Dense Map Inference with User-Defined Priors: From Priorlets to Scan Eigenvariations

When mapping is formulated in a Bayesian framework, the need of specifying a prior for the environment arises naturally. However, so far, the use of a particular structure prior has been coupled to working with a particular representation. We describe a system that supports inference with multiple priors while keeping the same dense representation. The priors are rigorously described by the user in a domain-specific language. Even though we work very close to the measurement space, we are able to represent structure constraints with the same expressivity as methods based on geometric primitives. This approach allows the intrinsic degrees of freedom of the environment’s shape to be recovered. Experiments with simulated and real data sets will be presented

Identification of logically related heap regions

This paper introduces a novel technique for identifying logically related sections of the heap such as recursive data structures, objects that are part of the same multi-component structure, and related groups of objects stored in the same collection/array. When combined withthe lifetime properties of these structures, this information can be used to drive a range of program optimizations including pool allocation, object co-location, static deallocation, and region-based garbage collection. The technique outlined in this paper also improves the efficiency of the static analysis by providing a normal form for the abstract models (speeding the convergence of the static analysis). We focus on two techniques for grouping parts of the heap. The first is a technique for precisely identifying recursive data structures in object-oriented programs based on the types declared in the program. The second technique is a novel method for grouping objects that make up the same composite structure and that allows us to partition the objects stored in a collection/array into groups based on a similarity relation. We provide a parametric component in the similarity relation in order to support specific analysis applications (such as a numeric analysis which would need to partition the objects based on numeric properties of the fields). Using the Barnes-Hut benchmark from the JOlden suite we show how these grouping methods can be used to identify various types of logical structures allowing the application of many region-based program optimizations.

A core ontological model for semantic sensor web infrastructures

Semantic Sensor Web infrastructures use ontology-based models to represent the data that they manage; however, up to now, these ontological models do not allow representing all the characteristics of distributed, heterogeneous, and web-accessible sensor data. This paper describes a core ontological model for Semantic Sensor Web infrastructures that covers these characteristics and that has been built with a focus on reusability. This ontological model is composed of different modules that deal, on the one hand, with infrastructure data and, on the other hand, with data from a specific domain, that is, the coastal flood emergency planning domain. The paper also presents a set of guidelines, followed during the ontological model development, to satisfy a common set of requirements related to modelling domain-specific features of interest and properties. In addition, the paper includes the results obtained after an exhaustive evaluation of the developed ontologies along different aspects (i.e., vocabulary, syntax, structure, semantics, representation, and context).

Virtual Excursions: a new way to explore science in class

The educational platform Virtual Science Hub (ViSH) has been developed as part of the GLOBAL excursion European project. ViSH (http://vishub.org/) is a portal where teachers and scientist interact to create virtual excursions to science infrastructures. The main motivation behind the project was to connect teachers - and in consequence their students - to scientific institutions and their wide amount of infrastructures and resources they are working with. Thus the idea of a hub was born that would allow the two worlds of scientists and teachers to connect and to innovate science teaching. The core of the ViSH?s concept design is based on virtual excursions, which allow for a number of pedagogical models to be applied. According to our internal definition a virtual excursion is a tour through some digital context by teachers and pupils on a given topic that is attractive and has an educational purpose. Inquiry-based learning, project-based and problem-based learning are the most prominent approaches that a virtual excursion may serve. The domain specific resources and scientific infrastructures currently available on the ViSH are focusing on life sciences, nano-technology, biotechnology, grid and volunteer computing. The virtual excursion approach allows an easy combination of these resources into interdisciplinary teaching scenarios. In addition, social networking features support the users in collaborating and communicating in relation to these excursions and thus create a community of interest for innovative science teaching. The design and development phases were performed following a participatory design approach. An important aspect in this process was to create design partnerships amongst all actors involved, researchers, developers, infrastructure providers, teachers, social scientists, and pedagogical experts early in the project. A joint sense of ownership was created and important changes during the conceptual phase were implemented in the ViSH due to early user feedback. Technology-wise the ViSH is based on the latest web technologies in order to make it cross-platform compatible so that it works on several operative systems such as Windows, Mac or Linux and multi-device accessible, such as desktop, tablet and mobile devices. The platform has been developed in HTML5, the latest standard for web development, assuring that it can run on any modern browser. In addition to social networking features a core element on the ViSH is the virtual excursions editor. It is a web tool that allows teachers and scientists to create rich mash-ups of learning resources provided by the e-Infrastructures (i.e. remote laboratories and live webcams). These rich mash-ups can be presented in either slides or flashcards format. Taking advantage of the web architecture supported, additional powerful components have been integrated like a recommendation engine to provide personalized suggestions about educational content or interesting users and a videoconference tool to enhance real-time collaboration like MashMeTV (http://www.mashme.tv/).

