The complete mitochondrial genome of the ridgetail white prawn Exopalaemon carinicauda Holthuis, 1950 (Crustacean: Decapoda: Palaemonidae) revealed a novel rearrangement of tRNA genes

The complete mitochondrial (mt) DNA sequence was determined for a ridgetail white prawn, Exopalaemon carinicauda Holthuis, 1950 (Crustacea: Decopoda: Palaemonidae). The mt genome is 15,730 bp in length, encoding a standard set of 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, which is typical for metazoans. The majority-strand consists of 33.6% A, 23.0% C, 13.4% G, and 30.0% T bases (AT skew = 0.057: GC skew = -0.264). A total of 1045 bp of non-coding nucleotides were observed in 16 intergenic regions,,including a major A+ T rich (79.7%) noncoding region (886 bp). A novel translocation of tRNA(Pro) and tRNA(Thr) was found when comparing this genome with the pancrustacean ground pattern indicating that gene order is not conserved among caridean mitochondria. Furthermore, the rate of Ka/Ks in 13 protein-coding genes between three caridean species is Much less than 1, which indicates a strong Purifying selection within this group. To investigate the phylogenetic relationship within Malacostraca, phylogenetic trees based oil Currently available malacostracan complete mitochondrial sequences were built with the maximum likelihood and Bayesian models. All analyses based oil nucleotide and amino acid data strongly support the monophyly of Decapoda. The Penaeidae, Reptantia, Caridea, and Meiura clades were also recovered as monophyletic groups with Strong Statistical Support. However, the phylogenetic relationships within Pleocyemata are unstable, as represented by the inclusion or exclusion of Caridea. (C) 2009 Elsevier B.V. All rights reserved.

甘肃黄芪对多柔比星心肌病大鼠心肌组织部分基因表达的影响

目的 比较正常大鼠和多柔比星心肌病大鼠心肌组织部分基因表达谱的差异,探讨甘肃黄芪对上述基因表达谱的影响.方法 30只Wistar大鼠随机分3组:对照组即生理盐水组(n=10),多柔比星组(n=10)和黄芪+多柔比星组(n=10).分别从3组心肌组织中抽提总RNA,用Cy3,Cy5荧光标记,经逆转录合成动物来源的eDNA探针;eDNA探针与4000点基因表达谱芯片杂交,结果由软件分析表达信号.结果 共有923条基因出现差异表达,其中586条基因表达下调,337条基因表达上调.凋亡相关基因表达在多柔比星心肌病时上调;氧化和能量代谢相关基因的表达在多柔比星心肌病时下调.6个在多柔比星组上调的基因在黄芪+多柔比星组下调,8个在多柔比星组下调的基因在黄芪+多柔比星组上调,3个在多柔比星组上调的基因在黄芪+多柔比星组表达进一步增强.结论 凋亡和能量代谢障碍及免疫因素在多柔比星心肌病的发病机制中参与作用,甘肃黄芪对多柔比星诱导的心肌病大鼠的心脏保护作用是通过多基因多途径调节相关基因表达而实现的.

艾虎对不同猎物的选择性

在室内条件下,将高原鼢鼠（Myospalax baileyi）、高原鼠兔（Ochotona curzoniae）和根田鼠（Microtus oeconomus）作为艾虎（Mustela eversmanni）的猎物选择对象,研究艾虎对猎物的选择性及艾虎捕杀猎物的行为时间分配.实验结果表明：艾虎对三种猎物的搜寻时间和搜寻频次基本一致,在搜寻猎物的过程中并非已知洞道系统中所存在的食物信息,是一种机会主义的捕食者;艾虎除了具有典型鼬科动物捕杀猎物的方法外,能够根据猎物的大小、活动性和反捕食能力采用不同的捕杀方法,在捕杀猎物的过程中首先捕杀高原鼢鼠,其致死部位全部为头部,其次捕杀高原鼠兔,其致死部位除了头部外,明显增加了颈部的比例,最后捕杀根田鼠,其致死部位主要是头部和胸部;艾虎在捕杀猎物的过程中,追击根田鼠的时间最多,追击鼢鼠的时间最少,而用于捕杀根田鼠的时间最少,用于捕杀鼢鼠的时间最多,艾虎捕杀高原鼠兔后单位时间内获得的能量值最大,其次为捕杀高原鼢鼠,而捕杀根田鼠后单位时间内活动的能量值最小.因此,在室内条件下艾虎对猎物的选择性主要依赖于其本身处理猎物的行为时间分配和猎物的反捕食行为,使单位时间内获得的能量值最大[动物学报 52（3）：437-443,2006].

