鱼类谷胱甘肽转移酶基因cDNA克隆及其序列分析

采用RT-PCR方法,从鲢、鲤、鲫3种鱼类肝脏总RNA中克隆出了谷胱甘肽转移酶Pi型(GST Pi)cDNA序列,推导了其编码的氨基酸序列。3种鱼类GST Pi的ORF全长627bp,编码208个氨基酸。翻译起始密码均为ATG,终止密码子均为TGA。鱼类与哺乳动物、两栖类爪蟾以及节肢动物丝虫之间GST Pi氨基酸序列相似度平均值分别为50%、33%、15%左右。5种鱼类之间的氨基酸序列相似度较大,其中鲤科鱼类之间平均为85%左右。我们以GST Pi为分子标记,用最大简约数法(MP)构建了13个物种的系统进

The lateral superjunction PSOI LIGBT and LDMOSFET

This paper presents a comparison between the superjunction LIGBT and the LDMOSFET in partial silicon-on-insulator (PSOI) technology in 0.18μm PSOI HV process. The superjunction drift region helps in achieving uniform electric field distribution in both structures but also contributes to the on-state current in the LIGBT. The superjunction LIGBT successfully achieves breakdown voltage (BV) of 210V with Rdson of 765mΩ.mm2. It exhibits reduced specific on-state resistance Rdson and higher saturation current (Idsat) for the same BV compared to a compatible lateral superjunction LDMOS in the same technology. © 2012 IEEE.

Simulating vision loss: What levels of impairment are actually represented?

Capability loss simulators give designers a brief experience of some of the functional effects of capability loss. They are an effective method of helping people to understand the impact of capability loss on product use. However, it is also important that designers know what levels of loss are being simulated and how they relate to the user population. The study in this paper tested the Cambridge Simulation Glasses with 25 participants to determine the effect of different numbers of glasses on a person's visual acuity. This data is also related to the glasses' use in usability assessment. A procedure is described for determining the number of simulator glasses with which the visual detail on a product is just visible. This paper then explains how to calculate the proportion of the UK population who would be unable to distinguish that detail.

微囊藻栅藻资源竞争的动力学过程Ⅰ.光能和磷营养的半饱和参数及其生长率动态

以Tilman提出的“资源竞争机制学说”为依据 ,在单因子 (光、磷 )限制条件下 ,测定并计算铜绿微囊藻 (Microcystisaeruginosa)和斜生栅藻 (Scenedesmusobliquus)的半饱和常数 ,以双因子交互作用模型预测它们的生长动态 .结果表明 :在光强为 10 .0～ 17.1μE和磷浓度为 3.10～ 2 0 .0 μmol的范围内 ,微囊藻的生长率大于栅藻的生长率 .说明磷的增加是微囊藻成为水华的充分条件 ,但不是必要条件 ,至少低光强是一个重要的作用因子 .

Seismic triggering of submarine slides in soft cohesive soil deposits

The geological profile of many submerged slopes on the continental shelf consists of normally to lightly overconsolidated clays with depths ranging from a few meters to hundreds of meters. For these soils, earthquake loading can generate significant excess pore water pressures at depth, which can bring the slope to a state of instability during the event or at a later time as a result of pore pressure redistribution within the soil profile. Seismic triggering mechanisms of landslide initiation for these soils are analyzed with the use of a new simplified model for clays which predicts realistic variations of the stress-strain-strength relationships as well as pore pressure generation during dynamic loading in simple shear. The proposed model is implemented in a finite element program to analyze the seismic response of submarine slopes. These analyses provide an assessment of the critical depth and estimated displacements of the mobilized materials and thus are important components for the estimation of submarine landslide-induced tsunamis. © 2003 Elsevier B.V. All rights reserved.

白鱀豚哨叫声的研究

于1996年1月，在中国科学院水生生物研究所白暨豚馆人工饲养池和湖北省石首市天鹅洲长江故道，使用频响范围分别为0－100kHz和0－16kHz的磁带记录系统，记录了一头成年雄性和一头成年雌性白暨豚的哨叫声。采用计算机信号分析系统详细分析了哨叫声的结构和声谱图，并与其它鲸类哨叫声进行比较。结果表明，白暨豚哨叫声的最大和最小频率平均值分别为5841Hz和4975Hz，频率变化幅度一般小于1000Hz。哨叫声平均持续时间为907ms。频率随时间变化不连续，且较平缓，起伏很少，有部分重叠现象。白暨豚哨叫声的结构与

Modeling of SFR cores with Serpent-DYN3D codes sequence

DYN3D reactor dynamics nodal diffusion code was originally developed for the analysis of Light Water Reactors. In this paper, we demonstrate the feasibility of using DYN3D for modeling of fast spectrum reactors. A homogenized cross sections data library was generated using continuous energy Monte-Carlo code Serpent which provides significant modeling flexibility compared with traditional deterministic lattice transport codes and tolerable execution time. A representative sodium cooled fast reactor core was modeled with the Serpent-DYN3D code sequence and the results were compared with those produced by ERANOS code and with a 3D full core Monte-Carlo solution. Very good agreement between the codes was observed for the core integral parameters and power distribution suggesting that the DYN3D code with cross section library generated using Serpent can be reliably used for the analysis of fast reactors. © 2012 Elsevier Ltd. All rights reserved.