The Threads of Biosystems Engineering

The core concepts, or threads, of Biosystems Engineering (BSEN) are variously understood by those within the discipline, but have never been unequivocally defined due to its early stage of development. This makes communication and teaching difficult compared to other well established engineering subjects. Biosystems Engineering is a field of Engineering which int egrates engineering science and design with applied biological, environmental and agricultural sciences. It represents an evolution of the Agricultural Engineering discipline applied to all living organisms not including biomedical applications. The basic key element for the emerging EU Biosystems Engineering program of studies is to ensure that it offers essential minimum fundamental engine ering knowledge and competences . A core curriculum developed by Erasmus Thematic Networks is used as benchmark for Agr icultural and Biosystems Engineering studies in Europe. The common basis of the core curriculum for the discipline across the Atlantic , including a minimum of competences comprising the Biosystems Engineering core competencies, has been defined by an Atlan tis project , but this needs to be taken further by defining the threads linking courses together. This paper presents a structured approach to define the Threads of BSEN . The definition of the mid-level competences and the associated learning outcomes has been one of the objectives of the Atlantis programme TABE.NET. The mid-level competences and learning outcomes for each of six specializations of BSEN are defined while the domain-specific knowledge to be acquired for each outcome is proposed. Once the proposed definitions are adopted, these threads will be available for global development of the BSEN.

Vigilancia por análisis de ruido de los sensores de temperatura

En este proyecto se van a aplicar las técnicas de análisis de ruido para caracterizar la respuesta dinámica de varios sensores de temperatura, tanto termorresistencias de platino como de termopares. Estos sensores son imprescindibles para él correcto funcionamiento de las centrales nucleares y requieren vigilancia para garantizar la exactitud de las medidas. Las técnicas de análisis de ruido son técnicas pasivas, es decir, no afectan a la operación de la planta y permiten realizar una vigilancia in situ de los sensores. Para el caso de los sensores de temperatura, dado que se pueden asimilar a sistemas de primer orden, el parámetro fundamental a vigilar es el tiempo de respuesta. Éste puede obtenerse para cada una de las sondas por medio de técnicas en el dominio de la frecuencia (análisis espectral) o por medio de técnicas en el dominio del tiempo (modelos autorregresivos). Además de la estimación del tiempo de respuesta, se realizará una caracterización estadística de las sondas. El objetivo es conocer el comportamiento de los sensores y vigilarlos de manera que se puedan diagnosticar las averías aunque éstas estén en una etapa incipiente. ABSTRACT In this project we use noise analysis technique to study the dynamic response of RTDs (Resistant temperature detectors) and thermocouples. These sensors are essential for the proper functioning of nuclear power plants and therefore need to be monitored to guarantee accurate measurements. The noise analysis techniques do not affect plant operation and allow in situ monitoring of the sensors. Temperature sensors are equivalent to first order systems. In these systems the main parameter to monitor is the response time which can be obtained by means of techniques in the frequency domain (spectral analysis) as well as time domain (autoregressive models). Besides response time estimation the project will also include a statistical study of the probes. The goal is to understand the behavior of the sensors and monitor them in order to detect any anomalies or malfunctions even if they occur in an early stage.

