physics of pp collisions at 14 TeV at the Large Hadron Collider ( LHC). It currently involves more than 2000 physicists from more than 150 institutes and 37 countries. The LHC will provide extraordinary opportunities for particle physics based on its unprecedented collision energy and luminosity when it begins operation in 2007. The principal aim of this report is to present the strategy of CMS to explore the rich physics programme offered by the LHC. This volume demonstrates the physics capability of the CMS experiment. The prime goals of CMS are to explore physics at the TeV scale and to study the mechanism of electroweak symmetry breaking - through the discovery of the Higgs particle or otherwise. To carry out this task, CMS must be prepared to search for new particles, such as the Higgs boson or supersymmetric partners of the Standard Model particles, from the start- up of the LHC since new physics at the TeV scale may manifest itself with modest data samples of the order of a few fb(-1) or less. The analysis tools that have been developed are applied to study in great detail and with all the methodology of performing an analysis on CMS data specific benchmark processes upon which to gauge the performance of CMS. These processes cover several Higgs boson decay channels, the production and decay of new particles such as Z' and supersymmetric particles, B-s production and processes in heavy ion collisions. The simulation of these benchmark processes includes subtle effects such as possible detector miscalibration and misalignment. Besides these benchmark processes, the physics reach of CMS is studied for a large number of signatures arising in the Standard Model and also in theories beyond the Standard Model for integrated luminosities ranging from 1 fb(-1) to 30 fb(-1). The Standard Model processes include QCD, B-physics, diffraction, detailed studies of the top quark properties, and electroweak physics topics such as the W and Z(0) boson properties. The production and decay of the Higgs particle is studied for many observable decays, and the precision with which the Higgs boson properties can be derived is determined. About ten different supersymmetry benchmark points are analysed using full simulation. The CMS discovery reach is evaluated in the SUSY parameter space covering a large variety of decay signatures. Furthermore, the discovery reach for a plethora of alternative models for new physics is explored, notably extra dimensions, new vector boson high mass states, little Higgs models, technicolour and others. Methods to discriminate between models have been investigated. This report is organized as follows. Chapter 1, the Introduction, describes the context of this document. Chapters 2-6 describe examples of full analyses, with photons, electrons, muons, jets, missing E-T, B-mesons and tau's, and for quarkonia in heavy ion collisions. Chapters 7-15 describe the physics reach for Standard Model processes, Higgs discovery and searches for new physics beyond the Standard Model.

Search for narrow resonances in dilepton mass spectra in pp collisions at root s=7 TeV CMS Collaboration

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Search for the standard model Higgs boson decaying to bottom quarks in pp collisions at root s=7 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

First measurement of hadronic event shapes in pp collisions at root s=7 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Search for charge-asymmetric production of W ' bosons in t(t)over-bar + jet events from pp collisions at root s=7 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Search for physics beyond the standard model using multilepton signatures in pp collisions at root s=7 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)