325 resultados para Ammaker, Tommy
Resumo:
Compromised skin integrity of farmed Atlantic salmon, commonly occurring under low temperature and stressful conditions, has major impacts on animal welfare and economic productivity. Even fish with minimal scale loss and minor wounds can suffer from secondary infections, causing downgrading and mortalities. Wound healing is a complex process, where water temperature and nutrition play key roles. In this study, Atlantic salmon (260 g) were held at different water temperatures (4 or 12 °C) and fed three different diets for 10 weeks, before artificial wounds were inflicted and the wound healing process monitored for 2 weeks. The fish were fed either a control diet, a diet supplemented with zinc (Zn) or a diet containing a combination of functional ingredients in addition to Zn. The effect of diet was assessed through subjective and quantitative skin histology and the transcription of skin-associated chemokines. Histology confirmed that wound healing was faster at 12 °C. The epidermis was more organised, and image analyses of digitised skin slides showed that fish fed diets with added Zn had a significantly larger area of the epidermis covered by mucous cells in the deeper layers after 2 weeks, representing more advanced healing progression. Constitutive levels of the newly described chemokines, herein named CK 11A, B and C, confirmed their preferential expression in skin compared to other tissues. Contrasting modulation profiles at 4 and 12 °C were seen for all three chemokines during the wound healing time course, while the Zn-supplemented diets significantly increased the expression of CK 11A and B during the first 24 h of the healing phase.
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The data describe the flows of nitrogen between different pools and economic sectors within Denmark. The data are stored in an Excel spreadsheet that is divided into a number of worksheets. The National worksheet contains the national flows of nitrogen for the years 1990 to 2010 (note that for some flows, the data series is not complete for all years). These data underlie the national nitrogen flow figures in the main text of the paper. The remaining worksheets contain the data that underlie the figures presented in the detailed description of nitrogen flows between pools/sectors, that is in the Supplementary Material associated with the paper.
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© 2015 Wiley Periodicals, Inc.
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We report here the characterization of gp27 (hp24γ3), a glycoprotein of the p24 family of small and abundant transmembrane proteins of the secretory pathway. Immunoelectron and confocal scanning microscopy show that at steady state, gp27 localizes to the cis side of the Golgi apparatus. In addition, some gp27 was detected in COPI- and COPII-coated structures throughout the cytoplasm. This indicated cycling that was confirmed in three ways. First, 15°C temperature treatment resulted in accumulation of gp27 in pre-Golgi structures colocalizing with anterograde cargo. Second, treatment with brefeldin A caused gp27 to relocate into peripheral structures positive for both KDEL receptor and COPII. Third, microinjection of a dominant negative mutant of Sar1p trapped gp27 in the endoplasmic reticulum (ER) by blocking ER export. Together, this shows that gp27 cycles extensively in the early secretory pathway. Immunoprecipitation and coexpression studies further revealed that a significant fraction of gp27 existed in a hetero-oligomeric complex. Three members of the p24 family, GMP25 (hp24α2), p24 (hp24β1), and p23 (hp24δ1), coprecipitated in what appeared to be stochiometric amounts. This heterocomplex was specific. Immunoprecipitation of p26 (hp24γ4) failed to coprecipitate GMP25, p24, or p23. Also, very little p26 was found coprecipitating with gp27. A functional requirement for complex formation was suggested at the level of ER export. Transiently expressed gp27 failed to leave the ER unless other p24 family proteins were coexpressed. Comparison of attached oligosaccharides showed that gp27 and GMP25 recycled differentially. Only a very minor portion of GMP25 displayed complex oligosaccharides. In contrast, all of gp27 showed modifications by medial and trans enzymes at steady state. We conclude from these data that a portion of gp27 exists as hetero-oligomeric complexes with GMP25, p24, and p23 and that these complexes are in dynamic equilibrium with individual p24 proteins to allow for differential recycling and distributions.