Desarrollo de un compilador para el lenguaje de especificación de eventos en series temporales TESL

Este Trabajo de Fin de Grado recoge el diseño e implementación de un compilador y una librería de entorno de ejecución para el lenguaje específico del dominio TESL, un lenguaje de alto nivel para el análisis de series temporales diseñado por un grupo de investigación de la Universidad Politécnica de Madrid. Este compilador es el primer compilador completo disponible para TESL y sirve como base para la continuación del desarrollo del lenguaje, estando ideado para permitir su adaptación a cambios en el mismo. El compilador ha sido implementado en Java siguiendo la arquitectura clásica para este tipo de aplicaciones, incluyendo un Analizador Léxico, Sintáctico y Semántico, así como un Generador de Código. Se ha documentado su arquitectura y las decisiones de diseño que han conducido a la misma. Además, se ha demostrado su funcionamiento con un caso práctico de análisis de eventos en métricas de servidores. Por último, se ha documentado el lenguaje TESL, en cuyo desarrollo se ha colaborado. ---ABSTRACT---This Bachelor’s Thesis describes the design and implementation of a compiler and a runtime library for the domain-specific language TESL, a high-level language for analyzing time series events developed by a research group from the Technical University of Madrid. This is the first fully implemented TESL compiler, and serves as basis for the continuation of the development of the language. The compiler has been implemented in Java following the classical architecture for this kind of systems, having a four phase compilation with a Lexer, a Parser, a Semantic Analyzer and a Code Generator. Its architecture and the design decisions that lead to it have been documented. Its use has been demonstrated in an use-case in the domain of server metrics. Finally, the TESL language itself has been extended and documented.

Embedding logical frameworks in scala

El Framework Lógico de Edimburgo ha demostrado ser una poderosa herramienta en el estudio formal de sistemas deductivos, como por ejemplo lenguajes de programación. Sin embargo su principal implementación, el sistema Twelf, carece de expresividad, obligando al programador a escribir código repetitivo. Este proyecto presenta una manera alternativa de utilizar Twelf: a través de un EDSL (Lenguaje Embebido de Dominio Específico) en Scala que permite representar firmas del Framework Lógico, y apoyándonos en Twelf como backend para la verificación, abrimos la puerta a diversas posibilidades en términos de metaprogramación. El código fuente, así como instrucciones para instalar y configurar, está accesible en https://github.com/akathorn/elfcala. ---ABSTRACT---The Edinburgh Logical Framework has proven to be to be a powerful tool in the formal study of deductive systems, such as programming languages. However, its main implementation, the Twelf system, lacks expressiveness, requiring the programmer to write repetitive code. This project presents an alternative way of using Twelf: by providing a Scala EDSL (Embedded Domain Specific Language) that can encode Logical Framework signatures and relying on Twelf as a backend for the verification, we open the door to different possibilities in terms of metaprogramming. The source code, along with instructions to install and configure, is accessible at https://github.com/akathorn/elfcala

FATS: a framework for annotation of travel blogs based on subjectivity

This paper describes a framework for annotation on travel blogs based on subjectivity (FATS). The framework has the capability to auto-annotate -sentence by sentence- sections from blogs (posts) about travelling in the Spanish language. FATS is used in this experiment to annotate com- ponents from travel blogs in order to create a corpus of 300 annotated posts. Each subjective element in a sentence is annotated as positive or negative as appropriate. Currently correct annotations add up to about 95 per cent in our subset of the travel domain. By means of an iterative process of annotation we can create a subjectively annotated domain specific corpus.

Herramienta Web para el modelado de consultas CEP (Complex Event Processing)

