大蹼铃蟾缓激肽及其制备方法和其基因

本发明涉及大蹼铃蟾缓激肽及其制备方法和其基因，属于生物医学领域。其缓激肽为从中国两栖类动物大蹼铃蟾(Bombina maxima)皮肤分泌物中分离得到的由19个氨基酸组成的一种单链多肽，分子量2180，等电点11.7，多肽氨基酸全序列一级结构为：DLPKINRKGPRPPGFSPFR。制备方法是收集大蹼铃蟾皮肤分泌物，离心去除沉淀、冷冻干燥后，经凝胶过滤、高压液相反相柱层析分离纯化后即得到。编码大蹼铃蟾缓激肽基因核苷酸序列由cDNA由829个核苷酸组成，编码成熟大蹼铃蟾缓激肽为第140-196，224-280，308-364，392-448，476-532，560-616位6个核苷酸重复单元片断，从两栖类动物皮肤中得到的缓激肽基因作为基因工程制备缓激肽的应用。

Report of the Fisheries Statistics Working Group Meeting, Medan, Indonesia, 19-20 March, 2012

Statistics and data overviews from the eight member countries. Southeast Asian Fisheries Development Center(SEAFDEC)overview and activities since 2010. Statistics working group recommendations and workplan

Report of the workshop on ecosystem characterisation of Indian coastal waters, Dona Paula, Goa, India, 20-22 October, 2014

The goals of the workshop were to: conduct an interactive workshop for characterizing Indian coastal ecosystems; verification of the ecosystem characterisation report for the East coast of India; and development of ecosystem characterisation for the west coast of India based on ecological/biophysical systems.

Report of the essential EAFM training course, Chittagong, Bangladesh, 12-20 November, 2014

Participants were exposed to concepts and information about Ecosystem Approach to Fisheries Management (EAFM) using a structured, participatory method of delivery. The learning strategy involved specifically designed exercises, using real examples, to consolidate learning. Daily monitoring and reviews were conducted together with pre-and post-course assessment.

Report of the NBFGR-BOBLME Indian mackerel genetics harmonisation training workshop, Kochi, India, 20-27 August, 2013

This workshop was held at the National Bureau of Fish Genetic Resources and followed on from the Indian mackerel Working group meeting in Colombo (28-29 May, 2012). Activities included; DNA extraction; PCR (Polymerase Chain Reaction) for microsatellites; genotyping microsatellites; data analysis; emerging technologies; and an action plan

Report of the pilot Essential EAFM course, Samut Prakan, Thailand, 20-25 January, 2014

This course, co-hosted by South East Asian Fisheries Development Centre (SEAFDEC), was a refined version of the pilot course held in Malaysia in June 2013. Participants were exposed to concepts and information about Ecosystem Approach to Fisheries Management (EAFM) using a structured, participatory method of delivery. The learning strategy involved specifically designed exercises, using real examples, to consolidate learning. Daily monitoring and reviews were conducted together with pre-and post-course assessment.

Report of the training course on integrated coastal management in the Bay of Bengal, Chonburi, Thailand, 20-24 October, 2014

Participants consisted of 25 middle and junior level personnel from BOBLME countries. Modules included: Integrated Coastal Management (ICM) concept and principles; ICM development and implementation; indicators of good practice; and action planning.

20 Gb/s QPSK transmission using an electro-optically modulated vertical-cavity surface-emitting laser

20 Gb/s QPSK transmission over 100 m of OM3 fibre using an EOM VCSEL under QPSK modulation is reported. Bit-error-ratio measurements are carried out to express the quality of the transmission scheme. © 2011 OSA.

RESERPINE IMPAIRS SPATIAL WORKING-MEMORY PERFORMANCE IN MONKEYS - REVERSAL BY THE ALPHA-2-ADRENERGIC AGONIST CLONIDINE

Repeated daily treatment with the catecholamine-depleting agent, reserpine, dramatically reduced performance on the delayed response task, a test of spatial working memory that depends upon the integrity of the prefrontal cortex. Delayed response performance fell from an average of 27.2/30 trials correct before reserpine treatment to an average of 20.4/30 trials correct after repeated reserpine administration. Injection of the alpha2-adrenergic agonist, clonidine (0.0001-0.05 mg/kg), to chronic reserpine-treated monkeys significantly restored performance on the delayed response task; performance after an optimal dose averaged 27.8/30 trials correct. Clonidine's beneficial effects on delayed response performance were longlasting; monkeys remained improved for more than 24 h after a single clonidine injection. The finding that clonidine is efficacious in reserpinized animals supports the hypothesis that alpha2-adrenergic agonists improve cognitive function through actions at postsynaptic, alpha2-adrenergic receptors on non-adrenergic cells. In contrast to the delayed response task, reserpine had little effect on performance of a visual discrimination task, a reference memory task which does not depend on the prefrontal cortex. These results emphasize the importance of postsynaptic alpha2-adrenergic mechanisms in the regulation of working memory,

High prevalence of HIV-1 and hepatitis C virus coinfection among injection drug users in the southeastern region of Yunnan, China

The southeastern region of Yunnan province is a key site for drug trafficking and HIV-1 infection spread from the west of Yunnan and Laos to southeastern China. To investigate the prevalence of HIV-1 infection and hepatitis C virus (HCV) coinfection among injection drug users (IDUs) in southeastern Yunnan, three cohorts of 285 addicts, including 242 IDUs and 43 oral drug users, living in the cities of Gejiu and Kaiyuan and the county of Yanshan were studied. HIV-1 and HCV infections were detected by enzyme-linked immunosorbent assay and/or polymerase chain reaction. Data on the age, sex, risk behavior, drug use history, employment, ethnic background, and marriage status were obtained by interview. The overall prevalence of HIV-1 infection was 71.9%. The rate of HCV coinfection among 138 HIV-1-infected IDUs was 99.3%. Most HIV-infected IDUs were 20 to 35 years old (86.7%) and were ethnic Han (75.9%), suggesting that the epidemic in Yunnan is no longer confined to non-Han ethnic minorities, HIV prevalence in female IDUs (81.2%) was significantly higher than in male IDUs (68.2%) (p <.05). The prevalence of HIV infection reached 68.4% after 1 year of injection drug use. Needle/syringe sharing is the major high risk factor for the spread of HIV-1 and HCV infections. Large-scale educational campaigns are urgently needed to reduce the spread of HIV and HCV infection in these regions.