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Caspase-3 knockout mice exhibit thickening of the internal granule cell layer of the cerebellum. Concurrently, it has been shown that intracerebral injection of pituitary adenylate cyclase-activating polypeptide (PACAP) induces a transient increase of the thickness of the cerebellar cortex. In the present study, we have investigated the possible effect of PACAP on caspase activity in cultured cerebellar granule cells from 8-day-old rat. Incubation of granule neurons with PACAP for 24 h promoted cell survival and prevented DNA fragmentation. Exposure of cerebellar granule cells to the specific caspase-3 inhibitor N-benzyloxycarbonyl-Asp-Glu-Val-Asp fluoromethylketone (Z-DEVD-FMK) for 24 h markedly enhanced cell survival and inhibited apoptotic cell death. Time-course studies revealed that PACAP causes a prolonged inhibition of caspase-3 activity without affecting caspase-1. Administration of graded concentrations of PACAP for 3 h induced a dose-dependent inhibition of caspase-3 activity. Incubation of granule cells with both dibutyryl-cAMP (dbcAMP) and phorbol 12-myristate 13-acetate (PMA) mimicked the inhibitory effect of PACAP on caspase-3. Cotreatment of cultured neurons with the protein kinase A inhibitor H89 and the protein kinase C inhibitor chelerythrine abrogated the effect of PACAP on caspase-3 activity. In contrast, the ERK kinase inhibitor U0126 did not affect the action of PACAP on caspase-3 activity. These data demonstrate that PACAP prevents cerebellar granule neurons from apoptotic cell death through a protein kinase A- and protein kinase C-dependent inhibition of caspase-3 activity.
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The merozoite stage of the malaria parasite that infects erythrocytes and causes the symptoms of the disease is initially formed inside host hepatocytes. However, the mechanism by which hepatic merozoites reach blood vessels (sinusoids) in the liver and escape the host immune system before invading erythrocytes remains unknown. Here, we show that parasites induce the death and the detachment of their host hepatocytes, followed by the budding of parasite-filled vesicles (merosomes) into the sinusoid lumen. Parasites simultaneously inhibit the exposure of phosphatidylserine on the outer leaflet of host plasma membranes, which act as "eat me" signals to phagocytes. Thus, the hepatocyte-derived merosomes appear to ensure both the migration of parasites into the bloodstream and their protection from host immunity.
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Back Row: Todd Jager, Kevin Lynch, Paul Schmidt, Mike Gittleson, Rick Clark, Vance Bedford, Brady Hoke, Jim Herrmann, Mike DeBord, Fred Jackson, Bobby Morrison, Stan Parrish, Erik Campbell, Terry Malone, Harold Goodwin, Scott Draper, Jon Falk, Phil Bromley, Mike Elston, Chris Kurpeikis
8th Row: Eddie Davis, Eric Dean, Eddie Magnus, Ethan Perkins, Toni Grant, Brodie Killian, Jake Malacos, Jeremy Miller, Gary Rose, Rudy Smith, Jamie Young, Bob Bland, Rick Brandt, Steve Connelly, Kelly Cox, Peter Clifford, Matt Hamilton, Scott Loeffler
