Padronização da coaglutinação na preparação de ácidos nucléicos do parvovírus canino e vírus da cinomose para diagnóstico molecular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Otimização, validação e aplicação de método para análise de diuron e seus metabólitos em urina de ratos utilizando SPE e HPLC-DAD

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Exposição à mistura de pesticidas em baixas doses: validação de método e aplicação a tecido adiposo de animais de experimentação (ratos Lewis)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de um sistema de injeção em fluxo para a determinação seqüencial de fosfito e fosfato em amostras agroindustriais por espectrofotometria

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nanopartículas de óxido de ítrio dopado com Eu3+ contendo prata

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Nanocompósito de alumina com inclusão nonométricas de zircônia para infraestruturas de próteses odontológicas livres de metal: avaliação de prosperidades mecânicas e análise microestrutural. -

Pós-graduação em Reabilitação Oral - FOAR

Desenvolvimento de vasos digestores de amostra para microondas doméstico

The application of microwave radiation on the sample preparation has been expanding increasingly in areas involving decomposition by wet and dry roads, fusion, extraction, acceleration of chemical reactions, for example. Currently, the use of microwave ovens for analytical purposes are recognized for having excellent performance for organic and inorganic samples. In the international market there are several kinds of microwaves oven which adapt the varied purposes, however yet with elevated prices which incapacitate your use as routine equipment in laboratory. Thus, many researchers have been choosing for developing own projects of microwaves oven production or to use domestic oven for the laboratory, with or without adaptations. For the evaluation of the proposed method was used in the Kjeldahl methodology for determining total nitrogen in samples of crude protein, using a domestic microwave oven and a digester pot made up in TeflonTM and distillation by steam. Were made to adapt and characterization of a domestic microwave oven, the confeccion vessel digester and the metal support for the vessel. The accuracy of the proposed method was confirmed by comparison of two methods, the standard method for conventional heating and by the proposed method, with heating by microwave radiation through the calculated values of relative standard deviation analysis

Analysis of fluoxetine and norfluoxetine in human plasma by HPLC-UV using a high purity C18 silica-based SPE sorbent

This paper reports on the development and validation of a simple and sensitive method that uses solid phase extraction (SPE) and liquid chromatography with ultraviolet detection to analyze fluoxetine (FLX) and norfluoxetine (NFLX) in human plasma samples. A lab-made C18 SPE phase was synthesized by using a sol–gel process employing a low-cost silica precursor. This sorbent was fully characterized by nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) to check the particles' shape, size and C18 functionalization. The lab-made C18 silica was used in the sample preparation step of human plasma by the SPE-HPLC-UV method. The method was validated in the 15 to 500 ng mL 1 range for both FLX and NFLX using a matrix matched curve. Detection limits of 4.3 and 4.2 ng mL 1 were obtained for FLX and NFLX, respectively. The repeatability and intermediary precision achieved varied from 7.6 to 15.0% and the accuracy ranged from 14.9 to 9.1%. The synthesized C18 sorbent was compared to commercial C18 sorbents. The average recoveries were similar (85–105%), however the lab-made C18 silica showed fewer interfering peaks in the chromatogram. After development and validation, the method using the lab-made C18 SPE was applied to plasma samples of patients under FLX treatment (n ¼ 6). The concentrations of FLX and NFLX found in the samples varied from 46.8–215.5 and 48.0–189.9 ng mL 1 , respectively.

Peptídeos cíclicos hepatotóxicos: MALDI-TOF como uma ferramenta analítica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Development of a new clean methodology with ultrasound-assisted extraction for analysis of sodium in pet foods

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Direct determination of chromium in empty medicine capsules by tungsten coil atomic emission spectrometry

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Single precursor route to efficient cobalt sulphide counter electrodes for dye sensitized solar cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Rapid determination of 12 antibiotics and caffeine in sewage and bioreactor effluent by online column-switching liquid chromatography/tandem mass spectrometry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sulfamethoxazole and ciprofloxacin removal using a horizontal-flow anaerobic immobilized biomass reactor

The antibiotics sulfamethoxazole (SMTX) and ciprofloxacin (CIP) are commonly used in human and veterinary medicine, which explains their occurrence in wastewater. Anaerobic reactors are low-cost, simple and suitable technology to wastewater treatment, but there is a lack of studies related to the removal efficiency of antibiotics. To overcome this knowledge gap, the objective of this study was to evaluate the removal kinetics of SMTX and CIP using a horizontal-flow anaerobic immobilized biomass reactor. Two different concentrations were evaluated, for SMTX 20 and 40 μg L(-1); for CIP 2.0 and 5.0 μg L(-1). The affluent and effluent analysis was carried out in liquid chromatography/tandem mass spectrometry (LC-MS/MS) with the sample preparation procedure using an off-line solid-phase extraction. This method was developed, validated and successfully applied for monitoring the affluent and effluent samples. The removal efficiency found for both antibiotics at the two concentrations studied was 97%. Chemical oxygen demand (COD) exhibited kinetic constants that were different from that observed for the antibiotics, indicating the absence of co-metabolism. Also, though the antibiotic concentration was increased, there was no inhibitory effect in the removal of COD and antibiotics.

Determinação de fósforo em biodiesel utilizando eletrodo quimicamente modificado com nanopartículas de ferro

Once petroleum is na exhaustible source of energy, alternative fuels are having more prominence. A much discussed option for replacing fossil fuels is the use of biofuels derived from oils or fats, especially biodiesel. The biodiesel preparation is through a reaction named transesterification, a reaction of triglycerides with a short chain alcohol with a catalyst, producing a mixture of fatty acid esters and glycerol. According to ANP (National Petroleum Agency) specifications, biodiesel can have contaminants due to the catalyst or oil used on its synthesis, such as phosphorus, wich can damage the catalytic converter and cause significant increase in the particles emission. This project aims to develop na alternative method using chemically modified electrodes with iron nanoparticles for determination of phosphorus in biodiesel. For the formation of the iron nanoparticles film on the surface of a glassy carbon electrode, was used a iron sulfate solution. The film was formed after 10 successive cycles, with a scanning speed of 50 mV s-1 and a potential range of -0,9 to -1,25 V. To reduce possible oxides on the surface and activate the electrode, it has been subjected to a cathodic polarization with a potential of -1,25 V for 15 minutes in a sodium hydroxide solution. In cyclic voltammograms obtained in the study of the speed of scanning, there is an increase in the intensity of the anodic and cathodic current peaks. The cathodic peak current varied linearly with the square root of scan rate, showing that the electrode is controlled by diffusion. After successive additions of phosphate there is a linear variation in the current peak in the concentration range of 1,0 x 10-7 a 1,0 x 10-6 mol L-1. To determine if the concentration of phosphorus in real sample, the method of adding standard was used by adding aliquots of phosphate ions in the solution containing soy biodiesel extracted with ....