RESUMEN En los últimos años, debido al incremento en la demanda por parte de las empresas de tecnologías que posibiliten la monitorización y el análisis de un gran volumen de datos en tiempo real, la tecnología CEP (Complex Event Processing) ha surgido como una potencia en alza y su uso se ha incrementado notablemente en ciertos sectores como, por ejemplo, la gestión y automatización de procesos de negocios, finanzas, monitorización de redes y aplicaciones, así como redes de sensores inteligentes como el caso de estudio en el que nos centraremos. CEP se basa en un lenguaje de procesamiento de eventos (Event Processing Language,EPL) cuya utilización puede resultar bastante compleja para usuarios inexpertos. Esta complejidad supone un hándicap y, por lo tanto, un problema a la hora de que su uso se extienda. Este Proyecto Fin de Grado (PFG) pretende dar una solución a este problema, acercando al usuario la tecnología CEP mediante técnicas de abstracción y modelado. Para ello, este PFG ha definido un lenguaje de modelado específico dominio, sencillo e intuitivo para el usuario inexperto, al que se ha dado soporte mediante el desarrollo de una herramienta de modelado gráfico (CEP Modeler) en la que se pueden modelar consultas CEP de forma gráfica, sencilla y de manera más accesible para el usuario. ABSTRACT Over recent years, more and more companies demand technology for monitoring and analyzing a vast volume of data in real time. In this regard, the CEP technology (Complex Event Processing) has emerged as a novel approach to that end, and its use has increased dramatically in certain domains, such as, management and automation of business processes, finance, monitoring of networks and applications, as well as smart sensor networks as the case study in which we will focus. CEP is based on in the Event Processing Language (EPL). This language can be rather difficult to use for new users. This complexity can be a handicap, and therefore, a problem at the time of extending its use. This project aims to provide a solution to this problem, trying to approach the CEP technology to users through abstraction and modelling techniques. To that end, this project has defined an intuitive and simple domain-specific modelling language for new users through a web tool (CEP Modeler) for graphically modeling CEP queries, in an easier and more accessible way.

Generación automática de la configuración de subestaciones eléctricas según el Estándar IEC 61850 a partir de una herramienta de diseño gráfico

La evolución de las redes eléctricas se dirige hacia lo que se conoce como “Smart Grids” o “Redes Eléctricas Inteligentes”. Estas “Smart Grids” se componen de subestaciones eléctricas, que a su vez se componen de unos dispositivos llamados IEDs (Dispositivos Electrónicos Inteligentes – Intelligent Electronic Devices). El diseño de IEDs se encuentra definido en la norma IEC 61850, que especifica además un Lenguaje de Configuración de Subestaciones (Substation Configuration Language SCL) para la definición de la configuración de subestaciones y sus IEDs. Hoy en día, este estándar internacional no sólo se utiliza para diseñar correctamente IEDs y asegurar su interoperabilidad, sino que también se utiliza para el diseño de otros dispositivos de la red eléctrica, como por ejemplo, medidores inteligentes. Sin embargo, aunque existe una tendencia cada vez mayor del uso de este estándar, la comprensión y el manejo del mismo resulta difícil debido al gran volumen de información que lo compone y del nivel de detalle que utiliza, por lo que su uso para el