7th Row: Patrick McCall, James Whitley, DeWayne Patmon, Anthony Jordan, Eric Brackins, Ray Jackson, Adam Adkins, Bob Fraumann, Shawn Thompson, Todd Mossa, Bill Seymour, Eric Rosel, Demetrius Smith, Anthony Thomas, William Peterson, Johathan West, P.J. Cwayna
6th Row: David Downs, Chad Henman, Jeff Del Verne, Ryan Parini, Mark Bergin, Eric Wilson, Eric Warner, Jake Frysinger, Kurt Anderson, Maurice Williams, Ben Mast, Jason Brooks, Andy Sechler, Dan Williams, Chris Roth, Matt Johnson
5th Row: LeAundre Brown, Ian Gold, Grady Brooks, Cory Sargent, Kevin Brandt, Steve Hutchinson, Jason Kapsner, Jeff Backus, Paul Tannous, Chad Carpenter, Jerry Johnson, Tommy Hendricks, Dhani Jones, Marcus Knight, Aaron Wright
4th Row: J.R. Ford, Kenneth Jackson, Tate Schanski, Jason Cummings, Rob Renes, Jeff Holtry, Pat Kratus, Lance Ostram, Jason Clyne, Jared Chandler, Kevin Bryant, Jeff Smokevitch, Chad Stock, Brandon Kornblue, Manus Edwards
3rd Row: Clarence Williams, DaydrionTaylor, Charles Woodson, DiAllo Johnson, James Hall, Steve Frazier, Tom Brady, Jeff Potts, Chris Ziemann, Tai Streets, Aaron Shea, Josh Williams, Jason Vinson, Brent Washington, Darren Petterson
2nd Row: Jay Feely, Todd Brooks, Marcus Ray, Kraig Baker, Sam Sword, Nate Miller, Juaquin Feazell, Mark Campbell, JerameTuman, Clint Copenhaver, Noah Parker, Mike Singletary, Scott Parachek, Andre Weathers, Russell Shaw
Front Row: Dr. Gerald O'Connor, Brian Griese, Colby Keefer, Eric Mayes, Rob Swett, Zach Adami, Glen Steele, Head Coach Lloyd Carr, Ben Huff, Chris Howard, Chris Floyd, David Crispin, Jon Jansen, Scott Dreisbach, Terrence Quinn
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Back Row: Paul Schmidt, Mike Gittleson, Rick Clark, Vance Bedford, Brady Hoke, Jim Herrmann, Mike DeBord, Fred Jackson, Bobby Morrison, Stan Parrish, Erik Campbell, Terry Malone, Scot Loeffler, Jon Falk, Phil Bromley, Mike Elston
8th Row: Tim Murphy, Dave Dean, Dr. Edward Wojtys, Dr. C. Daniel Hendrickson, Danielle Tiernan, Steve Connelly, Dwight Mosely, Scott Panique, Kirk Moundros, Tad Van Pelt, Mike Sajdak, Pete Clifford, Rob Abin, Rick Brandt, Mark Ouimet, Kelly Cox, Eric Dean, Buster Stanley, Jim Schneider
7th Row: Daydrion Taylor, Todd Howard, Walter Cross, Evan Coleman, Julius Curry, Justin Fargas, Hayden Epstein, Larry Foote, Shawn Lazarus, Victor Hobson, Dave Armstrong, Deitan Dubuc, Jonathan Goodwin, John Wood, Dennis Baker, Jason Ptak, Kyle Froelich, Paul Tannous
6th Row: Aaron Richards, Cyle Young, P.J. Cwayna, Jeremy Miller, Michael Manning, Jake Malacos, Brodie Killian, Gary Rose, Rudy Smith, Joe Denay, Bennie Joppru, Dan Rumishek, Dave Petruziello, Drew Henson, Dave Terrell, Marquise Walker, Cato June
5th Row: Patrick McCall, James Whitley, William Peterson, Anthony Thomas, Ray Jackson, Bill Seymour, Shawn Thompson, Kurt Anderson, Jason Brooks, Ben Mast, Adam Adkins, Todd Mossa, Bob Fraumann, Eric Brackins, Eric Rosel, DeWayne Patmon, Anthony Jordan
4th Row: Manus Edwards, Chris Roth, Dan Williams, LeAundre Brown, Eric Wilson, Chad Carpenter, Ian Gold, Marcus Knight, Eric Warner, Maurice Williams, Jake Frysinger, Grady Brooks, Cory Sargent, Ryan Parini, Andy Sechler, Jeff Del Verne
3rd Row: Brent Washington, Kevin Bryant, Jeff Smokevich, Mark Bergin, Kenneth Jackson, Jeff Holtry, David Brandt, Steve Hutchinson, Jeff Backus, Jason Kapsner, Tommy Hendricks, Dhani Jones, Jared Chandler, Tate Schanski, Brandon Kornblue, Matt Johnson