在不同水硬度下铜对草鱼、鲢和大鳞泥鳅的急性毒性

实验以草鱼、鲢和大鳞泥鳅的鱼苗为材料,测定了水硬度对铜的急性毒性的影响,水硬度在255、141、37和14mg/L(以 CaCO_3计)时铜对草鱼的48小时 LC_(50)分别是519、354、91和76μg/L,96小时 LC_(50)则分别为460、307、67和39μg/L.在同样的硬度条件下,对鲢的48小时 LC_(50)是452、393、71和40μg/L,96小时 LC_(50)分别为452、296、41和31μg/L.大鲢泥鳅鱼苗在硬度260、137、36和10μg/L(以 CaCO_3计)

Sparse recovery of complex phase-encoded velocity images using iterative thresholding

In this paper we propose a new algorithm for reconstructing phase-encoded velocity images of catalytic reactors from undersampled NMR acquisitions. Previous work on this application has employed total variation and nonlinear conjugate gradients which, although promising, yields unsatisfactory, unphysical visual results. Our approach leverages prior knowledge about the piecewise-smoothness of the phase map and physical constraints imposed by the system under study. We show how iteratively regularizing the real and imaginary parts of the acquired complex image separately in a shift-invariant wavelet domain works to produce a piecewise-smooth velocity map, in general. Using appropriately defined metrics we demonstrate higher fidelity to the ground truth and physical system constraints than previous methods for this specific application. © 2013 IEEE.

Molecular cloning of the viperin gene and its promoter region from the mandarin fish Siniperca chuatsi

A viperin gene has been cloned from the mandarin fish (Siniperca chuatsi). From the first transcription initiation site, the mandarin fish viperin gene extends 3163 nucleotides to the end of the 3' untranslated region, and it contains six exons and five introns. The open reading frame of the viperin transcript has 1062 nucleotides which encode a 354 amino acid peptide. The amino acid sequence of mandarin fish viperin shows high identities with its homologues in teleosts and mammals except for the first 70 amino acids. A characteristic feature in the viperin promoter region was the presence of five putative ICSBP (IRF8) binding sites and one IRFI binding site. The viperin gene expressed mainly in lymphoid tissues before stimulation, but its expression can be examined in almost all the organs investigated after stimulation with virus or Poly I:C. The expression pattern and promoter sequence may be considered as the indirect evidence that the transcription of viperin is regulated by interferons or interferon induced genes. (C) 2004 Elsevier B.V. All rights reserved.

Integrated ZnO surface acoustic wave microfluidic components for biosensor

This paper describes the novel nanocrystalline film ZnO surface acoustic wave devices, which demonstrate their great potential for the portable disease diagnostic system with integrated functions of microfluidic transport, mixing and biosensing. The devices can be easily integrated with electronic control circuitry and fabricated with low temperature process on Si, glass or even polymer substrates. The liquid convection and internal streaming patterns was easily induced by acoustic wave at signal voltages. With further increase in applied voltage to above 20V, the liquid droplet was pushed forward. Immunoreaction-based bio-detection PSA/ACT, all based on SAW devices on thin film piezoelectric ZnO on Si substrate was demonstrated. © 2009 CBMS.

A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish

Oocyte maturation and egg fertilization in both vertebrates and invertebrates are marked by orchestrated cytoplasmic translocation of secretory vesicles known as cortical granules. It is thought that such redistribution of cellular content is critical for asymmetrical cell division during early development, but the mechanism and regulation of the process is poorly understood. Here we report the identification, purification and cDNA cloning of a C-type lectin from oocytes of a freshwater fish species gibel carp (Carassius auratus gibelio). The purified protein has been demonstrated to have lectin activity and to be a Ca2+-dependent C-type lectin by hemagglutination activity assay. Immunocytochemistry revealed that the lectin is associated with cortical granules, gradually translocated to the cell surface during oocyte maturation, and discharged to the egg envelope upon fertilization. Interestingly, the lectin becomes phosphorylated on threonine residues upon induction of exocytosis by fertilization and returns to its original state after morula stage of embryonic development, suggesting that this posttranslational modification may represent a critical molecular switch for early embryonic development. (C) 2003 Elsevier Inc. All rights reserved.

Computer assisted peak recognition for polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) in environmental samples

A software has been developed for the peak recognition of 136 polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) after high resolution gas chromatography coupled with mass spectrometry (HRGC/HRMS). Based on the retention times of C-13 labelled 2,3,7,8-substituted PCDD/F internal standards, the retention times of all PCDD and PCDF can be calibrated automatically and accurately. Therefore, it is very convenient to identify the peaks by comparing the retention of samples and the calibrated retention times of their chromatograms. Hence, this approach is very significant because it is impossible to obtain always a standard chromatogram and PCDD/F analysis are very expensive and time consuming. The calibration results can be transferred to Excel for calculation. The approach is a first step to store costly and environmentally relevant data for future application.

Peculiarity of constant photocurrent method for silicon films with mixed amorphous-nanocrystalline structure

The results of conductivity, photoconductivity and constant photocurrent method absorption measurements by DC and AC methods in hydrogenated silicon films with mixed amorphous-nanocrystalline structure are presented. A series of diphasic silicon films was deposited by very high frequency plasma enhanced chemical vapor deposition technique, using different hydrogen dilution ratios of silane. The increase of hydrogen dilution ratio results in five orders of magnitude increase of conductivity and a sharp increase of grain volume fraction. The comparison of the absorption spectra obtained by DC and AC methods showed that they are similar for silicon films with the predominantly amorphous structure and films with high grain volume fraction. However we found a dramatic discrepancy between the absorption spectra obtained by DC and AC constant photocurrent methods in silicon films deposited in the regime of the structure transition from amorphous to nanocrystalline state. AC constant photocurrent method gives higher absorption coefficient than DC constant photocurrent method in the photon energy range of 1.2-1.7 eV. This result indicates the possibility of crystalline grains contribution to absorption spectra measured by AC constant photocurrent method in silicon films with intermediate crystalline grain volume fraction. (c) 2008 Published by Elsevier B.V.