青海歧穗大黄根部重金属元素特征

为对比青海高原境内不同生境歧穗大黄中七种重金属元素特征,用原子吸收仪进行了分析测试.结果表明,青海歧穗大黄根部Pb元素含量具有随着海拔高度增加而降低的变化特征.

Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia

The x- and y-type high molecular weight (HMW) glutenin subunits are conserved seed storage proteins in wheat and related species. Here we describe investigations on the HMW glutenin subunits from several Pseudoroegneria accessions. The electrophoretic mobilities of the HMW glutenin subunits from Pd. stipifolia, Pd tauri and Pd strigosa were much faster than those of orthologous wheat subunits, indicating that their protein size may be smaller than that of wheat subunits. The coding sequence of the Glu-1St1 subunit (encoded by the Pseudoroegneria stipifolia accession PI325181) was isolated, and found to represent the native open reading frame (ORF) by in vitro expression. The deduced amino acid sequence of Glu-1St1 matched with that determined from the native subunit by mass spectrometric analysis. The domain organization in Glu-1St1 showed high similarity with that of typical HMW glutenin subunits. However, Glu-1St1 exhibited several distinct characteristics. First, the length of its repetitive domain was substantially smaller than that of conventional subunits, which explains its much faster electrophoretic mobility in SDS-PAGE. Second, although the N-terminal domain of Glu-1St1 resembled that of y-type subunit, its C-terminal domain was more similar to that of x-type subunit. Third, the N- and C-terminat domains of Glu-1St1 shared conserved features with those of barley D-hordein, but the repeat motifs and the organization of its repetitive domain were more similar to those of HMW glutenin subunits than to D-hordein. We conclude that Glu-1St1 is a novel variant of HMW glutenin subunits. The analysis of Glu-1St1 may provide new insight into the evolution of HMW glutenin subunits in Triticeae species. (C) 2007 Elsevier Ltd. All rights reserved.

上下料直角坐标机器人机构研究

直角坐标机器人作为一种常用的工业机器人广泛应用于现代工业生产线中，其运动学和动力学特性关系整个生产线性能发挥。虚拟样机技术在复杂机械系统仿真中的成功应用不仅可以提高仿真精度，而且还可以缩短产品设计周期，对于工程实际具有重要的应用价值。通过虚拟样机技术，工程师可以通过机械系统运动仿真，在产品设计阶段发现产品设计中的潜在问题，并快速进行修改，减少了对于物理样机的依赖，这样不仅可以节省成本，缩短产品开发周期，而且还可以提高产品性能，增强产品竞争力。本文结合中科院沈阳自动化研究所现代装备研究设计中心的大型项目“激光拼焊生产线”，对生产线中从国外引进的上下料机器人进行研究分析，设计出一台可以满足“激光拼焊生产线”工业要求的上下料直角坐标机器人，来实现高性能上下料机器人的国产化。以设计出的上下料直角坐标机器人为研究对象，采用虚拟样机技术，以有限元法和多体系统动力学为理论基础，进行上下料直角坐标机器人机构的运动学和动力学分析仿真研究。其中研究了三维实体建模方法以及D-H方法，探讨了利用CAD/CAE软件SOLIDWORKS, ADAMS和ANSYS进行协同虚拟样机的建模、装配、数据共享、有限元静力分析以及刚柔耦合多体动力学仿真技术，研究成果对实际工程的运用具有一定的指导作用。本文主要的研究工作及其成果如下： 1根据工业生产线要求，确定高性能上下料直角坐标机器人总体机构设计方案，设计各个直线运动单元。 2利用CAD软件SOLIDWORKS对上下料直角坐标机器人进行3维实体建模，然后利用D-H方法，建立其运动学方程，最后利用MATLAB工具对运动学进行仿真。 3以有限元方法为基础，利用有限元分析软件ANSYS对上下料直角坐标机器人核心部件Y轴横梁静力分析，最后进行横梁结构优化。 4以多体系统动力学为基础，利用有限元软件ANSYS生成刚柔耦合多体动力学仿真所需的模态中性文件（MNF），同时结合运用ANSYS和ADAMS建立了上下料直角坐标机器人刚柔性耦合多体模型，并进行了动力学分析。