2nd Row: Jay Feely, Darren Petterson, Jason Vinson, Noah Parker, Aaron Shea, James Hall, Steve Frazier, Chris Ziemann, Jeff Potts, Tom Brady, Josh Williams, Patrick Kratus, DiAllo Johnson, Rob Renes, Kraig Baker
Front Row: Head Coach Lloyd Carr, Marcus Ray, Andre Weathers, Nate Miller, Sam Sword, Juaquin Feazell, Mark Campbell, Jon Jansen, Jerame Tuman, Clint Copenhaver, Tai Streets, Scott Dreisbach, Chris Singletary, Clarence Williams
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Back Row: Paul Schmidt, Mike Gittleson, Mike Elston, Teryl Austin, Brady Hoke, Jim Herrmann, Mike DeBord, Fred Jackson, Bobby Morrison, Stan Parrish, Erik Campbell, Terry Malone, Scot Loeffler, Jon Falk, Scott Draper, Phil Bromley, Jim Schneider
8th Row: Tim Murphy, Dr. Edward Wojtys, Dr. C. Daniel Hendrickson, Kevin Undeen, Mark Borgman, Brian Smalls, Michael Kaselitz, Joe Ghannam, Tommy Huff, Dave Eklund, Rick Brandt, Bob Bland, Mark Ouimet, Kelly Cox, Dennis Coyle, Zach Adami
7th Row: Jason Clyne, Brandon Williams, Greg Brooks, Shantee Orr, Jeremy LeSueur, Carl Biggs, Dave Pearson, Ronald Bellamy, Tyrece Butler, John Navarre, Andy Mignery, Andy Brown, Grant Bowman, Courtney Morgan, Phil Brabbs*, Kyle Blerlein, Chris Roth
6th Row: P.J. Cwayna, TommyJones, Tad Van Pelt, Dwight Mosley, Scott Panique, Stephen Baker, Blake Nasif, Joe Sgroi, Tony Pape, Demeterius Soloman, Norman Boebert, John Spytek, Phil Brackins, B.J. Askew, Charles Drake, Brent Cummings, Ryan Beard, Jon Shaw
5th Row: Aaron Richards, Jason Ptak, Todd Howard, Walter Cross, Julius Curry, Justin Fargas, Bennie Joppru, Dan Rumishek, Dave Petruziello, Shawn Lazarus, Victor Hobson, Dave Armstrong, Deitan Dubuc, Cato June, John Wood, Kyle Froelich, Kirk Moundros
4th Row: Mark Bergin, Cyle Young, Bob Fraumann, Kurt Anderson, Todd Mossa, Rudy Smith, Evan Coleman, Hayden Epstein, Larry Foote, Joe Denay, Drew Henson, Dave Terrell, Marquise Walker, Gary Rose, Michael Manning, Jeremy Miller
3rd Row: Matt Johnson, Ryan Parini, James Whitley, Bill Seymour, Anthony Thomas, Shawn Thompson, Adam Adkins, Jake Frysinger, Ben Mast, Eric Brackins, Eric Rosel, DeWayne Patmon, Dan Williams, Cory Sargent, Brandon Kornblue
2nd Row: Tate Schanski, Jeff Smokevitch, Kevin Bryant, Eric Wilson, Grady Brooks, David Brandt, Steve Frazier, Steve Hutchinson, Jeff Backus, Jason Kapsner, Andy Sechler, Eric Warner, Ken Jackson, Jeff Del Verne
Front Row: Chris Ziemann, Josh Williams, Tom Brady, Patrick Kratus, DiAllo Johnson, Rob Renes, Head Coach Lloyd Carr, Dhani Jones, Ian Gold, Marcus Knight, Tommy Hendricks, Aaron Shea, James Hall
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Matthew Johnson (49), Brandon Kornblue (2), Dhani Jones (55), Eric Brackins (41), James Hall (56), Tom Brady (10), ? (27), Tommy Hendricks (41), Aaron Shea (36), Andy Sechler (46)
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Back Row (standing): Coach Tommy Amaker, director of basketball operations Kirsten Green, strength coach Jim Plocki, asst. coach Charles E. Ramsey, Dommanic Ingerson, Bernard Robinson Jr., Chuck Bailey, Josh Moore, Ron Garber, Colin Dill, LaVell Blanchard, Herb Gibson, asst. coach Billy Schmidt, equip. mngr. Bob Bland, asst. coach Chuck Swenson.
