Karyotype analysis of seven species of the tribe lophiohylini (Hylinae, Hylidae, Anura), with conventional and molecular cytogenetic techniques

Few species of the tribe Lophiohylini have been karyotyped so far, and earlier analyses were performed mainly with standard staining. Based on the analysis of seven species with use of routine banding and molecular cytogenetic techniques, the karyotypes were compared and the cytogenetic data were evaluated in the light of the current phylogenies. A karyotype with 2n = 24 and NOR in the chromosome 10 detected by Ag-impregnation and FISH with an rDNA probe was shared by Aparasphenodon bokermanni Miranda-Ribeiro, 1920, Itapotihyla langsdorffii (Duméril and Bibron, 1841), Trachycephalus sp., T. mesophaeus (Hensel, 1867), and T. typhonius (Linnaeus, 1758). Phyllodytes edelmoi Peixoto, Caramaschi et Freire, 2003 and P. luteolus (Wied-Neuwied, 1824) had reduced the diploid number from 2n = 24 to 2n = 22 with one of the small-sized pairs clearly missing, and NOR in the large chromosome 2, but the karyotypes were distinct regarding the morphology of chromosome pairs 4 and 6. Based on the cytogenetic and phylogenetic data, it was presumed that the chromosome evolution occurred from an ancestral type with 2n = 24, in which a small chromosome had been translocated to one or more unidentified chromosomes. Whichever hypothesis is more probable, other rearrangements should have occurred later, to explain the karyotype differences between the two species of Phyllodytes Wagler, 1830. The majority of the species presented a small amount of centromeric C-banded heterochromatin and these regions were GC-rich. The FISH technique using a telomeric probe identified the chromosome ends and possibly (TTAGGG)n-like sequences in the repetitive DNA out of the telomeres in I. langsdorffii and P. edelmoi. The data herein obtained represent an important contribution for characterizing the karyotype variability within the tribe Lophiohylini scarcely analysed so far. © Simone Lilian Gruber et al.

A new snake of the genus Helicops Wagler, 1830 (Dipsadidae, Xenodontinae) from Brazil

We describe a new species of Helicops from the southern Amazon Basin in the state of Mato Grosso, Brazil. It differs from all congeners by having dorsal scales in 21/21/19 rows in males and 23/21/19 rows in females, subcaudal keels, a banded dorsal color pattern, and 14-19 ventral blotches. Besides presenting information on the lepidosis and morphometric variation, we also describe the hemipenis and discuss the known distribution of the new species. © 2013 by The Herpetologists' League, Inc.

High chromosome variability and the presence of multivalent associations in buthid scorpions

In this study, we investigated the mitotic and meiotic chromosomes of 11 Buthidae scorpion species, belonging to three genera (Ananteris, Rhopalurus and Tityus), to obtain detailed knowledge regarding the mechanisms underlying the intraspecific and/or interspecific diversity of chromosome number and the origin of the complex chromosome associations observed during meiosis. The chromosomes of all species did not exhibit a localised centromere region and presented synaptic and achiasmatic behaviour during meiosis I. Spermatogonial and/or oogonial metaphase cells of these buthids showed diploid numbers range from 2n = 6 to 2n = 28. In most species, multivalent chromosome associations were observed in pachytene and postpachytene nuclei. Moreover, intraspecific variability associated with the presence or absence of chromosome chains and the number of chromosomes in the complex meiotic configurations was observed in some species of these three genera. Silver-impregnated cells revealed that the number and location of nucleolar organiser regions (NORs) remained unchanged despite extensive chromosome variation; notably, two NORs located on the terminal or subterminal chromosome regions were commonly observed for all species. C-banded and fluorochrome-stained cells showed that species with conspicuous blocks of heterochromatin exhibited the lowest rate of chromosomal rearrangement. Based on the investigation of mitotic and meiotic cells, we determined that the intraspecific variability occurred as a consequence of fission/fusion-type chromosomal rearrangements in Ananteris and Tityus species and reciprocal translocation in Rhopalurus species. Furthermore, we verified that individuals presenting the same diploid number differ in structural chromosome organisation, giving rise to intraspecific differences of chromosome association in meiotic cells (bivalent-like elements or chromosome chains). © 2013 Springer Science+Business Media Dordrecht.

Formas de aplicación de zinc para las plantas de maíz que crecen en Oxisol: Efectos sobre el suelo, la nutrición de las plantas y la productividad

The way of applying zinc can influence the zinc uptake and productivity of crops, especially cereals that have high demand for this nutrient. The aim of this study is to evaluate methods of Zn application on soil, nutritional status and productivity of maize. For this, an experiment was undertaken at FCAV/UNESP, Jaboticabal-SP, in Oxisol clay (DTPA on Zn: 0.5 mg dm-3) with maize (hybrid Simple Impact), from December through May 2009. Nine treatments with three doses of Zn in soil banded application (in furrows) and three doses of Zn by incorporation in soil (0-20 cm depth), foliar application, seed application and control (no Zn). The treatments were arranged in a randomized block design with four replications. Regardless of the method, Zn application promoted higher contents of this micronutrient in soil and higher accumulation in the shoots as well as increasing Zn in the maize grain. However, it did not affect the nutritional status and yield of the maize. The Zn application in the soil resulted in a greater Zn uptake by plants and maize yield, compared to Zn application in the plant by seed or foliar.

Caracterização estrutural da porção sul do greenstone belt de Guarinos, GO

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Geologia estrutural aplicada às rochas ornamentais na Pedreira Knawa, Cláudio (MG)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Gênese dos corpos de sulfeto maciço hidrotermal e platinóides associados no depósito de Fortaleza de Minas (MG)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

As formações ferríferas bandadas de Piumhi: geologia, petrografia e caracterização tecnológica

Pós-graduação em Geologia Regional - IGCE

Avaliação dos granitos Giallo São Francisco Real, Branco Dallas e Branco Marfim, do muncípio de Barra de São Francisco/ES para aplicação como rocha ornamental e de revestimento

Pós-graduação em Geologia Regional - IGCE

A administração de GH atenua o desenvolvimento de fibrose miocádica em ratos com insuficiência cardíaca secundária a estenose aórtica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Tomografia computadorizada de feixe cônico para avaliação do tratamento da classe II divisão 1ª com o aparelho Herbst no espaço aéreo faríngeo

Pós-graduação em Ciências Odontológicas - FOAR

Família e obesidade: um estudo psicanalítico a partir do discurso de pacientes submetidos a gastroplastia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Área de vida da choca-do-planalto Thamnophilus pelzelni Hellmayr, 1924 (Passeriformes: Thamnophilidae) na estação ecológica de Jataí, Luiz Antônio, SP

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Ecologia molecular de fungos patogênicos onygenales em animais silvestres do interior do estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diagênese e alteração hidrotermal em rochas sedimentares da formação Corumbataí, Permiano Superior, Mina Granusso, Cordeirópolis/SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)