辽宁五龙金矿主成矿阶段成矿持续时限

在矿床地质研究的基础上，将辽宁五龙金矿的内生成矿作用从早到晚划分为5个成矿阶段。对其中具有工业意义的第二和第四主成矿阶段进行了石英流体包裹体Rb-Sr法测年，上述两个阶段年龄值分别为120±3Ma和112±1Ma，说明一个中、大型金矿的形成，其主成矿阶段可能要持续8Ma左右。Sr同位素的初始值表明，不同成矿阶段的成矿物质来自同一源区，且以壳源为主。

花溪河水碳同位素的季节变化及其环境意义

碳的全球生物地球化学循环是与全球变化相关的一系列重大国际合作计划的主要研究内容之一

铜同位素的分馏机制及其在矿床学研究中的应用

作为一种重要的成矿元素，铜广泛分布于不同地质体中，并广泛参与成岩成矿作用。近年来，由于同位素分析方法的改进和新一代多接收等离子质谱仪（MC-ICPMS）的应用，使得铜同位素的高精度测试成为现实，并已成为国际地学领域的一个前沿研究方向。铜同位素在自然界中具有较大的变化范围，δ^65Cu值介于-3．03‰～5．74‰之间。本文介绍和评述了铜同位素的分析方法和可能的分馏机制，并根据已发表数据，重点讨论了铜同位素组成与成矿温度、矿化阶段和成矿物质来源的关系。认为铜同位素有可能作为一种灵敏的地球化学示踪剂，对指示成矿物质来源、成矿作用过程和矿床形成机理具有重要作用。

Influência da cortina e do programa de luz no desempenho produtivo de frangos de corte e no consumo de energia elétrica.

2006

Valve surgery in active infective endocarditis: a simple score to predict in-hospital prognosis

Aims Surgery for infective endocarditis (IE) is associated with high mortality. Our objectives were to describe the experience with surgical treatment for IE in Spain, and to identify predictors of in-hospital mortality. Methods Prospective cohort of 1000 consecutive patients with IE. Data were collected in 26 Spanish hospitals. Results Surgery was performed in 437 patients (43.7%). Patients treated with surgery were younger and predominantly male. They presented fewer comorbid conditions and more often had negative blood cultures and heart failure. In-hospital mortality after surgery was lower than in the medical therapy group (24.3 vs 30.7%, p = 0.02). In patients treated with surgery, endocarditis involved a native valve in 267 patients (61.1%), a prosthetic valve in 122 (27.9%), and a pacemaker lead with no clear further valve involvement in 48 (11.0%). The most common aetiologies were Staphylococcus (186, 42.6%), Streptococcus (97, 22.2%), and Enterococcus (49, 11.2%). The main indications for surgery were heart failure and severe valve regurgitation. A risk score for in-hospital mortality was developed using 7 prognostic variables with a similar predictive value (OR between 1.7 and 2.3): PALSUSE: prosthetic valve, age ≥ 70, large intracardiac destruction, Staphylococcus spp, urgent surgery, sex [female], EuroSCORE ≥ 10. In-hospital mortality ranged from 0% in patients with a PALSUSE score of 0 to 45.4% in patients with PALSUSE score > 3. Conclusions The prognosis of IE surgery is highly variable. The PALSUSE score could help to identify patients with higher in-hospital mortality.