diseño de IEDs se hace tedioso sin la ayuda de un soporte software. Es por ello que, para facilitar la aplicación del estándar IEC 61850 en el diseño de IEDs se han desarrollado herramientas como “Visual SCL”, “SCL Explorer” o “61850 SCLVisual Design Tool”. En concreto, “61850 SCLVisual Design Tool” es una herramienta gráfica para el modelado de subestaciones electricas, generada mediante el uso de los frameworks Eclipse Modeling Framework (EMF) y Epsilon Generative Modeling Technologies (GMT) y desarrollada por el grupo de investigación SYST de la UPM. El objetivo de este proyecto es añadir una nueva funcionalidad a la herramienta “61850 Visual SCL DesignTool”. Esta nueva funcionalidad consiste en la generación automática de un fichero de configuración de subestaciones eléctricas según el estándar IEC 61850 a partir de de una herramienta de diseño gráfico. Este fichero, se denomina SCD (Substation Configuration Description), y se trata de un fichero XML conforme a un esquema XSD (XML Schema Definition) mediante el que se define el lenguaje de configuración de subestaciones SCL del IEC 61850. Para el desarrollo de este proyecto, es necesario el estudio del lenguaje para la configuración de subestaciones SCL, así como del lenguaje gráfico específico de dominio definido por la herramienta “61850 SCLVisual Design Tool”, la estructura de los ficheros SCD, y finalmente, del lenguaje EGL (Epsilon Generation Language) para la transformación y generación automática de código a partir de modelos EMF. ABSTRACT Electrical networks are evolving to “Smart Grids”. Smart Grids are composed of electrical substations that in turn are composed of devices called IEDs (Intelligent Electronic Devices). The design of IEDs is defined by the IEC 61850 standard, which also specifies a Substation Configuration Languaje (SCL) used to define the configuration of substations and their IEDs. Nowadays, this international standard is not only used to design properly IEDs and guarantee their interoperability, but it is also used to design different electrical network devices, such as, smart meters. However, although the use of this standard is growing, its compression as well as its management, is still difficult due to its large volume of information and its level of detail. As a result, designing IEDs becomes a tedious task without a software support. As a consequence of this, in order to make easier the application of the IEC 61850 standard while designing IEDs, some software tools have been developed, such as: “Visual SCL”, “SCL Explorer” or “61850 SCLVisual Design Tool”. In particular, “61850 SCLVisual Design Tool” is a graphical tool used to make electrical substations models, and developed with the Eclipse Modeling Framework (EMF) and Epsilon Generative Modeling Technologies (GMT) by the research group SYST of the UPM. The aim of this project is to add a new functionality to “61850 Visual SCL DesignTool”. This new functionality consists of the automatic code generation of a substation configuration file according to the IEC 61850 standard. This file is called SCD (Substation Configuration Description), and it is a XML file that follows a XSD (XML Schema Definition) that defines the Substation Configuration Language (SCL) of the IEC 61850. In order to develop this project, it is necessary to study the Substation Configuration Language (SCL), the domain-specific graphical languaje defined by the tool “61850 SCLVisual Design Tool”, the structure of a SCD file, and the Epsilon Generation Language (EGL) used for the automatic code generation from EMF models

