中国白裤瑶永生细胞库的建立

目的　建立和保存白裤瑶B 淋巴母细胞永生细胞株。方法　采用EB 病毒转化外周血B 淋巴细胞,同 时加环孢菌素A 法。结果　成功地建立了含有44 株永生细胞的白裤瑶永生细胞库。供血者身体健康,三代均为 白裤瑶。结论　建立永生细胞库,可永久保存白裤瑶特有的基因组,为民族起源、演进和遗传多样性的研究提供细 胞资源。

Precracked reinforced concrete T-Beams repaired in shear with bonded carbon Fiber-Reinforced Polymer sheets

This study investigates the structural behavior of precracked reinforced concrete (RC) T-beams strengthened in shear with externally bonded carbon fiber-reinforced polymer (CFRP) sheets. It reports on seven tests on unstrengthened and strengthened RC T-beams, identifying the influence of load history, beam depth, and percentage of longitudinal steel reinforcement on the structural behavior. The experimental results indicate that the contributions of the external CFRP sheets to the shear force capacity can be significant and depend on most of the investigated variables. This study also investigates the accuracy of the prediction of the fiber-reinforced polymer (FRP) contribution in ACI 440.2R-08, UK Concrete Society TR55, and fib Bulletin 14 design guidelines for shear strengthening. A comparison of predicted values with experimental results indicates that the guidelines can overestimate the shear contribution of the externally bonded FRP system. © 2012, American Concrete Institute.

The influence of turbine stator well coolant flow rate and passage configuration on cooling effectiveness

Market competitiveness for aero engine power plant dictates that improvements in engine performance and reliability are guaranteed a priori by manufacturers. The requirement to accurately predict the life of engine components makes exacting demands of the internal air system, which must provide effective cooling over the engine duty cycle with the minimum consumption of compressor section air. Tests have been conducted at the University of Sussex using a turbine test facility which comprises a two stage turbine with an individual stage pressure ratio of 1.7:1. Main annulus air is supplied by an adapted Rolls-Royce Dart compressor at up to 440 K and 4.8 kg s-1. Cooling flow rates ranging from 0.71 to 1.46 Cw, ent, a disc entrainment parameter, have been used to allow ingress or egress dominated stator well flow conditions. The mechanical design of the test section allows internal cooling geometry to be rapidly re-configured, allowing the effect of jet momentum and coolant trajectory to be investigated. An important facet to this investigation is the use of CFD to model and analyse the flow structures associated with the cavity conditions tested, as well as to inform the design of cooling path geometry. This paper reports on the effectiveness of stator well coolant flow rate and delivery configurations using experimental data and also CFD analysis to better quantify the effect of stator well flow distribution on component temperatures. Copyright © 2011 by Rolls-Royce plc.

The development of a scanning strategy for the manufacture of porous biomaterials by selective laser melting.

Porous structures are used in orthopaedics to promote biological fixation between metal implant and host bone. In order to achieve rapid and high volumes of bone ingrowth the structures must be manufactured from a biocompatible material and possess high interconnected porosities, pore sizes between 100 and 700 microm and mechanical strengths that withstand the anticipated biomechanical loads. The challenge is to develop a manufacturing process that can cost effectively produce structures that meet these requirements. The research presented in this paper describes the development of a 'beam overlap' technique for manufacturing porous structures in commercially pure titanium using the Selective Laser Melting (SLM) rapid manufacturing technique. A candidate bone ingrowth structure (71% porosity, 440 microm mean pore diameter and 70 MPa compression strength) was produced and used to manufacture a final shape orthopaedic component. These results suggest that SLM beam overlap is a promising technique for manufacturing final shape functional bone ingrowth materials.

复合垂直流人工湿地氧化还原特征及不同功能区净化作用研究

研究了复合垂直流人工湿地系统氧化还原电位(Eh)的时空变化规律及不同功能区中污染物的净化效果。结果表明:湿地系统下行流池表层、两池底层、上行流池表层Eh的变化范围分别为402~585、-87~-130、308~432mV,沿水流方向依次形成了好氧A区/缺氧、厌氧区/好氧B区3个功能区。好氧A区是污染物去除的主要区域,BOD5、CODCr、NH4+-N的去除率分别为43.0%、48.4%、54.1%,特别是NH4+-N去除率占总去除率的79%;缺氧、厌氧区主要是进行反硝化反应和有机物的厌氧分解;好氧B区则主

鳜鱼头肾的组织发生及成鱼头肾B淋巴细胞的分布

通过整体连续切片,研究了鳜鱼不同发育时期的头肾结构,并利用原位PCR方法检测了B淋巴细胞在鳜鱼头肾中的分布。在孵化后第1d观察到了肾组织,主要由肾小管组成。尔后头肾的发育经历了三个结构和功能的转变。第一个阶段为孵化后第1d到第7d,头肾作为滤过性器官存在,由肾小管及少量淋巴细胞组成。第二个阶段从第8d到第36d,是一个功能混合型阶段,头肾中既有肾小管,又有造血组织;随时间推移,肾小管数量减少,淋巴细胞数量剧增。紧接着进入第三个阶段:肾小管完全消失,头肾中开始出现大量的嗜铬细胞,头肾作为淋巴-肾上腺组织而存

As污染区农民头发中As含量与环境中As含量的关系

为研究As污染区农民头发中As含量与环境中As含量的关系,采用分层整群随机抽样方式,抽取湖北省气型和污灌型As污染区6个村农民头发样660份,同时采集这6个村的饮用水、土壤、食物(大米、小麦、蔬菜、鸡蛋、鸡肉、猪肉)样品,用原子荧光光谱法测定As含量.研究结果表明,两种类型As污染区农民头发中As含量污染区明显高于对照区,农民头发中As含量有随着区域土壤和食品中As含量增高而相应增高的趋势.对不同年龄组研究表明,高龄组头发中砷含量最高,反映出人体慢性暴露As蓄积的现象,而污染区人群男女头发中As含量差异不