Front Row: admin. asst. Steve Grialou, mngr. Alan Hibino, Avery Queen, Marcus Bennett, captain Rotolu Adebiyi, captain Chris Young, captain Leon Jones, Gavin Groninger, Mike Gotfredson, mngr. Lucien St. Gerard, trainer Eric Broekhuizen.
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Back Row:: academic advisor Angie Heath, head coach Tommy Amaker, director of basketball operations Kirsten Green, asst. coach Billy Schmidt, asst. coach Charles E. Ramsey, Lester Abram, Chuck Bailey, J.C. Mathis, Graham Brown, Chris Hunter, Amadou Ba, Colin Dill, Bernard Robinson Jr., admin. asst. Andy Moore, strength coach Jim Plocki, asst. coach Chuck Swenson, equip. mngr. Bob Bland.
Front Row: trainer Eric Broekhuizen, sr. mngr. Michael Oh, Sherrod Harrell, Dommanic Ingerson, Gavin Groninger, LaVell Blanchard, Rotolu Adebiyi, Daniel Horton, Avery Queen, sr. mngr. Jesse Grasty, sr. mngr. Lucien St. Gerard.
Resumo:
Back Row: coach Tommy Amaker, director of basketball operations Kirsten Green, asst. coach Chuck Swenson, asst. coach Charles E. Ramsey, Lester Abram, Brent Petway, Amadou Ba, Chris Hunter, Courtney Sims, Graham Brown, John Andrews, asst. coach Andrew Moore, strength coach Jim Plocki, academic advisor Support Angie Heath, equip. mngr. Bob Bland.
Front Row: trainer Eric Broekhuizen, mngr. Michael Oh, Sherrod Harrell, Daniel Horton, Colin Dill, Bernard Robinson Jr., J.C. Mathis, Dion Harris, Dani Wohl, Manager Matt Duprey, admin. asst. Lucien St. Gerard.
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Back Row: head coach Tommy Amaker, director of basketball operations Kirsten Green, asst. coach Chuck Swenson, asst. coach Charles Ramsey, John Andrews, Brent Petway, Courtney Sims, Chris Hunter, Amadou Ba, Graham Brown, Ronald Coleman, asst. coach Andrew More academic advisor Angie Beck, strength coach Jim Plocki, equip. mngr. Bob Bland.
Front Row: administrative asst. Lucien St. Gerard, Dani Wohl, Dion Harris, Sherrod Harrell, J.C. Mathis, Lester Abram, Daniel Bell, sr. mngr. Matt Duprey.
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Back Row (standing): head coach Tommy Amaker, asst. coach Chuck Swenson, asst. coach Mike Jackson, director of basketball operations Kirsten Green, asst. coach Andrew Moore, Kendric Price, Phillip DeVries, Amadou Ba, Chris Hunter, Courtney Sims, Graham Brown, Brent Petway and Ron Coleman, admin. asst. Dave Pilipovich, staff asst. Matt Duprey, equip. mngr. Bob Bland, trainer John DoRosario, strength coach Jim Plocki
Front Row: mngr. Jeff Rapp, Jerret Smith, Dion Harris, Sherrod Harrell, Daniel Horton, Lester Abram, Alex Brzozowicz, Jevohn Shepherd, Hayes Grooms, mngr. Nathan Zandt