Criterios de restauración, intervención y revitalización del patrimonio industrial : la fábrica de gas de San Paolo en Roma

La tesis está centrada en el patrimonio industrial, y trata de ahondar en los temas enunciados en el título, Criterios de restauración, intervención y revitalización del patrimonio industrial (capítulo 1). La fábrica de gas de San Paolo en Roma (capítulo 2): En el primer capítulo, se profundiza en los criterios institucionalizados de actuación en el patrimonio, pero también se habla sobre la singularidad, los valores originales, estratificados en cada construcción y su contexto. Ello resume la intención de la doctoranda al entender la necesidad del análisis de cada obra en modo profundo, concreto y abierto a novedosos acercamientos, antes de su transformación. Al hablar de intervención frente a restauración, se aborda la conocida polémica que plantea el modo de acercarse al legado histórico. Parece necesario enunciar cómo se consideran en el trabajo estos términos. Mientras la restauración (como diría Cesare Brandi, constituye el momento metodológico del reconocimiento de la obra de arte en su consistencia física y en su doble polaridad estética e histórica, en orden a su transmisión al futuro) es una acción más restrictiva, que implica atención suma a la recuperación y conservación de los valores significativos y únicos del bien, en comunicación con un (posible) equipo interdisciplinar; por intervención se entiende aquí un desarrollo más personal y libre por parte del proyectista, en el que se produce un proceso dialéctico entre la preexistencia y el posicionamiento crítico adoptado a la hora de dar un obligado nuevo uso a ese bien. Es decir, el contraste está en el diferente reconocimiento de la materialidad física y sus significados. En torno a la restauración/intervención, se analizan las perspectivas de actuación en España e Italia, contrastando factores como la formación del arquitecto o el entorno laboral-cultural, y utilizando el método analítico comparativo de casos. Habría un tercer concepto: el de revitalización del patrimonio industrial, Éste hace referencia a su transformación, a sus usos más comunes y compatibles, a la redimensión artística que ha experimentado la arquitectura industrial gracias al arte contemporáneo y a su relación con la Modernidad. Por último, se enuncia en el título el caso de estudio, la Fábrica, que ocupa el segundo capítulo. Se trata de un enorme conjunto industrial singular semi abandonado en el confín del centro histórico, cuyo protagonista es un gran gasómetro, que la autora ha tenido la oportunidad de descubrir en su estancia en la Ciudad Eterna. Este barrio constituyó el primer y único sector industrial de la Roma moderna a inicios del siglo XX. Por varias causas históricas y voluntad política, la ciudad prácticamente no tuvo más desarrollo industrial que el área mencionada, donde se implantaron servicios y actividades de gran transformación (Matadero, Mercados Generales, Almacenes Fluviales, Central Montemartini o esta fábrica). A partir de 1920, se empezó a construir un tejido productivo que la llevó de la situación de posguerra al milagro económico. Frente al mito de la Roma monumental o de la Roma cotidiana, aparece el aspecto industrial como mito de la contemporaneidad. Sin embargo, esta ciudad es demasiado antigua como para convertirse en moderna, y su proceso industrial en contraste con su larga historia resulta breve. La inmediata relación de la fase industrial romana con el resultado de este desarrollo viene identificada con las instalaciones señaladas, más significativas por su excepcionalidad que por la duración del momento irrepetible que representan. Su presencia física perdura a pesar de su abandono, aisladas tras su muro perimetral y casi desconocidas. Esto las hace apetecibles para la especulación inmobiliaria y resultan un caso de estudio ideal para realizar un Plan de Intervención Global, en el que determinar una serie de intervenciones necesarias, usos compatibles y directrices para la ordenación del conjunto, coherentes con los conceptos manejados en el primer capítulo. ABSTRACT The thesis focuses on industrial heritage, delving into the topics listed in its title, Criteria for the restoration, intervention and revitalisation of industrial heritage (chapter 1). The San Paolo gas factory in Rome (chapter 2): Chapter one elaborates on the official criteria to take action on heritage, but also deals with the singularity and original values unique to each construction and its context. This underlines the PhD candidate’s intentions by understanding the need for an in-depth and specific analysis, open to novel approaches to each site before its transformation. When speaking of intervention, as opposed to restoration, we address the well-known controversy that stems from the different ways of approaching historical heritage. It seems necessary to explain how these terms are interpreted in this piece of work. Whereas restoration (as Cesare Brandi would say, constitutes the methodological moment in which the work of art is appreciated in its material form and in its historical and aesthetic duality, with a view to transmitting it to the future) is a more restrictive action, demanding the utmost attention to the recovery and preservation of the piece of heritage’s defining and exceptional values, (ideally) in communication with a interdisciplinary team. By intervention, we hereby understand a more personal and free procedure carried out by the designer in which a dialectical process takes place between preexistence and the critical stance taken in order to give that good a much needed new use. That is, contrast lies in the different recognition of physical materiality and its meanings. As for the restoration/intervention dilemma, the different courses of action followed in Spain and Italy will be analysed, contrasting the architects’ training, the labourcultural environment and taking an analytical comparative case study method. There is a third concept: the revitalisation of industrial heritage, which refers to its transformation, its most common and compatible uses, the artistic redimensioning that industrial architecture has experienced thanks to contemporary art, and its relationship with Modernity. Lastly, the title refers to the case study, i.e., the analysis of the Factory, which makes up chapter 2. It is an enormous and semiabandoned peerless industrial complex on the edge of the historical city centre whose main landmark is a huge gasometer, which the author had the chance to discover during her stay in the Eternal City. This district made up the first and only industrial sector in the modern Rome of the early 20th century. Due to a number of reasons, both historical and political, barely no further industrial development took place in the city beyond the mentioned area, where processing services and activities were set up (Slaughterhouse, General Markets, Riverside Warehouses, Montemartini Power Plant or the said factory). In the 1920s began the construction of the productive fabric that raised the city from its postwar plight to the economic miracle. As opposed to the myth of monumental Rome and daily-life Rome, this industrial Rome rose as contemporaneity’s myth. However, this city is too ancient to become modern, and its industrial process strikes us as brief when confronted with its long history. The close relationship between Rome’s industrial stage and the consequences of this development is best represented by the aforementioned facilities, more relevant due to their uniqueness than the length of the once-in-a-lifetime moment they represent. Their physical presence lives on despite abandonment and isolation, as they stand vastly unknown behind their perimeter walls. This renders them appealing to property speculators, becoming an ideal case study for the creation of a Global Intervention Plan in which to determine a series of necessary actions, compatible uses and directives, all of them consistent with the concepts dealt with in chapter 1. RIASSUNTO La tesi è focalizzata sul patrimonio industriale e cerca di approfondire le tematiche contenute nel titolo, Criteri di restauro, intervento e rivitalizzazione del patrimonio industriale (capitolo 1). La Fabbrica del gas di San Paolo a Roma (capitolo 2): Nel primo capitolo vengono presi in esame i criteri istituzionalizzati di attuazione nel patrimonio, ma si parla anche della unicità, i valori originali, stratificati in ogni costruzione ed il suo contesto. Questo riassume l’intenzione della dottoranda di capire la necessità dell’analisi di ogni opera in modo profondo, concreto e aperto a nuovi approcci, prima della sua trasformazione. Nel parlare di intervento versus restauro, si affronta la famosa polemica che considera il modo di avicinarsi all’eredità storica. Appare necessario precisare come questi termini sono considerati in questo lavoro. Mentre il restauro (come direbbe Cesare Brandi, costituisce il momento metodologico del riconoscimento dell’opera d’arte nella sua consistenza fisica e nella duplice polarità estetica e storica, in vista della sua trasmissione nel futuro) è un’azione più restrittiva, e quindi implica massima attenzione al recupero e conservazione dei valori significativi ed unici del bene, in comunicazione con una (eventuale) squadra interdisciplinare; per intervento si intende qui un processo più personale e libero del progettista, nel quale si produce uno sviluppo dialettico tra la preesistenza e la posizione critica adottata quando si deve dare un nuovo uso di quel bene. Vale a dire che il contrasto risiede nel diverso riconoscimento della materialità fisica e i loro significati. Intorno al restauro / intervento, si analizzano le prospettive di attuazione in Spagna e Italia, contrastando diversi fattori come la formazione dell’architetto o l’ambiente lavorativo-culturale, ed impiegando il metodo analitico comparativo dei casi. Ci sarebbe un terzo concetto: quello della rivitalizzazione del patrimonio industriale. Questo fa riferimento alla sua trasformazione, ai suoi usi più comuni e compatibili, al ridimensionamento artistico vissuto dall’archeologia industriale grazie all’arte contemporanea ed al suo rapporto con la Modernità. In ultimo, si enuncia nel titolo il caso di studio, ovvero l’analisi della Fabbrica, che occupa il secondo capitolo. Si tratta di un enorme complesso industriale semi-abbandonato al confine con il centro storico, il cui protagonista è un grande gasometro, che l’autrice ha avuto modo di scoprire durante il suo soggiorno nella Città Eterna. Questo quartiere costituì il primo e unico moderno settore industriale della Roma moderna all’inizio del Novecento. Per diverse ragioni storiche e volontà politiche, la città praticamente non ha subito ulteriore sviluppo industriale oltre l’area citata, dove si impiantarono servizi e attività di grande trasformazione (Mattatoio, Mercati Generali, Magazzini Generali, Centrale Montemartini o questa fabbrica). Dal 1920, si inizió a costruire un tessuto prodottivo che portò la città dalla situazione post-bellica al miracolo economico. Di fronte al mito della Roma monumentale o della Roma quotidiana, appare questa Roma industriale come mito della contemporaneità. Tuttavia, questa città è troppo antica per diventare moderna, ed il suo processo industriale in contrasto con la sua lunga storia risulta breve. Il rapporto diretto della fase industriale romana con il risultato di questo sviluppo viene identificato con gli impianti segnalati, più significativi per la loro unicità che per la durata del momento irripetibile che rappresentano. La loro presenza fisica perdura nonostante l’abbandono, isolati dietro il loro muro di cinta e quasi sconosciuti. Questo li rende auspicabile per la speculazione immobiliare e sono un caso ideale per eseguire un Piano di Intervento Globale, che determini una serie di interventi necessari, usi compatibili e linee guida per la pianificazione del tutto, in linea con i concetti utilizzati nel primo capitolo.

Syndapin I, a Synaptic Dynamin-binding Protein that Associates with the Neural Wiskott-Aldrich Syndrome Protein

The GTPase dynamin has been clearly implicated in clathrin-mediated endocytosis of synaptic vesicle membranes at the presynaptic nerve terminal. Here we describe a novel 52-kDa protein in rat brain that binds the proline-rich C terminus of dynamin. Syndapin I (synaptic, dynamin-associated protein I) is highly enriched in brain where it exists in a high molecular weight complex. Syndapin I can be involved in multiple protein–protein interactions via a src homology 3 (SH3) domain at the C terminus and two predicted coiled-coil stretches. Coprecipitation studies and blot overlay analyses revealed that syndapin I binds the brain-specific proteins dynamin I, synaptojanin, and synapsin I via an SH3 domain-specific interaction. Coimmunoprecipitation of dynamin I with antibodies recognizing syndapin I and colocalization of syndapin I with dynamin I at vesicular structures in primary neurons indicate that syndapin I associates with dynamin I in vivo and may play a role in synaptic vesicle endocytosis. Furthermore, syndapin I associates with the neural Wiskott-Aldrich syndrome protein, an actin-depolymerizing protein that regulates cytoskeletal rearrangement. These characteristics of syndapin I suggest a molecular link between cytoskeletal dynamics and synaptic vesicle recycling in the nerve terminal.

A Novel Tetanus Neurotoxin-insensitive Vesicle-associated Membrane Protein in SNARE Complexes of the Apical Plasma Membrane of Epithelial Cells

The importance of soluble N-ethyl maleimide (NEM)-sensitive fusion protein (NSF) attachment protein (SNAP) receptors (SNAREs) in synaptic vesicle exocytosis is well established because it has been demonstrated that clostridial neurotoxins (NTs) proteolyze the vesicle SNAREs (v-SNAREs) vesicle-associated membrane protein (VAMP)/brevins and their partners, the target SNAREs (t-SNAREs) syntaxin 1 and SNAP25. Yet, several exocytotic events, including apical exocytosis in epithelial cells, are insensitive to numerous clostridial NTs, suggesting the presence of SNARE-independent mechanisms of exocytosis. In this study we found that syntaxin 3, SNAP23, and a newly identified VAMP/brevin, tetanus neurotoxin (TeNT)-insensitive VAMP (TI-VAMP), are insensitive to clostridial NTs. In epithelial cells, TI-VAMP–containing vesicles were concentrated in the apical domain, and the protein was detected at the apical plasma membrane by immunogold labeling on ultrathin cryosections. Syntaxin 3 and SNAP23 were codistributed at the apical plasma membrane where they formed NEM-dependent SNARE complexes with TI-VAMP and cellubrevin. We suggest that TI-VAMP, SNAP23, and syntaxin 3 can participate in exocytotic processes at the apical plasma membrane of epithelial cells and, more generally, domain-specific exocytosis in clostridial NT-resistant pathways.

Apical sorting of a voltage- and Ca2+-activated K+ channel α-subunit in Madin-Darby canine kidney cells is independent of N-glycosylation

The voltage- and Ca2+-activated K+ (KV,Ca) channel is expressed in a variety of polarized epithelial cells seemingly displaying a tissue-dependent apical-to-basolateral regionalization, as revealed by electrophysiology. Using domain-specific biotinylation and immunofluorescence we show that the human channel KV,Ca α-subunit (human Slowpoke channel, hSlo) is predominantly found in the apical plasma membrane domain of permanently transfected Madin-Darby canine kidney cells. Both the wild-type and a mutant hSlo protein lacking its only potential N-glycosylation site were efficiently transported to the cell surface and concentrated in the apical domain even when they were overexpressed to levels 200- to 300-fold higher than the density of intrinsic Slo channels. Furthermore, tunicamycin treatment did not prevent apical segregation of hSlo, indicating that endogenous glycosylated proteins (e.g., KV,Ca β-subunits) were not required. hSlo seems to display properties for lipid-raft targeting, as judged by its buoyant distribution in sucrose gradients after extraction with either detergent or sodium carbonate. The evidence indicates that the hSlo protein possesses intrinsic information for transport to the apical cell surface through a mechanism that may involve association with lipid rafts and that is independent of glycosylation of the channel itself or an associated protein. Thus, this particular polytopic model protein shows that glycosylation-independent apical pathways exist for endogenous membrane proteins in Madin-Darby canine kidney cells.